scholarly journals A Single Intranasal Dose of Bacterial Therapeutics to Calves Confers Longitudinal Modulation of the Nasopharyngeal Microbiota

2022 ◽  
Author(s):  
Samat Amat ◽  
Edouard Timsit ◽  
Matthew Workentine ◽  
Timothy Schwinghamer ◽  
Frank van der Meer ◽  
...  
Keyword(s):  
Structural Equation ◽  
Animal Health ◽  
Feedlot Cattle ◽  
Competitive Interactions ◽  
Equation Modeling ◽  
Antimicrobial Use ◽  
Bacterial Interactions ◽  
Bacterial Microbiota ◽  
Respiratory Microbiota ◽  
Intranasal Dose

To address the emergence of antimicrobial-resistant pathogens in livestock, microbiome-based strategies are increasingly being sought to reduce antimicrobial use. Here, we describe the intranasal application of bacterial therapeutics (BTs) for mitigating bovine respiratory disease (BRD) and used structural equation modeling to investigate the causal networks after BT application.  Beef cattle received i) an intranasal cocktail of previously characterized BT strains, ii) an injection of metaphylactic antibiotic tulathromycin or iii) intranasal saline. Despite being transient colonizers, inoculated BT strains induced longitudinal modulation of the nasopharyngeal bacterial microbiota while showing no adverse effect on animal health. The BT-mediated changes in bacteria included reduced diversity and richness and strengthened cooperative and competitive interactions. In contrast, tulathromycin increased bacterial diversity and antibiotic resistance, and disrupted bacterial interactions. Overall, a single intranasal dose of BTs can modulate the bovine respiratory microbiota, highlighting that microbiome-based strategies have the potential in being utilized to mitigate BRD in feedlot cattle.

Accounting for Data Architecture on Structural Equation Modeling of Feedlot Cattle Performance

2018 ◽  
Vol 23 (4) ◽  
pp. 529-549 ◽  
Author(s):  
Kessinee Chitakasempornkul ◽  
Michael W. Sanderson ◽  
Elva Cha ◽  
David G. Renter ◽  
Abigail Jager ◽  
...  
Keyword(s):  
Structural Equation Modeling ◽  
Structural Equation ◽  
Feedlot Cattle ◽  
Equation Modeling ◽  
Data Architecture ◽  
Cattle Performance

scholarly journals Bovine respiratory microbiota of feedlot cattle and its association with disease

2022 ◽  
Vol 53 (1) ◽  
Author(s):  
Jianmin Chai ◽  
Sarah F. Capik ◽  
Beth Kegley ◽  
John T. Richeson ◽  
Jeremy G. Powell ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Animal Health ◽  
Alternative Therapy ◽  
Clinical Signs ◽  
Feedlot Cattle ◽  
Global Food Security ◽  
Microbial Dysbiosis ◽  
Airway Microbiome ◽  
And Performance ◽  
Respiratory Microbiota

AbstractBovine respiratory disease (BRD), as one of the most common and costly diseases in the beef cattle industry, has significant adverse impacts on global food security and the economic stability of the industry. The bovine respiratory microbiome is strongly associated with health and disease and may provide insights for alternative therapy when treating BRD. The niche-specific microbiome communities that colonize the inter-surface of the upper and the lower respiratory tract consist of a dynamic and complex ecological system. The correlation between the disequilibrium in the respiratory ecosystem and BRD has become a hot research topic. Hence, we summarize the pathogenesis and clinical signs of BRD and the alteration of the respiratory microbiota. Current research techniques and the biogeography of the microbiome in the healthy respiratory tract are also reviewed. We discuss the process of resident microbiota and pathogen colonization as well as the host immune response. Although associations between the microbiota and BRD have been revealed to some extent, interpreting the development of BRD in relation to respiratory microbial dysbiosis will likely be the direction for upcoming studies, which will allow us to better understand the importance of the airway microbiome and its contributions to animal health and performance.

scholarly journals Manure Application Did Not Enrich Antibiotic Resistance Genes in Root Endophytic Bacterial Microbiota of Cherry Radish Plants

2019 ◽  
Vol 86 (2) ◽  
Author(s):  
Yu-Jing Zhang ◽  
Hang-Wei Hu ◽  
Qing-Lin Chen ◽  
Hui Yan ◽  
Jun-Tao Wang ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Structural Equation ◽  
Organic Fertilizer ◽  
Antibiotic Resistance Genes ◽  
Pot Experiment ◽  
Equation Modeling ◽  
Manure Application ◽  
Edible Part ◽  
Bacterial Microbiota

