scholarly journals Differential Redistribution of Activated Monocyte and Dendritic Cell Subsets to the Lung Associates with Severity of COVID-19

2020 ◽  
Author(s):  
Ildefonso Sanchez-Cerrillo ◽  
Pedro Landete ◽  
Beatriz Aldave ◽  
Santiago Sanchez-Alonso ◽  
Ana Sanchez-Azofra ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Cross Sectional Study ◽  
Clinical Severity ◽  
T Cell Subsets ◽  
Viral Control ◽  
Cross Sectional ◽  
Life Threatening ◽  
Healthy Control ◽  
Classical Monocytes

The SARS-CoV-2 is responsible for the pandemic COVID-19 in infected individuals, who can either exhibit mild symptoms or progress towards a life-threatening acute respiratory distress syndrome (ARDS). It is known that exacerbated inflammation and dysregulated immune responses involving T and myeloid cells occur in COVID-19 patients with severe clinical progression. However, the differential contribution of specific subsets of dendritic cells and monocytes to ARDS is still poorly understood. In addition, the role of CD8+ T cells present in the lung of COVID-19 patients and relevant for viral control has not been characterized. With the aim to improve the knowledge in this area, we developed a cross-sectional study, in which we have studied the frequencies and activation profiles of dendritic cells and monocytes present in the blood of COVID-19 patients with different clinical severity in comparison with healthy control individuals. Furthermore, these subpopulations and their association with antiviral effector CD8+ T cell subsets were also characterized in lung infiltrates from critical COVID-19 patients. Collectively, our results suggest that inflammatory transitional and non-classical monocytes preferentially migrate from blood to lungs in patients with severe COVID-19. CD1c+ conventional dendritic cells also followed this pattern, whereas CD141+ conventional and CD123hi plasmacytoid dendritic cells were depleted from blood but were absent in the lungs. Thus, this study increases the knowledge on the pathogenesis of COVID-19 disease and could be useful for the design of therapeutic strategies to fight SARS-CoV-2 infection.

scholarly journals Urine Phenylacetylglutamine Determination in Patients with Hyperphenylalaninemia

2021 ◽  
Vol 10 (16) ◽  
pp. 3674
Author(s):  
Fernando Andrade ◽  
Ainara Cano ◽  
María Unceta Suarez ◽  
Arantza Arza ◽  
Ana Vinuesa ◽  
...  
Keyword(s):  
Potential Role ◽  
Venous Blood ◽  
Phenyl Group ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Non Invasive ◽  
Frequent Monitoring ◽  
Healthy Control

Phenylketonuria (PKU), an autosomal-recessive inborn error of phenylalanine (Phe) metabolism is the most prevalent disorder of amino acid metabolism. Currently, clinical follow-up relies on frequent monitoring of Phe levels in blood. We hypothesize that the urine level of phenylacetylglutamine (PAG), a phenyl-group marker, could be used as a non-invasive biomarker. In this cross-sectional study, a validated liquid chromatography coupled to tandem mass spectrometry (LC-MS) method was used for urinary PAG quantification in 35 participants with hyperphenylalaninemia (HPA) and 33 age- and sex-matched healthy controls. We have found that (a) PKU patients present higher urine PAG levels than healthy control subjects, and that (b) there is a significant correlation between urine PAG and circulating Phe levels in patients with HPA. In addition, we show a significant strong correlation between Phe levels from venous blood samples and from capillary finger-prick dried blood spot (DBS) samples collected at the same time in patients with HPA. Further research in order to assess the potential role of urine PAG as a non-invasive biomarker in PKU is warranted.

Suicidal Behaviors Among Adolescents – The Role of School and Home Environment

Crisis ◽  
2017 ◽  
Vol 38 (3) ◽  
pp. 168-176 ◽  
Author(s):  
Hanan El-Sayed Badr
Keyword(s):  
Suicide Ideation ◽  
Physical Violence ◽  
Cross Sectional Study ◽  
Weighting Factor ◽  
Suicidal Behaviors ◽  
Cross Sectional ◽  
School Based ◽  
Life Threatening ◽  
Mental Health Programs

