Extension of a nutrient demand model for shrimp to include the ideal protein concept to predict amino acid demands

2021 ◽  
Author(s):  
Brett D. Glencross
Author(s):  
T. C. Wang ◽  
M. F. Fuller

An animal's rate of protein accretion (or nitrogen retention) is determined by the most deficient (or first limiting) amino acid in the diet, in a perfectly balanced (or ideal) protein all essential amino acids and the sum of the non-essential amino acids are equally limiting. If a dietary protein had any amino acids in excess of the ideal pattern, then the removal of any of the excess should not affect nitrogen retention. This principle was used to determine the ideal dietary amino acid pattern for growing pigs.Three nitrogen balance trials were carried out with a total of 64 gilts of weight from 30 to 55 kg. Casein and a mixture of amino acids were used in the semi-purified diets. The animals were given the diets at the rate of 93 g/kg BW0.75/d in three equal meals mixed with 0.3 L water. The feeding times were 08.30, 12.30 and 17.30. All pigs received their experimental diets for 7 days, made up of 3 days preliminary and a 4-day collection, except that the preliminary period before the first collection in the first period was 7 days. Before the first collection bladder catheters were introduced.


2017 ◽  
Vol 43 (5) ◽  
pp. 888-894
Author(s):  
Wilson Massamitu Furuya ◽  
Mariana Michelato ◽  
Ana Lúcia Salaro ◽  
Thais Pereira da Cruz ◽  
Valéria Rossetto Barriviera-Furuya

Colliroja, Astyanax fasciatus, is a new aquaculture species, and information on its dietary essential amino acid requirements is lacking. The whole body composition of 120 farmed fish (16.2 ± 8.8 g) was determined to estimate the dietary essential amino acid requirement based on the ideal protein concept ((each essential amino acid/lysine) ×100), and the findings were correlated to the whole body essential amino acid content of Nile tilapia Oreochromis niloticus. The dietary essential amino acids, including cysteine and tyrosine, accounted for 5.46, 4.62, 1.16, 3.28, 5.63, 2.01, 2.59, 2.84, 4.66, 3.39, 0.65, and 3.51% of the total protein for lysine, arginine, histidine, isoleucine, leucine, methionine, methionine+tyrosine, phenylalanine, phenylalanine+tyrosine, threonine, tryptophan, and valine, respectively. There were positive linear and high correlations (r = 0.971) between the whole body amino acid profiles of colliroja and Nile tilapia. Thus, the whole body amino acid profile of colliroja might be used to estimate accurately the essential amino acid requirement.


1995 ◽  
Vol 1995 ◽  
pp. 38-38
Author(s):  
P.H. Simmins ◽  
J.C. Bodin ◽  
A. Kies ◽  
P.E.V. Williams

The efficient production of the weaner pig requires accurate knowledge of its amino acid requirements. This is particularly relevant today as it is increasingly important to reduce nitrogenous waste from pigs. During the past decade formulations for feeds were based on the ideal protein profile of the lean tissue of pigs in which the sulphur amino acid requirements were assessed as the combination of total sulphur amino acids rather than through their individual amino acids, methionine and cystine (Agricultural Research Council, 1981). Today, this concept is challenged. The effect of the changing cystine demand in the weaner pig on the total ileal digestible sulphur amino acid (IDSAA) requirements and hence methionine levels requires study. The objective of this experiment was to understand the requirements and relationship between true ileal digestible cystine and methionine to lysine ratio in the ideal protein content of food to achieve efficient production in the young pig.


2020 ◽  
Vol 18 (5) ◽  
pp. 54-65
Author(s):  
A.T. Vasyukova ◽  
◽  
D.A. Tikhonov ◽  
A.V. Moshkin ◽  
I.A. Bogonosova ◽  
...  

