The Diameter Factor of Aligned Electrospinning Membranes Facilitates Cutaneous Wound Healing by Promoting Epithelialization

AbstractThe re-epithelialization with appendages is a major challenge to skin injury. Given that topographical factors initially modulate cell activities, the design of biomaterials with the topography (such as pattern and diameter) offers a promising strategy for achieving optimal skin wound healing. Herein, we prepare an aligned fish collagen reinforced poly (lactide-co-glycolide) electrospinning membrane (FC/PLGA) with different fiber diameters including 319±100nm (A300), 687±138nm (A600) and 1048 ± 130nm (A1000) for promoting the re-epithelialization with appendages. The A300 significantly enhanced the mechanical strength and hydrophilicity, accelerated the degradation rate, and improved the cytocompatibility (proliferation and spreading) of the FC/PLGA membrane. In particular, the A300 facilitated skin wound healing (epithelization and vascularization) with the regeneration of hair follicles (HFs). The transcriptome revealed the underlying molecular mechanism that the A300 could promote macrophages transformation to reparative phenotypes (M2), subsequently exerting anti-inflammation and promoting epidermal cell migration via secreting IL10, TGFβ and MMP12. All the results indicate the A300 to be a potential candidate for guided tissue regeneration applications.Abstract Figure

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Sericin hydrogels promote skin wound healing with effective regeneration of hair follicles and sebaceous glands after complete loss of epidermis and dermis

Biomaterials Science ◽

10.1039/c8bm00934a ◽

2018 ◽

Vol 6 (11) ◽

pp. 2859-2870 ◽

Cited By ~ 24

Author(s):

Chao Qi ◽

Luming Xu ◽

Yan Deng ◽

Guobin Wang ◽

Zheng Wang ◽

...

Keyword(s):

Wound Healing ◽

Skin Wound ◽

Hair Follicles ◽

Complete Loss ◽

Full Thickness ◽

Sebaceous Glands ◽

Skin Injury ◽

Skin Wound Healing ◽

Full Thickness Skin ◽

Thickness Skin

Treating full-thickness skin injury with photo-crosslinkable sericin hydrogel for scarless regeneration with effective restoration of skin appendages.

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Ulmus Parvifolia Accelerate Skin Wound Healing by Regulating the Expression of MMPs and TGF-β

10.20944/preprints201911.0171.v1 ◽

2019 ◽

Author(s):

Min Cheol Kang ◽

Silvia Yumnam ◽

Woo Sung Park ◽

Hae Min So ◽

Ki Hyun Kim ◽

...

Keyword(s):

Wound Healing ◽

Growth Factor ◽

Transforming Growth Factor ◽

Skin Wound ◽

Root Bark ◽

Potential Candidate ◽

Skin Wound Healing ◽

Healing Effect ◽

Ulmus Parvifolia ◽

Wound Healing Effect

Ulmus species have been widely used in Korean folk medicine because of their anti-inflammatory and antimicrobial properties. We intended to investigate the wound healing effect of the powder of Ulmus parvifolia (UP) root bark in a mouse wound healing model. We also determined the mechanisms of effects of Ulmus parvifolia (UP) in skin and skin wound healing effect using keratinocyte model. in vivo experiments showed that the wound lesions in the mice decreased by U. parvifolia with 200 mesh size of root bark powder and significantly reduced by treatment with UP, compared with those treated with U. macrocarpa (UM). Results from in vitro experiments also revealed that UP extract promoted the migration of human skin keratinocytes. UP powder treatment upregulated the expression of the matrix metalloproteinase-2 and -9 protein and significantly increased transforming growth factor (TGF)-β levels. We confirmed that topical administration of the bark powder of exerted a significant effect on skin wound healing by upregulating the expression of MMP and transforming growth factor-β. TGF-β In, Our study suggests that U. parvifolia may be a potential candidate for skin wound healing including epidermal skin rejuvernation.

