scholarly journals Microclimate predicts frost-hardiness of alpine Arabidopsis thaliana populations better than altitude because the microclimate effect increases with altitude

2019 ◽  
Author(s):  
Christian Lampei ◽  
Jörg Wunder ◽  
Thomas Wilhalm ◽  
Karl J. Schmid
Keyword(s):  
Arabidopsis Thaliana ◽  
Large Scale ◽  
Frost Damage ◽  
Rate Of Change ◽  
Frost Hardiness ◽  
List Type ◽  
Field Station ◽  
Log Linear ◽  
Alpine Environments ◽  
Collection Sites

AbstractIn mountain regions average temperatures decrease at higher altitudes. In addition, microenvironmental conditions can strongly affect microclimate and may counteract average effects of altitude.We investigated winter frost hardiness of Arabidopsis thaliana accessions originating from 13 sites along altitudinal gradients in the Southern Alps during three winters on an experimental field station on the Swabian Jura and compared levels of frost damage with the observed number of frost days (<1°C) in eight collection sites.We found that frost-hardiness increased with altitude in a log-linear fashion. This is consistent with adaptation to higher frequency of frost conditions, but also indicates a decreasing rate of change in frost hardiness with increasing altitude. Moreover, the number of frost days measured with temperature loggers at the original collection sites correlated much better with frost-hardiness than the altitude of collection sites, suggesting that populations were adapted to their local microclimate. Notably, the variance in frost days across sites increased exponentially with altitude.Together, our results suggest that strong microclimate heterogeneity of high alpine environments may preserve functional genetic diversity in small populations. This challenges the suitability of habitat predictions based on large scale climatic variables (or proxies, such as altitude) for topographically complex areas.

scholarly journals Full species-wide leaf and seed ionomic diversity of Arabidopsis thaliana

2020 ◽  
Author(s):  
Ana Carolina A. L. Campos ◽  
William F.A. van Dijk ◽  
Priya Ramakrishna ◽  
Tom Giles ◽  
Pamela Korte ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
High Throughput ◽  
Large Scale ◽  
Natural Variation ◽  
Essential Elements ◽  
Soil Conditions ◽  
List Type ◽  
Digital Tool ◽  
Entire Collection ◽  
Nutritional Needs

SummarySoil is a heterogenous reservoir of essential elements needed for plant growth and development. Plants have evolved mechanisms to balance their nutritional needs based on availability of nutrients. This has led to genetically-based variation in the elemental composition ‘ionome’, of plants, both within and between species.We explore this natural variation using a panel of wild-collected, geographically widespread Arabidopsis thaliana accessions from the 1001 Genomes Project including over 1,135 accessions, and the 19 parental accessions of the Multi-parent Advanced Generation Inter-Cross (MAGIC) panel, all with full-genome sequences available.We present an experimental design pipeline for high-throughput ionomic screenings and analyses with improved normalisation procedures to account for errors and variability in conditions often encountered in large-scale, high-throughput data collection. We report quantification of the complete leaf and seed ionome of the entire collection using this pipeline and a digital tool-IonExplorer to interact with the dataset.We describe the pattern of natural ionomic variation across the A. thaliana species and identify several accessions with extreme ionomic profiles. It forms a valuable resource for exploratory QTL, GWA studies to identify genes underlying natural variation in leaf and seed ionome and genetic adaptation of plants to soil conditions.

Near-wake behaviour of a utility-scale wind turbine

2018 ◽  
Vol 859 ◽  
pp. 204-246 ◽  
Author(s):  
Teja Dasari ◽  
Yue Wu ◽  
Yun Liu ◽  
Jiarong Hong
Keyword(s):  
Wind Turbine ◽  
Large Scale ◽  
Velocity Ratio ◽  
Rate Of Change ◽  
Tip Vortex ◽  
Time Duration ◽  
Near Wake ◽  
Field Station ◽  
Operation Conditions ◽  
Utility Scale

