scholarly journals Nested PCR for the detection of Taenia solium DNA in stool samples

2020 ◽  
Author(s):  
C Franco-Muñoz ◽  
A Arévalo ◽  
S Duque-Beltran
Keyword(s):  
Detection Limit ◽  
Nested Pcr ◽  
Intestinal Parasites ◽  
Taenia Solium ◽  
Cross Reaction ◽  
Public Health Importance ◽  
Alkaline Lysis ◽  
Pcr Products ◽  
Stool Samples ◽  
Dna Pcr

ABSTRACTThe traditional parasitological method to diagnose taeniasis is the microscopic observation of eggs in stool samples. However, this method does not allow differentiation between Taenia saginata and Taenia solium. This aim of this study was to achieve the detection of T. solium DNA by polymerase chain reaction (PCR) and to evaluate the cross-reaction with other species of the genus Taenia and other intestinal parasites. DNA was extracted from adult T. solium cestodes by cryolysis in liquid nitrogen and with the DNA stool extraction kit from Qiagen. The detection limit of the test was evaluated by DNA dilutions in water and in stool samples. DNA was extracted from proglottids of T. saginata and T. crassiceps and from stool samples containing other intestinal parasites using ethanol treatment, alkaline lysis, and the DNA stool extraction kit. Nested PCR was used to amplify a previously described fragment of the Tso31 gene, and the PCR products were analyzed by electrophoresis in 2% agarose gels followed by staining with GelRed. The nested PCR of the Tso31 gene allowed the detection of T. solium DNA in stool samples with a detection limit of 20 pg of parasite DNA. PCR showed no cross-reaction with T. saginata, T. crassiceps, or other intestinal parasites of public health importance in Colombia.

Prevalence of intestinal parasites and associated factors among children from child centres in Bahir Dar city, northwest Ethiopia

2020 ◽  
Vol 50 (3) ◽  
pp. 194-198
Author(s):  
Aynework Abebaw ◽  
Getaneh Alemu ◽  
Animen Ayehu
Keyword(s):  
Intestinal Parasites ◽  
Parasitic Infection ◽  
Single Species ◽  
Cross Sectional Study ◽  
Northwest Ethiopia ◽  
Bahir Dar ◽  
Public Health Importance ◽  
Cross Sectional ◽  
Control Programmes ◽  
Stool Samples

Data concerning the burden of parasites among populations living in overcrowded circumstances have paramount importance for the success of interventions aimed at eliminating such disease. A cross-sectional study was therefore conducted from February to May 2019 among 217 children at child centres in Bahir Dar city. Data were collected using a structured questionnaire and stool samples were processed for parasitological investigation. A total of 53 (24.4%) children were infected with at least a single species of intestinal parasite. Giardia lamblia (7.8%) was the most frequent, followed by Hymenolepis nana (6%). Children with untrimmed fingernails (adjusted odds ratio [AOR] = 2.422, P = 0.027) were significantly more prone to infection. Similarly, children with no habit of hand washing after soil contact (AOR = 2.752, P = 0.014) or after defecation (AOR = 3.087, P = 0.026) were significantly associated with parasitic infection. The prevalence of intestinal parasites at child centres in Bahir Dar is of public health importance; therefore, control programmes should target these children.

INTESTINAL PARASITE INFECTION IN HUE UNIVERSITY HOSPITAL IN 2013-2014 BY DIRECT EXAMINATION AND IMMUNOLOGICAL DIAGNOSTICS

2016 ◽  
pp. 105-111
Author(s):  
Nu Phuong Anh Ton ◽  
Thi Minh Chau Ngo
Keyword(s):  
Intestinal Parasite ◽  
Intestinal Parasites ◽  
Clonorchis Sinensis ◽  
Taenia Solium ◽  
University Hospital ◽  
High Rate ◽  
Intestinal Helminth ◽  
Direct Examination ◽  
Life Condition ◽  
Parasite Infections

