ON RAT SERUM LIPASE

1952 ◽  
Vol 30 (6) ◽  
pp. 453-456
Author(s):  
Jack D. Taylor ◽  
Jules Tuba
Keyword(s):  
Fatty Acid ◽  
Corn Oil ◽  
Linseed Oil ◽  
Fatty Acid Content ◽  
Dietary Fats ◽  
Male Rats ◽  
Rat Serum ◽  
Serum Lipase ◽  
Dietary Concentration ◽  
Test Level

Several dietary fats were tested for their effect on the serum tributyrinase activity of adult male rats. Enzyme levels were significantly higher in rats subsisting on diets containing 23% of any of the fats which were tested, when compared with values for animals which were given a dietary concentration of 8% of the same fat. At the 8% level there were significant differences in tributyrinase activity which appeared to be associated with the fatty acid composition of the lipid. In order of decreasing effect on the serum enzyme at the 8% dietary test level the fats are listed as follows: Crisco, butter, cottonseed oil, linseed oil, corn oil, and olive oil. At the 23% dietary level there are fewer significant effects on the enzyme which could be due to the fatty acid content of the various lipids.

Influence of Melatonin and Linseed Oil on the State of Lipid Metabolism at Desynchronosis in Rats

2021 ◽  
pp. 58-68
Author(s):  
Irina S. Sobolevskaya ◽  
◽  
Oleg D. Myadelets ◽  
Natalʼya N. Yarotskaya
Keyword(s):  
Lipid Metabolism ◽  
Linseed Oil ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Atherogenic Index ◽  
Male Rats ◽  
Serum Concentrations ◽  
Rat Serum ◽  
Lighting Conditions

The purpose of this study was to substantiate the possibility of correcting lipid metabolism changes at dark deprivation using linseed oil, melatonin, and their combination. Materials and methods. The experiment involved 130 white outbred male rats with a body weight of 170–220 g. The animals were divided into 5 groups: rats under standard fixed lighting conditions (12 hours light/12 hours dark); rats under modelled dark deprivation with round-the-clock lighting (24 hours light); rats under modelled dark deprivation with round-the-clock lighting (24 hours light) receiving intragastric injections of linseed oil, melatonin or their combination from day 1 of the experiment. Serum concentrations of total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), total phospholipids (TPL) and atherogenic index (AI) were determined. Results. Long-term dark deprivation led to dyslipoproteinemia, which consists in an increase in serum concentrations of TC by a factor of 1.33 (p = 0.0009), TG by a factor of 1.62 (p = 0.013), LDL-C by a factor of 1.2 (p = 0.026) and TPL by a factor of 1.15 (p = 0.0082). The severity of changes in TC, TG, LDL-C, HDL-C and TPL concentrations varied depending on the duration of the experiment. During the use of linseed oil, melatonin or their combination under dark deprivation, the severity of disorders caused by desynchronosis decreased and lipid metabolism in rat serum normalized, especially at the initial stages of the research. Conclusion. Changes in lipid metabolism due to desynchronosis in rats injected with the substances under study were significantly smaller compared with animals that did not receive them. The most pronounced effects of administering these substances were observed in the group of rats treated with linseed oil and melatonin at the same time.

scholarly journals Oils’ Impact on Comprehensive Fatty Acid Analysis and Their Metabolites in Rats

Nutrients ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1232
Author(s):  
Agnieszka Stawarska ◽  
Małgorzata Jelińska ◽  
Julia Czaja ◽  
Ewelina Pacześniak ◽  
Barbara Bobrowska-Korczak
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Olive Oil ◽  
Edible Oils ◽  
Solid Phase ◽  
Linseed Oil ◽  
Standard Diet ◽  
Sprague Dawley ◽  
Rat Serum ◽  
Serum Fatty Acid

Fatty acids, especially polyunsaturated, and their metabolites (eicosanoids) play many pivotal roles in human body, influencing various physiological and pathological processes. The aim of the study was to evaluate the effect of supplementation with edible oils diverse in terms of fatty acid composition on fatty acid contents, activities of converting their enzymes, and on lipoxygenase metabolites of arachidonic and linoleic acids (eicosanoids) in rat serum. Female Sprague-Dawley rats divided into seven groups were used in the study. Animals from six groups were fed one of oils daily (carotino oil, made up by combining of red palm oil and canola oil, linseed oil, olive oil, rice oil, sesame oil, or sunflower oil). One group received a standard diet only. Fatty acids were determined using gas chromatography with flame ionization detection. Eicosanoids—hydroxyeicosatetraenoic (HETE) and hydroxyoctadecadienoic acids (HODE) were extracted using a solid-phase extraction method and analyzed with HPLC. Vegetable oils given daily to rats caused significant changes in serum fatty acid profile and eicosanoid concentrations. Significant differences were also found in desaturases’ activity, with the linseed and olive oil supplemented groups characterized by the highest D6D and D5D activity. These findings may play a significant role in various pathological states.

