Tyrosinase Inhibitory Activity of Flavonoids from Artocarpus Lowii King

2014 ◽  
Vol 71 (1) ◽  
Author(s):  
Shajarahtunnur Jamil ◽  
Siti Awanis Abdullah ◽  
Siti Mariam Abdul Lathiff ◽  
Hasnah Mohd Sirat
Keyword(s):  
Inhibitory Activity ◽  
Kojic Acid ◽  
Mushroom Tyrosinase ◽  
Tyrosinase Inhibitory Activity ◽  
Crude Extracts ◽  
Ic50 Value ◽  
Microplate Reader

Tyrosinase inhibitory activity was studied on the crude extracts and flavonoids successfully isolated from the leaves and heartwoods of Artocarpus lowii King. The flavonoids were fully characterized spectroscopically as isobavachalcone (1), 4-hydroxyonchocarpin (2), 2',4'-dihydroxy-4-methoxy-3'-prenyldihydrochalcone (3), 2',4'-dihydroxy-3,4-(2",2"-dimethylchromeno)-3'-prenyldihydrochalcone (4), artocarpin (5), cycloheterophyllin (6) and 4',5-dihydroxy-6,7-(2,2-dimethylpyrano)-2'-methoxy-8-γ,γ-dimethyl allylflavone (7). Tyrosinase inhibitory activity of the samples was determined against mushroom tyrosinase using ELISA microplate reader. Cycloheterophyllin (6) exhibited an excellent inhibitory activity against mushroom tyrosinase comparable to the standard kojic acid with the IC50 value of 52.5 µg/mL (88.3%).

scholarly journals STUDY OF TYROSINASE INHIBITORY ACTIVITY AND PHYTOCHEMICAL SCREENING OF CASSIA FISTULA L. LEAVES

2018 ◽  
Vol 10 (1) ◽  
pp. 384
Author(s):  
Caroline Wijaya ◽  
Berna Elya ◽  
Arry Yanuar
Keyword(s):  
Inhibitory Activity ◽  
Leaf Extract ◽  
Test Results ◽  
Phytochemical Screening ◽  
Cassia Fistula ◽  
Kinetic Assay ◽  
Tyrosinase Inhibitory Activity ◽  
Water Fraction ◽  
Ic50 Value ◽  
Phytochemical Constituents

Objective: This study was carried out to evaluate the phytochemical constituents and tyrosinase inhibitory activity of Cassia fistula leaves.Methods: A tyrosinase inhibitory activity assay was performed by measuring the decrease in the intensity of color suggestive of the inhibition ofdopachrome formation resulting from the L-DOPA-tyrosinase reaction.Results: The test results showed that the tyrosinase inhibitory activity of the water fraction of C. fistula leaf extract had the highest IC50 value(152.031 μg/mL) among other fractions (n-hexane, ethyl acetate, and n-butanol). An enzyme kinetic assay showed that the water fraction of C. fistulaleaf extract inhibited tyrosinase with mixed-type inhibition. Phytochemical screening showed that the water fraction of C. fistula leaf extract containedalkaloids, flavonoids, glycosides, phenols, and tannins.Conclusion: The current study indicated that C. fistula leaves possess significant tyrosinase inhibitory activity.

scholarly journals A New Phenolic Constituent From Carica papaya Flowers and Its Tyrosinase Inhibitory Activity

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1985098
Author(s):  
Giang Thi Kim Lien ◽  
Do Thi Thuy Van ◽  
Dao Hung Cuong ◽  
Pham Hai Yen ◽  
Bui Huu Tai ◽  
...  
Keyword(s):  
Spectral Data ◽  
Inhibitory Activity ◽  
Carica Papaya ◽  
Kojic Acid ◽  
Positive Control ◽  
Natural Source ◽  
Tyrosinase Inhibitory Activity ◽  
Esi Ms ◽  
Phenolic Constituent

A new phenolic (caricapapayol, 1) and 8 known compounds (2-9) were isolated from the flowers of Carica papaya. Their structures were determined by analysis of HR-ESI-MS, NMR spectral data, and comparison with the literature. Among known compounds, compound 2 has not been reported from natural source. Compounds 1, 2, and 4 exhibited tyrosinase inhibitory activity with IC50 values of 14.3 ± 2.7, 25.5 ± 1.9, and 19.8 ± 3.0 µM, respectively, in comparison with positive control kojic acid IC50 11.3 ± 1.6 µM.