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Isoegomaketone from Perilla frutescens (L.) Britt Stimulates MAPK/ERK Pathway in Human Keratinocyte to Promote Skin Wound Healing

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/6642606 ◽

2021 ◽

Vol 2021 ◽

pp. 1-8

Author(s):

Ye-Ram Kim ◽

Bomi Nam ◽

Ah-Reum Han ◽

Jin-Baek Kim ◽

Chang Hyun Jin

Keyword(s):

Cell Proliferation ◽

Wound Healing ◽

Skin Wound ◽

Perilla Frutescens ◽

Potential Candidate ◽

Dependent Manner ◽

Erk Pathway ◽

Skin Wound Healing ◽

Therapeutic Benefits ◽

Human Keratinocyte

Skin wound healing is essential for recovery from injury, and delayed or impaired wound healing is a severe therapeutic challenge. Keratinocytes, a major component of the epidermis, play crucial roles in reepithelialization during wound healing including cell proliferation. Recent studies have shown that compounds from natural products have candidates for healing skin injury. Isoegomaketone (IK), isolated from leaves of Perilla frutescens var. crispa (Lamiaceae), has various bioactivities. However, the effect of IK on cutaneous wound healing processes has not been studied yet. In this study, we demonstrated that IK exhibits therapeutic wound healing effects using the human keratinocyte cell line HaCaT. Notably, IK promoted cell proliferation and migration in a dose-dependent manner in vitro, and treatment with 10 μM IK upregulated these processes by approximately 1.5-fold after 24 h compared with the control. IK induced the activation of the MAPK/ERK pathway and cell cycle progression to the S and G2/M phases. Thus, this study demonstrates IK as a potential candidate to upregulate wound healing that may provide therapeutic benefits to patients with delayed wound healing.

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Investigation of Healing Effects of Cinnamic Acid in a Full-Thickness Wound Model in Rabbit

Jundishapur Journal of Natural Pharmaceutical Products ◽

10.5812/jjnpp.97669 ◽

2021 ◽

Vol 16 (1) ◽

Author(s):

Mohammad Naghavi ◽

Pari Tamri ◽

Sara Soleimani Asl

Keyword(s):

Wound Healing ◽

Cinnamic Acid ◽

Wound Closure ◽

Antioxidant Properties ◽

Skin Wound ◽

Hair Follicles ◽

Vehicle Group ◽

Full Thickness ◽

Skin Wound Healing ◽

Tissue Samples

Background: Wounds represent a major global problem for health care systems, clinicians, patients, and their families. Cinnamic Acid (CA) is a naturally occurring phenolic compound that possesses anti-inflammatory, antimicrobial, and antioxidant properties. Objectives: This study aimed to investigate the effects of CA on skin wound-healing in the animal model. Methods: Full-thickness wounds were created on the back of white New Zealand rabbits of both sexes. Animals were divided into six groups (six animals and 12 wounds in each group). Negative control received no treatment, while positive control was treated with phenytoin cream, vehicle group with eucerin, and test groups with 0.1, 1, and 10% CA ointments. The healing activity of CA was evaluated by determining the wound closure rate and hydroxyproline content of wound tissue samples. In addition, the histopathological study of tissue samples of different groups was performed using hematoxylin and eosin staining. Results: The rate of wound closure and hydroxyproline levels of tissue samples in animals treated with CA 0.1% were significantly (P < 0.05) higher than those of no-treatment and vehicle-treated groups. Histological study revealed the increased number of fibroblasts and hair follicles, increased reepithelialization rate, and enhanced neovascularization in CA 0.1%-treated group when compared to no-treatment and vehicle groups. Conclusions: Cinnamic acid at low concentrations (< 1%) is potent for skin wound-healing and could be used as a safe and effective topical healing agent. Further studies are needed to confirm our findings.

