scholarly journals Outbreak of CTX-M-15-Producing Enterotoxigenic Escherichia coli O159:H20 in the Republic of Korea in 2016

2017 ◽  
Vol 61 (9) ◽  
Author(s):  
Jin Seok Kim ◽  
Jungsun Park ◽  
Eunkyung Shin ◽  
Soojin Kim ◽  
Sung Suck Oh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High School ◽  
Food Samples ◽  
Enterotoxigenic Escherichia Coli ◽  
Republic Of Korea ◽  
Third Generation ◽  
Content Type ◽  
The Republic ◽  
Third Generation Cephalosporins

ABSTRACT We investigated an outbreak of enterotoxigenic Escherichia coli (ETEC) O159:H20 associated with the consumption of a tossed-noodle dish in a high school in 2016. Thirty-three ETEC strains isolated from clinical and food samples were genetically indistinguishable. The outbreak strains were resistant to third-generation cephalosporins and harbored a bla CTX-M-15 gene on a 97-kb self-transferable IncK plasmid. This is the first outbreak caused by CTX-M-15-producing ETEC strains.

scholarly journals National Prevalence of Resistance to Third-Generation Cephalosporins in Escherichia coli Isolates from Layer Flocks in France

2013 ◽  
Vol 57 (12) ◽  
pp. 6351-6353 ◽  
Author(s):  
Claire Chauvin ◽  
Laetitia Le Devendec ◽  
Eric Jouy ◽  
Maena Le Cornec ◽  
Sylvie Francart ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Confidence Interval ◽  
Egg Production ◽  
Generation Cephalosporin ◽  
Gene Control ◽  
Third Generation ◽  
Content Type ◽  
E Coli ◽  
National Prevalence ◽  
Third Generation Cephalosporins

ABSTRACTResistance ofEscherichia colito third-generation cephalosporin (3GC) in fecal samples representative of French egg production was studied. The susceptibility to cefotaxime ofE. coliisolates obtained by culture on nonselective media was determined. Twenty-two nonsusceptible isolates were obtained (7.51%; 95% confidence interval, 4.49 to 10.54%), the majority of which came from young birds. Most isolates carried ablaCTX-M-1group gene, and a few carried ablaCMY-2-like gene. Control of 3GC resistance in laying hens is needed.

scholarly journals Characterization of Third-Generation-Cephalosporin-Resistant Shiga Toxin-Producing Strains of Escherichia coli O157:H7 in Japan

2015 ◽  
Vol 53 (9) ◽  
pp. 3035-3038 ◽  
Author(s):  
Ryuji Kawahara ◽  
Kazuko Seto ◽  
Masumi Taguchi ◽  
Chie Nakajima ◽  
Yuko Kumeda ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Generation Cephalosporin ◽  
Escherichia Coli O157 ◽  
Third Generation ◽  
Content Type ◽  
Resistant Strains ◽  
Third Generation Cephalosporins

We isolated Shiga toxin-producingEscherichia coliO157:H7 strains resistant to third-generation cephalosporins. The resistant strains harboredblaCMY-2, a plasmid-mediated AmpC β-lactamase. Genotyping of isolates revealed the possible spread of this problematic bacterium. Results suggested the importance of the investigation and surveillance of enterobacteria with plasmids harboringblaCMY-2.

scholarly journals Molecular Characterization of Extended-Spectrum-β-Lactamase-Producing and Plasmid-Mediated AmpC β-Lactamase-Producing Escherichia coli Isolated from Stray Dogs in South Korea

2012 ◽  
Vol 56 (5) ◽  
pp. 2705-2712 ◽  
Author(s):  
Migma Dorji Tamang ◽  
Hyang-Mi Nam ◽  
Geum-Chan Jang ◽  
Su-Ran Kim ◽  
Myung Hwa Chae ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Companion Animals ◽  
Republic Of Korea ◽  
Stray Dogs ◽  
Content Type ◽  
First Report ◽  
E Coli ◽  
Extended Spectrum ◽  
The Republic ◽  
Clonal Spread

