Exogenous and endogenous HDAC inhibitor effects in Rubinstein-Taybi syndrome models

ABSTRACTRubinstein-Taybi syndrome (RSTS) is an autosomal dominant disorder with specific clinical signs and neurodevelopmental impairment. The two known proteins altered in the majority of RSTS patients are the histone acetylation regulators CBP and p300. For assessing possible ameliorative effects of exogenous and endogenous HDAC inhibitors (HDACi), we exploited in vivo and in vitro RSTS models. First, HDACi effects were tested on Drosophila melanogaster, showing molecular rescue. In the same model, we observed a shift in gut microbiota composition. We then studied HDACi effects in RSTS cell lines compared to healthy donor cells. We observed patients-specific molecular rescue of acetylation defects at subtoxic concentrations. Finally, we assessed commensal gut microbiota composition in a cohort of RSTS patients compared to healthy siblings. Intriguingly, we observed a significant depletion in butyrate-producing bacteria in RSTS patients. In conclusion, this study reports the possibility of modulating acetylation equilibrium by HDACi treatments and the importance of microbiota composition in a chromatinopathy.

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Plant polyphenols alter a pathway of energy metabolism by inhibiting fecal Bacteroidetes and Firmicutes in vitro

Food & Function ◽

10.1039/c5fo01438g ◽

2016 ◽

Vol 7 (3) ◽

pp. 1501-1507 ◽

Cited By ~ 36

Author(s):

Bin Xue ◽

Jinli Xie ◽

Jiachen Huang ◽

Long Chen ◽

Lijuan Gao ◽

...

Keyword(s):

Energy Metabolism ◽

Gut Microbiota ◽

Microbiota Composition ◽

Faecal Microbiota ◽

Plant Polyphenols ◽

Gut Microbiota Composition

This study investigated the effect of plant polyphenols on faecal microbiota metabolizing oligosaccharides. The results show that plant polyphenols can change the pathway of degrading FOS or even energy metabolism in vivo by altering gut microbiota composition.

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Fermentation properties and potential prebiotic activity of Bimuno®galacto-oligosaccharide (65 % galacto-oligosaccharide content) onin vitrogut microbiota parameters

British Journal Of Nutrition ◽

10.1017/s0007114516002269 ◽

2016 ◽

Vol 116 (3) ◽

pp. 480-486 ◽

Cited By ~ 17

Author(s):

Roberta Grimaldi ◽

Jonathan R. Swann ◽

Jelena Vulevic ◽

Glenn R. Gibson ◽

Adele Costabile

Keyword(s):

Gut Microbiota ◽

Time Course ◽

Microbiota Composition ◽

Small Molecular Weight ◽

Food Industries ◽

H Nmr ◽

Prebiotic Oligosaccharides ◽

Gut Microbiota Composition

AbstractPrebiotic oligosaccharides have the ability to generate important changes in the gut microbiota composition that may confer health benefits to the host. Reducing the impurities in prebiotic mixtures could expand their applications in food industries and improve their selectivity and prebiotic effect on the potential beneficial bacteria such as bifidobacteria and lactobacilli. This study aimed to determine thein vitropotential fermentation properties of a 65 % galacto-oligosaccharide (GOS) content Bimuno®GOS (B-GOS) on gut microbiota composition and their metabolites. Fermentation of 65 % B-GOS was compared with 52 % B-GOS in pH- and volume-controlled dose–response anaerobic batch culture experiments. In total, three different doses (1, 0·5 and 0·33 g equivalent to 0·1, 0·05 and 0·033 g/l) were tested. Changes in the gut microbiota during a time course were identified by fluorescencein situhybridisation, whereas small molecular weight metabolomics profiles and SCFA were determined by1H-NMR analysis and GC, respectively. The 65 % B-GOS showed positive modulation of the microbiota composition during the first 8 h of fermentation with all doses. Administration of the specific doses of B-GOS induced a significant increase in acetate as the major SCFA synthesised compared with propionate and butyrate concentrations, but there were no significant differences between substrates. The 65 % B-GOS in syrup format seems to have, in all the analysis, an efficient prebiotic effect. However, the applicability of such changes remains to be shown in anin vivotrial.

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Effects of phlorotannin and polysaccharide fractions of brown seaweed Silvetia compressa on human gut microbiota composition using an in vitro colonic model

Journal of Functional Foods ◽

10.1016/j.jff.2021.104596 ◽

2021 ◽

Vol 84 ◽

pp. 104596

Author(s):

Benjamín Vázquez-Rodríguez ◽

Liliana Santos-Zea ◽

Erick Heredia-Olea ◽

Laura Acevedo-Pacheco ◽

Arlette Santacruz ◽

...

