scholarly journals Development of a Low-Cost System for Simultaneous Longitudinal Biological Imaging

2021 ◽  
Author(s):  
Victoria T Ly ◽  
Pierre V Baudin ◽  
Pattawong Pansodtee ◽  
Erik A Jung ◽  
Kateryna Voitiuk ◽  
...  
Keyword(s):  
Low Cost ◽  
Image Data ◽  
Biological Imaging ◽  
Cost System ◽  
Long Time ◽  
Culture Models ◽  
3D Printed ◽  
Longitudinal Imaging ◽  
Printed Materials ◽  
Multiple Conditions

Simultaneous longitudinal imaging across multiple conditions and replicates has been crucial for scientific studies aiming to understand biological processes and disease. Yet, imaging systems capable of accomplishing these tasks are economically unattainable for most academic and teaching laboratories around the world. Here we propose the Picroscope, which is the first low cost system for simultaneous longitudinal biological imaging made primarily using off-the-shelf and 3D-printed materials. The Picroscope is compatible with standard 24-well cell culture plates and captures 3D z-stack image data. The Picroscope can be controlled remotely, allowing for automatic imaging with minimal intervention from the investigator. Here we use this system in a range of applications. We gathered longitudinal whole organism image data for frogs, zebrafish and planaria worms.We also gathered image data inside an incubator to observe 2D monolayers and 3D mammalian tissue culture models. Using this tool, we can measure the behavior of entire organisms or individual cells over long time periods.

scholarly journals Picroscope: low-cost system for simultaneous longitudinal biological imaging

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Victoria T. Ly ◽  
Pierre V. Baudin ◽  
Pattawong Pansodtee ◽  
Erik A. Jung ◽  
Kateryna Voitiuk ◽  
...  
Keyword(s):  
Low Cost ◽  
Image Data ◽  
Biological Imaging ◽  
Cost System ◽  
Long Time ◽  
Culture Models ◽  
3D Printed ◽  
Longitudinal Imaging ◽  
Printed Materials ◽  
Multiple Conditions

AbstractSimultaneous longitudinal imaging across multiple conditions and replicates has been crucial for scientific studies aiming to understand biological processes and disease. Yet, imaging systems capable of accomplishing these tasks are economically unattainable for most academic and teaching laboratories around the world. Here, we propose the Picroscope, which is the first low-cost system for simultaneous longitudinal biological imaging made primarily using off-the-shelf and 3D-printed materials. The Picroscope is compatible with standard 24-well cell culture plates and captures 3D z-stack image data. The Picroscope can be controlled remotely, allowing for automatic imaging with minimal intervention from the investigator. Here, we use this system in a range of applications. We gathered longitudinal whole organism image data for frogs, zebrafish, and planaria worms. We also gathered image data inside an incubator to observe 2D monolayers and 3D mammalian tissue culture models. Using this tool, we can measure the behavior of entire organisms or individual cells over long-time periods.

FEASIBILITY ANALYSIS OF AN AUTOMATED DETECTION OF PHYSICAL DEFECT VIA COMPUTER VISION

2015 ◽  
Vol 76 (12) ◽  
Author(s):  
Por Jing Zhao ◽  
Shafriza Nisha Basah ◽  
Shazmin Aniza Abdul Shukor
Keyword(s):  
Computer Vision ◽  
Low Cost ◽  
Image Data ◽  
Automated Detection ◽  
Building Construction ◽  
High Demand ◽  
Major City ◽  
Cost System ◽  
Kinect Camera ◽  
Wall Condition

High demand of building construction has been taking places in the major city of Malaysia. However, despite this magnificent development, the lack of proper maintenance has caused a large portion of these properties deteriorated over time. The implementation of the project - Automated Detection of Physical Defect via Computer Vision - is a low cost system that helps to inspect the wall condition using Kinect camera. The system is able to classify the types of physical defects -crack and hole - and state its level of severity.The system uses artificial neural network as the image classifier due to its reliability and consistency. The validity of the system is shown using experiments on synthetic and real image data. This automated physical defect detection could detect building defect early, quickly, and easily, which results in cost saving and extending building life span. 

