scholarly journals Surveillance of genetic diversity and evolution in locally transmitted SARS-CoV-2 in Pakistan during the first wave of the COVID-19 pandemic

2021 ◽  
Author(s):  
Muhammad Shakeel ◽  
Muhammad Irfan ◽  
Zaibunnisa ◽  
Muhammad Rashid ◽  
Sabeeta Kanwal Ansari ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Hot Spot ◽  
Spike Protein ◽  
Independent Events ◽  
Recurrent Mutations ◽  
The Usa ◽  
Spatio Temporal ◽  
B Lineage ◽  
The Uk ◽  
The City

AbstractSurveillance of genetic diversity in the SARS-CoV-2 is extremely important to detect the emergence of more infectious and deadly strains of the virus. In this study, we monitored mutational events in the SARS-CoV-2 genome through whole genome sequencing. The samples (n=48) were collected from the hot spot regions of the metropolitan city Karachi, Pakistan during the four months (May 2020 to August 2020) of first wave of the COVID-19 pandemic. The data analysis highlighted 122 mutations, including 120 single nucleotide variations (SNV), and 2 deletions. Among the 122 mutations, there were 71 singletons, and 51 recurrent mutations. A total of 16 mutations, including 5 nonsynonymous mutations, were detected in spike protein. Notably, the spike protein missense mutation D614G was observed in 31 genomes. The phylogenetic analysis revealed majority of the genomes (36) classified as B lineage, where 2 genomes were from B.6 lineage, 5 genomes from B.1 ancestral lineage and remaining from B.1 sub-lineages. It was noteworthy that three clusters of B.1 sub-lineages were observed, including B.1.36 lineage (10 genomes), B.1.160 lineage (11 genomes), and B.1.255 lineage (5 genomes), which represent independent events of SARS-CoV-2 transmission within the city. The sub-lineage B.1.36 had higher representation from the Asian countries and the UK, B.1.160 correspond to the European countries with highest representation from the UK, Denmark, and lesser representation from India, Saudi Arabia, France and Switzerland, and the third sub-lineage (B.1.255) correspond to the USA. Collectively, our study provides meaningful insight into the evolution of SARS-CoV-2 lineages in spatio-temporal local transmission during the first wave of the pandemic.

scholarly journals Molecular Epidemiology Surveillance of SARS-CoV-2: Mutations and Genetic Diversity One Year after Emerging

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 184
Author(s):  
Alejandro Flores-Alanis ◽  
Armando Cruz-Rangel ◽  
Flor Rodríguez-Gómez ◽  
James González ◽  
Carlos Alberto Torres-Guerrero ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Spike Protein ◽  
The Novel ◽  
Molecular Surveillance ◽  
Low Genetic Diversity ◽  
Geographical Regions ◽  
Frequent Mutations ◽  
One Year ◽  
The City ◽  
Over Time

In December 2019, the first cases of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were identified in the city of Wuhan, China. Since then, it has spread worldwide with new mutations being reported. The aim of the present study was to monitor the changes in genetic diversity and track non-synonymous substitutions (dN) that could be implicated in the fitness of SARS-CoV-2 and its spread in different regions between December 2019 and November 2020. We analyzed 2213 complete genomes from six geographical regions worldwide, which were downloaded from GenBank and GISAID databases. Although SARS-CoV-2 presented low genetic diversity, there has been an increase over time, with the presence of several hotspot mutations throughout its genome. We identified seven frequent mutations that resulted in dN substitutions. Two of them, C14408T>P323L and A23403G>D614G, located in the nsp12 and Spike protein, respectively, emerged early in the pandemic and showed a considerable increase in frequency over time. Two other mutations, A1163T>I120F in nsp2 and G22992A>S477N in the Spike protein, emerged recently and have spread in Oceania and Europe. There were associations of P323L, D614G, R203K and G204R substitutions with disease severity. Continuous molecular surveillance of SARS-CoV-2 will be necessary to detect and describe the transmission dynamics of new variants of the virus with clinical relevance. This information is important to improve programs to control the virus.

scholarly journals Growing public spaces in the city: Community gardening and the making of new urban environments of publicness

Urban Studies ◽  
2021 ◽  
pp. 004209802097228
Author(s):  
Paul Milbourne
Keyword(s):  
Public Space ◽  
Public Spaces ◽  
Urban Environments ◽  
Community Gardening ◽  
City Community ◽  
Space Transformation ◽  
The Usa ◽  
The Uk ◽  
Dominant Narratives ◽  
The City

