scholarly journals Antibacterial effects of nanoimprinted moth-eye film in practical settings

2018 ◽  
Author(s):  
Miho Yamada ◽  
Kiyoshi Minoura ◽  
Takashi Mizoguchi ◽  
Kenichiro Nakamatsu ◽  
Tokio Taguchi ◽  
...  
Keyword(s):  
Antibacterial Effect ◽  
Culture Media ◽  
Bacterial Species ◽  
General Purpose ◽  
Antibacterial Effects ◽  
Hand Wash ◽  
Flat Film ◽  
Pet Film ◽  
Nanofabrication Technique

AbstractRecent studies report that surfaces displaying micrometer-or nanometer-sized undulating structures exhibit antibacterial effects. In previous work, we described the use of an advanced nanofabrication technique to generate an artificial biomimetic moth-eye film by coating a polyethylene terephthalate (PET) film with nanoscale moth-eye protrusions made from a hydrophilic resin. This moth-eye film exhibited enhanced antibacterial effects in in vitro experiments. The aim of the present study was to verify the antibacterial efficacy of the Moth-eye film in practical environments. Three types of films (Moth-eye film, Flat film, and PET film) were used to compare antibacterial effects. Sample films were pasted onto hand-wash sinks at the testing locations. After several hours of elapsed time, bacteria from the surface of sample films were collected using one of three kinds of culture media stampers (to permit identification of bacterial species). The stampers were incubated for 48 hours at 35 °C, and the numbers of colonies were counted.The number of common bacteria including E. coli and S. aureus from the Moth-eye film was significantly lower than that from the PET film (p<0.05) and that from the Flat film at 1 hour (p<0.05). This study found that the Moth-eye film had durability of antibacterial effect and the Moth-eye structure (PET coated with nanoscale cone-shaped pillars) had a physical antibacterial effect from the earlier time points. Therefore, the Moth-eye film might be useful for general-purpose applications in practical environments.

scholarly journals Synergistic antibacterial combination of Lavandula latifolia Medik. essential oil with camphor

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Nursenem Karaca ◽  
Görkem Şener ◽  
Betül Demirci ◽  
Fatih Demirci
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Antibacterial Effect ◽  
Synergistic Effects ◽  
Pharmaceutical Formulations ◽  
Human Pathogens ◽  
Broth Microdilution ◽  
Antibacterial Effects ◽  
Lavandula Latifolia

AbstractCombination of various compounds and essential oils for pharmaceutical formulations withdraw attention. In this present study, it was aimed to evaluate the in vitro potential synergistic antibacterial effect of Lavandula latifolia (spike lavender) essential oil with camphor by using the checkerboard method against the human pathogens; Staphylococcus aureus and Listeria monocytogenes. Pharmacopoeia quality L. latifolia essential oil and racemic camphor were analyzed and verified by GC-FID and GC/MS, simultaneously. In vitro antibacterial activity of essential oil and camphor (MIC range: 0.16–20 mg/mL) and standard antimicrobial clarithromycin (MIC range: 0.125–16 μg/mL) were carried out by broth microdilution against S. aureus and L. monocytogenes standard strains, respectively. Resulting antibacterial effects were evaluated for their fractional inhibitory concentrations (FICs) as antagonistic, additive and synergistic effects. The analytical results showed that the major component of essential oil was linalool (45.2%) and 1,8-cineole (25.6%). Antibacterial effects of essential oil were determined as MIC 1.25–5 mg/mL. As a result of the experiments, L. latifolia essential oil–camphor combinations were identified as “synergistic (FIC ≤ 0.5), and additive (0.5 < FIC ≤ 1)” in the respective combinations, suggesting further evaluation for formulations for potential antimicrobial applications in food and pharmaceuticals.

