HPLC ANALYSIS OF n-3 AND n-6 FATTY ACID LEVELS IN RAT SERUM AFTER CHRONIC TREATMENT WITH DIETETIC OILS

2009 ◽  
Vol 16 (4) ◽  
pp. 422-435 ◽  
Author(s):  
CECILIA RUSTICHELLI ◽  
ROSSELLA AVALLONE ◽  
ENRICO CAMPIOLI ◽  
DANIELA BRAGHIROLI ◽  
CARLO PARENTI ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Hplc Analysis ◽  
Chronic Treatment ◽  
Rat Serum

scholarly journals Oils’ Impact on Comprehensive Fatty Acid Analysis and Their Metabolites in Rats

Nutrients ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1232
Author(s):  
Agnieszka Stawarska ◽  
Małgorzata Jelińska ◽  
Julia Czaja ◽  
Ewelina Pacześniak ◽  
Barbara Bobrowska-Korczak
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Olive Oil ◽  
Edible Oils ◽  
Solid Phase ◽  
Linseed Oil ◽  
Standard Diet ◽  
Sprague Dawley ◽  
Rat Serum ◽  
Serum Fatty Acid

Fatty acids, especially polyunsaturated, and their metabolites (eicosanoids) play many pivotal roles in human body, influencing various physiological and pathological processes. The aim of the study was to evaluate the effect of supplementation with edible oils diverse in terms of fatty acid composition on fatty acid contents, activities of converting their enzymes, and on lipoxygenase metabolites of arachidonic and linoleic acids (eicosanoids) in rat serum. Female Sprague-Dawley rats divided into seven groups were used in the study. Animals from six groups were fed one of oils daily (carotino oil, made up by combining of red palm oil and canola oil, linseed oil, olive oil, rice oil, sesame oil, or sunflower oil). One group received a standard diet only. Fatty acids were determined using gas chromatography with flame ionization detection. Eicosanoids—hydroxyeicosatetraenoic (HETE) and hydroxyoctadecadienoic acids (HODE) were extracted using a solid-phase extraction method and analyzed with HPLC. Vegetable oils given daily to rats caused significant changes in serum fatty acid profile and eicosanoid concentrations. Significant differences were also found in desaturases’ activity, with the linseed and olive oil supplemented groups characterized by the highest D6D and D5D activity. These findings may play a significant role in various pathological states.

scholarly journals Content of Lipophilic Compounds and HPLC Analysis of Fatty Acid Components in Wine Vinegar

2005 ◽  
Vol 54 (10) ◽  
pp. 559-563 ◽  
Author(s):  
Masahiro FUJIMORI ◽  
Keita YUNOKI ◽  
Toshimitsu SHIMIZU ◽  
Tsutomu MASUDA ◽  
Yoshinori TSUKAMOTO ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Hplc Analysis ◽  
Lipophilic Compounds ◽  
Wine Vinegar

Potato tubers exhibit both homolytic and heterolytic hydroperoxide fatty acid-cleaving activities

2000 ◽  
Vol 28 (6) ◽  
pp. 853-855 ◽  
Author(s):  
M.-L Fauconnier ◽  
J. Delcarte ◽  
P. Hoyaux ◽  
P. du Jardin ◽  
M. Marlier
Keyword(s):  
Fatty Acid ◽  
Potato Tuber ◽  
Linolenic Acid ◽  
Hplc Analysis ◽  
Potato Tubers ◽  
Almost All ◽  
Fatty Acid Hydroperoxides ◽  
Tuber Extract

The action of a crude potato-tuber extract on 9- and 13-hydroperoxides of linoleic and linolenic acids was investigated. HPLC analysis revealed that 50% of the 9-hydroperoxide isomers and almost all the 13-hydroperoxide isomers were rapidly enzymically metabolized. No degradation of fatty acid hydroperoxides was observed with a thermally denatured enzymic extract. GC-MS identification of the volatiles formed by the reaction revealed that no volatiles were detected from the 9-hydroperoxide isomers, whereas 13-hydroperoxide of linolenic acid was cleaved into (Z)-3-hexenal, pentenols or dimers of pentene.