Super-large-scale particle image velocimetry (SLPIV) and the associated flow visualization technique using natural snowfall have been shown to be effective tools to probe the turbulent velocity field and coherent structures around utility-scale wind turbines (Hong et al.Nat. Commun., vol. 5, 2014, article 4216). Here, we present a follow-up study using the data collected during multiple deployments from 2014 to 2016 around the 2.5 MW turbine at the EOLOS field station. These data include SLPIV measurements in the near wake of the turbine in a field of view of 115 m (vertical) $\times$ 66 m (streamwise), and the visualization of tip vortex behaviour near the elevation corresponding to the bottom blade tip over a broad range of turbine operational conditions. The SLPIV measurements provide velocity deficit and turbulent kinetic energy assessments over the entire rotor span. The instantaneous velocity fields from SLPIV indicate the presence of intermittent wake contraction states which are in clear contrast with the expansion states typically associated with wind turbine wakes. These contraction states feature a pronounced upsurge of velocity in the central portion of the wake. The wake velocity ratio $R_{w}$, defined as the ratio of the spatially averaged velocity of the inner wake to that of the outer wake, is introduced to categorize the instantaneous near wake into expansion ($R_{w}<1$) and contraction states ($R_{w}>1$). Based on the $R_{w}$ criterion, the wake contraction occurs 25 % of the time during a 30 min time duration of SLPIV measurements. The contraction states are found to be correlated with the rate of change of blade pitch by examining the distribution and samples of time sequences of wake states with different turbine operation parameters. Moreover, blade pitch change is shown to be strongly correlated to the tower and blade strains measured on the turbine, and the result suggests that the flexing of the turbine tower and the blades could indeed lead to the interaction of the rotor with the turbine wake, causing wake contraction. The visualization of tip vortex behaviour demonstrates the presence of a state of consistent vortex formation as well as various types of disturbed vortex states. The histograms corresponding to the consistent and disturbed states are examined over a number of turbine operation/response parameters, including turbine power and tower strain as well as the fluctuation of these quantities, with different conditional sampling restrictions. This analysis establishes a clear statistical correspondence between these turbine parameters and tip vortex behaviours under different turbine operation conditions, which is further substantiated by examining samples of time series of these turbine parameters and tip vortex patterns. This study not only offers benchmark datasets for comparison with the-state-of-the-art numerical simulation, laboratory and field measurements, but also sheds light on understanding wake characteristics and the downstream development of the wake, turbine performance and regulation, as well as developing novel turbine or wind farm control strategies.

New Harvesting and Curing Techniques in the Production of Breeder's Seed Peanuts1

1979 ◽  
Vol 6 (2) ◽  
pp. 70-72
Author(s):  
T. A. Coffelt ◽  
F. S. Wright ◽  
J. L. Steele
Keyword(s):  
Large Scale ◽  
Combine Method ◽  
Frost Damage ◽  
New Method ◽  
Experimental Equipment ◽  
Harvesting Method ◽  
Curing Methods ◽  
Labor Requirements ◽  
High Level ◽  
Varietal Purity

Abstract A new method of harvesting and curing breeder's seed peanuts in Virginia was initiated that would 1) reduce the labor requirements, 2) maintain a high level of germination, 3) maintain varietal purity at 100%, and 4) reduce the risk of frost damage. Three possible harvesting and curing methods were studied. The traditional stack-pole method satisfied the latter 3 objectives, but not the first. The windrow-combine method satisfied the first 2 objectives, but not the last 2. The direct harvesting method satisfied all four objectives. The experimental equipment and curing procedures for direct harvesting had been developed but not tested on a large scale for seed harvesting. This method has been used in Virginia to produce breeder's seed of 3 peanut varieties (Florigiant, VA 72R and VA 61R) during five years. Compared to the stackpole method, labor requirements have been reduced, satisfactory levels of germination and varietal purity have been obtained, and the risk of frost damage has been minimized.

scholarly journals Effects of Climatic Drivers and Teleconnections on Late 20th Century Trends in Spring Freshet of Four Major Arctic-Draining Rivers

Water ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 179
Author(s):  
Roxanne Ahmed ◽  
Terry Prowse ◽  
Yonas Dibike ◽  
Barrie Bonsal
Keyword(s):  
Arctic Ocean ◽  
Large Scale ◽  
Southern Oscillation ◽  
Rate Of Change ◽  
The Arctic ◽  
Late 20Th Century ◽  
Basin Scale ◽  
The Arctic Ocean ◽  
Spring Freshet ◽  
Climatic Drivers

Spring freshet is the dominant annual discharge event in all major Arctic draining rivers with large contributions to freshwater inflow to the Arctic Ocean. Research has shown that the total freshwater influx to the Arctic Ocean has been increasing, while at the same time, the rate of change in the Arctic climate is significantly higher than in other parts of the globe. This study assesses the large-scale atmospheric and surface climatic conditions affecting the magnitude, timing and regional variability of the spring freshets by analyzing historic daily discharges from sub-basins within the four largest Arctic-draining watersheds (Mackenzie, Ob, Lena and Yenisei). Results reveal that climatic variations closely match the observed regional trends of increasing cold-season flows and earlier freshets. Flow regulation appears to suppress the effects of climatic drivers on freshet volume but does not have a significant impact on peak freshet magnitude or timing measures. Spring freshet characteristics are also influenced by El Niño-Southern Oscillation, the Pacific Decadal Oscillation, the Arctic Oscillation and the North Atlantic Oscillation, particularly in their positive phases. The majority of significant relationships are found in unregulated stations. This study provides a key insight into the climatic drivers of observed trends in freshet characteristics, whilst clarifying the effects of regulation versus climate at the sub-basin scale.