Phytochemicals and Tyrosinase Inhibitory Activity from Piper caninum and Piper magnibaccum

2019 ◽  
Vol 25 (4) ◽  
pp. 358-363
Author(s):  
Nur Athirah Hashim ◽  
Farediah Ahmad ◽  
Wan Mohd Nuzul Hakimi Wan Salleh ◽  
Shamsul Khamis
Keyword(s):  
Inhibitory Activity ◽  
Kojic Acid ◽  
Biological Effects ◽  
Positive Control ◽  
Aromatic Plants ◽  
Tyrosinase Inhibitory Activity ◽  
Potential Source ◽  
Phytochemical Constituents ◽  
Reported Data ◽  
Ethyl Acetate Extracts

Background: Piper species are aromatic plants used as spices in the kitchen, but their secondary metabolites have also shown biological effects on human health. In traditional medicine, Piper species have been used worldwide to treat several diseases such as urological problems, skin, liver and stomach ailments, for wound healing, and as antipyretic and anti-inflammatory agents. In the present study, we attempted to isolate the phytochemicals from Piper caninum and Piper magnibaccum and evaluate their tyrosinase inhibitory activity. Methods: Phytochemical constituents of the extracts were investigated using various chromatographic and spectroscopic methods. The structures of the isolated phytochemicals were established by analysis of their spectroscopic data, as compared to that of reported data. Tyrosinase inhibitory activity was also tested on the extracts and selected compounds using mushroom tyrosinase as the enzyme. Results: Fractionation and purification of the extracts of Piper caninum and Piper magni­baccum afforded nine known compounds which were cepharanone A (1), cepharadione A (2), aristolactam AII (3), 5,7-dimethoxyflavone (4), 24-methylenecycloartan-3-one (5), β-sitosterol (6), piperumbellactam A (7), 24S-ethylcholesta-5,22,25-trien-3β-ol (8) and stigmast-3,6-dione (9). Ethyl acetate extracts from leaves of P. magnibaccum gave the highest inhibition value at 48.35%, while the tested compounds displayed weak tyrosinase activity compared to the positive control, kojic acid. Conclusion: These phytochemical results suggested that the extracts could assist as a potential source of bioactive compounds. Further research is needed in which the extract could possibly be exploited for pharmaceutical use.

Tyrosinase Inhibitory Activity of Pyrazole Derivatives

2012 ◽  
Vol 506 ◽  
pp. 194-197
Author(s):  
T. Suwunwong ◽  
T. Kobkeatthawin ◽  
K. Chanawanno ◽  
N. Saewan ◽  
P. Wisitsak ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Spectroscopic Data ◽  
Kojic Acid ◽  
Data Interpretation ◽  
Pyrazole Derivatives ◽  
Tyrosinase Inhibitory Activity

A series of 3,5-substituted-4,5-dihydro-1H-pyrazole-1-carbothioamide derivatives were synthesized. Their structures were determined on the basis of spectroscopic data interpretation and their tyrosinase inhibitory activity was determined. The results showed that compound 2 (at 1.00 mg/mL) exhibits significant tyrosinase inhibitory activity with % inhibition of 91.866 ± 2.086 with L-tyrosine as substrate whereas compound 3 (at 1.00 mg/mL) exhibits significant tyrosinase inhibitory activity with % inhibition of 79.266 ± 0.552 and 89.593 ± 1.015 with L-tyrosine and L-DOPA as substrates. The IC50values of compounds 2 and 3 were further determined comparing with kojic acid and resveratrol. It was found that IC50values of compounds 2 and 3 were 0.391 ± 0.017 and 0.259 ± 0.005 mg/mL., with L-tyrosine as substrate, which were lower than those of the standard tyrosinase inhibitory resveratrol (0.965 ± 0.016 mg/mL).

