scholarly journals Nanoscale magnetic imaging of ferritins in a single cell

2019 ◽  
Vol 5 (4) ◽  
pp. eaau8038 ◽  
Author(s):  
Pengfei Wang ◽  
Sanyou Chen ◽  
Maosen Guo ◽  
Shijie Peng ◽  
Mengqi Wang ◽  
...  
Keyword(s):  
Single Cell ◽  
Imaging Techniques ◽  
Nitrogen Vacancy ◽  
Nanometer Scale ◽  
Magnetic Imaging ◽  
Intracellular Proteins ◽  
Nv Center ◽  
A Cell ◽  
Endogenous Proteins

The in situ measurement of the distribution of biomolecules inside a cell is one of the important goals in life science. Among various imaging techniques, magnetic imaging (MI) based on the nitrogen-vacancy (NV) center in diamond provides a powerful tool for the biomolecular research, while the nanometer-scale MI of intracellular proteins remains a challenge. Here, we use ferritin as a demonstration to realize the MI of endogenous proteins in a single cell using the NV center as the sensor. With the scanning, intracellular ferritins are imaged with a spatial resolution of ca. 10 nm, and ferritin-containing organelles are colocalized by correlative MI and electron microscopy. The approach paves the way for nanoscale MI of intracellular proteins.

scholarly journals Development of an in-situ SEOP 3 He Neutron Spin Filter for Magnetic Imaging Techniques

2017 ◽  
Vol 88 ◽  
pp. 231-236 ◽  
Author(s):  
H. Hayashida ◽  
K. Hiroi ◽  
T. Oku ◽  
H. Kira ◽  
K. Sakai ◽  
...  
Keyword(s):  
Imaging Techniques ◽  
Neutron Spin ◽  
Spin Filter ◽  
Magnetic Imaging

scholarly journals Quantum magnetic imaging of iron organelles within the pigeon cochlea

2021 ◽  
Vol 118 (47) ◽  
pp. e2112749118
Author(s):  
Robert W. de Gille ◽  
Julia M. McCoey ◽  
Liam T. Hall ◽  
Jean-Philippe Tetienne ◽  
E. Pascal Malkemper ◽  
...  
Keyword(s):  
Magnetic Susceptibility ◽  
Magnetic Particle ◽  
Imaging Techniques ◽  
Iron Storage ◽  
Magnetic Imaging ◽  
Geomagnetic Fields ◽  
The Individual ◽  
Individual Particles

The ability of pigeons to sense geomagnetic fields has been conclusively established despite a notable lack of determination of the underlying biophysical mechanisms. Quasi-spherical iron organelles previously termed “cuticulosomes” in the cochlea of pigeons have potential relevance to magnetoreception due to their location and iron composition; however, data regarding the magnetic susceptibility of these structures are currently limited. Here quantum magnetic imaging techniques are applied to characterize the magnetic properties of individual iron cuticulosomes in situ. The stray magnetic fields emanating from cuticulosomes are mapped and compared to a detailed analytical model to provide an estimate of the magnetic susceptibility of the individual particles. The images reveal the presence of superparamagnetic and ferrimagnetic domains within individual cuticulosomes and magnetic susceptibilities within the range 0.029 to 0.22. These results provide insights into the elusive physiological roles of cuticulosomes. The susceptibilities measured are not consistent with a torque-based model of magnetoreception, placing iron storage and stereocilia stabilization as the two leading putative cuticulosome functions. This work establishes quantum magnetic imaging as an important tool to complement the existing array of techniques used to screen for potential magnetic particle–based magnetoreceptor candidates.

scholarly journals Nitrogen-vacancy centers in diamond for nanoscale magnetic resonance imaging applications

2019 ◽  
Vol 10 ◽  
pp. 2128-2151 ◽  
Author(s):  
Alberto Boretti ◽  
Lorenzo Rosa ◽  
Jonathan Blackledge ◽  
Stefania Castelletto
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
High Resolution ◽  
Point Defect ◽  
Nitrogen Vacancy ◽  
Resonance Imaging ◽  
Magnetic Imaging ◽  
Nv Center ◽  
Nitrogen Vacancy Centers ◽  
Nv Centers

The nitrogen-vacancy (NV) center is a point defect in diamond with unique properties for use in ultra-sensitive, high-resolution magnetometry. One of the most interesting and challenging applications is nanoscale magnetic resonance imaging (nano-MRI). While many review papers have covered other NV centers in diamond applications, there is no survey targeting the specific development of nano-MRI devices based on NV centers in diamond. Several different nano-MRI methods based on NV centers have been proposed with the goal of improving the spatial and temporal resolution, but without any coordinated effort. After summarizing the main NV magnetic imaging methods, this review presents a survey of the latest advances in NV center nano-MRI.

scholarly journals High-throughput single-cell gene-expression profiling with multiplexed error-robust fluorescence in situ hybridization

2016 ◽  
Vol 113 (39) ◽  
pp. 11046-11051 ◽  
Author(s):  
Jeffrey R. Moffitt ◽  
Junjie Hao ◽  
Guiping Wang ◽  
Kok Hao Chen ◽  
Hazen P. Babcock ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Single Cell ◽  
Single Molecule ◽  
Rna Profiling ◽  
Drastic Increase ◽  
Cell Gene Expression ◽  
A Cell ◽  
Labeling Approach

