Phenolic Content in Male and Female Carica papaya: A Possible Physiological Marker for Sex Identification of Vegetative Seedlings

1975 ◽  
Vol 33 (1) ◽  
pp. 104-107 ◽  
Author(s):  
K. K. JINDAL ◽  
R. N. SINGH
Keyword(s):  
Carica Papaya ◽  
Phenolic Content ◽  
Sex Identification ◽  
Male And Female

A rapid method for sex identification in Cannabis sativa using High Resolution Melt (HRM) analysis.

Botany ◽  
2021 ◽  
Author(s):  
Erin Jacqueline Gilchrist ◽  
Daniela Hegebarth ◽  
Shumin Wang ◽  
Teagen D. Quilichini ◽  
Jason Sawler ◽  
...  
Keyword(s):  
High Resolution ◽  
Rapid Method ◽  
X Chromosome ◽  
Cannabis Sativa ◽  
Sex Identification ◽  
Male Plant ◽  
High Resolution Melt ◽  
Male And Female ◽  
High Resolution Melt Analysis ◽  
Plant Sex

We report the identification of two SNPs in Cannabis sativa that are associated with female and male plant sex phenotypes, and are located on the top arm of the X chromosome. High Resolution Melt analysis was used to develop and validate a novel, rapid method for sex identification in medical/recreational cannabis as well as in hemp. This method can distinguish between dioecious male (XY) and dioecious female (XX) cannabis plants with 100% accuracy, and can also be used to differentiate between male and female Humulus lupulus (hop) plants.

scholarly journals Perbedaan Ukuran Gigi Molar Pertama Maksila dan Kaninus Mandibula Permanen Antara Mahasiswa Laki-laki dan Perempuan di FKG Universitas Jember

2018 ◽  
Vol 6 (2) ◽  
pp. 358
Author(s):  
Najla Irhamni Phasa ◽  
Dwi Kartika Apriyono ◽  
Masniari Novita
Keyword(s):  
Female Student ◽  
Sex Identification ◽  
T Test ◽  
Tooth Size ◽  
Mass Disaster ◽  
Male And Female ◽  
Digital Caliper

Mass disaster often happened in Indonesia. Sex identification is one of the important things for determining the identity of the victims. Sex can be identified based on the size of the teeth.  The study determine the differences of maxillary first molar and mandibular caninus tooth size between male and female student in Faculty of Dentistry University of Jember. The sample of this study consisted of 34 tooth models of female and 6 tooth models of male that measured in mesiodistal and bucolingual width of maxillary first molar and mandibular caninus with a digital caliper. Data were analyzed using Independent T-test. There were significant differences (p ≤ 0,05) of maxillary first molar and mandibular caninus size between male and female. Mesiodistal and bucolingual width of maxillary first molar respectively are 10.64 mm, 11.90 mm in males, and 10.16 mm, 11.16 mm in females. The mesiodistal and bucolingual width of mandibular caninus are 7.13 mm, 7.85 mm in males, and 6.66 mm, 7.08 mm in females. It can be concluded that there was a difference in size of maxillary first molar and mandibular caninus between male and female. Keywords: sex identification, masiodistal width, bucolingual width, maxillary first molar, mandibular caninus

scholarly journals Comparative proteomics analysis reveals differentially accumulated proteins associated with male and female A. chinensis var. chinensis bud development

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Yu Zhang ◽  
Yuexing Wang ◽  
Wanying Zhou ◽  
Shimao Zheng ◽  
Wenhui Zhang
Keyword(s):  
Ribosomal Protein ◽  
Interaction Analysis ◽  
Economic Benefits ◽  
Sex Identification ◽  
Raw Material ◽  
Regulation Mechanism ◽  
Label Free ◽  
Flower Bud ◽  
Male And Female ◽  
Bud Development

