Effect of commercial probiotic (Protexin®) on growth, survival and microbial quality of shrimp (Litopenaeus vannamei)

2017 ◽  
Vol 47 (2) ◽  
pp. 204-216 ◽  
Author(s):  
Afshin Javadi ◽  
Seyed Amin Khatibi
Keyword(s):  
Survival Rate ◽  
Growth Performance ◽  
Litopenaeus Vannamei ◽  
Control Diet ◽  
Average Daily Gain ◽  
Bacterial Count ◽  
Total Bacterial Count ◽  
Microbial Quality ◽  
Content Type

Purpose The purpose of this study was to investigate the effect of a dietary probiotic on the growth performance and survival rate of Litopenaeus vannamei shrimp. Furthermore, the microbial quality of shrimp was evaluated. Design/methodology/approach Shrimp were divided into treatment and control groups (each group containing 45 shrimp). They were fed for four weeks with a control diet alone or supplemented with a commercial probiotic (Protexin®). At the end of the trial, they were assessed for survival rate, weight gain, average daily gain and specific growth rate. Samples of tail meat were also provided aseptically from peeled shrimp for bacteriological analysis including the count of Staphylococcus aureus, enterococci, Clostridium perfringens, fecal coliform, Salmonella, Escherichia coli, Listeria monocytogenes and total bacterial count. Findings The growth performance of the probiotic-treated group significantly (p < 0.05) increased at the end of the experimental period. However, no significant differences were observed for the survival rate between the groups (p > 0.05). The count of C. perfringens and the total bacterial count in shrimp supplemented with the probiotic were significantly lower than those of controls (p < 0.05). The count of coliforms and S. aureus was not significantly different between the groups (p > 0.05). Originality/value It could be concluded that the probiotic bacteria have the potential to stimulate the growth performance of L. vannamei. They can also be used for biological control of food-borne pathogens and improve the microbial quality and safety of shrimp at the farm level.

scholarly journals Rapid detection of microbial contamination in UHT milk: practical application in dairy industry 

2018 ◽  
Vol 36 (No. 5) ◽  
pp. 357-364
Author(s):  
Martina Kračmarová ◽  
Hana Stiborová ◽  
Šárka Horáčková ◽  
Kateřina Demnerová
Keyword(s):  
Microbial Contamination ◽  
False Negative ◽  
Bacterial Count ◽  
Total Bacterial Count ◽  
Microbial Quality ◽  
Uht Milk ◽  
Negative Results ◽  
False Negative Results ◽  
Dairy Plant

Microbial quality of ultra-high temperature (UHT) milk is usually ascertained by a total bacterial count (TBC) cultivation. But this is time consuming, so there is a tendency to search for faster and simpler methods. We compared three instruments, focusing on shortening the detection time and their suitability for practical use in dairy plants. Two of them, RapiScreen Dairy 1000 and Promilite III, detect microbial contamination by measuring adenosine triphosphate bioluminescence; the third, GreenLight, is based on oxygen consumption analysis. In the laboratory experiments, samples of UHT milk, were spiked with low concentration of microorganisms and then the level of microbial contamination was evaluated using the above-mentioned instruments together with cultivation method as a control. The instruments were also applied in a dairy plant to test 182 real samples. All investigated methods determined microbial quality faster than the TBC, but in some cases false positive and false negative results were obtained. Therefore, precise testing including optimizing pre-incubation time for bacteria enrichment is needed prior to industrial use. 

scholarly journals PSX-14 Effects of thyme or/and celery on feed utilization, growth performance and meat quality of Barki lambs

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 452-452
Author(s):  
Uchenna Anele ◽  
Mahmoud Shaaban ◽  
Ahmed Kholif ◽  
Ahmed Abd El Tawab ◽  
Mohamed Radwan ◽  
...  
Keyword(s):  
Growth Performance ◽  
Corn Stover ◽  
Control Diet ◽  
Average Daily Gain ◽  
Feed Utilization ◽  
Nutrient Digestibility ◽  
Control Treatment ◽  
Final Body Weight ◽  
Treatment Groups