In vitro evaluation of the effect of nicotine, cotinine, and caffeine on oral microorganisms

2008 ◽  
Vol 54 (6) ◽  
pp. 501-508 ◽  
Author(s):  
Karina Cogo ◽  
Michelle Franz Montan ◽  
Cristiane de Cássia Bergamaschi ◽  
Eduardo D. Andrade ◽  
Pedro Luiz Rosalen ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Culture Media ◽  
Bacterial Species ◽  
In Vitro Study ◽  
Bacterial Strains ◽  
Planktonic Cells ◽  
Susceptibility Tests

The aim of this in vitro study was to evaluate the effects of nicotine, cotinine, and caffeine on the viability of some oral bacterial species. It also evaluated the ability of these bacteria to metabolize those substances. Single-species biofilms of Streptococcus gordonii , Porphyromonas gingivalis , or Fusobacterium nucleatum and dual-species biofilms of S. gordonii – F. nucleatum and F. nucleatum – P. gingivalis were grown on hydroxyapatite discs. Seven species were studied as planktonic cells, including Streptococcus oralis , Streptococcus mitis , Propionibacterium acnes , Actinomyces naeslundii , and the species mentioned above. The viability of planktonic cells and biofilms was analyzed by susceptibility tests and time-kill assays, respectively, against different concentrations of nicotine, cotinine, and caffeine. High-performance liquid chromatography was performed to quantify nicotine, cotinine, and caffeine concentrations in the culture media after the assays. Susceptibility tests and viability assays showed that nicotine, cotinine, and caffeine cannot reduce or stimulate bacterial growth. High-performance liquid chromatography results showed that nicotine, cotinine, and caffeine concentrations were not altered after bacteria exposure. These findings indicate that nicotine, cotinine, and caffeine, in the concentrations used, cannot affect significantly the growth of these oral bacterial strains. Moreover, these species do not seem to metabolize these substances.

scholarly journals Impact of Different Carbon Sources on the in vitro Growth and Viability of Escherichia coli (SUBE01) and Salmonella spp. (SUBS01) Cells

2016 ◽  
pp. 39-44
Author(s):  
Ifra Tun Nur ◽  
Jannatun Tahera ◽  
Md Sakil Munna ◽  
M Majibur Rahman ◽  
Rashed Noor
Keyword(s):  
Escherichia Coli ◽  
Bacterial Growth ◽  
Culture Media ◽  
Bacterial Species ◽  
Carbon Sources ◽  
Growth Dynamics ◽  
Luria Bertani ◽  
Tween 20 ◽  
Salmonella Spp

With a previous observation of Escherichia coli growth cessation along with temperature variation within three different bacteriological culture media (nutrient agar, Luria-Bertani agar and minimal agar), current investigation further depicted on the possible growth dynamics of Escherichia coli (SUBE01) and Salmonella (SUBS01) growth and viability upon supplementation of different carbon sources (dextrose, sucrose, lactose, glycerol and tween 20) at 37°C under the aeration of 100 rpm. Viability of the tested bacterial species was assessed through the enumeration of the colony forming unit (cfu) appeared upon prescribed incubation for 12-24 hours on different agar plates consisting of the above mentioned carbon sources. Besides, to inspect the cellular phenotypic changes, morphological observations were conducted under the light microscope. Variations in bacterial growth (either growth acceleration or cessation) were further noticed through the spot tests on the agar plates. Considerable shortfalls in the culturable cells of E. coli and Salmonella spp. were noted in the minimal media separately consisting of sucrose, lactose, glycerol or tween 20 while an opposite impact of accelerated growth was noticed in the media supplied with dextrose. The data revealed a hierarchy of consequence of carbon sources as nutrient generators whereby the favourable bacterial growth and survival order of the carbon sources was estimated as dextrose > glycerol > lactose > tween 20 > sucrose.Bangladesh J Microbiol, Volume 32, Number 1-2,June-Dec 2015, pp 39-44

scholarly journals Evaluation of the antimicrobial effects of atracurium, rocuronium and mivacurium. Antimicrobial effects of muscle relaxants

2010 ◽  
Vol 1 (1) ◽  
pp. 2 ◽  
Author(s):  
Volkan Hanci ◽  
Fusun Cömert ◽  
Hilal Ayoğlu ◽  
Canan Kulah ◽  
Serhan Yurtlu ◽  
...  
Keyword(s):  
Intensive Care ◽  
Antibacterial Effect ◽  
Neuromuscular Blocking ◽  
Antibacterial Effects ◽  
Minimum Concentration ◽  
Neuromuscular Blocking Drugs ◽  
Antimicrobial Effects ◽  
Anaesthetic Agents ◽  
Broth Microdilution Method