Abstract. Aim: This study aimed to determine the prevalence of suicidal behaviors (ideation, planning, and attempts) among adolescents aged 13–16, and to identify psychosocial correlates of suicidal behaviors. Method: The 2010 Kuwait Global School-Based Student Health Survey (GSHS) cross-sectional study employed a two-stage cluster sample design targeting a representative sample of 2,672 students. A weighting factor was applied to make inferences to all students of the same age. Students' suicidal behaviors were the focus of this paper. Results: The prevalence rates of suicide ideation, planning, and attempts were 20.0% (95% CI = 18.5–21.6%), 14.0% (95% CI = 12.7–15.4%), and 18.1% (95% CI = 16.6–19.5%), respectively. About 26% of adolescents reported at least one suicidal behavior, while 8.5% experienced all three suicidal behaviors. Multivariate analysis revealed that girls, smoking, physical violence, feeling lonely, exposure to bullying at school, and having nonempathetic parents were significant correlates of the experience of suicidal behaviors among adolescents. Moreover, suicidal ideation stood out as a predictor of attempting suicide more than suicidal planning in both the total population and separately by gender. Conclusion: The prevalence of suicidal behaviors was alarmingly high among Kuwaiti adolescents. Adverse school and home environments strongly contributed to such behaviors. School-based mental health programs are necessary to reduce these life-threatening behaviors in Kuwait.

scholarly journals Apolipoprotein D Concentration in Human Plasma during Aging and in Parkinson’s Disease: A Cross-Sectional Study

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Andreas Waldner ◽  
Sarah Dassati ◽  
Bernhard Redl ◽  
Nicola Smania ◽  
Marialuisa Gandolfi
Keyword(s):  
Healthy Subjects ◽  
Neurological Impairment ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Age Related ◽  
Healthy Control ◽  
Apolipoprotein D ◽  
The Relationship ◽  
Small Hydrophobic

Apolipoprotein D (ApoD), a lipocalin transporter of small hydrophobic molecules, plays an important role in several neurodegenerative diseases. ApoD is expressed in and secreted from a variety of peripheral and brain tissues. Increments of ApoD have been reported in relation with oxidative stress conditions, aging, and degeneration in the nervous system. Preliminary findings support the role of ApoD in neuroprotection. However, its role in PD remains unclear. To date, no studies have been performed on the relationship between ApoD in the blood and PD, as neurodegenerative pathology related to oxidative damage. We investigated the concentration of ApoD in the blood of healthy control subjects and PD patients with mild-to-moderate neurological impairment. ApoD plasma levels were measured using sandwich enzyme-linked immunosorbent assays (ELISA) in 90 healthy subjects (aging-analysis cohort) and in 66 PD patients at different stages compared with 19 age-matched healthy subjects. Significant age-related increase of ApoD was detected in subjects older than 65 years of age (p<0.002). In PD patients, a significant increase in ApoD plasma concentration was found compared with healthy subjects of the same age (p<0.05). ApoD and PD stage are significantly correlated (p<0.05). ApoD might be a valid marker for the progression of PD.

scholarly journals The Role of Angiopoietin-2 Gene Mutation on Clinical Severity of Dengue Disease in Children

2020 ◽  
Vol 8 (B) ◽  
pp. 1110-1115
Author(s):  
Mayetti Akmal ◽  
Amirah Zatil Izzah ◽  
Jamsari ◽  
Eriyati Darwin ◽  
Dadang Hudaya Somasetia
Keyword(s):  
Dengue Shock Syndrome ◽  
Cross Sectional Study ◽  
Clinical Severity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chain Reaction ◽  
Cross Sectional ◽  
Dengue Disease ◽  
Polymerase Chain ◽  
Angiopoietin 2

BACKGROUND: In general, angiopoietin-2 levels are increased concomitantly with dengue clinical severity. AIM: This research aims to determine the role of mutation on angiopoietin-2 on dengue clinical severity. METHODS: A cross-sectional study of 108 children with dengue disease grouped by severity. Angiopoietin-2 level was examined by enzyme-linked immunosorbent assay. Polymerase chain reaction and double nucleic acid sequencing are using 2 Exon 4-F primers. RESULTS: Angiopoietin-2 levels on rs7834131 mutant are higher in dengue fever (p < 0.05) and dengue hemorrhage fever group than non-mutant, while on dengue shock syndrome, it is lower than non-mutant. CONCLUSION: Angiopoietin-2 mutation on rs7834131 might have a protective effect on dengue disease severity.

