Purification, crystallization and room-temperature X-ray diffraction of inositol dehydrogenase LcIDH2 fromLactobacillus caseiBL23
2014 ◽
Vol 70
(7)
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pp. 979-983
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Lactobacillus caseiBL23 contains two genes,iolG1andiolG2, homologous with inositol dehydrogenase encoding genes from many bacteria. Inositol dehydrogenase catalyzes the oxidation of inositol with concomitant reduction of NAD+. The protein encoded byiolG2, LcIDH2, has been purified to homogeneity, crystallized and cryoprotected for diffraction at 77 K. The crystals had a high mosaicity and poor processing statistics. Subsequent diffraction measurements were performed without cryoprotectant at room temperature. These crystals were radiation-resistant and a full diffraction data set was collected at room temperature to 1.6 Å resolution.
2015 ◽
Vol 71
(11)
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pp. 1448-1452
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2020 ◽
Vol 151
(9)
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pp. 1317-1328
Keyword(s):
2006 ◽
Vol 62
(2)
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pp. 197-204
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Keyword(s):