Abstract The current study evaluated the effects of in-feed administration of thyme, celery or their mixture (as an alternative to salinomycin ionophore) on feed utilization and growth performance of Barki lambs. Fifty-five lambs weighing 18.5 ± 1.1 kg and about 90 ± 5 days old were stratified into 5 treatment groups and fed: (1) a control diet of concentrates and corn stover (60:40, on dry matter basis), or the control diet supplemented with (2) 15 g of thyme, (3) 15 g of celery, (4) 15 g of thyme and celery mix, or (5) 1 g of salinomycin/lamb daily for 288 days. Additives increased (P &lt; 0.05) both corn stover and total intakes, while thyme and thyme/celery mix increased nutrient digestibility (P &lt; 0.05) compared with the control treatment. Celery, thyme/celery mix and salinomycin treatments decreased serum cholesterol. Celery increased (P &lt; 0.05) final body weight, weight gain and average daily gain without affecting shrunk liveweight, hot carcass weight or dressing percent. Thyme/celery mix increased fat thickness (P &lt; 0.001), while thyme, celery and thyme/celery mix treatments increased (P = 0.001) the ribeye area. Salinomycin, celery and thyme treatments increased (P &lt; 0.05) the lean proportion and decreased the fat proportion. Thyme, celery and thyme/celery mix treatments decreased (P &lt; 0.001) carcass protein, while thyme and thyme/celery treatments increased (P = 0.001) carcass water holding capacity. Salinomycin, thyme and celery treatments decreased (P = 0.002) meat brightness score, while celery treatment increased (P &lt; 0.05) redness of meat, without affecting yellowness, chroma or hue of the meat. Results in the present showed that these natural additives are comparable to salinomycin but further studies are necessary to validate the results and their mechanism of action.

Effect of chia (Salvia hispanica L.) seeds incorporation on nutritional quality of muffin mix

2020 ◽  
Vol 50 (6) ◽  
pp. 1151-1161
Author(s):  
Vishakha Sharma ◽  
Renu Mogra ◽  
Azad Mordia
Keyword(s):  
Shelf Life ◽  
Wheat Flour ◽  
Control Sample ◽  
Value Added ◽  
Food Product ◽  
Bacterial Count ◽  
Total Bacterial Count ◽  
Content Type ◽  
Salvia Hispanica L

Purpose Study on chia seeds is receiving increasing interest because of their nutritional and health-promoting properties that have been recognized by their high content of antioxidants, proteins, minerals and dietary fibers. Hence, the purpose of this study is to develop a value-added food product using chia seeds and to assess the nutritional properties, shelf life and consumer acceptability of the developed product. Design/methodology/approach Six different formulations of muffin mix were prepared by incorporating chia seeds with wheat flour in various ratios and evaluated for sensory characteristics. The nutritional components and shelf lives of the developed products were also evaluated. The developed product was stored for three months to assess the shelf life. Findings Results indicated that muffins made with chia seeds can be added up to 20% in wheat flour without affecting the sensory scores (p = 0.0014). Regarding nutritional evaluation, the results revealed that 20% incorporation of chia seeds significantly increased the total antioxidant activity (p = 0.001), increased the crude protein (p = 0.0001), fat (p = 0.0001) and crude fiber (p = 0.001) contents and lowered the carbohydrate (p = 0.0001) content when compared to the control sample. Muffin mix made with chia seeds had significantly higher mineral content (calcium, phosphorus, zinc and iron) than did the control. The developed product had a shelf-life stability of three months in terms of moisture content, peroxide value and total bacterial count and was well accepted by consumers. Originality/value Intrinsic characteristics of chia seeds can improve the quality of muffins and bring health benefits to the consumers.

scholarly journals Milk Production, Marketing and Quality in Meta District of Eastern Hararghe Zone, Ethiopia

2019 ◽  
Vol 11 (5) ◽  
pp. 535
Author(s):  
Mitiku Eshetu Guya ◽  
Mulu Mamo Adugna ◽  
Yesihak Yusuf Mumed
Keyword(s):  
Chemical Composition ◽  
Milk Production ◽  
Bacterial Count ◽  
Quality Standard ◽  
Cow Milk ◽  
Total Bacterial Count ◽  
Microbial Quality ◽  
Milk Samples ◽  
Handling Practices

The study was conducted in Meta District of Eastern Hararge Zone Oromia Regional State, Ethiopia with the objective to assess milk production and marketing, and to determine chemical composition and microbial quality of raw cow milk. Three Rural Kebeles (RK) namely, Biftu Ganama, Oda Chafe and Waligalte Bali&rsquo;na were purposively selected based on dairy production potential and accessibility. A total of 132 respondents were randomly selected from farmers (122), consumers (6) and milk collection centers (4). Interview was made using semi-structured questionnaire to collect data on milk production, constraints, marketing, milk handling practices and utensils used as milk container. The result of the study indicated that, average daily milk production, milk for marketing and consumption were 1.96&plusmn;0.517 litters/day/cow, 68.9% and 31.1%, respectively. Majority of the respondents ranked feed shortage as the first constraint. Hand milking after calf suckling was practiced by all the farmers. Most of the respondents (56.6%) used a gourd container locally called &ldquo;qabe&rdquo; for milking, and plastic jerry can (81.96%) for milk delivery to milk collection centers. Significantly higher (P &lt; 0.05) figures were recorded for fat, protein, total solids, solids-not-fat and lactose contents at producers level than the consumers and collection centers. Total bacterial count (TBC), coliform count (CC) and yeast and mould count (YMC) of milk samples from milk collection centers were significantly higher (P &lt; 0.05) than milk samples from consumers and producers and did not meet quality standards set by Quality Standard Authority of Ethiopia. Therefore, we can concluded that the chemical composition of milk was satisfactory compared to the standard level, whereas the microbial quality of the milk was poor that suggests the need for better sanitary practices of milking environment and milk utensils.

