Characteristic and antioxidant activity of Cladophora glomerata ethanolic extract as affected by prior chlorophyll removal and drying methods

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

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Antioxidant activity and nitrite scavenging ability of each fractions from Phyllostachys bambusoides ethanolic extract

Oriental Pharmacy and Experimental Medicine ◽

10.3742/opem.2005.5.3.209 ◽

2005 ◽

Vol 5 (3) ◽

pp. 209-215

Keyword(s):

Antioxidant Activity ◽

Ethanolic Extract ◽

Phyllostachys Bambusoides

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Effect of Drying Methods and Gamma Irradiation on the Color Changes and Antioxidant Activity of Grape By-Products

Journal of the Korean Society of Food Science and Nutrition ◽

10.3746/jkfn.2010.39.12.1826 ◽

2010 ◽

Vol 39 (12) ◽

pp. 1826-1831 ◽

Cited By ~ 6

Author(s):

Ji-Eun Jo ◽

Hong-Sun Yook ◽

Kyoung-Hee Kim ◽

Jong-Yeon Baek ◽

Young-Ja Moon ◽

...

Keyword(s):

Antioxidant Activity ◽

Gamma Irradiation ◽

Drying Methods ◽

Color Changes ◽

By Products

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Synergistic action of propolis with levodopa in the management of Parkinsonism in Drosophila melanogaster

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2019-0136 ◽

2020 ◽

Vol 17 (3) ◽

Author(s):

Emmanuel Tiyo Ayikobua ◽

Josephine Kasolo ◽

Keneth Iceland Kasozi ◽

Ejike Daniel Eze ◽

Abass Safiriyu ◽

...

Keyword(s):

Antioxidant Activity ◽

Drosophila Melanogaster ◽

Side Effects ◽

Motor Activity ◽

Ethanolic Extract ◽

Synergistic Action ◽

Content Type ◽

Treatment Groups ◽

Ethanolic Extract Of Propolis ◽

Different Doses

AbstractBackgroundThe Phosphatase and tensin-induced putative kinase 1 (PINK1B9) mutant for Drosophila melanogaster is a key tool that has been used in assessing the pathology of Parkinsonism and its possible remedy. This research was targeted toward determining the effects of ethanolic extract of propolis, with levodopa therapy in the management of Parkinsonism.MethodThe PINK1B9 flies were divided into groups and fed with the different treatment doses of ethanoic extract of propolis. The treatment groups were subjected to 21 days of administration of propolis and the levodopa at different doses after which percentage climbing index, antioxidant activity and lifespan studies were done.ResultsPropolis alone improved motor activity, antioxidant and lifespan in Drosophila melanogaster than in PINK1 flies. Propolis in combination with levodopa significantly (P<0.05) improved physiological parameters at higher than lower concentrations in Parkinsonism Drosophila melanogaster demonstrating its importance in managing side effects associated with levodopa.ConclusionPropolis is a novel candidate as an alternative and integrative medicinal option to use in the management of Parkinsonism in both animals and humans at higher concentrations.

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Effects of Cooking and Processing Methods on Phenolic Contents and Antioxidant and Anti-Proliferative Activities of Broccoli Florets

Antioxidants ◽

10.3390/antiox10050641 ◽

2021 ◽

Vol 10 (5) ◽

pp. 641

Author(s):

Hee Young Kim ◽

Meran Keshawa Ediriweera ◽

Kyung-Hwan Boo ◽

Chang Sook Kim ◽

Somi Kim Cho

Keyword(s):

Antioxidant Activity ◽

High Performance ◽

Radical Scavenging ◽

Steam Treatment ◽

Drying Methods ◽

Processing Methods ◽

Phenolic Contents ◽

Hot Air ◽

Freeze Dried ◽

Mcf 7

We investigated the effects of cooking (steaming and microwaving) and processing (freeze-drying and hot-air-drying) methods on the antioxidant activity of broccoli florets. 2,2-diphenyl-1-picrylhydrazyl (DPPH•), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•), and alkyl• free radical scavenging assays were employed to assess anti-oxidant potentials. The cytoprotective effect against oxidative damage induced by H2O2 was studied using hepatocellular carcinoma (HepG2) cells. Anti-proliferative effects were assessed in MCF-7 and MDA-MB-231 breast cancer cells. L-sulforaphane in broccoli extracts was quantified using high-performance liquid chromatography (HPLC). Steam and microwave treatments caused increases in total polyphenol content (TPC), whereas the total flavonoid content (TFC) decreased following steam treatment. A slight increase in TFC was observed in the microwaved samples. Extracts of all broccoli samples showed almost identical radical scavenging and cytoprotective effects. HPLC demonstrated that steamed (3 min)-freeze-dried (F-S3) and microwaved (2 min)-freeze-dried (F-M2) samples exhibited elevated levels of L-sulforaphane. In addition, the F-S3 and F-M2 extracts displayed strong anti-proliferative effects in MCF-7 cells, which correlated with L-sulforaphane content. As we observed no significant decrease in the antioxidant activity of broccoli florets, the cooking and processing methods and conditions studied here are recommended for broccoli.

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