Some anaesthetic agents may be contaminated with microorganisms during the process of preparing an infusion. For this reason, it is important to understand the antimicrobial effects of various anaesthetic agents, which have been investigated to some degree in previous studies. However, studies specifically focusing on antibacterial effects of neuromuscular blocking drugs (anaesthetic agents) are very rare. Herein, we analysed the antimicrobial effects of atracurium, rocuronium, and mivacurium, on four different microorganisms. The in vitro antimicrobial activities of atracurium, rocuronium and mivacurium were investigated using the broth microdilution method. The pH of the test solutions was determined using a pH meter. The test microorganisms included Staphylococcus aureus ATCC 29213, Enterococcus fecalis ATCC 29212, Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853. The pH of the test solutions ranged between 7.20 and 7.32. The minimum inhibitory concentrations of atracurium, rocuronium and mivacurium for S. auereus, E. fecalis, E. coli and P. Aeruginosa were all found to be 512 µg/mL. Atracurium, rocuronium and mivacurium inhibit the growth of common intensive care unit pathogens at the same concentration (512 µg.mL–1). Thus, the neuromuscular blocking drugs, atracurium, rocuronium and mivacurium should be administered at a minimum concentration of 512 µg/mL in intensive care units to achieve this antibacterial effect. In our opinion, when used systemically, atracurium, rocuronium and mivacurium do not cause a systemic antibacterial effect. However, their antibacterial effects may be advantageous for inhibiting the spread of bacterial contamination during the preparation of the infusion solutions.

Combined Inhibitory Effect of Curcuma Xanthorrhiza Extract and Xylitol on Streptococcus Mutans and Actinomyces Viscosus

2007 ◽  
Vol 342-343 ◽  
pp. 861-864
Author(s):  
Hae Sun Kim ◽  
Choong Ho Choi ◽  
H.K. Kwon ◽  
B.I. Kim
Keyword(s):  
Antibacterial Effect ◽  
Bacterial Species ◽  
Inhibitory Effect ◽  
Distilled Water ◽  
Phenol Red ◽  
Ph Indicator ◽  
Bacterial Fermentation ◽  
Curcuma Xanthorrhiza ◽  
Growth Of Bacteria

This study evaluated the combined inhibitory effects of a Curcuma xanthorrhiza extract (CXE) and Xylitol on S. mutans and A. viscosus in vitro. Three series of experiments on S. mutans and A. viscosus were carried out. In the first series, the Minimum inhibitory concentrations (MICs) of CXE, Xylitol, and CXE mixed with Xylitol (CXE+Xylitol) against S. mutans and A. viscosus were determined. Second, the antibacterial effect and the rapid effectiveness of CXE, Xylitol, and CXE+Xylitol against those bacteria was evaluated as contacting for 1, 2, 5, and 10 minutes. Finally, The saccharolytic capability of S. mutans was examined using bovine teeth that had been pretreated with CXE (1%), Xylitol (1%), CXE+Xylitol (1%), chlorhexidine (1%) and distilled water, and rinsed with distilled water. The pretreated bovine teeth were layered with soft agar containing sucrose (5%), S. mutans and phenol red, as a pH indicator, and incubated. The MICs of CXE were 5 ppm on both bacterial species. Xylitol did not inhibit either species. The MICs of CXE+Xylitol were 10 and 5 ppm against S. mutans, A. viscosus, respectively. According to the rapid effectiveness, CXE completely inhibited the growth of bacteria but Xylitol did not. CXE+Xylitol could completely inhibit the growth of bacteria. An evaluation of the saccharolytic capability of S. mutans on bovine teeth revealed that distilled water and Xylitol could not inhibit bacterial fermentation. However, the bovine teeth containing CXE, CXE+Xylitol and chlorhexidine inhibited the fermentation of bacteria. These results show that CXE and CXE+Xylitol have a strong antibacterial effect on S. mutans and A. viscosus in vitro.

scholarly journals Microwave Mediated Synthesis of Novel 1,3,5-Triazine Derivative and their Biological Evaluation