scholarly journals An Automated Behavioral Apparatus to Assess Distal Forelimb Function in Non-Human Primates

2018 ◽  
Author(s):  
Douglas Totten ◽  
Lisa Novik ◽  
Kari Christe ◽  
Marie-Josee Lemoy ◽  
Jeffrey Roberts ◽  
...  
Keyword(s):  
Low Cost ◽  
Reach To Grasp ◽  
Control Software ◽  
Temporal Precision ◽  
Cost System ◽  
Behavioral Cues ◽  
Temporal Cues ◽  
Electrophysiological Studies ◽  
3D Printed ◽  
Plastic Parts

ABSTRACTBackgroundPrimate distal forelimb behaviors are commonly assessed using reach-to-grasp tasks. While these tasks are widely recognized as sensitive assays for forelimb function, they often require experimenter input, lack precise temporal cues for physiological monitoring, and can be expensive.New MethodUsing components developed by open-source electronics platforms, we have designed and tested a low-cost system to measure distal forelimb function in non-human primates. Our system is inexpensive; it is made primarily of acrylic and 3D printed plastic parts. Our control software, developed in MATLAB, was also used to control two cameras in order to capture and process video during behavior. The system was equipped with sensors, motors and microcontrollers to control the timing of the task and facilitate synchronization between behavior and neurophysiology with high temporal precision.ResultsWe demonstrate that this system can be used to monitor motor recovery after stroke and investigate neurophysiological correlates of motor control.Comparison with Existing MethodsCompared to a previous version of this task, our setup reduces experimenter input while providing unbiased delivery of behavioral cues and behavioral measurements with the temporal precision necessary for electrophysiological studies.ConclusionsIn summary, our system will allow unbiased monitoring of forelimb function in both healthy and injured animals that is compatible with electrophysiological studies.

scholarly journals 3D Printing of Rapid, Low-Cost and Patient-Specific Models of Brain Vasculature for Use in Preoperative Planning in Clipping of Intracranial Aneurysms

2021 ◽  
Vol 10 (6) ◽  
pp. 1201
Author(s):  
Maciej Błaszczyk ◽  
Redwan Jabbar ◽  
Bartosz Szmyd ◽  
Maciej Radek
Keyword(s):  
3D Visualization ◽  
Low Cost ◽  
Cost Benefit ◽  
Image Data ◽  
Cost Effective ◽  
3D Models ◽  
Urgent Care ◽  
Patient Specific ◽  
Stl File ◽  
3D Printed

We developed a practical and cost-effective method of production of a 3D-printed model of the arterial Circle of Willis of patients treated because of an intracranial aneurysm. We present and explain the steps necessary to produce a 3D model from medical image data, and express the significant value such models have in patient-specific pre-operative planning as well as education. A Digital Imaging and Communications in Medicine (DICOM) viewer is used to create 3D visualization from a patient’s Computed Tomography Angiography (CTA) images. After generating the reconstruction, we manually remove the anatomical components that we wish to exclude from the print by utilizing tools provided with the imaging software. We then export this 3D reconstructions file into a Standard Triangulation Language (STL) file which is then run through a “Slicer” software to generate a G-code file for the printer. After the print is complete, the supports created during the printing process are removed manually. The 3D-printed models we created were of good accuracy and scale. The median production time used for the models described in this manuscript was 4.4 h (range: 3.9–4.5 h). Models were evaluated by neurosurgical teams at local hospital for quality and practicality for use in urgent and non-urgent care. We hope we have provided readers adequate insight into the equipment and software they would require to quickly produce their own accurate and cost-effective 3D models from CT angiography images. It has become quite clear to us that the cost-benefit ratio in the production of such a simplified model is worthwhile.