ABSTRACT Growing evidence suggests that livestock manure used as organic fertilizer in agriculture may lead to the potential propagation of antibiotic resistance genes (ARGs) from “farm to fork.” However, little is known about the impacts of manure fertilization on the incidence of ARGs in the plant-associated microbiomes (including rhizosphere, endosphere, and phyllosphere), which hampers our ability to assess the dissemination of antibiotic resistance in the soil-plant system. Here, we constructed a pot experiment to explore the effects of poultry and cattle manure applications on the shifts in the resistome in the plant microbiome of harvested cherry radish. A total of 144 ARGs conferring resistance to eight major classes of antibiotics were detected among all the samples. Rhizosphere and phyllosphere microbiomes harbored significantly higher diversity and abundance of ARGs than did root endophytic microbiomes of cherry radish. Manure application significantly increased the abundance of ARGs in the rhizosphere and phyllosphere but not in the endophytes of the root, which is the edible part of cherry radish. Soil and plant microbiomes changed dramatically after manure applications and clustered separately according to different sample types and treatments. Structural equation modeling revealed that bacterial abundance was the most important factor modulating the distribution patterns of soil and plant resistomes after accounting for multiple drivers. Taken together, we provide evidence that enrichment of the resistome in the rhizosphere and phyllosphere of cherry radish is more obvious than with the endosphere after manure application, suggesting that manure amendment might not enhance the dissemination of ARGs into the root of vegetables in the pot experiment. IMPORTANCE Our study provides important evidence that manure application increased the occurrence of ARGs in the rhizosphere and phyllosphere of cherry radish, compared with that in the endophytic bacterial microbiota of root, which is the edible part of cherry radish. Our findings suggest that although manure amendment is a significant route of ARGs entering agricultural soils, these manure-derived ARGs may be at low risk of migrating into the endophytes of root vegetables.

scholarly journals Modelling considerations in the analysis of associations between antimicrobial use and resistance in beef feedlot cattle

2015 ◽  
Vol 144 (6) ◽  
pp. 1313-1329 ◽  
Author(s):  
N. R. NOYES ◽  
K. M. BENEDICT ◽  
S. P. GOW ◽  
C. L. WALDNER ◽  
R. J. REID-SMITH ◽  
...  
Keyword(s):  
Goodness Of Fit ◽  
Research Question ◽  
Animal Health ◽  
Feedlot Cattle ◽  
Antimicrobial Use ◽  
Advantages And Disadvantages ◽  
Fit Statistics ◽  
Modelling Strategies ◽  
Modelling Methods ◽  
Modelling Approach

SUMMARYA number of sophisticated modelling approaches are available to investigate potential associations between antimicrobial use (AMU) and resistance (AMR) in animal health settings. All have their advantages and disadvantages, making it unclear as to which model is most appropriate. We used advanced regression modelling to investigate AMU-AMR associations in faecal non-type-specificEscherichia coli(NTSEC) isolates recovered from 275 pens of feedlot cattle. Ten modelling strategies were employed to investigate AMU associations with resistance to chloramphenicol, ampicillin, sulfisoxazole, tetracycline and streptomycin. Goodness-of-fit statistics did not show a consistent advantage for any one model type. Three AMU-AMR associations were significant in all models. Recent parenteral tetracycline use increased the odds of finding tetracycline-resistant NTSEC [odds ratios (OR) 1·1–3·2]; recent parenteral sulfonamide use increased the odds of finding sulfisoxazole-resistant NTSEC (OR 1·4–2·5); and recent parenteral macrolide use decreased the odds of recovering ampicillin-resistant NTSEC (OR 0·03–0·2). Other results varied markedly depending on the modelling approach, emphasizing the importance of exploring and reporting multiple modelling methods based on a balanced consideration of important factors such as study design, mathematical appropriateness, research question and target audience.

scholarly journals Feedlot Cattle Antimicrobial Use Surveillance Network: A Canadian Journey

2020 ◽  
Vol 7 ◽  
Author(s):  
Sherry J. Hannon ◽  
Stephanie A. Brault ◽  
Simon J. G. Otto ◽  
Paul S. Morley ◽  
Tim A. McAllister ◽  
...  
Keyword(s):  
Distribution System ◽  
Animal Health ◽  
Drug Distribution ◽  
Feedlot Cattle ◽  
Lessons Learned ◽  
Surveillance Program ◽  
Antimicrobial Use ◽  
Antimicrobial Drugs ◽  
Surveillance Network ◽  
Cattle Industry