ABSTRACTA total of 47 extended-spectrum-cephalosporin-resistantEscherichia colistrains isolated from stray dogs in 2006 and 2007 in the Republic of Korea were investigated using molecular methods. Extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase phenotypes were identified in 12 and 23E. coliisolates, respectively. All 12 ESBL-producing isolates carriedblaCTX-Mgenes. The most common CTX-M types were CTX-M-14 (n= 5) and CTX-M-24 (n= 3). Isolates producing CTX-M-3, CTX-M-55, CTX-M-27, and CTX-M-65 were also identified. Twenty-one of 23 AmpC β-lactamase-producing isolates were found to carryblaCMY-2genes. TEM-1 was associated with CTX-M and CMY-2 β-lactamases in 4 and 15 isolates, respectively. In addition toblaTEM-1, two isolates carriedblaDHA-1, and one of them cocarriedblaCMY-2. Both CTX-M and CMY-2 genes were located on large (40 to 170 kb) conjugative plasmids that contained the insertion sequence ISEcp1upstream of theblagenes. Only in the case of CTX-M genes was there an IS903sequence downstream of the gene. The spread of ESBLs and AmpC β-lactamases occurred via both horizontal gene transfer, accounting for much of the CTX-M gene dissemination, and clonal spread, accounting for CMY-2 gene dissemination. The horizontal dissemination ofblaCTX-MandblaCMY-2genes was mediated by IncF and IncI1-Iγ plasmids, respectively. The clonal spread ofblaCMY-2was driven mainly byE. colistrains of virulent phylogroup D lineage ST648. To our knowledge, this is the first report ofblaDHA-1inE. colistrains isolated from companion animals. This study also represents the first report of CMY-2 β-lactamase-producingE. coliisolates from dogs in the Republic of Korea.

scholarly journals Molecular Characterization of CTX-M β-Lactamase and Associated Addiction Systems in Escherichia coli Circulating among Cattle, Farm Workers, and the Farm Environment

2013 ◽  
Vol 79 (13) ◽  
pp. 3898-3905 ◽  
Author(s):  
Migma Dorji Tamang ◽  
Hyang-Mi Nam ◽  
Mamata Gurung ◽  
Geum-Chan Jang ◽  
Su-Ran Kim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Farm Workers ◽  
Host Cells ◽  
Conjugative Plasmid ◽  
Republic Of Korea ◽  
Cattle Farm ◽  
Content Type ◽  
E Coli ◽  
The Republic ◽  
M 14

ABSTRACTA total of 84 extended-spectrum β-lactamase (ESBL)-producingEscherichia coliisolates from cattle, farm workers, and the farm environment isolated from February to September 2008 in the Republic of Korea were investigated. All 84 ESBL-producing isolates carriedblaCTX-Mgenes that belonged to the CTX-M-1 (n= 35) or CTX-M-9 (n= 49) family. The most predominant CTX-M type identified was CTX-M-14 (n= 49), followed by CTX-M-32 (n= 26). TheblaCTX-Mgenes were identified most commonly inE. coliisolates from feces (n= 29), teats (n= 25), and milk (n= 14). AblaCTX-M-14gene was also detected in anE. coliisolate from a farmer's hand. Transfer of theblaCTX-Mgene from 60blaCTX-M-positiveE. coliisolates to the recipientE. coliJ53 strain by conjugation was demonstrated. Plasmid isolation fromblaCTX-M-positive transconjugants revealed a large (95- to 140-kb) conjugative plasmid. Almost all (82/84)blaCTX-Mgenes possessed an insertion sequence, ISEcp1, upstream of theblaCTX-Mgene. Only in the case of the CTX-M-14 genes was IS903downstream of the gene. TheblaCTX-Mgenes were associated with seven kinds of addiction systems. Among them,pndAC,hok-sok, andsrnBCwere the most frequently identified addiction systems in both wild strains and transconjugants. The spread ofblaCTX-Mgenes was attributed to both clonal expansion and horizontal dissemination. Our data suggest that a combination of multiple addiction systems in plasmids carryingblaCTX-Mgenes could contribute to their maintenance in the host cells. To our knowledge, theblaCTX-M-32gene has not previously been reported in animal isolates from the Republic of Korea.