scholarly journals Assessing the Reusability of 3D-Printed Photopolymer Microfluidic Chips for Urine Processing

Micromachines ◽  
2018 ◽  
Vol 9 (10) ◽  
pp. 520 ◽  
Author(s):  
Eric Lepowsky ◽  
Reza Amin ◽  
Savas Tasoglu
Keyword(s):  
Protein Adsorption ◽  
Microfluidic Device ◽  
Low Cost ◽  
Three Dimensional ◽  
Device Fabrication ◽  
Microfluidic Chips ◽  
3D Printed ◽  
Printed Materials ◽  
Urine Processing ◽  
Nonspecific Protein Adsorption

Three-dimensional (3D) printing is emerging as a method for microfluidic device fabrication boasting facile and low-cost fabrication, as compared to conventional fabrication approaches, such as photolithography, for poly(dimethylsiloxane) (PDMS) counterparts. Additionally, there is an increasing trend in the development and implementation of miniaturized and automatized devices for health monitoring. While nonspecific protein adsorption by PDMS has been studied as a limitation for reusability, the protein adsorption characteristics of 3D-printed materials have not been well-studied or characterized. With these rationales in mind, we study the reusability of 3D-printed microfluidics chips. Herein, a 3D-printed cleaning chip, consisting of inlets for the sample, cleaning solution, and air, and a universal outlet, is presented to assess the reusability of a 3D-printed microfluidic device. Bovine serum albumin (BSA) was used a representative urinary protein and phosphate-buffered solution (PBS) was chosen as the cleaning agent. Using the 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde (CBQCA) fluorescence detection method, the protein cross-contamination between samples and the protein uptake of the cleaning chip were assessed, demonstrating a feasible 3D-printed chip design and cleaning procedure to enable reusable microfluidic devices. The performance of the 3D-printed cleaning chip for real urine sample handling was then validated using a commercial dipstick assay.

scholarly journals The Effect of 3D Printing Metal Materials on Osteoporosis Treatment

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Bing Wang ◽  
Chuwen Feng ◽  
Jianyu Pan ◽  
Shuoyan Zhou ◽  
Zhongren Sun ◽  
...  
Keyword(s):  
3D Printing ◽  
Relevant Literature ◽  
Osteoporosis Treatment ◽  
Rehabilitation Medicine ◽  
Physical And Rehabilitation Medicine ◽  
Long Time ◽  
3D Printed ◽  
Metal Materials ◽  
Printed Materials

3D printing has been in use for a long time and has continued to contribute to breakthroughs in the fields of clinical, physical, and rehabilitation medicine. In order to evaluate the role of 3D printing technology in treating spinal disorders, this paper presents a systematic review of the relevant literature. 3D printing is described in terms of its adjunctive function in various stages of spinal surgery and assistance in osteoporosis treatment. A review of metal 3D printed materials and applications of the technology is also provided.

Development of a Membraneless Organic Redox Flow Battery

2018 ◽  
Author(s):  
Korey Cook ◽  
Ethan Lau ◽  
Jordan Thayer ◽  
Shane Mann ◽  
Tom Guarr ◽  
...  
Keyword(s):  
Energy Storage ◽  
Low Cost ◽  
Storage System ◽  
Energy Storage System ◽  
Flow Cells ◽  
Target Cost ◽  
Selective Membrane ◽  
3D Printed ◽  
Printed Materials ◽  
Energy Grid

The development of a novel electrochemical energy storage system, specifically a redox flow batteries (RFB), is discussed in this work. It has the distinction of not requiring an ion-selective membrane due to novel chemical compounds. The techno-economic aspects of a low-cost 3D printed flow cell and system design tailored for a novel chemistry is discussed. The organic compounds employed are inexpensive, have a long lifespan, and as mentioned enable the system to be membraneless. All these substantially decrease the capital and maintenance costs. Suitable systems were developed and tested using chemically compatible 3D printed materials for the flow cells. The estimated cost per kWh is lower than the Department of Energy’s target cost of $150/kWh for grid storage capacity. A commercial scale system, rated for a 1 MW, 5-hour discharge time, has an estimated cost of $65/kWh. The proposed technology could revolutionize the energy storage industry and help with the construction of a more stable and efficient energy grid.