2021 ◽  
Vol 3 (12) ◽  
pp. 181-185
Author(s):  
Krishn Kumar Barmase ◽  
Deepak Basedia ◽  
Balkrishna Dubey
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Effect ◽  
Bacterial Species ◽  
Biological Evaluation ◽  
Chloride Ions ◽  
Gram Negative ◽  
Triazine Derivative ◽  
Triazine Derivatives ◽  
In Vitro Antibacterial Activity

1,3,5-Triazine derivative are synthesized by replacement of chloride ions of Cyanuric chloride and 1,3,5-Triazine derivative are showing promising biologically activity such as Antibacterial, Antifungal, Antimalarial, Antivirus, Anticancer that’s why interest of researches on synthesis of 1,3,5-Triazine derivatives always in focused. The present study reported the synthesis of 2,4,6-Trisubstituted 1,3,5-Triazine derivatives by Microwave mediated Method which gave the desired result in less time with higher yield. The structure of 1,3,5-Triazine derivatives have been elucidated by Spectral of IR, NMR and MASS. The derivative of 1,3,5-Triazine are evaluated for their In vitro Antibacterial activity against Gram Positive and Gram Negative Bacterial species and shown good Antibacterial effect.

scholarly journals ANTIBACTERIAL ACTIVITY OF FRESH ONION AND GARLIC JUICES

2020 ◽  
Vol 20 (3) ◽  
pp. 705-712
Author(s):  
LUCIANA TEODORA ROTARU ◽  
MARIUS NOVAC ◽  
OANA NICOLAESCU
Keyword(s):  
Bacterial Resistance ◽  
Antibacterial Effect ◽  
Bacterial Species ◽  
Antimicrobial Properties ◽  
Chemotherapeutic Agents ◽  
Active Components ◽  
Docking Method ◽  
Therapeutic Properties ◽  
Species Being

Herbal extracts with antimicrobial potential represent an important research directive, in the current medical world, aiming to isolate active components, to develop new chemotherapeutic agents with applicability in the treatment or use as adjuvant therapy in infectious states. Antimicrobial properties of plants are conferred by their ability to synthesize certain secondary metabolites with relatively complex structures. The last century has been marked by sustained efforts to search for new natural compounds with antibacterial therapeutic properties, due to the gradual reduction in the number of effective allopathic antibiotics and the toxic effects of antibiotic residues. Numerous in vitro studies have shown that plants have antibacterial efficacy, discovering the importance of little-studied natural resources in this regard, as being effective in fighting against bacterial resistance and destroying bacterial agents. In this study, the antibacterial effect of the fresh onion and garlic juice was compared to the antibiotics of choice, using the diffusimetric agar method. Both plant products tested have antibacterial effect, the bacterial species being classified as sensitive to their action. The molecular docking method helps us to see the type of interaction between ligands and targets, allicin having no common binding site with antibiotics of choice.

scholarly journals Nanocluster Structure of Pistacia atlantica subsp. Kurdica Turpentine and Its Antibacterial Effects

2020 ◽  
Vol 18 ◽  
Author(s):  
Ramyar Rahimi Darehbagh ◽  
Rojin Ramezani ◽  
Afra Hosseinpanahi ◽  
Ahmad Fotoohi ◽  
Samaneh Rouhi
Keyword(s):  
Antibacterial Agents ◽  
Direct Relationship ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
In Vivo Studies ◽  
Antibacterial Effects ◽  
Pistacia Atlantica ◽  
Inhibition Zones

