Phaleria Macrocarpa Crude Extract as Antidote for Cd (II) Contamination in Kidney Organs

2021 ◽  
Author(s):  
Ali Napiah Nasution ◽  
Rahmiana Zein ◽  
Refi Ikhtiari
Keyword(s):  
Crude Extract ◽  
Phaleria Macrocarpa

scholarly journals Biochemical Constituents of Phaleria macrocarpa (Leaf) Methanolic Extract Inhibit ROS Production in SH-SY5Y Cells Model

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Ibrahim Maina Hassan ◽  
Wan Norhamidah Wan Ibrahim ◽  
Ferdaus Binti Mohamat Yusuf ◽  
Siti Aqlima Ahmad ◽  
Syahida Ahmad
Keyword(s):  
Crude Extract ◽  
Mammalian Cells ◽  
Antioxidant Activities ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Inhibitory Effect ◽  
Natural Medicine ◽  
Cytotoxicity Studies ◽  
Phaleria Macrocarpa ◽  
Inhibitory Activities

Background. Reactive oxygen species generation in mammalian cells profoundly affects several critical cellular functions, and the lack of efficient cellular detoxification mechanisms which remove these radicals may lead to several human diseases. Several studies show that ROS is incriminated as destructive agents in the context of the nervous system especially with advance in age leading to neurodegeneration. Current treatments of this disease are not effective and result in several side effects. Thus, the search for alternative medicines is in high demand. Therefore, the aim of this study is to evaluate the reactive oxygen inhibitory effect of Phaleria macrocarpa 80% (leaf) extract. Methods. The leaf was extracted with 80% methanol. Cytotoxicity studies were carried out using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and ROS inhibitory activities were evaluated using dichlorofluorescein diacetate (DCF-DA) assay in the SH-SY5Y cells model. Results. The result revealed ROS inhibitory activities of the crude extract with highly significant differences at p < 0.001 between the group that were treated with crude extract only, the group treated with crude extract and exposed to H2O2, and the group exposed to H2O2 only as well as the group that were maintained in complete media. Bioactive compounds show the presence of vitexin and isovitexin following the HPLC method. Conclusion. High antioxidant activities and low toxicity effect of this crude revealed its high benefit to be used as natural medicine/supplements.

Genotoxicity evaluation in vitro by comet assay of compound and crude extract obtained from P. Pubescens Benth.

Planta Medica ◽  
2015 ◽  
Vol 81 (11) ◽  
Author(s):  
VHS Souza ◽  
A Paula OHohne ◽  
R Grando ◽  
N de Cassia de Almeida Queiroz ◽  
GM Pastore ◽  
...  
Keyword(s):  
Comet Assay ◽  
Crude Extract

Phytochemical screening for identification of bioactive compounds of Lantana camara Linn. (Verbenaceae) responsible for larvicidal action against Aedes triseriatus Say, 1823 (Diptera : Culicidae)

ENTOMON ◽  
2019 ◽  
Vol 44 (2) ◽  
pp. 117-126
Author(s):  
Rhitayu Chakraborti ◽  
Probir Kumar Bandyopadhyay
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Active Ingredient ◽  
Crude Extract ◽  
Ethyl Acetate Extract ◽  
Lantana Camara ◽  
Mosquito Larvae ◽  
Aedes Triseriatus ◽  
Phytochemical Screening ◽  
Lc50 Value

Study to assess the larvicidal property of Lantana camara leaves against Aedes triseriatus larvae found that the ethyl acetate extract had profound larvicidal action with the crude extract having a LC50 value of 409.831ppm. GC-MS analysis of the ethyl acetate extract confirmed the presence of twenty-one compounds out of which beta-caryophyllene covered the highest percentage of the chromatogram area. Further tests with beta-caryophyllene against the mosquito larvae proved it to be the active ingredient of L. Camara with a LC50 value of 104.243ppm.

Evaluation of a crude extract administration of Cnidoscolus chayamansa in normal and diabetic Wistar rats

2014 ◽  
Author(s):  
Francis Echeverria-Bobadilla ◽  
Andrea Ceballos-Arana ◽  
Daniel Sanchez-Ruz ◽  
Elda Pacheco-Pantoja
Keyword(s):  
Crude Extract ◽  
Wistar Rats ◽  
Cnidoscolus Chayamansa

Extraction and Phytochemicals Determination of Traditional Medicinal Plants for Anti-microbial Susceptibility Test

2020 ◽  
Vol 2020 ◽  
Author(s):  
Firew Admasu
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Traditional Medicine ◽  
Crude Extract ◽  
Susceptibility Test ◽  
Plant Sample ◽  
Traditional Medicinal Plants ◽  
Traditional Health Care ◽  
Susceptibility Tests