The article presents a comparative characteristic of the amino acid composition of the «ideal» protein and the developed formulation of combined products. The results of calculation of the amino acid score (AAS) of the essential amino acids in the novel protein product are given. AAS is within the range from 105 to 143%. The developed formulation of a combined product has an insignificantly limited amount of valine (98%). Based on AAS, the difference coefficients of the examined amino acids were calculated (from –0.2 to 4.36%) and the biological value of each of the components of the formulation was determined. The nonessential amino acid composition of the «ideal» protein, raw materials of vegetable and animal origin and products produced according to the developed formulation are considered. The formulation of the novel combined product has been characterised as a full and balanced amino acid composition, suitable for nutrition, containing all the amino acids necessary for protein synthesis. The maximum AAS has been noted in steamed meatballs with beef, mutton and cabbage, which by 7.61% exceeds the AAS of steamed meatballs with beef and mutton and by 9.38% – the AAS of steamed meatballs with beef. The examined product has a high biological value – from 95 to 99%. The developed mathematical model is applicable to specification of the formulation under more specific conditions, for instance, for a particular age category. The novelty of the model consists in its priority with respect to the organoleptic properties and consumer preferences of the developed formulation. Key words: combined products, simplex method, amino acid score, formulation composition, protein


2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4531
Author(s):  
Charlyan De Sousa Lima ◽  
Marcos Antonio Delmondes Bomfim ◽  
Jefferson Costa de Siqueira ◽  
Eduardo Arruda Teixeira Lanna ◽  
Felipe Barbosa Ribeiro ◽  
...  

<p>The aim of this study was to evaluate the effect that reduced crude protein (CP) diets with amino acid  supplementation, based on the ideal protein concept, have on the performance of tambaqui fingerlings. In total, 750 fingerlings with an initial weight of 0.44 ± 0.14 g, were used in a completely randomized design, with six treatments, five replications, and 25 fish per experimental unit. Treatments consisted of six isocaloric, isocalcic, isophosphoric, and isolysinic diets containing 32.0, 30.5, 29.0, 27.5, 26.0, and 24.5% CP. Performance parameters, carcass yield, body composition (body humidity, protein, and fat content), and nitrogen retention efficiency (NRE) of the fish were evaluated. When the CP content was reduced to 27.5%, there was an improvement in weight gain, specific growth rate, feed conversion, and protein efficiency rate. Feed intake was higher in fish fed diets containing 26% CP, followed by those fed diets containing 29 and 27.5% CP; diets containing other levels of CP did not differ among themselves. CP intake was reduced by the use of diets containing 24% CP, and the carcass yield was not influenced by the treatments. Reduced CP had no influence on body composition. NRE was higher in response to diets containing 26.0 and 24.5% CP. Reducing dietary CP content, from 32.0 to 24.5%, based on the ideal protein concept, is possible for tambaqui fingerlings, and a decrease of up to 24.5% enhances the fish performance.</p>


2020 ◽  
Vol 3 (5) ◽  
pp. 116-121
Author(s):  
Hébert Fernandes de Brito ◽  
◽  
Wagner Azis Garcia de Araújo ◽  
Luciano Xavier dos Santos ◽  
Herbeth Leite Colares ◽  
...  

Nutrition is responsible for most of the production costs and the formulation of balanced diets is of paramount importance to provide better productive performance and greater economic efficiency. Thus, the emergence of industrial amino acids allowed the elaboration of diets based on the ideal protein, providing an amino acid profile closer to the animals' needs, improving the use of nutrients and decreasing the excretion of dietary nitrogen in the environment. In view of the benefits presented using the ideal protein concept in the formulation of diets, this review aimed to show its application in poultry and swine nutrition


2021 ◽  
Vol 9 (10) ◽  
pp. 94-107
Author(s):  
Shaimaa R. Anwar ◽  
Ahmed A. Al-Sagan ◽  
El- Bannaa R. A ◽  
Melegy T. M

