scholarly journals Water-soluble chitosan preparation from marine sources

2019 ◽  
Vol 15 (2) ◽  
pp. 159-163 ◽  
Author(s):  
Yatim Lailun Ni'mah ◽  
Harmami Harmami ◽  
Ita Ulfin ◽  
Suprapto Suprapto ◽  
Clarissa Welny Saleh

Water-soluble chitosan (WSC) has been derived from different marine sources such as squid pen, shrimp, mussel, and crab shells. Chitosan was obtained by deacetylation of chitin. Degradation of chitosan by hydrogen peroxide would produce WSC. Demineralization process was optimized by varying incubation time and temperature to minimize the mineral content in chitin. WSC that obtained was characterized by Fourier-transform infrared spectroscopy (FTIR) to calculate the deacetylation degree. Deacetylation degree of WSC was also studied by titrimetric method. The ash content of chitin from marine sources was measured to obtain the optimum demineralization incubation time. The optimum incubation time for demineralization process was 12 hours and stirred at 50 rpm. Recovery and deacetylation degree of WSC were found to be varied and depended on the source of WSC. Each sample of WSC from shrimp shell, mussel shell, squid pen and crab shell has the degree of deacetylation of 64.18%, 35.03%, 58.04% and 53.91% respectively. The presence of amine group was confirmed from FTIR spectra ofsynthesized WSC.

1967 ◽  
Vol 39 (3) ◽  
pp. 166-182
Author(s):  
Aarne Mäkelä ◽  
Kaija Kotilainen

This paper deals with ash and mineral contents essential in the nutrition of livestock and man. The results of the investigated products are presented in Tables 1—3. Of the minerals the ash and the water-soluble part of it were determined, as well as the ash of the water-soluble fraction and the cations and anions P, Cl, K, Na, Ca and Mg. The determination of the water-solubility to some extent characterizes in what form the minerals occur in the plant. The following results were obtained in the determinations of the contents of total ash and the water-solubility. 1. As the Gramineae-grasses grow older the contents of ash and of individual minerals decrease, while the water-solubility simultaneously slightly declines (Table 1). The water-solubility of the ash and P is 70—80 %, that of K almost complete, that of Ca c. 50 % and that of Mg in general 60—70 %. 2. When red clover develops from the leaf stage to the blooming stage the contents of ash, N, P, Na, Ca and Mg decrease considerably, even to one half, while the content of K remains unchanged (Table 2). Simultaneously one can observe a decrease in the water-solubility. The water-solubility of the ash is 50—55 %, that of P 70—80 %, that of K almost complete, that of Ca 30—50 % and that of Mg 50—70 %. The contents of N, P and Ca in the leaves are considerably higher than in the stalks; at the blooming stage this is true also for Mg. On the other hand the content of K in the stalks is higher than in the leaves. The mineral content of the flowers is similar to that of the leaves, except that the content of ash is lower and that of phosphorus higher. 3. The water-solubility of the ash of cereal grains, cereal products and straws is 40—60 % (Table 2). The water-solubility of P in grains and wheat bran is c. 40 %, in brewers’ grains and straw 60—80 %. The water-solubility of K is only 60—80 %, except in straw where it is 90 %. The water-solubility of Ca in grains and brewers’ grains is c. 70 %, in the wheat bran and straw 30—40 %, in hulls of oats 20 % and awns of barley 66 %. The water-solubility of Mg in brewers’ grains is almost 70%, in oat grains and straw c. 50 %, in wheat grains and bran 20—30 %. 4. The water-solubility of the ash of roots and potato (Table 3) is high, 80—90 %. The ash content of tops and potato stalks is quite high, but the water-solubility of the ash only approximately 60 %. The water-solubility of P of the aforementioned plants and parts of plants is 70—90 % and that of K almost complete. The water-solubility of Ca in potato tubers, swede tops and spring rape is c. 50 %, where as it is low in other plants, especially in sugar beet tops and potato stalks. The water-solubility of Mg in potato tubers and tops is c. 70 %, whereas it is low in carrots, potato stalks and especially in swede roots. 5. In Table 3 are furthermore presented the mineral contents of some plants belonging to various groups of plants which to a limited extent may be used by some animals. The dandelion (Taraxacum officinale) is quite similar to the meadow fescue at the leaf stage with respect to its mineral content and the water-solubility of various minerals. The common sedge (Carex Goodenowii) is with respect to its mineral content and the water-solubility of the minerals similar to the blooming timothy, except; that the amount and water-solubility of P is low. The water-soluble ash and the contents and water-solubility of various minerals of water horsetail (Equisetum fluviatile) and common horsetail (E. arvense) are approximately the same as those of the meadow fescue at the leaf stage, except that the contents of Ca and Mg are higher and the water-solubility of Ca considerably less. The ash content of chickweed (Stellaria media) (c. 24 % in the dry matter) and of its specific minerals, the content of K (c. 10 %) is exceptionally high. On the other hand lichen (Cladonia alpestris) is especially poor in minerals.