The demise of public space in cities across the Global North has received considerable scrutiny from urban scholars in recent years, with accounts of the loss, privatisation and increased regulation of public space prevalent within the academic literature. This paper seeks to complicate these dominant narratives of public space transformation by exploring the complexities of existing public spaces and the emergence of new spaces of publicness in the city. It uses a case study of community gardening in mundane and everyday neighbourhood spaces to provide a more nuanced and progressive reading of the relations between publicness and space in the city. Drawing on empirical materials from recent research on community gardening projects in 15 cities in Australia, Canada, the UK and the USA, the paper highlights how community gardening is creating new environments of publicness across public, private and in-between spaces that complicate both the end of public space discourse and conventional understandings of public space within urban studies.

scholarly journals Mechanisms for spatio-temporal pattern formation in highway traffic models

2008 ◽  
Vol 366 (1872) ◽  
pp. 2017-2032 ◽  
Author(s):  
R. Eddie Wilson
Keyword(s):  
Pattern Formation ◽  
Trajectory Data ◽  
Highway Traffic ◽  
Traffic Models ◽  
Traffic Jam ◽  
Traffic Pattern ◽  
Stop And Go ◽  
The Usa ◽  
Spatio Temporal ◽  
The Uk

A key qualitative requirement for highway traffic models is the ability to replicate a type of traffic jam popularly referred to as a phantom jam , shock wave or stop-and-go wave . Despite over 50 years of modelling, the precise mechanisms for the generation and propagation of stop-and-go waves and the associated spatio-temporal patterns are in dispute. However, the increasing availability of empirical datasets, such as those collected from motorway incident detection and automatic signalling system (MIDAS) inductance loops in the UK or the next-generation simulation trajectory data (NGSIM) project in the USA, means that we can expect to resolve these questions definitively in the next few years. This paper will survey the essence of the competing explanations of highway traffic pattern formation and introduce and analyse a new mechanism, based on dynamical systems theory and bistability, which can help resolve the conflict.

scholarly journals Stability of Patches of Higher Population Density within the Heterogenous Distribution of the Gray Field Slug Deroceras reticulatum in Arable Fields in the UK

Insects ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 9
Author(s):  
Emily Forbes ◽  
Matthew Back ◽  
Andrew Brooks ◽  
Natalia B. Petrovskaya ◽  
Sergei V. Petrovskii ◽  
...  
Keyword(s):  
Linear Models ◽  
Hot Spot ◽  
Temporal Stability ◽  
Soil Surface ◽  
Deroceras Reticulatum ◽  
Arable Fields ◽  
Regular Sampling ◽  
Spatio Temporal ◽  
Adverse Environmental Conditions ◽  
The Uk

Exploitation of heterogenous distributions of Deroceras reticulatum, in arable fields by targeting molluscicide applications toward areas with higher slug densities, relies on these patches displaying sufficient spatio-temporal stability. Regular sampling of slug activity/distribution was undertaken using 1 ha rectangular grids of 100 refuge traps established in 22 commercial arable field crops. Activity varied significantly between the three years of the study, and the degree of aggregation (Taylor’s Power Law) was higher in fields with higher mean trap catches. Hot spot analysis detected statistically significant spatial clusters in all fields, and in 162 of the 167 individual assessment visits. The five assessment visits in which no clusters were detected coincided with low slug activity (≤0.07 per trap). Generalized Linear Models showed significant spatial stability of patches in 11 fields, with non-significant fields also characterized by low slug activity (≤1.2 per trap). Mantel’s permutation tests revealed a high degree of correlation between location of individual patches between sampling dates. It was concluded that patches of higher slug density were spatio-temporally stable, but detection using surface refuge traps (which rely on slug activity on the soil surface) was less reliable when adverse environmental conditions resulted in slugs retreating into the upper soil horizons.

Bible on the territory of Ukraine

1998 ◽  
pp. 41-42
Author(s):  
V. Jukovskyy
Keyword(s):  
The Bible ◽  
Christian Morality ◽  
The Usa ◽  
Younger Generation ◽  
The City ◽  
Practical Conference

On June 5-7, 1998, in the city of Ostroh, Rivne Oblast, on the basis of the Ostroh Academy, the IV International Scientific and Practical Conference "Educating the Younger Generation on the Principles of Christian Morality in the Process of the Spiritual Revival of Ukraine" was held. This year she was devoted to the topic "The Bible on the Territory of Ukraine". About 400 philosophers, psychologists and educators from many Ukrainian cities, as well as philosophers and educators from Belarus, Canada, Poland, Russia, the USA, Turkey and Sweden participated in her work. The conference was attended by theologians and priests of all Christian denominations of Ukraine.