Background: Medicinal herbs such as Pistacia atlantica (P. atlantica) subsp. Kurdica have antimicrobial effects. The present study is aimed to investigate the nanocluster structure of P. atlantica subsp. Kurdica turpentine and its composing elements and antibacterial effect. Methods: 100 μl ethanol was used to dissolve oily turpentine. 2, 2.2, 2.4, and 2.6 µg/μl of turpentine were used for investigating the antibacterial effects using disk and well diffusion methods. Elemental and nanocluster structure analyses were performed by Energy-Dispersive XRay Microanalysis (EDXMA) and Field Emission (FE)-scanning electron. Two-way analysis of variance (ANOVA) and Bonferoni test were used for data analysis (p ≤ 0.001). Results and Discussion: EDXMA elemental analysis of turpentine included: zinc (Zn), magnesium (Mg), fluorine (F), oxygen (O), silicium (Si), carbon (C), and argentum (Ag). A topography image of the turpentine showed a nanocluster surface with bright clusters in the background. The largest diameters of the growth inhibition zones (24.67 ± 0.58 mm in the disk diffusion and 23.67 ± 1.53 mm in the well diffusion) that were created by turpentine were observed against S. aureus ATCC 25923 at the concentration of 2.6 µg/μl. Diameter of the inhibition zone around bacterial growth had a direct relationship with turpentine concentration (p ≤ 0.001). Conclusion: The nanocluster structure of turpentine and its composed elements were detected in this research. Moreover, antibacterial effects of turpentine were proved. Herbal substances are widely used in medical applications. Different elements of P. atlantica subsp. Kurdica turpentine can be used as antibacterial agents, but more in-vitro and in-vivo studies should be performed in this field.

Activité antimicrobienne d'antioxydants phénoliques

1978 ◽  
Vol 24 (11) ◽  
pp. 1306-1320 ◽  
Author(s):  
Pierre Turcotte ◽  
Samir A. Saheb
Keyword(s):  
Culture Media ◽  
Osmotic Shock ◽  
Bacterial Species ◽  
Butylated Hydroxytoluene ◽  
In Vitro Assays ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
The Family ◽  
Increased Sensitivity

The antimicrobial activity of three antioxydants, butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA) and ethoxyquin (ETO) was studied. In vitro assays showed that when these antioxydants are added to the culture media at concentrations lower or equal to that used in nutrition, they inhibit or decrease the growth of certain microorganisms. BHT showed the most marked effect, affecting Gram-positive bacteria at a higher degree than the Gram-negative bacteria belonging to the family Enterobacteriaceae. Inactivation study of different bacterial species by BHT revealed differences in sensitivity among a single genus and between strains of the same species. The association of ETO with BHT results in an increase of the inhibitory activity. The increased sensitivity to BHT resulting from the osmotic shock of Escherichia coli cells suggests that the resistance to BHT of the Gram-negative bacteria belonging to the family Enterobacteriaceae might be due in part to the structure of their cell wall.

scholarly journals Systematic Culturomics Shows that Half of Chicken Caecal Microbiota Members can be Grown in Vitro Except for Two Lineages of Clostridiales and a Single Lineage of Bacteroidetes

2019 ◽  
Vol 7 (11) ◽  
pp. 496 ◽  
Author(s):  
Magdalena Crhanova ◽  
Daniela Karasova ◽  
Helena Juricova ◽  
Jitka Matiasovicova ◽  
Eva Jahodarova ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Culture Media ◽  
Bacterial Species ◽  
Disease Status ◽  
Epidemiological Data ◽  
Culture Conditions ◽  
Simple Task ◽  
Selective Enrichment ◽  
Caecal Microbiota

Epidemiological data show that the composition of gut microbiota influences host health, disease status, and even behaviour. However, to confirm these epidemiological observations in controlled experiments, pure cultures of gut anaerobes must be obtained. Since the culture of gut anaerobes is not a simple task due to the large number of bacterial species colonising the intestinal tract, in this study we inoculated 174 different culture media with caecal content from adult hens, and compared the microbiota composition in the original caecal samples and in bacterial masses growing in vitro by 16S rRNA sequencing. In total, 42% of gut microbiota members could be grown in vitro and since there were some species which were not cultured but for which the culture conditions are known, it is likely that more than half of chicken gut microbiota can be grown in vitro. However, there were two lineages of Clostridiales and a single lineage of Bacteroidetes which were common in chicken caecal microbiota but resistant to culture. Of the most selective culture conditions, nutrient broths supplemented with mono- or di-saccharides, including those present in fruits, positively selected for Lactobacillaceae. The addition of bile salts selected for Veillonellaceae and YCFA (yeast casitone fatty acid agar) enriched for Desulfovibrionaceae. In addition, Erysipelotrichaceae were positively selected by colistin, trimethoprim, streptomycin and nalidixic acid. Culture conditions tested in this study can be used for the selective enrichment of desired bacterial species but also point towards the specific functions of individual gut microbiota members.

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