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Photodynamic therapy accelerates skin wound healing through promoting re-epithelialization

Burns & Trauma ◽

10.1093/burnst/tkab008 ◽

2021 ◽

Vol 9 ◽

Author(s):

Zengjun Yang ◽

Xiaohong Hu ◽

Lina Zhou ◽

Yaxiong He ◽

Xiaorong Zhang ◽

...

Keyword(s):

Flow Cytometry ◽

Wound Healing ◽

Photodynamic Therapy ◽

Skin Wound ◽

Hair Follicles ◽

Skin Wound Healing ◽

And Migration ◽

Proliferation And Migration

Abstract Background Epidermal stem cells (EpSCs) that reside in cutaneous hair follicles and the basal layer of the epidermis are indispensable for wound healing and skin homeostasis. Little is known about the effects of photochemical activation on EpSC differentiation, proliferation and migration during wound healing. The present study aimed to determine the effects of photodynamic therapy (PDT) on wound healing in vivo and in vitro. Methods We created mouse full-thickness skin resection models and applied 5-aminolevulinic acid (ALA) for PDT to the wound beds. Wound healing was analysed by gross evaluation and haematoxylin–eosin staining in vivo. In cultured EpSCs, protein expression was measured using flow cytometry and immunohistochemistry. Cell migration was examined using a scratch model; apoptosis and differentiation were measured using flow cytometry. Results PDT accelerated wound closure by enhancing EpSC differentiation, proliferation and migration, thereby promoting re-epithelialization and angiogenesis. PDT inhibited inflammatory infiltration and expression of proinflammatory cytokines, whereas the secretion of growth factors was greater than in other groups. The proportion of transient amplifying cells was significantly greater in vivo and in vitro in the PDT groups. EpSC migration was markedly enhanced after ALA-induced PDT. Conclusions Topical ALA-induced PDT stimulates wound healing by enhancing re-epithelialization, promoting angiogenesis as well as modulating skin homeostasis. This work provides a preliminary theoretical foundation for the clinical administration of topical ALA-induced PDT in skin wound healing.

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Ulmus parvifolia Accelerates Skin Wound Healing by Regulating the Expression of MMPs and TGF-β

Journal of Clinical Medicine ◽

10.3390/jcm9010059 ◽

2019 ◽

Vol 9 (1) ◽

pp. 59

Author(s):

Min Cheol Kang ◽

Silvia Yumnam ◽

Woo Sung Park ◽

Hae Min So ◽

Ki Hyun Kim ◽

...

Keyword(s):

Wound Healing ◽

Growth Factor ◽

Transforming Growth Factor ◽

Skin Wound ◽

Matrix Metalloproteinase 2 ◽

Root Bark ◽

Potential Candidate ◽

Skin Wound Healing ◽

Ulmus Parvifolia ◽

Model Animal

Ulmus parvifolia is one of the medicinal plants used traditionally for treatment of wounds. We intended to investigate the wound healing effect of the powder of Ulmus parvifolia (UP) root bark in a mouse wound healing model. We also determined the mechanisms of effects of U. parvifolia in skin and skin wound healing effects using a keratinocyte model. Animal experiments showed that the wound lesions in the mice decreased with 200 mesh U. parvifolia root bark powder and were significantly reduced with treatment by UP, compared with those treated with Ulmus macrocarpa (UM). Results from in vitro experiments also revealed that UP extract promoted the migration of human skin keratinocytes. UP powder treatment upregulated the expression of the matrix metalloproteinase-2 and -9 protein and significantly increased transforming growth factor (TGF)-β levels. We confirmed that topical administration of the bark powder exerted a significant effect on skin wound healing by upregulating the expression of MMP and transforming growth factor-β. Our study suggests that U. parvifolia may be a potential candidate for skin wound healing including epidermal skin rejuvenation.