Keyword(s):

Gut Microbiota ◽

Brown Seaweed ◽

Microbiota Composition ◽

Human Gut ◽

Human Gut Microbiota ◽

Gut Microbiota Composition

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Colonisation potential of plantaricin-producing Lactobacillus plantarum SF9C andS-layer-carrying Lactobacillus brevis SF9B among gut microbiota

10.21203/rs.2.23400/v2 ◽

2020 ◽

Author(s):

Katarina Butorac ◽

Martina Banic ◽

Jasna Novak ◽

Andreja Leboš Pavunc ◽

Ksenija Uroic ◽

...

Keyword(s):

Gut Microbiota ◽

Lactobacillus Plantarum ◽

Lactobacillus Brevis ◽

Illumina Miseq ◽

Rrna Gene ◽

Microbiota Composition ◽

Combined Application ◽

Probiotic Potential ◽

Gut Microbiota Composition

Abstract Background: The influence of an S-layer-carrying strain Lactobacillus brevis SF9B and a plantaricin-producing strain Lactobacillus plantarum SF9C on the gut microbiota composition was evaluated in the rats. Considering the probiotic potential of Lb. brevis SF9B, this study aimed to examine the antibacterial activity of Lb. plantarum SF9C and potential for their in vivo colonisation, which could be the basis for the investigation of their synergistic functionality. Results: A plantaricin-encoding cluster was identified in Lb. plantarum SF9C, a strain which efficiently inhibited the growth of Listeria monocytogenes ATCC®19111™ and Staphylococcus aureus 3048. Contrary to the plantaricin-producing SF9C strain, the S-layer-carrying SF9B strain excluded Escherichia coli 3014 and Salmonella enterica serovar Typhimurium FP1 from adhesion to Caco-2 cells. Finally, DGGE analysis of the V2-V3 region of the 16S rRNA gene confirmed the transit of two selected lactobacilli through the gastrointestinal tract (GIT). Microbiome profiling via the Illumina MiSeq platform revealed the prevalence of Lactobacillus spp. in the gut microbiota of rats suggesting their colonisation potential in GIT.Conclusion: The combined application of Lb. plantarum SF9C and Lb. brevis SF9B could influence the intestinal microbiota composition, which is reflected through the increased abundance of Lactobacillus genus, but also through altered abundances of other bacterial genera, either in the model of healthy or aberrant microbiota of rats. The obtained results contributed to the functional aspects of SF9C and SF9B strains which could be incorporated in the probiotic-containing functional foods and therefore have a beneficial influence on the gut microbiota composition.

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Effects of Dietary Fibres on Acute Indomethacin-Induced Intestinal Hyperpermeability in the Elderly: A Randomised Placebo Controlled Parallel Clinical Trial

Nutrients ◽

10.3390/nu12071954 ◽

2020 ◽

Vol 12 (7) ◽

pp. 1954

Author(s):

John-Peter Ganda Mall ◽

Frida Fart ◽

Julia A. Sabet ◽

Carl Mårten Lindqvist ◽

Ragnhild Nestestog ◽

...

Keyword(s):

Gut Microbiota ◽

Barrier Function ◽

Intestinal Barrier ◽

The Elderly ◽

Intestinal Barrier Function ◽

Elderly Subjects ◽

Microbiota Composition ◽

Dietary Fibres ◽

Gut Microbiota Composition

The effect of dietary fibres on intestinal barrier function has not been well studied, especially in the elderly. We aimed to investigate the potential of the dietary fibres oat β-glucan and wheat arabinoxylan to strengthen the intestinal barrier function and counteract acute non-steroid anti-inflammatory drug (indomethacin)-induced hyperpermeability in the elderly. A general population of elderly subjects (≥65 years, n = 49) was randomised to a daily supplementation (12g/day) of oat β-glucan, arabinoxylan or placebo (maltodextrin) for six weeks. The primary outcome was change in acute indomethacin-induced intestinal permeability from baseline, assessed by an in vivo multi-sugar permeability test. Secondary outcomes were changes from baseline in: gut microbiota composition, systemic inflammatory status and self-reported health. Despite a majority of the study population (85%) showing a habitual fibre intake below the recommendation, no significant effects on acute indomethacin-induced intestinal hyperpermeability in vivo or gut microbiota composition were observed after six weeks intervention with either dietary fibre, compared to placebo.