Antimicrobial drugs are important tools for maintaining human and animal health. Globally, antimicrobial use (AMU) in food-producing animals is under increasing scrutiny due to its potential to promote antimicrobial resistance (AMR). Historically, comprehensive Canadian data related to the types of antimicrobial drugs used, extent of use, common indicators of use and the demographics of the cattle populations receiving antimicrobial drugs have been limited, in part due to segmentation in the cattle industry and fragmentation of the drug distribution system. Appropriate AMU estimates are required to understand AMU practices, to interpret AMR levels and patterns, to meaningfully assess associated public health risks, and to inform stewardship activities. The Canadian beef cattle industry has a long history of collaboration in AMU and AMR research. Prior research projects identified both opportunities and challenges in the collection of AMU data. Cornerstone projects provided insight into the complexity of collecting AMU data in Canada's feedlot sector. This paper will discuss how the lessons learned from past work have contributed to the formation of a Canadian fed-cattle antimicrobial surveillance program that was initiated in 2019. This important surveillance program will allow feedlot cattle AMU to improve management decisions and support AMU best practices in the evolving Canadian AMR landscape.

A Psychometric Study of the Quality Perception Questionnaire

2012 ◽  
Vol 71 (2) ◽  
pp. 101-106 ◽  
Author(s):  
Raffaele Cioffi† ◽  
Anna Coluccia ◽  
Fabio Ferretti ◽  
Francesca Lorini ◽  
Aristide Saggino ◽  
...  
Keyword(s):  
Structural Equation ◽  
Factor Model ◽  
Equation Modeling ◽  
Fit Indices ◽  
Quality Perception ◽  
Italian Survey ◽  
Confirmatory Factor ◽  
Instrument Measuring ◽  
Hospital Structures

The present paper reexamines the psychometric properties of the Quality Perception Questionnaire (QPQ), an Italian survey instrument measuring patients’ perceptions of the quality of a recent hospital admission experience, in a sample of 4400 patients (Mage = 56.42 years; SD = 19.71 years, 48.8% females). The 14-item survey measures four factors: satisfaction with medical doctors, nursing staff, auxiliary staff, and hospital structures. First, we tested two models using a confirmatory factor analysis (structural equation modeling): a four orthogonal factor and a four oblique factor model. The SEM fit indices and the χ² difference suggested the acceptance of the second model. We then did a simulation using a bootstrap with 1000 replications. Results confirmed the four oblique factor solution. Third, we tested whether there were significant differences with respect to age or sex. The multivariate general linear model showed no significant differences in the factors with respect to sex or age.

Suicide Risk Among College Student

Crisis ◽  
2015 ◽  
Vol 36 (6) ◽  
pp. 416-423 ◽  
Author(s):  
Richard Shadick ◽  
Faedra Backus Dagirmanjian ◽  
Baptiste Barbot
Keyword(s):  
Sexual Minority ◽  
Students Of Color ◽  
Structural Equation ◽  
Suicide Risk ◽  
Equation Modeling ◽  
First Year ◽  
Chi Square ◽  
First Year College ◽  
Marginalized Groups ◽  
First Year College Students

Abstract. Background: Research on young adults in the general population has identified a relationship between sexual minority identification and risk for suicide. Differential rates of suicidal ideation and attempts have also been found across racial and ethnic groups. Aims: This study examined risk for suicide among university students, based on membership in one or more marginalized groups (sexual minority and racial minority identification). Method: Data were collected from first-year college students (N = 4,345) at an urban university. Structural equation modeling was employed to model a suicidality construct, based on which a "risk for suicide" category system was derived. Chi-square and logistic regression analyses were then conducted to estimate the relationship between the background variables of interest and suicide risk. Results: Students who identified as lesbian, gay, or bisexual (LGB) were associated with higher suicide risk than their heterosexual peers. Students of color were slightly less at risk than their heterosexual peers. However, LGB students of color were associated with elevated suicide risk relative to heterosexual peers. Conclusion: Results indicate that belonging to multiple marginalized groups may increase one's risk for suicide, though these effects are not simply additive. Findings highlight the complexity of the intersection between marginalized identities and suicidality.

Low Self-Control of Emotions as an Antecedent of Self-Reported Physical Symptoms: A Longitudinal Perspective

2001 ◽  
Vol 6 (1) ◽  
pp. 26-35 ◽  
Author(s):  
Marja Kokkonen ◽  
Lea Pulkkinen ◽  
Taru Kinnunen
Keyword(s):  
Structural Equation ◽  
Negative Emotions ◽  
Physical Symptoms ◽  
Self Control ◽  
Personality Characteristics ◽  
Equation Modeling ◽  
Eysenck Personality Questionnaire ◽  
Positive Direction ◽  
Data Collections ◽  
Low Self Control