scholarly journals AKTIVITAS INHIBITOR TIROSINASE PADA EKSTRAK ALGA COKELAT SARGASSUM SP. AGARDH ASAL PESISIR LHOK BUBON, KABUPATEN ACEH BARAT

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Muhammad Gazali
Keyword(s):  
Inhibitory Activity ◽  
Brown Algae ◽  
Methanol Extract ◽  
Kojic Acid ◽  
Bioactive Compound ◽  
Positive Control ◽  
Marine Macroalgae ◽  
Tyrosinase Inhibitor ◽  
Tyrosinase Inhibitory Activity ◽  
New Finding

Seaweeds are marine macro algae that can be found attach to the bottom shallow coastal waters with subsrate as attached media. There are three major groups of seaweeds namely brown algae (Phaeophyta), red (Rhodophyta) and green (Chlorophyta). Sargassum sp is one of brown algae which mostly found in the Lhok Bubon Coastal West of Aceh. Recently, exploration of marine macroalgae as bioactive sources was investigated. Seaweed contains bioactive compound which can serve as a defense from ultraviolet radiation that caused hyperpigmentation effect. The aiming of this study is to analyse the tyrosinase inhibitory activity of Sargassum sp extract from Lhok Bubon Coastal Area, West of Aceh. The results shown that the methanol extract of Sargassum sp possess phytochemical properties such as fenol, alkaloid and triterpenoid. Tyrosinase inhibitory activity of Sargassum sp methanol extract  is the best extract which can be inhibit  monophenolase with  IC50 : 1111.49 µg/ml and IC50 = 1582.31 µg/ml in diphenolase pathway with kojic acid as positive control. Moreover, etyl asetate and n-hexane extract have no activity of tyrosinase inhibitor. Therefore, new finding of tyrosinase inhibitor agent from marine macroalgae Sargassum sp give the fruitfull information for cosmeceutical industry.Keywords : Lhok Bubon, Brown Algae, Sargassum sp, Tyrosinase Inhibitor

scholarly journals Phenolic Constituents from the Heartwood of Artocapus Altilis and their Tyrosinase Inhibitory Activity

2012 ◽  
Vol 7 (2) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Mai Ha Khoa Nguyen ◽  
Hai Xuan Nguyen ◽  
Mai Thanh Thi Nguyen ◽  
Nhan Trung Nguyen
Keyword(s):  
Phenolic Compounds ◽  
Vanillic Acid ◽  
Inhibitory Activity ◽  
Kojic Acid ◽  
Positive Control ◽  
Coumaric Acid ◽  
Meoh Extract ◽  
Tyrosinase Inhibitory Activity ◽  
Phenolic Constituents ◽  
Inhibitory Activities

From the MeOH extract of the heartwood of Artocapus altilis, thirteen phenolic compounds have been isolated, namely curcumin (1), desmethoxycurcumin (2), retrodihydrochalcone (3), apigenin (4), tangeretin (5), nobiletin (6), O-methyldehydrodieugenol (7), dehydrodieugenol (8), β-hydroxypropiovanillone (9), p-coumaric acid (10), p-hydroxybenzaldehyde (11), vanillin (12), and vanillic acid (13). This is the first report on the presence of these compounds in the heartwood of A. altilis. Compounds 1, 2, and 10 showed more potent tyrosinase inhibitory activities, with IC50 values ranging from 2.3 to 42.0 μM, than the positive control kojic acid (IC50, 44.6 μM). The most active compound, p-coumaric acid (10) (IC50, 2.3 μM), was 22 times more active in tyrosinase inhibitory activity than kojic acid.

scholarly journals AKTIVITAS INHIBISI ENZIM ALFA-GLUKOSIDASE DARI EKSTRAK KULIT BATANG BUNGA KUPU-KUPU (Bauhinia semibifida Roxb) SECARA IN VITRO