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Effects of Rich in Β-Glucans Edible Mushrooms on Aging Gut Microbiota Characteristics: An In Vitro Study

Molecules ◽

10.3390/molecules25122806 ◽

2020 ◽

Vol 25 (12) ◽

pp. 2806 ◽

Cited By ~ 1

Author(s):

Evdokia K. Mitsou ◽

Georgia Saxami ◽

Emmanuela Stamoulou ◽

Evangelia Kerezoudi ◽

Eirini Terzi ◽

...

Keyword(s):

Gut Microbiota ◽

Wheat Straw ◽

In Vitro Study ◽

Short Chain Fatty Acids ◽

Edible Mushrooms ◽

Elderly Subjects ◽

Microbiota Composition ◽

The Impact ◽

Gut Microbiota Composition

Alterations of gut microbiota are evident during the aging process. Prebiotics may restore the gut microbial balance, with β-glucans emerging as prebiotic candidates. This study aimed to investigate the impact of edible mushrooms rich in β-glucans on the gut microbiota composition and metabolites by using in vitro static batch culture fermentations and fecal inocula from elderly donors (n = 8). Pleurotus ostreatus, P. eryngii, Hericium erinaceus and Cyclocybe cylindracea mushrooms derived from various substrates were examined. Gut microbiota composition (quantitative PCR (qPCR)) and short-chain fatty acids (SCFAs; gas chromatography (GC)) were determined during the 24-h fermentation. P. eryngii induced a strong lactogenic effect, while P. ostreatus and C. cylindracea induced a significant bifidogenic effect (p for all <0.05). Furthermore, P. eryngii produced on wheat straw and the prebiotic inulin had comparable Prebiotic Indexes, while P. eryngii produced on wheat straw/grape marc significantly increased the levels of tested butyrate producers. P. ostreatus, P. eryngii and C. cylindracea had similar trends in SCFA profile; H. erinaceus mushrooms were more diverse, especially in the production of propionate, butyrate and branched SCFAs. In conclusion, mushrooms rich in β-glucans may exert beneficial in vitro effects in gut microbiota and/or SCFAs production in elderly subjects.

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2581. An Invertebrate Model to Study Gut Microbiome Dysbiosis

Open Forum Infectious Diseases ◽

10.1093/ofid/ofz360.2259 ◽

2019 ◽

Vol 6 (Supplement_2) ◽

pp. S896-S897

Author(s):

Faris S Alnezary ◽

Tasnuva Rashid ◽

Khurshida Begum ◽

Travis J Carlson ◽

Anne J Gonzales-Luna ◽

...

Keyword(s):

Gut Microbiota ◽

Gut Microbiome ◽

Galleria Mellonella ◽

Nigella Sativa ◽

Gut Contents ◽

In Vivo Model ◽

Microbiota Composition ◽

Invertebrate Model ◽

Gut Microbiota Composition

Abstract Background Antimicrobials disrupt the gut microbiota by reducing gut microbiome diversity and quantity. Galleria mellonella provides an invertebrate model that is inexpensive, easy to maintain, and does not require specialized equipment. This study investigated the feasibility of using G. mellonella as an in vivo model to evaluate the effect of different antimicrobials on gut microbiota. Methods To determine baseline gut microbiota composition, the gut contents of G. mellonella were extracted and genomic DNA underwent shotgun meta-genomic sequencing. To determine the effect of infection and antibiotic use, 30 larvae were injected (left proleg) with ~1 × 105 colony-forming unit (cfu) of methicillin-resistant Staphylococcus aureus (MRSA) and were randomized 1:1:1 to treatment with vancomycin (20 mg/kg) or a natural antimicrobial (Nigella sativa seed oil, 70 mg/kg; NS oil), or a combination. The larvae were kept at 37°C post-infection and monitored daily for 72 hours for activity, extent of cocoon formation/growth, melanization, and survival. Two larvae from each group were randomly selected and homogenized with PBS as controls. After 24 hours of incubation, gut contents were extracted and plated for MRSA and Enterococcus cfu counts. Results Metagenomics analysis showed the gut microbiota composition of G. mellonella larvae was dominated by a subset of closely-related Enterococcus species. After 24 hours of exposure, mean Enterococcus counts were 4 × 103 cfu in the vancomycin arm and 6.2 × 104 cfu in the NS oil arm. Mean MRSA counts were 3.3 × 105 cfu in vancomycin arm and 1.5 × 104 cfu in NS oil arm. The combination of vancomycin and NS oil had higher Enterococcus counts than the vancomycin alone arm (6.3 × 104 cfu vs. 4 × 103 cfu, respectively), suggesting that NS oil may have a role in protecting the gut microbiota. Conclusion This study provides preliminary evidence to support the potential use of G. mellonella to assess the in vivo effect of a natural and synthetic antimicrobial on the gut microbiota. Disclosures All authors: No reported disclosures.

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