scholarly journals Effect of Diet Supplementation on the Expression of Bovine Genes Associated with Fatty Acid Synthesis and Metabolism

2010 ◽  
Vol 4 ◽  
pp. BBI.S4168 ◽  
Author(s):  
Sandeep J. Joseph ◽  
Kelly R. Robbins ◽  
Enrique Pavan ◽  
Scott L. Pratt ◽  
Susan K. Duckett ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Corn Oil ◽  
Fatty Acid Content ◽  
Fatty Acid Synthetase ◽  
Health Benefit ◽  
Acid Synthesis ◽  
Animal Origin ◽  
Expression Of Genes ◽  
Diet Supplementation

Conjugated linoleic acids (CLA) are of important nutritional and health benefit to human. Food products of animal origin are their major dietary source and their concentration increases with high concentrate diets fed to animals. To examine the effects of diet supplementation on the expression of genes related to lipid metabolism, 28 Angus steers were fed either pasture only, pasture with soybean hulls and corn oil, pasture with corn grain, or high concentrate diet. At slaughter, samples of subcutaneous adipose tissue were collected, from which RNA was extracted. Relative abundance of gene expression was measured using Affymetrix GeneChip Bovine Genome array. An ANOVA model nested within gene was used to analyze the background adjusted, normalized average difference of probe-level intensities. To control experiment wise error, a false discovery rate of 0.01 was imposed on all contrasts. Expression of several genes involved in the synthesis of enzymes related to fatty acid metabolism and lipogenesis such as stearoyl-CoA desaturase (SCD), fatty acid synthetase (FASN), lipoprotein lipase (LPL), fatty-acyl elongase (LCE) along with several trancription factors and co-activators involved in lipogenesis were found to be differentially expressed. Confirmatory RT-qPCR was done to validate the microarray results, which showed satisfactory correspondence between the two platforms. Results show that changes in diet by increasing dietary energy intake by supplementing high concentrate diet have effects on the transcription of genes encoding enzymes involved in fat metabolism which in turn has effects on fatty acid content in the carcass tissue as well as carcass quality. Corn supplementation either as oil or grain appeared to significantly alter the expression of genes directly associated with fatty acid synthesis.

scholarly journals Effects of the replacement of corn oil with linseed oil on fatty acid composition and the expression of lipogenic genes in broiler chickens

2014 ◽  
Vol 59 (No. 8) ◽  
pp. 353-364 ◽  
Author(s):  
W. Chen ◽  
R. Zhao ◽  
B.X. Yan ◽  
J.S. Zhang ◽  
Y.Q. Huang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Broiler Chickens ◽  
Corn Oil ◽  
Linseed Oil ◽  
Abdominal Fat ◽  
Breast Muscle ◽  
Feed Conversion ◽  
Dietary Pufa ◽  
Total N ◽  
Lipogenic Genes

The effect of dietary 18:2n-6/18:3n-3 ratio (by the replacement of corn oil with linseed oil) on n-3 polyunsaturated fatty acids (PUFA) enrichment in breast muscle of broiler chickens and the expression of lipogenic genes were investigated. Broiler chickens were fed ad libitum with diets containing 5% corn oil (CO), 3.75% corn oil + 1.25% linseed oil (CL1), 2.5% corn oil + 2.5% linseed oil (CL2), and 5% linseed oil (LO) based on the basic diets, respectively. Dietary 18:2n-6/18:3n-3 ratio did not affect 42-day body weight and 0–42-day feed conversion efficiency (feed/gain, P > 0.05) of broiler chickens, however, 5% linseed oil significantly increased 0–21-day feed conversion efficiency (feed/gain, P < 0.05) and decreased breast muscle weight (by 16%, P < 0.05) of broiler chickens. With the decrease of dietary 18:2n-6/18:3n-3 ratio, the enrichment of total n-3 PUFA, 18:3n-3, 20:5n-3, and 22:5n-3 increased linearly (P < 0.01), while the enrichment of total n-6 PUFA and 18:2n-6 decreased linearly (P < 0.01) in breast muscle of broiler chickens. Dietary corn oil increased the enrichment proportion of 20:4n-6 in a dosage-independent manner. Replacing 1.5% corn oil with linseed oil increased the enrichment proportion of 22:6n-3 (P < 0.05), but continuing to increase dietary linseed oil could not further elevate its deposition. Real-time quantitative RT-PCR was used to determine the expression of the mRNA levels of related genes. Dietary PUFA had insignificant effect on the expressions of LPIN2, WD and tetratricopeptide repeats 1 (WDTC1) and △-6 fatty acid desaturase (FADS2) in both breast muscle and abdominal fat. The effect of dietary PUFA on the expression of LPIN1 gene showed clear tissue dependence. Equivalent adding of corn oil and linseed oil could up-regulate the mRNA level of LPIN1 in abdominal fat (P < 0.01). This study demonstrated that decreasing dietary 18:2n-6/18:3n-3 ratio promoted the deposition of desirable n-3 long chain PUFA in the edible tissue and influenced the expression of LPIN1 in a tissue-dependent manner.  

