Wide-angle, high-resolution three-dimensional (3D) imaging using non-mechanical beam steering

2021 ◽  
Author(s):  
Christopher L. Hoy ◽  
Jay Stockley ◽  
Kelly Kluttz ◽  
Doug McKnight ◽  
Lance Hosting ◽  
...  
Keyword(s):  
High Resolution ◽  
3D Imaging ◽  
Beam Steering ◽  
Three Dimensional ◽  
Wide Angle

High Resolution Solid State Acoustic Imaging for Advanced Well Integrity and Deformation Assessments in Conventional and Unconventional Wells

2021 ◽  
Author(s):  
Thomas Littleford ◽  
Anthony Battistel ◽  
Greer Simpson ◽  
Kacper Wardynski
Keyword(s):  
Solid State ◽  
High Resolution ◽  
Wall Thickness ◽  
Beam Steering ◽  
Three Dimensional ◽  
Field Studies ◽  
Acoustic Imaging ◽  
Single Element ◽  
Imaging Technology ◽  
Well Integrity

Abstract An advanced high-resolution acoustic imaging technology was deployed for well integrity and deformation assessments in both vertical and horizontal wells. This high frequency acoustic tool collected three-dimensional data quantifying deformation and wall thickness with resolution unobtainable by existing multi-finger caliper, magnetic flux leakage, and rotating single element ultrasonic systems. Several novel imaging methods are enabled by the high number of transducers (up to 512) on the imaging probe. These methods, including beam forming, beam steering and semi-stochastic multipulse imaging, are outlined and discussed in this paper. In addition, multiple types of standardized visualizations enabled by this high-resolution 3D data capture tool are introduced and examples of each are shown. Lab qualification and imagery generated by the high-resolution solid-state imaging technology, when applied to various precision machined geometric anomalies, are presented. In addition to lab validation results, several field studies are showcased including assessments of ovalized casing, complex downhole corrosion, and isolated minor pitting. Leak paths, splits, and damaged regions within threaded casing collars were also identified, imaged, and quantified using the acoustic technology. Until now, these collar regions have been very difficult to image using legacy downhole tools due to fundamental limitations at the threaded connection geometry. Lastly, various downhole completion equipment case studies are presented showcasing several applications of acoustic imaging used to validate the set-position or condition of specialty downhole equipment. This paper outlines the usage of the solid-state acoustic technology to generate three dimensional geometry and wall thickness datasets with sub-millimetric resolution, providing operators with a holistic and actionable assessment of their well integrity.

Three Dimensional Imaging of Microelectronic Devices Using a CrossBeam FIB

2004 ◽  
Author(s):  
Eric Lifshin ◽  
James Evertsen ◽  
Edward Principe ◽  
John Friel
Keyword(s):  
High Resolution ◽  
3D Imaging ◽  
Ion Beam ◽  
Three Dimensional ◽  
Serial Sections ◽  
Microelectronic Devices ◽  
3D Microscopy ◽  
Internal Structures ◽  
Insight Into

Abstract Increased insight into the internal structure of microelectronic devices can be achieved through the use of three dimensional (3D) imaging based on image stacks of serial sections obtained with a combined electron and ion beam (CrossBeam) FIB. This study describes how such data can be collected and presented, some of the factors that need to be optimized to get the best images, and the limitations of the method. It can be viewed as a first step in the emerging area of high resolution 3D microscopy, a technique that can lead to more accurate characterization of the shapes of internal structures and their interconnectivity at the nanoscale.

scholarly journals Electron cryo-tomography of vestibular hair-cell stereocilia

2018 ◽  
Author(s):  
Zoltan Metlagel ◽  
Jocelyn F Krey ◽  
Junha Song ◽  
Mark F Swift ◽  
William J Tivol ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Hair Cell ◽  
3D Imaging ◽  
Three Dimensional ◽  
Vestibular Function ◽  
Structural Features ◽  
High Resolution Imaging ◽  
Native State ◽  
Resolution Imaging

High-resolution imaging of hair-cell stereocilia of the inner ear has contributed substantially to our understanding of auditory and vestibular function. To provide three-dimensional views of the structure of stereocilia cytoskeleton and membranes, we developed a method for rapidly freezing unfixed stereocilia on electron microscopy grids, which allowed subsequent 3D imaging by electron cryo-tomography. Structures of stereocilia tips, shafts, and tapers were revealed, demonstrating that the actin paracrystal was not perfectly ordered. This sample-preparation and imaging procedure will allow for examination of structural features of stereocilia in a near-native state.

scholarly journals Multi-fluorescence high-resolution episcopic microscopy (MF-HREM) for three dimensional imaging of adult murine organs

2020 ◽  
Author(s):  
Claire Walsh ◽  
Natalie Holroyd ◽  
Eoin Finnerty ◽  
Sean G. Ryan ◽  
Paul W. Sweeney ◽  
...  
Keyword(s):  
High Resolution ◽  
3D Imaging ◽  
Three Dimensional ◽  
Image Resolution ◽  
Biological Research ◽  
Wide Field ◽  
Vascular Networks ◽  
Optical Sectioning ◽  
Trade Offs ◽  
Xenograft Models

AbstractThree-dimensional microscopy of large biological samples (>0.5 cm3) is transforming biological research. Many existing techniques require trade-offs between image resolution and sample size, require clearing or use optical sectioning. These factors complicate the implementation of large volume 3D imaging. Here we present Multi-fluorescent High Resolution Episcopic Microscopy (MF-HREM) which allows 3D imaging of large samples without the need for clearing or optical sectioning.MF-HREM uses serial-sectioning and block-facing wide-field fluorescence, without the need for tissue clearing or optical sectioning. We detail developments in sample processing including stain penetration, resin embedding and imaging. In addition, we describe image post-processing methods needed to segment and further quantify these data. Finally, we demonstrate the wide applicability of MF-HREM by: 1) quantifying adult mouse glomeruli. 2) identifying injected cells and vascular networks in tumour xenograft models; 3) quantifying vascular networks and white matter track orientation in mouse brain.