A comparative study was conducted to determine the impacts of crude protein (CP) reductions in laying hen diets. During phase one of egg production, 270 laying hens were randomly assigned into six dietary treatments, which were replicated three times using a 2 × 3 factorial experiment. Reduced CP and metabolizable energy (ME) diets were formulated based on the ideal amino acid profile concept and supplemented with crystalline amino acids. The control treatment received standard commercial diets containing 18.8% CP and 2,725 kcal/kg ME. Average weight gain, hen day egg production percentage, egg weight, egg mass, and other selected egg quality parameters were recorded. Data were statistically analyzed. Feed consumption and the feed conversion ratio were improved in birds fed reduced CP diets supplemented with crystalline amino acids. The dietary CP level significantly influenced shell thickness (ST), while the dietary ME level had no effect. A highly significant interaction was observed between CP percent and the Haugh unit (HU).  Results indicate that the “ideal protein concept” may be followed as an economically feasible option for laying hens since it optimizes the dietary amino acid profiles and ME levels, and have a positive effect on hen growth, egg quality and environmental pollution.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 12-13
Author(s):  
So-Young Kim

Abstract Global Amino acids market is estimated as $15billion in 2020 and 60% of those volume is used for feed additives. Amino acids are converted to protein efficiently and have a benefit of lowering feeding cost and reducing the nitrogen content of the waste output compared to intact protein diet. Most amino acids are produced using microbial fermentation. Traditionally, amino acids used for feed additives are Lysine, threonine, tryptophan and methionine. Among them methionine was produced only by chemical process, but now methionine can also be produced by microbial fermentation, which can enlarge the choice of methionine from DL-form to L-form. Use of L-form methionine as feed additives reveals new nutritional value of L-methionine compared to DL-methionine. Recently, valine, arginine, isoleucine, and histidine are also included in feeds as functional nutrients thanks to their commercial availability and lower price. Technical progress in microbiology and bioengineering has been made more diverse amino acid to be used as feed additives. Then what would be the next amino acid as feed additivities based on microbial point of view? Leucine, glutamine, tyrosine and phenylalanine could be next possible amino acids based on metabolic pathways of microorganism. These amino acids share the pathways with former feed amino acids such as valine or tryptophan, which means that these can be produced with way that is more economical. In addition, these amino acids could be produced as mixed forms like glutamic acid/glutamine mixture, Tryptophan/tyrosine/phenylalanine mixture, or BCAA forms with cheaper price. Dried fermentation product such as lysine sulfate made by drying of microbial culture broth, could be one of the option for the combination of several new amino acids. Crude protein reduction in feed is one of the big nutritional trend to reach the ideal protein diet and environmental clean situation. Considering the use of new synthetic amino acids in feeds would be a potential way to realize the ideal protein diet.


2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4531
Author(s):  
Charlyan De Sousa Lima ◽  
Marcos Antonio Delmondes Bomfim ◽  
Jefferson Costa de Siqueira ◽  
Eduardo Arruda Teixeira Lanna ◽  
Felipe Barbosa Ribeiro ◽  
...  

The aim of this study was to evaluate the effect that reduced crude protein (CP) diets with amino acid supplementation, based on the ideal protein concept, have on the performance of tambaqui fingerlings. In total, 750 fingerlings with an initial weight of 0.44 ± 0.14 g, were used in a completely randomized design, with six treatments, five replications, and 25 fish per experimental unit. Treatments consisted of six isocaloric, isocalcic, isophosphoric, and isolysinic diets containing 32.0, 30.5, 29.0, 27.5, 26.0, and 24.5% CP. Performance parameters, carcass yield, body composition (body humidity, protein, and fat content), and nitrogen retention efficiency (NRE) of the fish were evaluated. When the CP content was reduced to 27.5%, there was an improvement in weight gain, specific growth rate, feed conversion, and protein efficiency rate. Feed intake was higher in fish fed diets containing 26% CP, followed by those fed diets containing 29 and 27.5% CP; diets containing other levels of CP did not differ among themselves. CP intake was reduced by the use of diets containing 24% CP, and the carcass yield was not influenced by the treatments. Reduced CP had no influence on body composition. NRE was higher in response to diets containing 26.0 and 24.5% CP. Reducing dietary CP content, from 32.0 to 24.5%, based on the ideal protein concept, is possible for tambaqui fingerlings, and a decrease of up to 24.5% enhances the fish performance.


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