An early mainstream economist from Hungary: István Varga (1897–1962)

2003 ◽  
Vol 53 (2) ◽  
pp. 195-213 ◽  
Author(s):  
K. Majoros
Keyword(s):  
Austrian Economic ◽  
National Income ◽  
Anglo Saxon ◽  
Turbulent Times ◽  
The Usa ◽  
New Ideas ◽  
Modern Economic ◽  
The Uk ◽  
Economic Thinking ◽  
Lasting Influence

The study introduces a Hungarian economic thinker, István Varga*, whose valuable activity has remained unexplored up to now. He became an economic thinker during the 1920s, in a country that had not long before become independent of Austria. The role played by Austria in the modern economic thinking of that time was a form of competition with the thought adhered to by the UK and the USA. Hungarian economists mainly interpreted and commented on German and Austrian theories, reasons for this being that, for example, the majority of Hungarian economists had studied at German and Austrian universities, while at Hungarian universities principally German and Austrian economic theories were taught. István Varga was familiar not only with contemporary German economics but with the new ideas of Anglo-Saxon economics as well — and he introduced these ideas into Hungarian economic thinking. He lived and worked in turbulent times, and historians have only been able to appreciate his activity in a limited manner. The work of this excellent economist has all but been forgotten, although he was of international stature. After a brief summary of Varga’s profile the study will demonstrate the lasting influence he has had in four areas — namely, business cycle research and national income estimations, the 1946 Hungarian stabilisation program, corporate profit, and consumption economics — and will go on to summarise his most important achievements.

Neurosurgical activity during COVID-19 pandemic: an experts' opinion from China, South Korea, Italy, the USA, Colombia, and the UK

2020 ◽  
Vol 64 (4) ◽  
Author(s):  
Marco M. Fontanella ◽  
Giorgio Saraceno ◽  
Ting Lei ◽  
Joshua B. Bederson ◽  
Namkyu You ◽  
...  
Keyword(s):  
South Korea ◽  
The Usa ◽  
The Uk

Spatial Hotspot Analysis of Bucharest’s Urban Heat Island (UHI) Using Modis Data

2018 ◽  
Vol 18 (1) ◽  
pp. 14-22 ◽  
Author(s):  
Georgiana Grigoraș ◽  
Bogdan Urițescu
Keyword(s):  
Surface Temperature ◽  
Air Temperature ◽  
Land Surface Temperature ◽  
Hot Spots ◽  
Land Surface ◽  
Hot Spot ◽  
Monitoring Network ◽  
Residential Areas ◽  
Hotspot Analysis ◽  
The City

Abstract The aim of the study is to find the relationship between the land surface temperature and air temperature and to determine the hot spots in the urban area of Bucharest, the capital of Romania. The analysis was based on images from both moderate-resolution imaging spectroradiometer (MODIS), located on both Terra and Aqua platforms, as well as on data recorded by the four automatic weather stations existing in the endowment of The National Air Quality Monitoring Network, from the summer of 2017. Correlation coefficients between land surface temperature and air temperature were higher at night (0.8-0.87) and slightly lower during the day (0.71-0.77). After the validation of satellite data with in-situ temperature measurements, the hot spots in the metropolitan area of Bucharest were identified using Getis-Ord spatial statistics analysis. It has been achieved that the “very hot” areas are grouped in the center of the city and along the main traffic streets and dense residential areas. During the day the "very hot spots” represent 33.2% of the city's surface, and during the night 31.6%. The area where the mentioned spots persist, falls into the "very hot spot" category both day and night, it represents 27.1% of the city’s surface and it is mainly represented by the city center.

Using augmented reality for navigation in space. Creating Visual Dictionaries

2020 ◽  
Author(s):  
Aida Khakimova ◽  
Oleg Zolotarev ◽  
Lyudmila Sharapova ◽  
Daler Mirzoev ◽  
Aleksanra Belaya ◽  
...  
Keyword(s):  
Augmented Reality ◽  
Visual Image ◽  
Cultural Codes ◽  
The Subject ◽  
Spatio Temporal ◽  
The City

The image of the city is a spatio-temporal continuum in which everything is interconnected, it exists as a single monolith expressing itself in the general atmosphere. The visual image of the city may contain two planes of meanings: culturally ratified and universally valid, expressed by cultural codes, and also significant only to those who are viewing the image. Therefore, the content of the visual image depends on who the subject of perception is, what he pays attention to and in what situation the process of perception of the image occurs.

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