Role of Sildenafil in the Treatment of Persistent Pulmonary Hypertension of Newborn (PPHN) - Should it Be Withdrawn from Market of Bangladesh due to Threat of being Misuses

2020 ◽  
Vol 35 (2) ◽  
pp. 100-104
Author(s):  
Maksudur Rahman ◽  
Mohammad Abdullah Al Mamun ◽  
MAK Azad Chowdhury ◽  
Abu Sayeed Munsi
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Neonatal Intensive Care ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cardiac Intensive Care Unit ◽  
Cross Sectional ◽  
Cardiac Intensive Care ◽  
Hospital Protocol

Background: Recently it has been apprehended that sildenafil, a drug which has been successfully using in the treatment of PPHN and erectile dysfunction in adult, is going to be withdrawn from the market of Bangladesh due to threat of its misuses. Objective: The aim of this study was to see the extent of uses of sildenafil in the treatment of PPHN and importance of availability of this drugs in the market inspite of its probable misuses. Methods: This cross sectional study was conducted in neonatal intensive care unit (NICU), special baby care unit (SCABU) and cardiac intensive care unit (CICU) of Dhaka Shishu (Children) Hospital from June, 2017 to May 2018. Neonates with PPHN were enrolled in the study. All cases were treated with oral sildenefil for PPHN along with others management according to hospital protocol. Data along with other parameters were collected and analyzed. Results: Total 320 patients with suspected PPHN were admitted during the study period. Among them 92 (29%) cases had PPHN. Male were 49(53 %) cases and female were 43(47%) cases. Mean age at hospital admission was 29.7±13.4 hours. Based on echocardiography,13(14%) cases had mild, 38 (41%) cases moderate and 41(45%) cases severe PPHN. Mean duration of sildenafil therapy was 11.9±7.1 days. Improved from PPHN were 83 (90%) cases. Mortality was 10% (9). Conclusion: In this study it was found that the incidence of PPHN is 29% among the suspected newborns. Sildenafil is successfull in improving the oxygenation of PPHN and to decrease the mortality of neonates. DS (Child) H J 2019; 35(2) : 100-104

Role of Fibrinolytic Therapy in Empyema Thoracis – A Hospital Based one year Cross Sectional Study

2012 ◽  
Vol 2 (2) ◽  
pp. 345-350
Author(s):  
Dr. Girish.L Dandagi ◽  
◽  
Venkat kalyana kumar. P ◽  
Dr. Dr.Isaac Mathew ◽  
Dr. Dr.G S Gaude Dr. Dr.G S Gaude
Keyword(s):  
Cross Sectional Study ◽  
Fibrinolytic Therapy ◽  
Empyema Thoracis ◽  
Sectional Study ◽  
Cross Sectional ◽  
One Year

Breastfeeding practices among mothers of infants in Peringara Panchayat in Pathanamthitta District

2018 ◽  
Vol 3 (01) ◽  
Author(s):  
Aswathy S. ◽  
Lakshmi M. K.
Keyword(s):  
Exclusive Breastfeeding ◽  
Cross Sectional Study ◽  
Community Based ◽  
Chi Square ◽  
Cross Sectional ◽  
Breastfeeding Practices ◽  
Chi Square Test ◽  
Type Of Delivery ◽  
Initiation Of Breastfeeding

The study was aimed to assess the breastfeeding practices among mothers of infants in Peringara Gramapanchayat in Kerala. Study was a community based cross-sectional study among mothers of infants in Peringara gramapanchayat using a pretested questionnaire. 142 breastfeeding mothers of infants in Peringara gramapanchayat were studied and mothers who were not present at home during the study were excluded from the study. Study period consisted of 18 days between December 2015 and January 2016. Study variables includes type of delivery, initiation of breastfeeding, breastfeeding practices and role of ASHAs in promoting good breastfeeding practices. Statistical analysis was done using Pearson’s Chi-square test and T test. The study found that exclusive breastfeeding has been done by 68.3% of mothers. There is no practice of giving pre-lacteal feed, 95.8% of mothers have given colostrum to the new born. Statistically significant association was found between the type of delivery and time of initiation of breastfeeding (p less than 0.05). Time of initiation of breastfeeding was prolonged in case of Caesarean section. 49.3% of mothers have breastfed the baby within one hour. 55.6% of mothers were informed about importance of breastfeeding by ASHAs and only 20.4% of mothers were informed about period of exclusive breastfeeding and period of complimentary feeding by ASHAs.