Chemiluminescence-HPLC Analysis of Fatty Acid Hydroperoxide Isomers

1993 ◽  
Vol 57 (9) ◽  
pp. 1575-1576 ◽  
Author(s):  
Yasuo Kondo ◽  
Yoshiyuki Kawai ◽  
Teruo Miyazawa ◽  
Junya Mizutani
Keyword(s):  
Fatty Acid ◽  
Hplc Analysis ◽  
Fatty Acid Hydroperoxide ◽  
Acid Hydroperoxide

ON RAT SERUM LIPASE

1952 ◽  
Vol 30 (6) ◽  
pp. 453-456
Author(s):  
Jack D. Taylor ◽  
Jules Tuba
Keyword(s):  
Fatty Acid ◽  
Corn Oil ◽  
Linseed Oil ◽  
Fatty Acid Content ◽  
Dietary Fats ◽  
Male Rats ◽  
Rat Serum ◽  
Serum Lipase ◽  
Dietary Concentration ◽  
Test Level

Several dietary fats were tested for their effect on the serum tributyrinase activity of adult male rats. Enzyme levels were significantly higher in rats subsisting on diets containing 23% of any of the fats which were tested, when compared with values for animals which were given a dietary concentration of 8% of the same fat. At the 8% level there were significant differences in tributyrinase activity which appeared to be associated with the fatty acid composition of the lipid. In order of decreasing effect on the serum enzyme at the 8% dietary test level the fats are listed as follows: Crisco, butter, cottonseed oil, linseed oil, corn oil, and olive oil. At the 23% dietary level there are fewer significant effects on the enzyme which could be due to the fatty acid content of the various lipids.

Polyunsaturated fatty acid levels in rat tissues after chronic treatment with dietetic oils

2011 ◽  
Vol 92 (2) ◽  
pp. 239-245 ◽  
Author(s):  
Cecilia Rustichelli ◽  
Rossella Avallone ◽  
Enrico Campioli ◽  
Daniela Braghiroli ◽  
Mario Baraldi
Keyword(s):  
Fatty Acid ◽  
Polyunsaturated Fatty Acid ◽  
Chronic Treatment ◽  
Rat Tissues

Properties of phospholipase A2 isolated from rat serum

1991 ◽  
Vol 69 (5-6) ◽  
pp. 358-365 ◽  
Author(s):  
R. R. Baker ◽  
H.-y. Chang
Keyword(s):  
Fatty Acid ◽  
Molecular Mass ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Phospholipase A ◽  
Purification Step ◽  
Ph Optimum ◽  
Rat Serum ◽  
Absolute Requirement ◽  
Hydrolysis Of

Phospholipase A2 was extensively purified (1300- to 1400-fold) from rat serum using Sephadex G-100 chromatography. It eluted at a position corresponding to a molecular mass of about 15 kDa. This one purification step gave two bands on sodium dodecyl sulfate – polyacrylamide gel electrophoresis. The faster component had a molecular mass of 16 kDa and the slower band likely contained an aggregate of the faster component. Activity was associated with protein bands on nondenaturing gels. Enzyme activity was assessed using phosphatidylcholine or phosphatidylethanol-amine labelled at sn position 2 with radioactive arachidonate. Phosphatidylethanolamine gave higher specific activities than phosphatidylcholine. The enzyme has an absolute requirement for Ca2+ and a pH optimum at 7.4. This pH optimum was more prominent for phosphatidylethanolamine. Activity was inhibited by oleate or arachidonate when phosphatidylcholine was used as substrate, but added free fatty acid did not significantly affect the hydrolysis of phosphatidylethanolamine. Addition of bovine serum albumin (fatty acid free) to assays increased the rate of release of arachidonate from phosphatidylcholine, but not from phosphatidylethanolamine. Phospholipase A2 is present in serum likely as a consequence of blood coagulation and may release fatty acids from cellular membranes following hemorrhage.Key words: phospholipase A2, phosphatidylcholine, phosphatidylethanolamine, rat serum.

THE EFFECT OF FREE FATTY ACIDS ON RESIN UPTAKE OF TRI-IODOTHYRONINE FROM HUMAN, RAT, RABBIT AND GUINEA-PIG SERUM AND HUMAN SERUM ALBUMIN

1969 ◽  
Vol 45 (4) ◽  
pp. 489-493 ◽  
Author(s):  
P. W. NATHANIELSZ
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Human Serum Albumin ◽  
Guinea Pig ◽  
Serum Albumin ◽  
Free Fatty Acids ◽  
Human Serum ◽  
Rat Serum ◽  
Pig Serum

SUMMARY Recently changes in plasma free fatty acids have been suggested as a possible regulator of the levels of free thyroxine in the plasma. Oleic acid has been shown to displace tri-iodothyronine from human serum, human serum albumin, rat serum, rabbit serum and guinea-pig serum. The extent of the displacement, much greater from human serum albumin than from whole serum, suggests that free fatty acid does not affect the globulin binding site. It would also appear that, in the rat, all the binding sites are sensitive to free fatty acids and hence there is probably only albumin binding in this species. The results with rabbit and guinea-pig serum were intermediate to those with human and rat serum. A significant rise in resin uptake of tri-iodothyronine in vitro occurred with an increase of free fatty acid level of 0·5 m-equiv./l., well within the physiological range.

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