Introduction: Intestinalparasite infections still are very common in tropical country such as Vietnam. Therefore evaluation of the prevalence of them should be done. Materials and methods: A crosssectional descriptivestudy were carried out to use the wet mount direct examination, Kato and ELISA technique for evaluation the rate of intestinal parasite infections, interviewed them to reveal their life’s hygenic condition, knowledge and risk behaviour of intestinal parasite infections in 640 patients attending to the in Hue University of hospital. Result: The rate of soiltransmitted intestinal helminth were lower than the foodborne infection of cestode and trematode such as: Taenia solium/saginata 3.64%, Clonorchis sinensis 2.73%. The rate of positive antibody of rare intestinal parasite were Fasciola gigantica 40.68%, E. histolytica/dispar 43.75%, Toxocara canis 32.43%, Gnasthostoma spinigerum 37.5%, Taenia solium 51.43%, Strongyloidesstercoralis 16.67%. Most of people has hygenic life condition with hygenic water available. The popular knowledge of intestinal parasite diseases of transmission, pathology, prevention and treatment were concentrate on oiltransmittedintestinal helminth.There were high rate of platyhelminths infectious behaviourssuch as: eating raw water plant 75.6%, eating raw beef 80.6%, eating raw pork 72.2%, eating raw fish 11.8%, bare foot 34.2%. Conclusions: The result of our study showed that the higher rate of foodborne cestode and trematode than soiltransmittedhelminth correspond tothe hygenic life condition, the knowledge of the diseases and risk behavior of intestinal parasites infection. Key words: Helminth, cestode, trematode

scholarly journals Parasitic infections in Swiss children: Are we overtesting?

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Corinne Légeret ◽  
Céline Rüttimann ◽  
Hans Fankhauser ◽  
Henrik Köhler
Keyword(s):  
Retrospective Study ◽  
Positive Result ◽  
Pediatric Patients ◽  
Intestinal Parasites ◽  
Gastrointestinal Symptoms ◽  
International Travel ◽  
Parasitic Infections ◽  
Stool Samples ◽  
Stool Specimens

Abstract Background A wide variation of causes can lead to gastrointestinal symptoms in children- an infection with parasites is one of them. The expansion of international travel might lead to an increase in testing children for a correspondent infection. Currently there are no guidelines available, which patients should be tested for a possible parasitical infection. The aim of the study was to characterize Swiss children suffering from intestinal parasites, in order to provide more knowledge for the clinician who should be tested. Methods This is a retrospective study of Swiss pediatric patients, whose stools have been tested for parasites and helminths. Results A total of 1855 stool samples, belonging to 572 different children with an average age of 7.9 years, were tested within a 10-year period. The prevalence of a positive result was 4.2%, of which all were positive for Blastocystis, and 12.5% had a co-infection with Endolimax nana. Conclusion Immigrants, immune compromised children with diarrhea and pediatric patients with bloody or protracted diarrhea should have 2 different stool specimens examined for a possible parasitical infection.

scholarly journals Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria

2020 ◽  
Vol 9 (1) ◽  
pp. 54
Author(s):  
Salem Belkessa ◽  
Daniel Thomas-Lopez ◽  
Karim Houali ◽  
Farida Ghalmi ◽  
Christen Rune Stensvold
Keyword(s):  
Real Time ◽  
Molecular Characterization ◽  
Real Time Pcr ◽  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Specific Pcr ◽  
Stool Samples ◽  
Pcr Targeting ◽  
Assemblage B

The molecular epidemiology of giardiasis in Africa remains unclear. A study was carried out across four hospitals in Algeria. A total of 119 fecal samples from 55 children, 37 adults, and 27 individuals of undetermined age, all scored positive for intestinal parasites by microscopy, and were screened by real-time PCR for Giardia. Molecular characterization of Giardia was performed by assemblage-specific PCR and PCR targeting the triose phosphate isomerase gene (tpi). Of the 119 samples, 80 (67%) were Giardia-positive by real-time PCR. For 48 moderately-highly real-time PCR-positive samples, tpi genotyping assigned 22 samples to Assemblage A and 26 to Assemblage B. Contrary to Assemblage A, Assemblage B exhibited substantial genetic diversity and allelic heterozygosity. Assemblage-specific PCR proved to be specific for discriminating Assemblage A or B but not as sensitive as tpi genotyping. We confirmed that real-time PCR is more sensitive than microscopy for detecting Giardia in stool samples and that robust amplification and sequencing of the tpi gene is feasible when moderate-to-strongly real-time PCR-positive samples are used. This study is one of the few performed in Africa providing genotyping data on Giardia infections in humans. Both assemblages A and B were commonly seen and not associated with specific sociodemographic data.

scholarly journals Prevalence and Correlates of Intestinal Parasites among Patients Admitted to Mirembe National Mental Health Hospital, Dodoma, Tanzania

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Azan A. Nyundo ◽  
David Z. Munisi ◽  
Ainory P. Gesase
Keyword(s):  
Neglected Tropical Diseases ◽  
Intestinal Parasites ◽  
Psychiatric Patients ◽  
Parasitic Infection ◽  
Parasitic Infections ◽  
Intestinal Parasitic Infection ◽  
Psychiatric Facility ◽  
Concentration Data ◽  
Cross Sectional ◽  
Stool Samples