scholarly journals Effects of Dietary Energy on Growth Performance, Rumen Fermentation and Bacterial Community, and Meat Quality of Holstein-Friesians Bulls Slaughtered at Different Ages

Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1123 ◽  
Author(s):  
Haibo Wang ◽  
Hang Li ◽  
Fei Wu ◽  
Xinjun Qiu ◽  
Zhantao Yu ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Growth Performance ◽  
Meat Quality ◽  
Energy Levels ◽  
Average Daily Gain ◽  
Rumen Fermentation ◽  
Dietary Energy ◽  
Different Ages ◽  
Slaughter Age

The objective of this study was to evaluate the effects of dietary energy levels on growth performance, rumen fermentation and bacterial community, and meat quality of Holstein-Friesians bulls slaughtered at different ages. Thirty-six Holstein-Friesians bulls (17 months of age) were divided into a 3 × 3 factorial experiment with three energy levels (LE, ME and HE; metabolizable energy is 10.12, 10.90 and 11.68 MJ/kg, respectively) of diets, and three slaughter ages (20, 23 and 26 months). Results indicated that bulls fed with ME and HE diets had higher dry matter intake, average daily gain, and dressing percentage at 23 or 26 months of age. The ME and HE diets also reduced bacterial diversity, altered relative abundances of bacteria and produced lower concentrations of acetate, but higher butyrate and valerate concentrations in rumen fluid. Increasing in dietary energy and slaughter age increased the intramuscular fat (IMF) and water holding capacity. In summary, Holstein-Friesians bulls fed with ME and HE diets, slaughtered at 23 and 26 months of age could be a good choice to produce beef with high IMF. Slaughter age may have less influence than dietary energy in altering fermentation by increasing amylolytic bacteria and decreasing cellulolytic bacteria, and thus, further affecting meat quality.

Effect of Yeast Type and Dose on Growth Performance and Nutrient Digestibility of Growing Rabbits Fed Maize or Barley

2021 ◽  
Author(s):  
Amr M.A. Elmasry ◽  
Luis A. Miranda ◽  
Germán Mendoza ◽  
Fernando X. Plata
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Body Weight ◽  
Growth Performance ◽  
Control Diet ◽  
Average Daily Gain ◽  
Nutrient Digestibility ◽  
Feed Conversion ◽  
Final Body Weight ◽  
Positive Effects ◽  
Yeast Concentration

Background: The effects of the probiotics vary with the doses and strains used for this reason, a study was conducted over 35 days to determine the effect of live yeast supplementation in the diet of growing rabbits on growth performance and apparent nutrient digestibility.Methods: A total of 60 New Zealand and California rabbits (5 weeks old, average body weight 0.881±0.015 kg) were housed in 30 cages. They were fed with a control diet based on alfalfa and maize or barley grains and supplemented with Procreatin®7 (Saccharomyces cerevisiae CNCM I 1077) or Biosaf® SC47 (Saccharomyces cerevisiae NCYC Sc47) at three doses, namely 0, 6.4, or 12.8 ×109 CFU per kg basal diet.Result: The higher yeast concentration had a positive effect (P less than 0.01) on final body weight (BW), average daily gain (ADG) and carcass weight (CW). However, there were no significant differences in dry matter intake. As a result of this, the feed conversion ratio (FCR) decreased (P less than 0.01). Significant increases (P less than 0.05) in nutrient digestibility (DM, OM, NDF and ADF) were observed with both grains. The use of yeast in maize and barley-based diets of rabbits has positive effects on growth performance and nutrient digestibility.