Mariniflexile maritimum sp. nov., isolated from seawater of the South Sea in the Republic of Korea

2021 ◽  
Vol 71 (7) ◽  
Author(s):  
So-Ra Ko ◽  
Ve Van Le ◽  
Long Jin ◽  
Sang-Ah Lee ◽  
Chi-Yong Ahn ◽  
...  
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Republic Of Korea ◽  
Rrna Gene ◽  
The South ◽  
Content Type ◽  
Link Type ◽  
South Sea ◽  
The Republic

A novel Gram-stain-negative, rod-shaped, aerobic, non-motile bacterial strain, designated M5A1MT, was isolated from seawater collected from the South Sea of the Republic of Korea. Based on 16S rRNA gene sequence similarity, strain M5A1MT was closely related to Mariniflexile gromovii KMM 6038T (95.3 %), Mariniflexile fucanivorans SW5T (95.2 %), Mariniflexile soesokkakense RSSK-9T (95.1 %), Yeosuana aromativorans GW1-1T (94.6 %) and Confluentibacter lentus HJM-3T (94.6 %). Genome-based phylogenetic analyses revealed that strain M5A1MT formed a distinct cluster with the type strains of the genus Mariniflexile . The major cellular fatty acid constituents (>5 % of the total fatty acids) were iso-C15:0, anteiso-C15 : 0, iso-C15 : 0 3-OH, iso-C15 : 1 G, iso-C16:03-OH and iso-C17 : 0 3-OH. The respiratory quinone was identified as MK-6. The major polar lipids were phosphatidylethanolamine and one unidentified polar lipid. The genomic DNA G+C content of strain M5A1MT was determined to be 37.7 mol%. On the basis of its phenotypic, phylogenetic and chemotaxonomic characteristics, strain M5A1MT is considered to represent a novel species within the genus Mariniflexile , for which the name Mariniflexile maritimum sp. nov. is proposed. The type strain is M5A1MT (=KCTC 72895T=JCM 33982T).

scholarly journals A Multiepitope Fusion Antigen Elicits Neutralizing Antibodies against Enterotoxigenic Escherichia coli and Homologous Bovine Viral Diarrhea VirusIn Vitro

2013 ◽  
Vol 20 (7) ◽  
pp. 1076-1083 ◽  
Author(s):  
Emad A. Hashish ◽  
Chengxian Zhang ◽  
Xiaosai Ruan ◽  
David E. Knudsen ◽  
Christopher C. Chase ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Viral Infection ◽  
Neutralizing Antibodies ◽  
Cell Epitope ◽  
Enterotoxigenic Escherichia Coli ◽  
Bovine Viral Diarrhea ◽  
Content Type ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Fusion Antigen

ABSTRACTDiarrhea is one of the most important bovine diseases. EnterotoxigenicEscherichia coli(ETEC) and bovine viral diarrhea virus (BVDV) are the major causes of diarrhea in calves and cattle. ETEC expressing K99 (F5) fimbriae and heat-stable type Ia (STa) toxin are the leading bacteria causing calf diarrhea, and BVDV causes diarrhea and other clinical illnesses in cattle of all ages. It is reported that maternal immunization with K99 fimbrial antigens provides passive protection to calves against K99 fimbrial ETEC and that BVDV major structural protein E2 elicits antibodies neutralizing against BVDV viral infection. Vaccines inducing anti-K99 and anti-STa immunity would protect calves more effectively against ETEC diarrhea, and those also inducing anti-E2 neutralizing antibodies would protect calves and cattle against diarrhea caused by both ETEC and BVDV. In this study, we used the ETEC K99 major subunit FanC as a backbone, genetically embedded the STa toxoid STaP12Fand the most-antigenic B-cell epitope and T-cell epitope predicted from the BVDV E2 glycoprotein into FanC for the multivalent antigen FanC-STa-E2, and examined immunogenicity of this multivalent antigen to assess vaccine potential against bovine diarrhea. Mice intraperitoneally (i.p.) immunized with this multivalent antigen developed anti-K99, anti-STa, and anti-BVDV antibodies. Moreover, elicited antibodies showed neutralization activities, as they inhibited adherence of K99 fimbrialE. coli, neutralized STa toxin, and prevented homologous BVDV viral infectionin vitro. Results from this study suggest that this multiepitope fusion antigen can potentially be developed as a vaccine for broad protection against bovine diarrhea and that the multiepitope fusion strategy may be generally applied for multivalent vaccine development against heterogeneous pathogens.