The study was part of the Jyväskylä Longitudinal Study of Personality and Social Development, underway since 1968, in which children's low self-control of emotions was studied using teacher ratings at age 8 in terms of inattentiveness, shifting moods, aggression, and anxiety. The study was based on data from 112 women and 112 men who participated in the previous data collections at ages 8, 27, and 36. At age 27, the participants had been assessed in Neuroticism (N) using the Eysenck Personality Questionnaire , and at age 36 they filled in several inventories measuring, among others, conscious and active attempts to repair negative emotions in a more positive direction as well as physical symptoms. The present study used structural equation modeling to test the hypothesis that personality characteristics indicating low self-control of emotions at ages 8 and 27 are antecedents of self-reported physical symptoms at age 36; and that this relationship is indirect, mediated by attempts to repair negative emotions in a more positive direction. The findings showed, albeit for men only, that inattentiveness at age 8 was positively related to self-reported physical symptoms at age 36 via high N at age 27 and low attempts to repair negative emotions at age 36. Additionally, N at age 27 was directly linked to self-reported physical symptoms at age 36. The mediation of an active attempt to repair negative emotions was not found for women. Correlations revealed, however, that shifting moods and aggression in girls were antecedents of self-reported physical symptoms in adulthood, particularly, pain and fatigue.

Validating the Factor Structure of the Stigma of Suicide Scale–Short Form Spanish Version Among Healthcare Students

Crisis ◽  
2020 ◽  
pp. 1-5
Author(s):  
Ruthmarie Hernández-Torres ◽  
Paola Carminelli-Corretjer ◽  
Nelmit Tollinchi-Natali ◽  
Ernesto Rosario-Hernández ◽  
Yovanska Duarté-Vélez ◽  
...  
Keyword(s):  
Factor Structure ◽  
Structural Equation ◽  
Short Form ◽  
Small Sample Size ◽  
Small Sample ◽  
Spanish Version ◽  
Equation Modeling ◽  
Cross Sectional ◽  
Healthcare Students ◽  
Spanish Speaking

Abstract. Background: Suicide is a leading cause of death among Spanish-speaking individuals. Suicide stigma can be a risk factor for suicide. A widely used measure is the Stigma of Suicide Scale-Short Form (SOSS-SF; Batterham, Calear, & Christensen, 2013 ). Although the SOSS-SF has established psychometric properties and factor structure in other languages and cultural contexts, no evidence is available from Spanish-speaking populations. Aim: This study aims to validate a Spanish translation of the SOSS-SF among a sample of Spanish-speaking healthcare students ( N = 277). Method: We implemented a cross-sectional design with quantitative techniques. Results: Following a structural equation modeling approach, a confirmatory factor analysis (CFA) supported the three-factor model proposed by Batterham and colleagues (2013) . Limitations: The study was limited by the small sample size and recruitment by availability. Conclusion: Findings suggest that the Spanish version of the SOSS-SF is a valid and reliable tool with which to examine suicide stigma among Spanish-speaking populations.

Risk Factors and Mediators of Suicidal Ideation Among Korean Adolescents

Crisis ◽  
2018 ◽  
Vol 39 (1) ◽  
pp. 4-12 ◽  
Author(s):  
Yi Jin Kim ◽  
Sung Seek Moon ◽  
Jang Hyun Lee ◽  
Joon Kyung Kim
Keyword(s):  
High School Students ◽  
Suicidal Ideation ◽  
Structural Equation ◽  
Equation Modeling ◽  
Outcome Variable ◽  
Suicidal Behaviors ◽  
Thwarted Belongingness ◽  
Perceived Burdensomeness ◽  
Korean Adolescents ◽  
Related Stress

Abstract. Background: A significant number of Korean adolescents have suicidal ideations and it is more prevalent among adolescents than any other age group in Korea. Aims: This study was conducted to attain a better understanding of the contributing factors to suicidal ideation among Korean adolescents. Method: We recruited 569 high school students in Grades 10 and 11 in Pyeongtaek, Korea. The Beck Scale for Suicidal Ideation was used to measure suicidal ideation as the outcome variable. The Interpersonal Needs Questionnaire, the Beck Hopelessness Scale, the School Related Stress Scale, the Olweus Bully/Victim Questionnaire, and the Youth Risk Behavior Surveillance questions were used to measure thwarted belongingness and perceived burdensomeness, hopelessness, school-related stress, bullying, and previous suicidal behaviors, respectively. Data analyses included descriptive statistics and structural equation modeling. Results: The findings suggest that perceived burdensomeness, hopelessness, school-related stress, and previous suicidal behaviors have significant direct effects on suicidal ideation. Hopelessness fully mediated the relation between thwarted belongingness and suicidal ideation, and partially mediated between perceived burdensomeness, school-related stress, and suicidal ideation. Conclusion: These findings provide more specific directions for a multidimensional suicide prevention program in order to be successful in reducing suicide rates among Korean adolescents.

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