High Resolution Microscopy of Multi-Layered Biological Crystals

1982 ◽  
Vol 40 ◽  
pp. 80-83
Author(s):  
H.A. Cohen ◽  
T.W. Jeng ◽  
W. Chiu
Keyword(s):  
High Resolution ◽  
Low Dose ◽  
Three Dimensional ◽  
Data Interpretation ◽  
Two Dimensional ◽  
Biological Objects ◽  
Density Maps ◽  
Density Map ◽  
Complex Crystal ◽  
High Resolution Microscopy

This tutorial will discuss the methodology of low dose electron diffraction and imaging of crystalline biological objects, the problems of data interpretation for two-dimensional projected density maps of glucose embedded protein crystals, the factors to be considered in combining tilt data from three-dimensional crystals, and finally, the prospects of achieving a high resolution three-dimensional density map of a biological crystal. This methodology will be illustrated using two proteins under investigation in our laboratory, the T4 DNA helix destabilizing protein gp32*I and the crotoxin complex crystal.

Electron crystallographic determination of the 3-D structure of staurolite at atomic resolution

1991 ◽  
Vol 49 ◽  
pp. 540-541
Author(s):  
Kenneth H. Downing ◽  
Hu Meisheng ◽  
Hans-Rudolf Went ◽  
Michael A. O'Keefe
Keyword(s):  
High Resolution ◽  
Three Dimensional ◽  
High Resolution Electron Microscopy ◽  
Atomic Resolution ◽  
Dimensional Structure ◽  
Structure Information ◽  
Resolution Electron ◽  
Scattering Effects ◽  
High Resolution Images ◽  
Effects Of Radiation

With current advances in electron microscope design, high resolution electron microscopy has become routine, and point resolutions of better than 2Å have been obtained in images of many inorganic crystals. Although this resolution is sufficient to resolve interatomic spacings, interpretation generally requires comparison of experimental images with calculations. Since the images are two-dimensional representations of projections of the full three-dimensional structure, information is invariably lost in the overlapping images of atoms at various heights. The technique of electron crystallography, in which information from several views of a crystal is combined, has been developed to obtain three-dimensional information on proteins. The resolution in images of proteins is severely limited by effects of radiation damage. In principle, atomic-resolution, 3D reconstructions should be obtainable from specimens that are resistant to damage. The most serious problem would appear to be in obtaining high-resolution images from areas that are thin enough that dynamical scattering effects can be ignored.

Design and calibration of an IVEM for general 3-dimensional imaging of non-diffracting materials

1992 ◽  
Vol 50 (2) ◽  
pp. 1040-1041
Author(s):  
Neil Rowlands ◽  
Jeff Price ◽  
Michael Kersker ◽  
Seichi Suzuki ◽  
Steve Young ◽  
...  
Keyword(s):  
3D Imaging ◽  
Tomographic Reconstruction ◽  
Three Dimensional ◽  
3 Dimensional ◽  
3D Microstructure ◽  
Image Translation ◽  
Digital Correlation ◽  
On Line ◽  
Mechanical Stage ◽  
Projection Angle

Three-dimensional (3D) microstructure visualization on the electron microscope requires that the sample be tilted to different positions to collect a series of projections. This tilting should be performed rapidly for on-line stereo viewing and precisely for off-line tomographic reconstruction. Usually a projection series is collected using mechanical stage tilt alone. The stereo pairs must be viewed off-line and the 60 to 120 tomographic projections must be aligned with fiduciary markers or digital correlation methods. The delay in viewing stereo pairs and the alignment problems in tomographic reconstruction could be eliminated or improved by tilting the beam if such tilt could be accomplished without image translation.A microscope capable of beam tilt with simultaneous image shift to eliminate tilt-induced translation has been investigated for 3D imaging of thick (1 μm) biologic specimens. By tilting the beam above and through the specimen and bringing it back below the specimen, a brightfield image with a projection angle corresponding to the beam tilt angle can be recorded (Fig. 1a).

Three-Dimensional Immunoelectron Microscopy of Yeast Cells by a New High-Resolution Replica Method

1985 ◽  
Vol 43 ◽  
pp. 562-563
Author(s):  
Hirano T. ◽  
M. Yamaguchi ◽  
M. Hayashi ◽  
Y. Sekiguchi ◽  
A. Tanaka
Keyword(s):  
High Resolution ◽  
Plasma Polymerization ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Replica Method ◽  
Yeast Cells ◽  
Dynamic Aspect ◽  
Replica Technique ◽  
Gaseous Hydrocarbon ◽  
Transmission Electron

A plasma polymerization film replica method is a new high resolution replica technique devised by Tanaka et al. in 1978. It has been developed for investigation of the three dimensional ultrastructure in biological or nonbiological specimens with the transmission electron microscope. This method is based on direct observation of the single-stage replica film, which was obtained by directly coating on the specimen surface. A plasma polymerization film was deposited by gaseous hydrocarbon monomer in a glow discharge.The present study further developed the freeze fracture method by means of a plasma polymerization film produces a three dimensional replica of chemically untreated cells and provides a clear evidence of fine structure of the yeast plasma membrane, especially the dynamic aspect of the structure of invagination (Figure 1).

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