Background. Neglected tropical diseases continue to be one of the leading causes of morbidity and mortality in the developing world. Psychiatric patients are among groups at risk for parasitic infection although control and monitoring programs largely overlook this population. This study aimed at determining prevalence and factors associated with intestinal parasitic infection among patients admitted to a psychiatric facility.Method. The study followed cross-sectional design; all the residing patients that met the inclusion criteria were included in the survey. Stool samples were collected and examined by direct wet preparation and formol-ether concentration. Data were analyzed with STATA version 12.1; Chi-square test was computed to determine the level of significance atpvalue < 0.05.Results.Of all 233 patients who returned the stool samples, 29 (12.45%) screened were positive for an intestinal parasite. There was no significant association between parasite carriage and age, sex, or duration of hospital stay.Conclusion. The study shows that intestinal parasitic infection is common among patients in a psychiatric facility and highlights that parasitic infections that enter through skin penetration may be a more common mode of transmission than the oral route. Furthermore, the study underscores the need for surveillance and intervention programs to control and manage these infections.

scholarly journals Do intestinal parasites interfere with the seroepidemiologic surveillance of Schistosoma mansoni infection?

1996 ◽  
Vol 116 (3) ◽  
pp. 323-329 ◽  
Author(s):  
B. Alarcón De Noya ◽  
C. Colmenares ◽  
S. Losada ◽  
Z. Fermin ◽  
G. Masroua ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
False Positive ◽  
Total Population ◽  
Intestinal Parasites ◽  
Field Work ◽  
Cross Reactivity ◽  
Cross Reaction ◽  
Protein Fractions ◽  
Egg Antigen ◽  
Immunoenzymatic Assay

SUMMARYIn view of the known cross-reactivity of sera from patients with intestinal parasites to some Schistosoma mansoni antigens, field work was conducted in an area of Venezuela non-endemic for schistosomiasis using the routine immunoenzymatic assay (ELISA) with soluble egg antigen (SEA). False positive reactions represented 15·3% of the total population as determined by SEA–ELISA. SEA-immunoblotting of the false positive sera indicated that protein fractions of 91 and 80 kDa appear to be responsible for cross-reactivity. Sera from hookworm infected individuals produced a higher frequency and intensity of cross-reaction than other sera. SEA-fractions of 105, 54, 46, 42, 32, 25 and 15 kDa were the most specific.

scholarly journals Detection of Cryptosporidium spp and other intestinal parasites in children with acute diarrhea and severe dehydration in Rio de Janeiro

2007 ◽  
Vol 40 (3) ◽  
pp. 346-348 ◽  
Author(s):  
Filipe Anibal Carvalho-Costa ◽  
Alessandra Queiroga Gonçalves ◽  
Sandra Laranjeira Lassance ◽  
Carla Pontes de Albuquerque ◽  
José Paulo Gagliardi Leite ◽  
...  
Keyword(s):  
Giardia Lamblia ◽  
Intestinal Parasites ◽  
Blue Staining ◽  
Pediatric Hospital ◽  
Severe Dehydration ◽  
Enterobius Vermicularis ◽  
Blastocystis Hominis ◽  
Entamoeba Dispar ◽  
Cryptosporidium Spp ◽  
Stool Samples

The objective of the present study was to estimate the frequency of infection by Cryptosporidium spp and other intestinal parasites in dehydrated children with gastroenteritis who were admitted to a pediatric hospital. Stool examinations from 218 children were performed. Cryptosporidium spp was identified in eighteen out of 193 stool samples (9.3%) subjected to safranin-methylene blue staining. Giardia lamblia was detected in ten out of 213 (4.7%) samples examined via the direct or Ritchie methods. Other parasites identified were Ascaris lumbricoides (4.2%), Blastocystis hominis (1.4%), Entamoeba coli (0.9%), Entamoeba histolytica/Entamoeba dispar (0.5%), Endolimax nana (0.5%), Trichuris trichiura (0.5%) and Enterobius vermicularis (0.5%).

scholarly journals First Report of a Group 16SrII Phytoplasma Infecting Chickpea in Oman

Plant Disease ◽  
2006 ◽  
Vol 90 (7) ◽  
pp. 973-973 ◽  
Author(s):  
N. A. Al-Saady ◽  
A. M. Al-Subhi ◽  
A. Al-Nabhani ◽  
A. J. Khan
Keyword(s):  
Nested Pcr ◽  
Animal Feed ◽  
Restriction Enzymes ◽  
Universal Primers ◽  
Polymorphism Analysis ◽  
Disease Symptoms ◽  
First Report ◽  
Pcr Products ◽  
Polymerase Chain ◽  
Group 2