scholarly journals Design and Manufacturing of Si-Based Non-Oxide Cellular Ceramic Structures through Indirect 3D Printing

Materials ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 471
Author(s):  
Ghenwa El Chawich ◽  
Joelle El Hayek ◽  
Vincent Rouessac ◽  
Didier Cot ◽  
Bertrand Rebière ◽  
...  
Keyword(s):  
3D Printing ◽  
Fused Deposition Modeling ◽  
Surface Defects ◽  
Low Cost ◽  
Dip Coating ◽  
Surface Finishing ◽  
Oxide Ceramic ◽  
Fused Deposition ◽  
3D Printed ◽  
Printed Materials

Additive manufacturing of Polymer-Derived Ceramics (PDCs) is regarded as a disruptive fabrication process that includes several technologies such as light curing and ink writing. However, 3D printing based on material extrusion is still not fully explored. Here, an indirect 3D printing approach combining Fused Deposition Modeling (FDM) and replica process is demonstrated as a simple and low-cost approach to deliver complex near-net-shaped cellular Si-based non-oxide ceramic architectures while preserving the structure. 3D-Printed honeycomb polylactic acid (PLA) lattices were dip-coated with two preceramic polymers (polyvinylsilazane and allylhydridopolycarbosilane) and then converted by pyrolysis respectively into SiCN and SiC ceramics. All the steps of the process (printing resolution and surface finishing, cross-linking, dip-coating, drying and pyrolysis) were optimized and controlled. Despite some internal and surface defects observed by topography, 3D-printed materials exhibited a retention of the highly porous honeycomb shape after pyrolysis. Weight loss, volume shrinkage, roughness and microstructural evolution with high annealing temperatures are discussed. Our results show that the sacrificial mold-assisted 3D printing is a suitable rapid approach for producing customizable lightweight highly stable Si-based 3D non-oxide ceramics.

Influence of Printing Orientation on the Dynamic Characteristics and Vibration Behavior of 3D Printed Structures

2017 ◽  
Author(s):  
Kumar Vikram Singh ◽  
Fazeel Khan ◽  
Jacob Veta ◽  
Anil Kumar Singh
Keyword(s):  
3D Printing ◽  
Complex Modulus ◽  
Low Cost ◽  
Rapid Development ◽  
Vibration Behavior ◽  
Curve Fit ◽  
Development Cycle ◽  
3D Printed ◽  
Printed Materials ◽  
Printing Direction

Rapid development in the field of additive manufacturing, evidenced, in part, by the proliferation of low cost 3D printing, has accelerated the prototyping and design evaluation stages of the product development cycle. 3D printed structures have shown variations in their material properties as a function of the printing orientation. Moreover, thermoplastic materials which are often used as filament materials for 3D printing are known to have dependency on temperature, frequency and strain rate. Hence, the aim of this research is to estimate the variations in the complex modulus of the printed materials as a function of printing direction. This will allow an estimation of the variation in the vibration characteristics (natural frequencies, damping) of the printed structures as a function of printing direction. To this end, PLA beams were printed in four different orientations. A dynamic mechanical analyzer was used to measure mechanical properties of the printed beams. By using a curve fit method, the frequency and temperature dependent complex modulus is estimated. These complex moduli are used for estimating the eigenvalues of a non-dimensional beam. The observed variability in the vibration behavior as a function of the printing orientation is summarized here.

Low-Cost System Handles Patient Phone Calls

2007 ◽  
Vol 40 (11) ◽  
pp. 53
Author(s):  
BRUCE K. DIXON
Keyword(s):  
Low Cost ◽  
Cost System ◽  
Phone Calls
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