2021 ◽  
Vol 6 (2) ◽  
pp. 223-231
Author(s):  
Haiyul Fadhli ◽  
◽  
Nofri Hendri Sandi ◽  
Ainun Nurain Nurdin
Keyword(s):  
Ethyl Acetate ◽  
Inhibitory Activity ◽  
Stem Bark ◽  
Positive Control ◽  
Bark Extract ◽  
In Vitro Testing ◽  
Ic50 Value ◽  
Microplate Reader ◽  
Stem Bark Extract

Research on the activity of ?-glucosidase inhibition of Bunga Kupu-Kupu stem bark extract (Bauhinia semibifida Roxb) has been carried out in vitro. This study aims to determine the inhibitory activity of the extracts of B. semibifida Roxb. stem bark against the ?-glucosidase enzyme in vitro. Testing the inhibitory activity of the ?-glucosidase enzyme using extracts of n-hexane, ethyl acetate, and methanol from the stem bark of the Bunga Kupu-Kupu and the Akarbose as a positive control. In vitro testing was carried out using a microplate reader instrument with a wavelength of 410 nm. The results showed that the extract of n-hexane extract of the B. semibifida stem bark had an IC50 value 15,625 µg/mL, ethyl acetate extract had an IC50 value 35,495 µg/mL, and the methanol extract had an IC50 value 34,279 µg/mL. By category, the three B. semibifida stem bark extracts have the active ability to inhibit the ?-glucosidase enzyme, while the Akarbose had an IC50 value 0,384 µg/mL as a positive control has a very active ability as an antidiabetic through the inhibition of the ?-glucosidase enzyme. The results showed that extraction of ?-glucosidase inhibitor compound with n-hexane yielded extract with highest inhibitor activity.

scholarly journals Preliminary Screening of Selected Plant Extracts for Anti Tyrosinase Activity

2016 ◽  
Vol 16 (1) ◽  
pp. 18 ◽  
Author(s):  
Vidyalakshmi Subramanian ◽  
Dhamodharan Sahithya
Keyword(s):  
Inhibitory Activity ◽  
Kojic Acid ◽  
Control Cell ◽  
Punica Granatum ◽  
Positive Control ◽  
Tyrosinase Inhibition ◽  
Tyrosinase Inhibitory Activity ◽  
Cassia Angustifolia ◽  
Important Approach

Tyrosinase inhibition is an important approach towards controlling hyper pigmentation. We aimed to screen alcoholic extracts of 11 plants extract for their tyrosinase inhibitory activity. These plants have been used traditionally in the treatment of skin ailments and for the improvement of skin complexion. The extracts were quantified for total phenols, alkaloids and tannins. <em>In vitro</em> tyrosinase inhibition was performed with kojic acid as the positive control. Cell viability was tested on B16 F0 melanoma cells. The extracts of <em>Rosa berberifolia</em>, <em>Punica granatum</em> and <em>Casiia angustifolia</em> showed more than 80% inhibition at 500 mg/ml concentration. Nine of the extracts were also shown to have a high phenolic content greater than 200 mg/g of the plant material. The tyrosinase inhibitory activity of the extracts of <em>Cassia angustifolia, Punica granatum </em>and<em> Rosa berberifolia</em> were comparable with that of the control, kojic acid. The three extracts also showed lesser than 50% cytotoxicity at the concentrations tested. From the screening assays, it is seen that three plants have appreciable tyrosinase inhibitory activity. Hence, these plants may be further evaluated for their use in cosmetics and hyper pigmentation.

Sequential injection spectrophotometric system for evaluation of mushroom tyrosinase-inhibitory activity

Talanta ◽  
2012 ◽  
Vol 101 ◽  
pp. 233-239 ◽  
Author(s):  
Nuntaporn Moonrungsee ◽  
Tomoko Shimamura ◽  
Takehiro Kashiwagi ◽  
Jaroon Jakmunee ◽  
Keiro Higuchi ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Mushroom Tyrosinase ◽  
Sequential Injection ◽  
Tyrosinase Inhibitory Activity
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