Mechanism of Reduced Vancomycin Susceptibility Conferred bywalKMutation in Community-Acquired Methicillin-Resistant Staphylococcus aureus Strain MW2
2014 ◽
Vol 59
(2)
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pp. 1352-1355
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Keyword(s):
ABSTRACTPoint mutations with unclear molecular mechanisms are often associated with vancomycin resistance inStaphylococcus aureus. Here, we observed that thewalK(G223D) mutation caused decreased expression of genes associated with cell wall metabolism, decreased autolytic activity, thickened cell walls, and reduced vancomycin susceptibility. A phosphorylation assay showed that WalK (G223D) exhibited reduced autophosphorylation, which led to reduced phosphorylation of WalR. An electrophoretic mobility shift assay indicated that WalK (G223D)-phosphorylated WalR had a reduced capacity to bind to theatlApromoter.
2016 ◽
Vol 60
(6)
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pp. 3455-3461
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Keyword(s):
2014 ◽
Vol 58
(9)
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pp. 5024-5035
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Keyword(s):
2013 ◽
Vol 57
(11)
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pp. 5658-5664
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Keyword(s):
1998 ◽
Vol 18
(10)
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pp. 5852-5860
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