Comparative physical mapping reveals features of microsynteny between Glycine max, Medicago truncatula, and Arabidopsis thaliana

Genome ◽  
2004 ◽  
Vol 47 (1) ◽  
pp. 141-155 ◽  
Author(s):  
H H Yan ◽  
J Mudge ◽  
D-J Kim ◽  
R C Shoemaker ◽  
D R Cook ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Glycine Max ◽  
Medicago Truncatula ◽  
Physical Mapping ◽  
Large Scale ◽  
Sequence Similarity ◽  
Artificial Chromosome ◽  
Bac Contig ◽  
Cross Hybridization ◽  
Bac Contigs

To gain insight into genomic relationships between soybean (Glycine max) and Medicago truncatula, eight groups of bacterial artificial chromosome (BAC) contigs, together spanning 2.60 million base pairs (Mb) in G. max and 1.56 Mb in M. truncatula, were compared through high-resolution physical mapping combined with sequence and hybridization analysis of low-copy BAC ends. Cross-hybridization among G. max and M. truncatula contigs uncovered microsynteny in six of the contig groups and extensive microsynteny in three. Between G. max homoeologous (within genome duplicate) contigs, 85% of coding and 75% of noncoding sequences were conserved at the level of cross-hybridization. By contrast, only 29% of sequences were conserved between G. max and M. truncatula, and some kilobase-scale rearrangements were also observed. Detailed restriction maps were constructed for 11 contigs from the three highly microsyntenic groups, and these maps suggested that sequence order was highly conserved between G. max duplicates and generally conserved between G. max and M. truncatula. One instance of homoeologous BAC contigs in M. truncatula was also observed and examined in detail. A sequence similarity search against the Arabidopsis thaliana genome sequence identified up to three microsyntenic regions in A. thaliana for each of two of the legume BAC contig groups. Together, these results confirm previous predictions of one recent genome-wide duplication in G. max and suggest that M. truncatula also experienced ancient large-scale genome duplications.Key words: Glycine max, Medicago truncatula, Arabidopsis thaliana, conserved microsynteny, genome duplication.

scholarly journals Equatorial ionospheric disturbance observed through a transequatorial HF propagation experiment

2006 ◽  
Vol 24 (5) ◽  
pp. 1401-1409 ◽  
Author(s):  
T. Maruyama ◽  
M. Kawamura
Keyword(s):  
Large Scale ◽  
Ionospheric Disturbance ◽  
Great Circle ◽  
Rate Of Change ◽  
Spatial Distance ◽  
Propagation Path ◽  
Multiple Signal Classification ◽  
Radio Broadcasting ◽  
Equatorial Plasma Bubbles ◽  
Propagation Experiment

Abstract. A transequatorial radio-wave propagation experiment at shortwave frequencies (HF-TEP) was done between Shepparton, Australia, and Oarai, Japan, using the radio broadcasting signals of Radio Australia. The receiving facility at Oarai was capable of direction finding based on the MUSIC (Multiple Signal Classification) algorithm. The results were plotted in azimuth-time diagrams (AT plots). During the daytime, the propagation path was close to the great circle connecting Shepparton and Oarai, thus forming a single line in the AT plots. After sunset, off-great-circle paths, or satellite traces in the AT plot, often appeared abruptly to the west and gradually returned to the great circle direction. However, there were very few signals across the great circle to the east. The off-great-circle propagation was very similar to that previously reported and was attributed to reflection by an ionospheric structure near the equator. From the rate of change in the direction, we estimated the drift velocity of the structure to range mostly from 100 to 300 m/s eastward. Multiple instances of off-great-circle propagation with a quasi-periodicity were often observed and their spatial distance in the east-west direction was within the range of large-scale traveling ionospheric disturbances (LS-TIDs). Off-great-circle propagation events were frequently observed in the equinox seasons. Because there were many morphological similarities, the events were attributed to the onset of equatorial plasma bubbles.