Abstract Background Kiwifruit (Actinidia chinensis var. Chinensis) is abundant with vitamin C and is a rapidly developing crop in China, New Zealand, and other countries. It has been widely used as a raw material for food and kiwifruit wine. Among these, A. chinensis var. chinensis and A. chinensis var. deliciosa are the most valuable kiwifruit in production. Kiwifruit is a typical dioecious plant and its female and male plants have different economic values. Therefore, sex identification, especially at the seedling stage, has important implications for the scientific planning of its production and economic benefits. However, the kiwifruit sex regulation mechanism is very complex and molecular studies are in the initial stages. Currently, there is not a universal and effective sex identification method for A. chinensis. Methods In this study, we used a label-free quantitative proteomics approach to investigate differentially accumulated proteins, including their presence/absence and significantly different levels of abundances during A. chinensis var. chinensis male and female flower bud development. Results A total of 6485 proteins were identified, among which, 203 were identified in male buds, which were mainly associated with phenylalanine metabolism, tyrosine metabolism, and plant hormone signal transduction. In female buds, 241 were identified, which were mainly associated with the ErbB signaling pathway, growth hormone synthesis, secretion and action, and mRNA surveillance pathway. A total of 373 proteins were significantly differentially accumulated proteins (fold change > 2; P < 0.05), of which, 168 were upregulated and 205 were downregulated. Significant differences between proteins involved 13 signaling pathways, most of which were involved in flavonoid biosynthesis, phenylpropanoid biosynthesis, and starch and sucrose metabolism. Protein interaction analysis showed that enriched protein nodes included cell division cycle 5-like protein, 40S ribosomal protein S8, ribosomal protein, and 40S ribosomal protein like, which interact with 35, 25, 22, and 22 proteins, respectively. Conclusions This study provide valuable information for cloning key genes that control sex traits and functionally analyze their roles, which lay a foundation to the development of molecular markers for male and female kiwifruit identification.

scholarly journals Changes in Total Phenolic and Protein Contents during Production of Protein Isolates from Carica papaya (Pawpaw) Seeds

2016 ◽  
Vol 4 (11) ◽  
pp. 1006 ◽  
Author(s):  
Oseni Kadiri ◽  
Saka O Gbadamosi ◽  
Babatunde Olawoye ◽  
Charles T Akanbi
Keyword(s):  
Protein Content ◽  
Total Phenolic Content ◽  
Carica Papaya ◽  
Phenolic Content ◽  
Production Method ◽  
Protein Isolate ◽  
Total Phenolic ◽  
Isoelectric Precipitation ◽  
Protein Isolates ◽  
Gallic Acid Equivalent

The effect of different processing stages- defatting, solubilization, isoelectric precipitation, neutralisation and lyophilisation on the total phenolic content and protein content of Carica papaya (pawpaw) seeds (CPS) full-fat flour (CFF), defatted flour (CDF), protein concentrates (CPC) and protein isolates (CPI) was studied. The folin-ciocalteu procedure was used to assess the total phenolic concentrations of the Carica papaya products and results were expressed as gallic acid equivalent (GAE). Optimal protein content was estimated at pH 10 for all samples. Higher levels of polyphenols were extracted using water-methanol (20/80) mixture compared to 100% methanol. Total phenolic content (TPC) was demonstrated in a trend of CDF > CPC > CFF > CPI with CDF having the highest TPC. The finding suggested a more economical protein isolate production method. The study concludes that the processing of CPS into the various products (CFF, CDF, CPC & CPI) improves its protein and polyphenol content.

scholarly journals PHENOLIC CONTENT, ANTIOXIDANT ACTIVITY AND BIODIVERSITY CHANGES DURING SPONTANEOUS FERMENTATION OF CARICA PAPAYA LEAF

2019 ◽  
Vol 82 (1) ◽  
Author(s):  
Mohamad Sufian So'aib ◽  
Ku Halim Ku Hamid ◽  
Jailani Salihon ◽  
Huey Ling Tan
Keyword(s):  
Antioxidant Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Carica Papaya ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Spontaneous Fermentation ◽  
Fermentation Time

The spontaneous fermentation was carried out on Carica papaya leaf (CPL) in view of its potential improvement on antioxidant functionality and cultivation of lactic acid bacteria. The effect of the spontaneous fermentation on the total phenolic content and antioxidant activity of CPL, as well as biodiversity profiling were evaluated in this study. Total phenolic content and antioxidant capacity of the fermented CPL were 31.14 mg GAE g-1 and 405.8 mM TE g-1 respectively, higher than the unfermented CPL (5.71 mg GAE/g and 130.5 mM TE g-1) respectively. Microbial community was predominantly lactic acid bacteria (LAB) and yeasts, both populated at 104 to 108 CFU/mL during most part of the fermentation. Presumptive Enterobacteriaceae showed up briefly at the onset of the fermentation before disappearing. PCR-DGGE fingerprinting revealed Lactobacillus plantarum (Lb. plantarum) as the sole dominant bacterial species. More diverse yeasts community was detected by PCR-DGGE where succession of Zygosaccharomyces, Saccharomyces, Candida and Aspergillus genera were detected along fermentation time. Spontaneous fermentation successfully enhanced the total phenolic content and antioxidant capacity of the CPL. The cultivation of lactic acid bacteria was indicated by the presence of Lb. plantarum, whereas the disappearance of Enterobacteriaceae conferred a safe consumption of the fermented CPL.

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