2011 ◽  
Vol 8 (2) ◽  
pp. 33
Author(s):  
Norfaezah Mazalan ◽  
Mazatulikhma Mat Zain ◽  
Nor Saliyana Jumali ◽  
Norhanim Mohalid ◽  
Zurina Shaameri ◽  
...  

Recently, research and development in the field of drug delivery systems (DDS) facilitating site-specific therapy has reached significant progression. DDS based on polymer micelles, coated micro- and nanoparticles, and various prodrug systems including water-soluble polymer have been prepared and extensively studied as novel drugs designed for cancer chemotherapy and brain delivery. Since polymers are going to be used in human, this study has the interest of testing two types of polymer, polyimides (PI) and polyphenylenevinylene (PPV) on neuronal cells. The objective of this study was to determine the possible neurotoxicity and potential neuroprotective effects of PI and PPV towards SH-SY5Y neuronal cells challenged by hydrogen peroxide (H2O2) as an oxidant. Cells were pretreated with either PI or PPV for 1 hour followed by incubation for 24 hour with 100 µM of H2O2. MTS assay was used to assess cell viability. Results show that PI and PPV are not harmful within the concentration up to 10 µM and 100 µM, respectively. However, PI and PPV do not protect neuronal cells against toxicity induced by H2O2 or further up the cell death.


2019 ◽  
Vol 10 (3) ◽  
pp. 2132-2138
Author(s):  
Virsa Handayani ◽  
Rezki Amriati Syarif ◽  
Ahmad Najib ◽  
Aktsar Roskiana Ahmad ◽  
Abdullah Mahmud ◽  
...  

Mahogany (Swietenia mahagoni (L.) Jacq) is one of the plants that is often used by the community as traditional medicine. One of them is antifungal, antibacterial, antidiabetic, and eczema. This study aims to obtain standardized extracts from mahogany seeds and leaves. Standardization of purified extract of mahogany has been carried out according to the monographs of extract standardization guidelines, which include testing of specific and non-specific parameters. The results of the specific parameter testing showed that the purified extract of mahogany seeds is a thick extract, brown to reddish, smells distinctive and has a bitter taste. While the purified extract of mahogany leaves is a thick extract, greenish-brown in color, distinctive smell and has a bitter taste. The chemical content of purified extract of mahogany seeds and leaves showed the presence of flavonoids, alkaloids, terpenoids and saponins. Water-soluble essence levels in mahogany seeds and leaves was 14.84% and 10.28%. While the ethanol-soluble essence levels in mahogany seeds and leaves were 15.38% and 12.43%. Testing of non-specific parameters on mahogany seeds and leaves showed the results of drying shrinkage levels of 0.22% and 8.84%, moisture content of 2.60% and 4.04%, total ash content of 1.71% and 1.93%, levels acidic insoluble ash 0.38% and 0.32%, Total Plate Number (ALT) of mahogany seed bacteria 1x102 colonies/g, Number of mahogany mold seeds 4x10 colonies/g, heavy metal lead contamination and cadmium in mahogany seeds 0.0607µg/g and<0.003µg/g. The inhibitory diameter of each concentration of seeds against Escherichia coli, 3%, 5%, 7%, and 9%, is 12,67; 13,67; 17,67; and 19,67 mm, respectively. The inhibitory diameter of each concentration of leaves against Escherichia coli, 3%, 5%, 7%, and 9%, is 10,27; 10,90; 13,46; and 15,68 mm, respectively.


1999 ◽  
Vol 69 (12) ◽  
pp. 956-960 ◽  
Author(s):  
J. Tokuda ◽  
R. Ohura ◽  
T. Iwasaki ◽  
Y. Takeuchi ◽  
A. Kashiwada ◽  
...  