Evidence for a large-scale population structure among accessions of Arabidopsis thaliana: possible causes and consequences for the distribution of linkage disequilibrium

2006 ◽  
Vol 15 (6) ◽  
pp. 1507-1517 ◽  
Author(s):  
MARIE-FRANCE OSTROWSKI ◽  
JACQUES DAVID ◽  
SYLVAIN SANTONI ◽  
HEATHER MCKHANN ◽  
XAVIER REBOUD ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Population Structure ◽  
Linkage Disequilibrium ◽  
Large Scale ◽  
Scale Population

scholarly journals Large-scale docking predicts that sORF-encoded peptides may function through protein-peptide interactions in Arabidopsis thaliana

PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0205179 ◽  
Author(s):  
Rashmi R. Hazarika ◽  
Nikolina Sostaric ◽  
Yifeng Sun ◽  
Vera van Noort
Keyword(s):  
Arabidopsis Thaliana ◽  
Large Scale ◽  
Peptide Interactions

scholarly journals In Vivo CRISPR Screens Identify E3 Ligase Cop1 as a Modulator of Macrophage Infiltration and Cancer Immunotherapy Target

2020 ◽  
Author(s):  
Xiaoqing Wang ◽  
Collin Tokheim ◽  
Binbin Wang ◽  
Shengqing Stan Gu ◽  
Qin Tang ◽  
...  
Keyword(s):  
Tumor Microenvironment ◽  
Cancer Cells ◽  
Cancer Immunotherapy ◽  
Tumor Immunity ◽  
Large Scale ◽  
Integrated Approach ◽  
Immune Checkpoint Blockade ◽  
Macrophage Infiltration ◽  
List Type

SUMMARYDespite remarkable clinical efficacies of immune checkpoint blockade (ICB) in cancer treatment, ICB benefits in triple-negative breast cancer (TNBC) remain limited. Through pooled in vivo CRISPR knockout (KO) screens in syngeneic TNBC mouse models, we found that inhibition of the E3 ubiquitin ligase Cop1 in cancer cells decreases the secretion of macrophage-associated chemokines, reduces tumor macrophage infiltration, and shows synergy in anti-tumor immunity with ICB. Transcriptomics, epigenomics, and proteomics analyses revealed Cop1 functions through proteasomal degradation of the C/ebpδ protein. Cop1 substrate Trib2 functions as a scaffold linking Cop1 and C/ebpδ, which leads to polyubiquitination of C/ebpδ. Cop1 inhibition stabilizes C/ebpδ to suppress the expression of macrophage chemoattractant genes. Our integrated approach implicates Cop1 as a target for improving cancer immunotherapy efficacy by regulating chemokine secretion and macrophage levels in the TNBC tumor microenvironment.HighlightsLarge-scale in vivo CRISPR screens identify new immune targets regulating the tumor microenvironmentCop1 knockout in cancer cells enhances anti-tumor immunityCop1 modulates chemokine secretion and macrophage infiltration into tumorsCop1 targets C/ebpδ degradation via Trib2 and influences ICB response

scholarly journals Screening and potent applicability analysis of commonly used pesticides against desert locust: an integrative entomo-informatics approach

2020 ◽  
Author(s):  
Anik Banik ◽  
Md. Fuad Mondal ◽  
Md. Mostafigur Rahman Khan ◽  
Sheikh Rashel Ahmed ◽  
Md. Mehedi Hasan
Keyword(s):  
Binding Affinity ◽  
Large Scale ◽  
Schistocerca Gregaria ◽  
Indian Subcontinent ◽  
Locusta Migratoria ◽  
Field Trials ◽  
The Other ◽  
List Type ◽  
Agricultural Pesticides ◽  
Global Threat

AbstractThe locust problem is a global threat for food security. Locusts can fly and migrate overseas within a zip and creating a large-scale devastation to the diversified agro-ecosystem. GIS based analysis showed the recent movement of locusts, among them Schistocerca gregaria and Locusta migratoria are predominant in Indian subcontinent and are found more notorious and devastating one. This devastation needs to be stopped to save human race from food deprivation. In our study, we screened some commonly used agricultural pesticides and strongly recommended three of them viz. biphenthrin, diafenthiuron and silafluofen which might be potential to control the desert locusts based on their binding affinity towards the locust’s survival proteins. Our phylogenetic analysis reveals that these three recommended pesticides might also show potency to the other locust species as well as they are also way safer than the other commercially available pesticides. These proposed pesticide’s bioactive analogs from fungus and bacteria may also show efficacy as next generation controlling measures of locust as well as different kind of pests. These recommended pesticides are expected to be highly effective against locusts and needs to bring forward by the entomologists’ by performing experimental field trials.HighlightsGIS map unmasked the 2020 migratory pattern of locusts which now predominant towards Indian subcontinent.Biphenthrin, diafenthiuron and silafluofen showed maximum binding affinity.Biphenthrin and diafenthiuron were relatively safer than silafluofen.Bioactive analogs from fungus and bacteria could be an alternative to control locusts.Pesticides inhibition hotspots for desert locusts were unrevealed.

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