THE INFLUENCE OF DIFFERENT DIETARY FATS ON THE REPRODUCTIVE PERFORMANCE OF TURKEY HENS

1990 ◽  
Vol 70 (2) ◽  
pp. 679-684 ◽  
Author(s):  
C. VILCHEZ ◽  
S. P. TOUCHBURN ◽  
E. R. CHAVEZ ◽  
C. W. CHAN
Keyword(s):  
Fatty Acid ◽  
Olive Oil ◽  
Dietary Fat ◽  
Egg Production ◽  
Corn Oil ◽  
Dietary Fats ◽  
Breeder Hens ◽  
Fat Composition ◽  
Feeding Diets ◽  
Fatty Acid Compositions

Turkey breeder hens of the Quebec line were fed diets with no added fat, 3.5% animal-vegetable fat blend (A-V fat), or 3.5% fat provided by 4.4% dry fat product (DF), from 30 to 49 wk of age. The addition of A-V fat and DF to the breeder diets significantly (P < 0.05) reduced embryonic mortality between 8 and 28 d of incubation but had no effect on other reproductive parameters. Feeding diets with 0 or 5% A-V fat in a prebreeder diet from 21 to 28 wk of age in another experiment led to a detectable decrease in subsequent egg production. Those diets were followed by breeder diets with either no added fat, 5% A-V fat, 5% corn oil, or 5% olive oil from 28 to 48 wk of age. Hens fed no added dietary fat exhibited significantly higher fertility while those fed 5% corn oil laid the heaviest eggs. Hens fed 5% olive oil exhibited significantly higher embryo livability from 8 to 28 d of incubation and the highest hatchability of fertile eggs. Fatty acid compositions of plasma and yolk lipids were affected by the dietary fat composition. Key words: Turkey hens, dietary fat, reproduction, fatty acid

scholarly journals Dietary ALA from Spinach Enhances Liver n-3 Fatty Acid Content to Greater Extent than Linseed Oil in Mice Fed Equivalent Amounts of ALA

Lipids ◽  
2015 ◽  
Vol 51 (1) ◽  
pp. 39-48 ◽  
Author(s):  
Miho Kuroe ◽  
Hiroyuki Kamogawa ◽  
Masashi Hosokawa ◽  
Kazuo Miyashita
Keyword(s):  
Fatty Acid ◽  
Acid Content ◽  
Linseed Oil ◽  
Fatty Acid Content

scholarly journals EFEK KONSUMSI BERBAGAI JENIS MINYAK GORENG TERHADAP KETEBALAN TUNIKA INTIMA PADA TIKUS JANTAN STRAIN WISTAR

2018 ◽  
Vol 5 (2) ◽  
pp. 163-169
Author(s):  
Tetes Wahyu Witradharma
Keyword(s):  
Fatty Acid ◽  
Olive Oil ◽  
Saturated Fatty Acid ◽  
Corn Oil ◽  
Blood Lipid ◽  
Male Rats ◽  
Protective Effects ◽  
Close Relationship ◽  
Tunica Intima ◽  
Chain Saturated Fatty Acid

CVD has close relationship with nutrition and food. But the results of another study did not support the close relationship between saturated fat intake with blood lipid levels and risk of CVD. The aim is to determine the effect of various types of fried oil consumption to the thickness of tunica intima in male rats of wistar strain. This study was an experimental research. The sample were 25 rats based on inclusion and exclusion criteria. Treatment groups were separated and each given long chain saturated fatty acid (LCSFA) cow fat, medium chain saturated fatty acid (MCSFA) VCO, polyunsaturated fatty acid (PUFA) corn oil and monounsaturated fatty acid (MUFA) olive oil orally 2.5 ml/day for 2 months. Data were obtained include the sample characteristics and thickness of tunica intima. The highest mean of tunica intima thickness was corn oil 16385,61±1715,79 nm and the lowest olive oil 12075,97±2464,34 nm (p=0,033). This study shows that the harmful effects of SFA and the protective effects of UFA to the thickness of tunica intima need to be corrected because not all SFA is bad and UFA is good for health. It is therefore recommended to inform about foods are beneficial for health.

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