Learning Electrocardiogram Interpretation: Insights from Residents and a Proposed Solution in an Observational Study (Preprint)

2020 ◽  
Author(s):  
Mohammad Alakchar ◽  
Abdisamad M. Ibrahim ◽  
Mohsin Salih ◽  
Mukul Bhattarai ◽  
Nitin Tandan ◽  
...  
Keyword(s):  
Internal Medicine ◽  
Correlation Coefficient ◽  
Online Survey ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Resident Attitudes ◽  
Internal Medicine Residents ◽  
Life Threatening ◽  
Degree Of Participation ◽  
Electrocardiogram Interpretation

BACKGROUND Interpretation of electrocardiograms (EKG) is an essential tool for every physician. Despite this, the diagnosis of life-threatening pathology on EKG remains suboptimal in trainees. The purpose of this study is to study resident attitudes and behaviours towards EKGs, and describe an innovative way to teach EKGs. OBJECTIVE Study attitudes and behaviours towards EKGs. Describe an innovative way to teach EKGs. METHODS Design: An observational cross-sectional study through an anonymous online survey of resident attitudes and comfort with EKG interpretation. This was followed by creation of a WhatsApp group for discussion and interpretation of EKGs with peers. At the end of the day, the official EKG interpretation was posted. Setting: Internal medicine residency at Southern Illinois University. Participants: Internal medicine residents Interventions: Creation of WhatsApp group to aid with EKG interpretation Measurements: A 17 item questionnaire, followed by detection of degree of participation in a WhatsApp group. RESULTS Forty-one out of 63 residents (65%) completed the survey. 85% of respondents thought that an interactive way to teach EKGs is the best method of teaching, and 73% did not feel confident interpreting EKGs. 30% often rely on automated EKG interpretation. Further analysis indicated that PGY-1 residents reported ordering fewer EKGs (correlation coefficient -0.399, p = 0.012) and were uncomfortable diagnosing QT prolongation on an EKG (correlation coefficient -0.310, p = 0.049). Residents in the third or greater year of training ordered more EKGs (correlation coefficient 0.379, p = 0.015), less frequently relied on the computer for EKG diagnosis (correlation coefficient 0.399, p = 0.010), and were comfortable diagnosing an acute myocardial infarction and atrial arrhythmias. CONCLUSIONS In conclusion, most IM trainees do not feel comfortable interpreting EKG, however, this does improve with PGY year. WhatsApp is a possible platform for teaching EKGs.

scholarly journals Factors associated with emotional exhaustion in healthcare professionals involved in the COVID-19 pandemic: an application of the job demands-resources model

2021 ◽  
Author(s):  
Serena Barello ◽  
Rosario Caruso ◽  
Lorenzo Palamenghi ◽  
Tiziana Nania ◽  
Federica Dellafiore ◽  
...  
Keyword(s):  
Emotional Exhaustion ◽  
Patient Engagement ◽  
Healthcare Professionals ◽  
Job Demands ◽  
Cross Sectional Study ◽  
Personal Resources ◽  
Sectional Study ◽  
Cross Sectional ◽  
The Impact

Abstract Purpose The purpose of the present cross-sectional study is to investigate the role of perceived COVID-19-related organizational demands and threats in predicting emotional exhaustion, and the role of organizational support in reducing the negative influence of perceived COVID-19 work-related stressors on burnout. Moreover, the present study aims to add to the understanding of the role of personal resources in the Job Demands-Resources model (JD-R) by examining whether personal resources—such as the professionals’ orientation towards patient engagement—may also strengthen the impact of job resources and mitigate the impact of job demands. Methods This cross-sectional study involved 532 healthcare professionals working during the COVID-19 pandemic in Italy. It adopted the Job-Demands-Resource Model to study the determinants of professional’s burnout. An integrative model describing how increasing job demands experienced by this specific population are related to burnout and in particular to emotional exhaustion symptoms was developed. Results The results of the logistic regression models provided strong support for the proposed model, as both Job Demands and Resources are significant predictors (OR = 2.359 and 0.563 respectively, with p < 0.001). Moreover, healthcare professionals’ orientation towards patient engagement appears as a significant moderator of this relationship, as it reduces Demands’ effect (OR = 1.188) and increases Resources’ effect (OR = 0.501). Conclusions These findings integrate previous findings on the JD-R Model and suggest the relevance of personal resources and of relational factors in affecting professionals’ experience of burnout.

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