Chickpea (Cicer arietinum), locally known as “Dungo”, is grown for legume and animal feed mainly in the interior region of Oman. During February 2006, survey samples of chickpea leaves from plants showing yellows disease symptoms that included phyllody and little leaf were collected from the Nizwa Region (175 km south of Muscat). Total nucleic acid was extracted from asymptomatic and symptomatic chickpea leaves using a cetyltrimethylammoniumbromide method with modifications (3). All leaf samples from eight symptomatic plants consistently tested positive using a polymerase chain reaction assay (PCR) with phytoplasma universal primers (P1/P7) that amplify a 1.8-kb phytoplasma rDNA product and followed by nested PCR with R16F2n/R16R2 primers yielding a product of 1.2 kb (2). No PCR products were evident when DNA extracted from healthy plants was used as template. Restriction fragment length polymorphism analysis of nested PCR products by separate digestion with Tru9I, HaeIII, HpaII, AluI, TaqI, HhaI, and RsaI restriction enzymes revealed that a phytoplasma belonging to group 16SrII peanut witches'-broom group (2) was associated with chickpea phyllody and little leaf disease in Oman. Restriction profiles of chickpea phytoplasma were identical with those of alfalfa witches'-broom phytoplasma, a known subgroup 16SrII-B strain (3). To our knowledge, this is the first report of phytoplasma infecting chickpea crops in Oman. References: (1) A. J. Khan et al. Phytopathology, 92:1038, 2002. (2). I.-M. Lee et al. Int. J. Syst. Bacteriol. 48:1153, 1998 (3) M. A. Saghai-Maroof et al. Proc. Natl. Acad. Sci. USA. 81:8014, 1984.

scholarly journals Domiciliary Cockroaches as Carriers of Human Intestinal Parasites in Lagos Metropolis, Southwest Nigeria: Implications for Public Health

2018 ◽  
pp. 141-151 ◽  
Author(s):  
Adedotun A Adenusi ◽  
Mary I Akinyemi ◽  
Dele Akinsanya
Keyword(s):  
Public Health ◽  
Disease Transmission ◽  
Periplaneta Americana ◽  
Intestinal Parasites ◽  
Low Cost ◽  
Parasite Prevalence ◽  
Strongyloides Stercoralis ◽  
Housing Type ◽  
Public Health Importance ◽  
Southwest Nigeria

Background: Domiciliary cockroaches are obnoxious pests of significant medical importance. We investigated the prevalence of human intestinal parasites in cockroaches and its attendant public health importance. Methods: Overall, 749 cockroaches (Periplaneta americana, 509, Blattella germanica, 240) caught by trapping from 120 households comprising 3 different housing types in Somolu, Lagos metropolis, southwest Nigeria, in 2015 were screened for human intestinal parasites using standard parasitological techniques. Results: The prevalence of human intestinal parasites in cockroaches was 96.4%. There was no statistically signifi­cant difference (P> 0.05) in parasite prevalences between P. americana (95.7%) and B. germanica (97.9%). Parasite species identified and their prevalence were as follows: Entamoeba histolytica/dispar (44.1%), E. coli (37.8%), Gi­ardia lamblia (18.7%), Cryptosporidium sp. (13.8%), Ascaris lumbricoides (61.3%), Trichuris trichiura (55.8%), hookworms (11.6%), Strongyloides stercoralis (11.7%), Taenia/Echinococcus spp. (10.5%), Enterobius vermicularis (17.2%) and Hymenolepis nana (11.6%). Parasite prevalence and burdens varied with housing type; the prevalence was significantly higher statistically (P< 0.05) in cockroaches from low-cost bungalow, LCB (100%) and low-cost, 2-storey, LC2-S (100%) houses than in medium-cost flats, MCF (81.3%). Parasite burdens were also significantly higher statistically (P< 0.05) in cockroaches from LCB or LC2-S than in cockroaches from MCF. Parasite preva­lences between cockroach gut and body surfaces were not statistically significant (P> 0.05) but mean parasite bur­dens in gut were significantly higher statistically (P< 0.05) than on body surfaces. Conclusion: Cockroaches types carry transmissive stages of human intestinal parasites and may act as reservoirs and potential mechanical vectors for disease transmission.

Sign in / Sign up

Export Citation Format

Share Document