2009 ◽  
Vol 2009 ◽  
pp. 1-5 ◽  
Author(s):  
Jeanette M. Cardamone ◽  
Alberto Nuñez ◽  
Rafael A. Garcia ◽  
Mila Aldema-Ramos

Keratin from wool is a reactive, biocompatible, and biodegradable material. As the biological structural component of skin (soft keratins) and of nails, claws, hair, horn, feathers, and scales (hard keratins) pure keratin comprises up to 90% by weight of wool. Wool was treated in alkaline solutions to extract from 68% to 82% keratin within 2 to 5 hours of exposure at . The keratin products were water-soluble and were confirmed to contain intermediate filament and microfibrillar component-proteins of fractured, residual cuticle, and cortical cells. Oxidation of wool by peroxycarboximidic acid in alkaline hydrogen peroxide produced keratin products with distinct microcrystalline structures: descaled fibers, fibrous matrices, and lyophilized powders. Morphology and confirmation of peptide functionality were documented by SEM, Amino Acid Analysis, SDS-PAGE gel electrophoresis, MALDI-TOF/TOF, and FTIR analyses. The reactivity of keratin from wool models the reactivity of keratin from low-value sources such as cattle hair.


2021 ◽  
Vol 1 (1) ◽  
pp. 1-8
Author(s):  
Andini Andini ◽  
Cindy Fernanda Putri

Mango peel (Mangfera indica L.) has many pharmacological effects as a traditional medicine. Therefore, standardization of mango peel simplisia needs to be done as a preparation of phytopharmaca raw material. This research aimed to obtain standardization of mango peel simplisia include specific and non-specific parameter. The research procedures include plant determination, simplisia preparation as well as specific standardization test (includes organoleptic, water-soluble compound concentration, and ethanol solution compound concentration) and nonspecific standardization test (includes moisture content, dried shrinkage, total ash content and acid insoluble ash content). The specific organoleptic parameters of dried mango peel simplisia have a distinctive sweet aroma, bitter taste, and brownish yellow colour. Water-soluble and ethanol-soluble concentrations are 22,36% ± 1,17% and 9,56% ± 0,07%. Moisture content is 9,09% ± 1,44%. Dried shrinkage rate is 0,19% ± 0,04%. Total ash and acid insoluble ash contents are 4,11% ± 0,10% and 0,14% ± 0,03%. The mango peel simplisia has met the quality standard of the raw material.


2017 ◽  
Vol 5 (1) ◽  
pp. 61
Author(s):  
Weon-Bae Ko ◽  
Hong-Seok Jeong ◽  
Sung-Ho Hwang

<p>The water-soluble fullerenes [C<sub>60</sub>, C<sub>70</sub>] are prepared with fullerenes [C<sub>60</sub>, C<sub>70</sub>] and a mixture of oxidants (v/v) at the ratio of 3:1 under ultrasonic condition at room temperature. The MALDI-TOF MS confirmed that the water-soluble compounds were C<sub>60</sub> and C<sub>70</sub>. The antioxidant effect of water-soluble fullerenes [C<sub>60</sub>, C<sub>70</sub>] in the PC 12 cells (Rat pheochromocytoma) line following exposure to hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) was investigated.</p>


Catalysts ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1078
Author(s):  
Hang Zhang ◽  
Zhipeng Ma ◽  
Yunpeng Min ◽  
Huiru Wang ◽  
Ru Zhang ◽  
...  

Several kinds of composite materials with phosphotungstic acid (PTA) as the catalyst were prepared with activated carbon as support, and their structures were characterized. According to the Box–Behnken central combination principle, the mathematical model of the heterogeneous system is established. Based on the single-factor experiments, the reaction temperature, the reaction time, the amount of hydrogen peroxide and the loading capacity of PTA were selected as the influencing factors to study the catalyzed oxidation of hydrogen peroxide and degradation of high molecular weight chitosan. The results of IR showed that the catalyst had a Keggin structure. The results of the mercury intrusion test showed that the pore structure of the supported PTA catalyst did not change significantly, and with the increase of PTA loading, the porosity and pore volume decreased regularly, which indicated that PTA molecules had been absorbed and filled into the pore of activated carbon. The results of Response Surface Design (RSD) showed that the optimum reaction conditions of supported PTA catalysts for oxidative degradation of high molecular weight chitosan by hydrogen peroxide were as follows: reaction temperature was 70 ℃, reaction time was 3.0 h, the ratio of hydrogen peroxide to chitosan was 2.4 and the catalyst loading was 30%. Under these conditions, the yield and molecular weight of water-soluble chitosan were 62.8% and 1290 Da, respectively. The supported PTA catalyst maintained high catalytic activity after three reuses, which indicated that the supported PTA catalyst had excellent catalytic activity and stable performance compared with the PTA catalyst.


Sign in / Sign up

Export Citation Format

Share Document