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Skin wound healing in the first generation (F1) offspring of Yorkshire and red Duroc pigs: Evidence for genetic inheritance of wound phenotype

Yearbook of Surgery ◽

10.1016/s0090-3671(08)70054-5 ◽

2007 ◽

Vol 2007 ◽

pp. 63-64

Author(s):

D.W. Mozingo

Keyword(s):

Wound Healing ◽

First Generation ◽

Skin Wound ◽

Genetic Inheritance ◽

Skin Wound Healing

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Overexpression of the Oral Mucosa-Specific microRNA-31 Promotes Skin Wound Closure

International Journal of Molecular Sciences ◽

10.3390/ijms20153679 ◽

2019 ◽

Vol 20 (15) ◽

pp. 3679 ◽

Cited By ~ 3

Author(s):

Lin Chen ◽

Alyne Simões ◽

Zujian Chen ◽

Yan Zhao ◽

Xinming Wu ◽

...

Keyword(s):

Wound Healing ◽

Oral Mucosa ◽

Wound Closure ◽

Expression Patterns ◽

Skin Wound ◽

Skin Wound Healing ◽

Specific Expression ◽

Keratinocyte Proliferation

Wounds within the oral mucosa are known to heal more rapidly than skin wounds. Recent studies suggest that differences in the microRNAome profiles may underlie the exceptional healing that occurs in oral mucosa. Here, we test whether skin wound-healing can be accelerating by increasing the levels of oral mucosa-specific microRNAs. A panel of 57 differentially expressed high expresser microRNAs were identified based on our previously published miR-seq dataset of paired skin and oral mucosal wound-healing [Sci. Rep. (2019) 9:7160]. These microRNAs were further grouped into 5 clusters based on their expression patterns, and their differential expression was confirmed by TaqMan-based quantification of LCM-captured epithelial cells from the wound edges. Of these 5 clusters, Cluster IV (consisting of 8 microRNAs, including miR-31) is most intriguing due to its tissue-specific expression pattern and temporal changes during wound-healing. The in vitro functional assays show that ectopic transfection of miR-31 consistently enhanced keratinocyte proliferation and migration. In vivo, miR-31 mimic treatment led to a statistically significant acceleration of wound closure. Our results demonstrate that wound-healing can be enhanced in skin through the overexpression of microRNAs that are highly expressed in the privileged healing response of the oral mucosa.

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The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System

Nanomaterials ◽

10.3390/nano11020457 ◽

2021 ◽

Vol 11 (2) ◽

pp. 457

Author(s):

Andreu Blanquer ◽

Jana Musilkova ◽

Elena Filova ◽

Johanka Taborska ◽

Eduard Brynda ◽

...

Keyword(s):

Wound Healing ◽

Endothelial Cells ◽

Chronic Wounds ◽

Human Keratinocytes ◽

Skin Wound ◽

Platelet Lysate ◽

Therapeutic Approaches ◽

Skin Wound Healing ◽

New Therapeutic Approaches ◽

Proliferation And Differentiation

Chronic wounds affect millions of patients worldwide, and it is estimated that this number will increase steadily in the future due to population ageing. The research of new therapeutic approaches to wound healing includes the development of nanofibrous meshes and the use of platelet lysate (PL) to stimulate skin regeneration. This study considers a combination of a degradable electrospun nanofibrous blend of poly(L-lactide-co-ε-caprolactone) and poly(ε-caprolactone) (PLCL/PCL) membranes (NF) and fibrin loaded with various concentrations of PL aimed at the development of bioactive skin wound healing dressings. The cytocompatibility of the NF membranes, as well as the effect of PL, was evaluated in both monocultures and co-cultures of human keratinocytes and human endothelial cells. We determined that the keratinocytes were able to adhere on all the membranes, and their increased proliferation and differentiation was observed on the membranes that contained fibrin with at least 50% of PL (Fbg + PL) after 14 days. With respect to the co-culture experiments, the membranes with fibrin with 20% of PL were observed to enhance the metabolic activity of endothelial cells and their migration, and the proliferation and differentiation of keratinocytes. The results suggest that the newly developed NF combined with fibrin and PL, described in the study, provides a promising dressing for chronic wound healing purposes.

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