Effects of Quillaja saponin supplementation on growth performance, nutrient digestibility, fecal microbiota, and fecal gas emission in growing pigs

2021 ◽  
Author(s):  
De Xin Dang ◽  
In Ho Kim
Keyword(s):  
Growth Performance ◽  
Control Diet ◽  
Average Daily Gain ◽  
Dietary Supplementation ◽  
Fecal Microbiota ◽  
Nutrient Digestibility ◽  
Growing Pigs ◽  
Coliform Bacteria ◽  
Gas Emission ◽  
Quillaja Saponin

The purpose of this study was to evaluate the effects of dietary supplementation of Quillaja saponin (QS) on growth performance, nutrient digestibility, fecal microbiota, and fecal gas emission in growing pigs. A total of 50 crossbred growing pigs [(Yorkshire × Landrace) × Duroc] with an initial body weight of 23.83 ± 1.95 kg were randomly assigned to 1 of 2 treatments for a 56-day trial with 5 replicate pens per treatment and 5 pigs (2 barrows and 3 gilts) per pen. Dietary treatments including control diet and control diet supplemented with 200 mg/kg QS. The average daily gain was significantly increased during days 0-56, while the fecal ammonia emission on day 56 and fecal coliform bacteria counts on day 28 were significantly decreased in pigs fed with QS containing diet. However, dietary supplementation of QS had no significant effects on apparent total tract digestibility. In conclusion, dietary supplementation of 200 mg/kg QS had beneficial effects on growth performance, fecal microbiota, and fecal gas emission in growing pigs. Considering the carry-over effects, the adaption period should be at least 28 days when supplementing 200 mg/kg QS to the diet of growing pigs for improving the growth performance.

Evaluating the presence of human pathogens in commercially frozen, biologically appropriate raw pet food sold in Italy

2020 ◽  
Vol 187 (7) ◽  
pp. e50-e50
Author(s):  
Benedetta Bottari ◽  
Elena Bancalari ◽  
Annalisa Barera ◽  
Sergio Ghidini ◽  
Monica Gatti
Keyword(s):  
Feeding Strategy ◽  
Microbiological Quality ◽  
Bacterial Count ◽  
Total Bacterial Count ◽  
Limit Set ◽  
Human Pathogens ◽  
Pet Food ◽  
Pet Owners ◽  
Larger Sample

BackgroundBiologically appropriate raw food (BARF) diet is becoming more and more popular among pet owners in Europe. However, there are documented microbiological risks associated with raw feeding, and this study aimed to determine the presence of human pathogens in commercially frozen BARF products sold in Italy.MethodsSalmonella species, Escherichia coli O157:H7, Listeria monocytogenes and Campylobacter species were identified. The general microbiological quality of BARF products and hygiene were also evaluated. Sample size was limited and therefore the study may not be representative of a larger sample.ResultsNone of the tested samples showed total bacterial count (TBC) higher than the limit set to consider a sample unacceptable. However, 14 out of 21 samples showed TBC higher than the limit set to consider a sample marginally acceptable. A high percentage of samples were contaminated by the aforementioned pathogens, highlighting the need for pet owners to be aware of the risks of this feeding strategy both to themselves and to their pets.ConclusionsConsidering that BARF diet meals can be prepared at home using the hands, as well as tools and spaces that could be shared, guidelines on safer handling of these pet food products should be recommended by veterinarians and nutritionists.

scholarly journals Feeding Agroindustrial Byproducts to Light Lambs: Influence on Growth Performance, Diet Digestibility, Nitrogen Balance, Ruminal Fermentation, and Plasma Metabolites

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 600 ◽  
Author(s):  
Trinidad de Evan ◽  
Almudena Cabezas ◽  
Jesús de la Fuente ◽  
María Dolores Carro
Keyword(s):  
Growth Performance ◽  
Control Diet ◽  
Animal Health ◽  
Average Daily Gain ◽  
Plasma Concentrations ◽  
Barley Straw ◽  
Plasma Metabolites ◽  
Ruminal Fermentation ◽  
Feed Conversion ◽  
Homogeneous Groups

The objective of this study was to evaluate the effect of replacing cereals and protein concentrates in a high-cereal concentrate (control) for light lambs with corn distiller’s dried grains with solubles (DDGS; 18%), dried citrus pulp (DCP; 18%), and exhausted olive cake (EOC; 8%) in a byproduct (BYP) concentrate on growth performance, digestibility, ruminal fermentation, and plasma metabolites. Two homogeneous groups of Lacaune lambs (13.8 kg ± 0.25 kg) were fed one of each concentrates and barley straw ad libitum until reaching about 26 kg body weight. There were no differences between groups on feed intake, average daily gain, or feed conversion ratio, but the control diet had greater (p < 0.001) dry matter digestibility. Diet had no effect on post-mortem ruminal pH and total volatile fatty acid concentrations and profile, but NH3-N concentrations were lower (p = 0.003) for the BYP-fed group compared with the control one. However, plasma concentrations of amino acids, total proteins, urea, and hepatic enzymes were not affected by the diet. In conclusion, 44% of feed ingredients in the concentrate for light lambs can be replaced with a mixture of corn DDGS, DCP, and EOC without negatively affecting growing performance and animal health.

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