Abstract: The study were conducted at Dilla University, College of Natural Sciences, Biological Sciences laboratories. Background: Ethiopia is a country with many ethnic groups, cultures and beliefs which in turn have contributed to the high diversity of traditional health care knowledge and practices of traditional medicine from local growth plants, animals and minerals for various physical and mental disorders of human and livestock population that passed from generation to generation for centuries. Medicinal plants contributors to pharmaceutical, agricultural and food industries in the world. The use of medicinal plants in the industrialized societies has been traced to extraction and development of several drugs used in order to heel some diseases having inhibiting effect against pathogenic microorganism. Objective: The main objective of this study was Extraction and Phytochemicals determination of traditional medicinal plants for anti microbial susceptibility test. Methodology: The extraction and identification of some phytochemicals crude compound which used for antimicrobial susceptibility test from plant sample such as Ocimum lamiifolium (OL), Croton maerosth (Cm) and Ruta chalepesis (RC) were conducted. Plant samples are collected, powdered using mortal and pistil and extracted using ethanol and some susceptibility tests were performed to identify some phytochemicals compound. Result: The main result of Antimicrobial activity test showed that the crude extract of OL has the highest zone of inhibition. The highest yield of crude extract (38.21%) was obtained from Croton maerosth (CM) which followed by Ruta chalepesis (RC) (32.43%). However, the lowest yield (28.37%) was obtained from Oscpmum lamifolium (OL). Conclusion: Traditional Medicine is used by many people to managing numerous conditions; it’s accessible and effective on antimicrobial activity. Therefore, it plays a significant role by reducing life-threatening ailments of people and other animals.

Fractionation of Methanolic Crude Extract of Root of Senna siamea Decreases Antimicrobial Activity

2014 ◽  
Vol 1 (1) ◽  
pp. 37-42
Author(s):  
Mahmoud Jada ◽  
◽  
Wurochekke Usman ◽  
Bakodo Confidence
Keyword(s):  
Antimicrobial Activity ◽  
Crude Extract ◽  
Senna Siamea

Phytochemical analysis and salivary amylase inhibition activities of Carica papaya leaf and Garcinia mangostana pericarp extracts and partially purified fractions

2017 ◽  
Vol 6 (1) ◽  
pp. 34
Author(s):  
Michael Russelle Alvarez ◽  
Paolo Robert Bueno ◽  
Raymond Oliver Cruz ◽  
Richard Macapulay ◽  
Francis Jayson Vallesfin ◽  
...  
Keyword(s):  
Crude Extract ◽  
Carica Papaya ◽  
Uv Light ◽  
Digestive Enzyme ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Salivary Amylase ◽  
Garcinia Mangostana ◽  
Leaf Extracts

Plant-derived digestive enzyme inhibitors particularly those targeted to carbohydrate metabolism has been the focus of recent studies as natural supplements for weight control and diabetes. The present study explores the salivary amylase inhibition activity of Garcinia mangostana (Linn.) pericarp extracts and Carica papaya (Linn.) leaf extracts and fractions, as well as perform phytochemical screening and quantification, and thin layer – and high performance liquid chromatographic profiling. ­Results show that crude extracts and purified fractions were able to inhibit salivary amylase, with C. papaya fraction 1 being the most active at 30.89% inhibition. Phytochemical screening of all extracts tested ­positive for tannins, glycosides, phenolics, flavonoids and alkaloids. Quantification of phenolics showed that extracts contained high levels of phenolics, with C. papaya crude extract having the highest content with 219.0±12.7 mg GAE/g extract followed by G. mangostana crude extract with 247.1±18.0 mg GAE/g extract. Quantification of total flavonoids also showed C. papaya crude extract to contain the highest content with 55.12±0.679 mg QE/g extract. All extracts contained negligible alkaloid content, though. HPLC and TLC profiling showed several peaks and bands, when viewed in 210 nm and UV light, respectively. These results demonstrate in vitro the salivary amylase inhibitory activity of both plants and their potential as antidiabetic drug candidates; however, further studies need to be done, like isolation and structure elucidation of active components and toxicity assays. Keywords: Amylase inhibition, phytochemical quantification, Carica papaya, Garcinia mangostana

Valorization of the Pharmacological Potential of Phytochemical Compounds Contained in the Crude Extract of the Root of a Plant of Withania frutescens L

2019 ◽  
Author(s):  
A. EL Moussaoui ◽  
F. Jawhari ◽  
K. EL Ouahdani ◽  
I. Es-Safi ◽  
D. Bousta ◽  
...  
Keyword(s):  
Crude Extract ◽  
Control Group ◽  
Root Extract ◽  
Nacl Solution ◽  
Phytochemical Compounds ◽  
Anti Inflammatory ◽  
Pharmacological Potential

Our present study focuses on the evaluation of the analgesic, anti-inflammatory and healing activity of Withania frutescens L. The anti-inflammatory result has an inhibition percentage of 78.87% ± 7.08 at 450 mg/kg and 75.14% ± 6.39 at 400 mg/kg and 89.75% ± 3.44 for diclofenac (1%). When applied locally, the 10% cream has an inflammation inhibition of 96.87% ± 5.85 and 76.14% ± 7.88 for the 5% cream with 89.87 ± 6.20 of reference (Indomethacin). The abdominal contractions of rats treated with the root extract are significantly lower than those of the control group that received only physiological NaCl solution, with 41.20 ± 2.30 for the extract and 82.20 ± 5.04 for NaCl and 53.40 ± 4.94 for the reference. The healing activity of the studied extract records a percentage of contraction of about 93.20% ± 3.36 (Extract 10%), 84.50% ± 3.84 (Extract 5%), 48.47% ± 2.15 (control) and 81.88 ± 2.24 for the reference.

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