scholarly journals Complete Genome Sequence of Enterotoxigenic Escherichia coli Podophage LL11

2019 ◽  
Vol 8 (32) ◽  
Author(s):  
Matthew Theodore ◽  
Lauren Lessor ◽  
Chandler O’Leary ◽  
Rohit Kongari ◽  
Jason Gill ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genome Sequence ◽  
Alternative Treatment ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Opportunistic Pathogen ◽  
Foodborne Illness ◽  
Enterotoxigenic Escherichia Coli ◽  
Content Type ◽  
Illness Study

Enterotoxigenic Escherichia coli (ETEC) is an opportunistic pathogen that commonly causes foodborne illness. Study of bacteriophages against this pathogen could be useful to develop alternative treatment approaches. Here, we present the complete genome sequence of LL11, a T7-like podophage that infects ETEC.

scholarly journals Complete Genome Sequence of Enterotoxigenic Escherichia coli Siphophage LL5

2019 ◽  
Vol 8 (27) ◽  
Author(s):  
Denish Piya ◽  
Lauren Lessor ◽  
Mei Liu ◽  
Jason J. Gill
Keyword(s):  
Escherichia Coli ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Enterotoxigenic Escherichia Coli ◽  
Content Type ◽  
Traveler’S Diarrhea ◽  
Traveler's Diarrhea

ABSTRACT Here, we describe the complete genome sequence of siphophage LL5. LL5 is a T1-like phage isolated against enterotoxigenic Escherichia coli, which causes traveler’s diarrhea. LL5 is included as a component phage in the commercial prebiotic product PreforPro.

scholarly journals Enterotoxigenic Escherichia coli Prevents Host NF-κB Activation by Targeting IκBα Polyubiquitination

2012 ◽  
Vol 80 (12) ◽  
pp. 4417-4425 ◽  
Author(s):  
Xiaogang Wang ◽  
Philip R. Hardwidge
Keyword(s):  
Escherichia Coli ◽  
Immune Responses ◽  
Diarrheal Disease ◽  
Innate Immune ◽  
Host Cells ◽  
Enterotoxigenic Escherichia Coli ◽  
Host Responses ◽  
Innate Immune Responses ◽  
Content Type ◽  
Heat Stable

ABSTRACTThe NF-κB pathway regulates innate immune responses to infection. NF-κB is activated after pathogen-associated molecular patterns are detected, leading to the induction of proinflammatory host responses. As a countermeasure, bacterial pathogens have evolved mechanisms to subvert NF-κB signaling. EnterotoxigenicEscherichia coli(ETEC) causes diarrheal disease and significant morbidity and mortality for humans in developing nations. The extent to which this important pathogen subverts innate immune responses by directly targeting the NF-κB pathway is an understudied topic. Here we report that ETEC secretes a heat-stable, proteinaceous factor that blocks NF-κB signaling normally induced by tumor necrosis factor (TNF), interleukin-1β, and flagellin. Pretreating intestinal epithelial cells with ETEC supernatant significantly blocked the degradation of the NF-κB inhibitor IκBα without affecting IκBα phosphorylation. Data from immunoprecipitation experiments suggest that the ETEC factor functions by preventing IκBα polyubiquitination. Inhibiting clathrin function blocked the activity of the secreted ETEC factor, suggesting that this yet-uncharacterized activity may utilize clathrin-dependent endocytosis to enter host cells. These data suggest that ETEC evades the host innate immune response by directly modulating NF-κB signaling.

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