Image-based approaches to single-cell transcriptomics, in which RNA species are identified and counted in situ via imaging, have emerged as a powerful complement to single-cell methods based on RNA sequencing of dissociated cells. These image-based approaches naturally preserve the native spatial context of RNAs within a cell and the organization of cells within tissue, which are important for addressing many biological questions. However, the throughput of these image-based approaches is relatively low. Here we report advances that lead to a drastic increase in the measurement throughput of multiplexed error-robust fluorescence in situ hybridization (MERFISH), an image-based approach to single-cell transcriptomics. In MERFISH, RNAs are identified via a combinatorial labeling approach that encodes RNA species with error-robust barcodes followed by sequential rounds of single-molecule fluorescence in situ hybridization (smFISH) to read out these barcodes. Here we increase the throughput of MERFISH by two orders of magnitude through a combination of improvements, including using chemical cleavage instead of photobleaching to remove fluorescent signals between consecutive rounds of smFISH imaging, increasing the imaging field of view, and using multicolor imaging. With these improvements, we performed RNA profiling in more than 100,000 human cells, with as many as 40,000 cells measured in a single 18-h measurement. This throughput should substantially extend the range of biological questions that can be addressed by MERFISH.

Coherent electron nanodiffraction from clean silver nano particles in a UHV STEM

1993 ◽  
Vol 51 ◽  
pp. 1058-1059
Author(s):  
J. Liu ◽  
M. Pan ◽  
G. E. Spinnler
Keyword(s):  
Supported Catalysts ◽  
Imaging Techniques ◽  
Nano Particles ◽  
Metal Particles ◽  
Shape Structure ◽  
Dynamical Simulations ◽  
Small Metal Particles ◽  
Extract Information

Small metal particles have peculiar chemical and physical properties as compared to bulk materials. They are especially important in catalysis since metal particles are common constituents of supported catalysts. The structural characterization of small particles is of primary importance for the understanding of structure-catalytic activity relationships. The shape and size of metal particles larger than approximately 5 nm in diameter can be determined by several imaging techniques. It is difficult, however, to deduce the shape of smaller metal particles. Coherent electron nanodiffraction (CEND) patterns from nano particles contain information about the particle size, shape, structure and defects etc. As part of an on-going program of STEM characterization of supported catalysts we report some preliminary results of CEND study of Ag nano particles, deposited in situ in a UHV STEM instrument, and compare the experimental results with full dynamical simulations in order to extract information about the shape of Ag nano particles.

In-Situ Dual Beam (FIBSEM) Techniques for Probe Pad Deposition and Dielectric Integrity Inspection in 0.2 μm Technology DRAM Single Cells

1999 ◽  
Author(s):  
Gunnar Zimmermann ◽  
Richard Chapman
Keyword(s):  
Electron Beam ◽  
Single Cell ◽  
Single Cells ◽  
Ion Beam ◽  
Gate Oxide ◽  
Ion Milling ◽  
Dual Beam ◽  
Sem Imaging ◽  
Innovative Techniques

Abstract Dual beam FIBSEM systems invite the use of innovative techniques to localize IC fails both electrically and physically. For electrical localization, we present a quick and reliable in-situ FIBSEM technique to deposit probe pads with very low parasitic leakage (Ipara < 4E-11A at 3V). The probe pads were Pt, deposited with ion beam assistance, on top of highly insulating SiOx, deposited with electron beam assistance. The buried plate (n-Band), p-well, wordline and bitline of a failing and a good 0.2 μm technology DRAM single cell were contacted. Both cells shared the same wordline for direct comparison of cell characteristics. Through this technique we electrically isolated the fail to a single cell by detecting leakage between the polysilicon wordline gate and the cell diffusion. For physical localization, we present a completely in-situ FIBSEM technique that combines ion milling, XeF2 staining and SEM imaging. With this technique, the electrically isolated fail was found to be a hole in the gate oxide at the bad cell.

scholarly journals Microfluidic extraction and stretching of chromosomal DNA from single cell nuclei for DNA fluorescence in situ hybridization

2012 ◽  
Vol 14 (3) ◽  
pp. 443-451 ◽  
Author(s):  
Xiaozhu Wang ◽  
Shin-ichiro Takebayashi ◽  
Evans Bernardin ◽  
David M. Gilbert ◽  
Ravindran Chella ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Single Cell ◽  
Cell Nuclei ◽  
Chromosomal Dna

scholarly journals Sensing Electrochemical Signals Using a Nitrogen-Vacancy Center in Diamond

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 358
Author(s):  
Hossein T. Dinani ◽  
Enrique Muñoz ◽  
Jeronimo R. Maze
Keyword(s):  
Electric Field ◽  
Electron Spin ◽  
Electrolyte Solution ◽  
High Sensitivity ◽  
Theoretical Work ◽  
Coherence Time ◽  
Nitrogen Vacancy ◽  
Local Concentration ◽  
Concentration Of Ions ◽  
Nv Center

Chemical sensors with high sensitivity that can be used under extreme conditions and can be miniaturized are of high interest in science and industry. The nitrogen-vacancy (NV) center in diamond is an ideal candidate as a nanosensor due to the long coherence time of its electron spin and its optical accessibility. In this theoretical work, we propose the use of an NV center to detect electrochemical signals emerging from an electrolyte solution, thus obtaining a concentration sensor. For this purpose, we propose the use of the inhomogeneous dephasing rate of the electron spin of the NV center (1/T2★) as a signal. We show that for a range of mean ionic concentrations in the bulk of the electrolyte solution, the electric field fluctuations produced by the diffusional fluctuations in the local concentration of ions result in dephasing rates that can be inferred from free induction decay measurements. Moreover, we show that for a range of concentrations, the electric field generated at the position of the NV center can be used to estimate the concentration of ions.

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