Pigment characterization of the giant colony-forming haptophyte Phaeocystis globosa in the Beibu Gulf reveals blooms of different origins

2021 ◽  
Author(s):  
Jin-Xiu Wang ◽  
Fan-Zhou Kong ◽  
Hui-Xia Geng ◽  
Yue Zhao ◽  
Wei-Bing Guan ◽  
...  
Keyword(s):  
High Performance ◽  
Large Scale ◽  
Economic Losses ◽  
Array Detector ◽  
Environmental Damage ◽  
Beibu Gulf ◽  
Similar Distribution ◽  
Phaeocystis Globosa ◽  
Different Origins

The giant colony-forming haptophyte Phaeocystis globosa has caused several large-scale blooms in the Beibu Gulf since 2011, but the distribution and dynamics of the blooms remained largely unknown. In this study, colonies of P. globosa , as well as membrane-concentrated phytoplankton samples, were collected during eight cruises from September 2016 to August 2017 in the Beibu Gulf. Pigments were analyzed by high performance liquid chromatography coupled with a diode-array detector (HPLC-DAD). The pigment 19'-hexanoyloxyfucoxanthin (hex-fuco), generally considered as a diagnostic pigment for Phaeocystis , was not detected in P. globosa colonies in Beibu Gulf, whereas 19'-butanoyloxyfucoxanthin (but-fuco) was found in all colony samples. Moreover, but-fuco in membrane-concentrated phytoplankton samples exhibited a similar distribution pattern to that of P. globosa colonies, suggesting that but-fuco provided the diagnostic pigment for bloom-forming P. globosa in the Beibu Gulf. Based on the distribution of but-fuco in different water masses in the region prior to the formation of intensive blooms, it’s suggested that P. globosa blooms in the Beibu Gulf could originate from two different sources. IMPORTANCE Phaeocystis globosa has formed intensive blooms in the South China Sea and even around the world, causing huge social economic losses and environmental damage. However, little is known about the formation mechanism and dynamics of P. globosa blooms. 19'-hexanoyloxyfucoxanthin (hex-fuco) is often used as the pigment proxy to estimate Phaeocystis biomass, while this is challenged by the giant colony-forming P. globosa in the Beibu Gulf which only containing 19'-butanoyloxyfucoxanthin (but-fuco) but not hex-fuco. Using but-fuco as a diagnostic pigment, we traced two different origins of P. globosa bloom in Beibu Gulf. This study clarified the development process of P. globosa blooms in the Beibu Gulf, which provided a basis for the early monitoring and prevention of the bloom.

scholarly journals Separation and HPLC Characterization of Active Natural Steroids in a Standardized Extract from the Serratula coronata Herb with Antiseborrheic Dermatitis Activity

2020 ◽  
Vol 17 (18) ◽  
pp. 6453
Author(s):  
Marta Napierała ◽  
Joanna Nawrot ◽  
Justyna Gornowicz-Porowska ◽  
Ewa Florek ◽  
Arletta Moroch ◽  
...  
Keyword(s):  
High Performance ◽  
Seborrheic Dermatitis ◽  
Array Detector ◽  
Diode Array Detector ◽  
Therapeutic Benefits ◽  
Serratula Coronata ◽  
Positive Effect ◽  
Active Natural

Phytoecdysteroids are natural compounds with therapeutic benefits in both humans and animals. The effectiveness of natural products with health potential is based on the activities and potencies of their active ingredients. In this study, dominant ecdysteroids—ajugasterone C, 20-hydroxyecdysone and polypodine B—from the Serratula coronata (S. coronata) herb were separated by column chromatography, identified by spectroscopic data and quantified by high-performance liquid chromatography with a diode array detector (HPLC-DAD). The obtained concentration of ecdysteroids (approximately 23%) in the S. coronatae herb extract enhances the possibility of their use in pharmaceutical and cosmetic products with high levels of phytoecdysteroids. Moreover, this study has shown a positive effect of ecdysteroids-containing cream on changes in quality of life and a beneficial effect in reducing the symptoms of seborrheic dermatitis. It has been demonstrated that the application of the cream with phytoecdysteroids resulted in a statistically significant alleviation of symptoms (p < 0.05), especially in terms of itching, pain or burning sensations in the affected areas in comparison to previous symptoms.

Characterization of phenolic compounds from inner bark of Betula pendula

Holzforschung ◽  
2012 ◽  
Vol 66 (2) ◽  
Author(s):  
Jaana Liimatainen ◽  
Maarit Karonen ◽  
Jari Sinkkonen ◽  
Marjo Helander ◽  
Juha-Pekka Salminen
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Betula Pendula ◽  
Silver Birch ◽  
Biologically Active ◽  
Array Detector ◽  
Ionization Mass ◽  
Inner Bark ◽  
First Time

Abstract A method has been developed for the characterization of biologically active silver birch (Betula pendula) inner bark phenolics based on high-performance liquid chromatography/diode array detector (HPLC-DAD)/electrospray ionization-mass spectrometry (ESI-MS). It was demonstrated that the inner bark contains high amounts of flavonoids, arylbutanoids, diarylheptanoids, simple phenolic compounds, phenolic acids, lignans, and procyanidins. Altogether, 30 individual compounds were characterized based on their ultraviolet (UV) and MS data. Structures of 22 compounds were confirmed by nuclear magnetic resonance (NMR) spectroscopy. In addition to previously reported phenolic compounds, 12 compounds were identified in silver birch inner bark for the first time; two of them are novel compounds: 3-β-glucopyranosyloxy-2-hydroxy-1-(4-hydroxy-3-methoxy-phenyl)-propan-1-one and 1,7-bis-(4-hydroxyphenyl)-3-heptanol 3-O-β-ap-iofuranosyl-(1→2)-β-glucopyranoside.

scholarly journals Chemical characterization of an Ipecac active germplasm bank – a Brazilian endangered medicinal species

2021 ◽  
Vol 10 (15) ◽  
Author(s):  
Nádia Elígia Nunes Pinto Paracampo ◽  
Luiz Ricardo Santos de Souza ◽  
Jéssica Caporal Almeida ◽  
Osmar Alves Lameira
Keyword(s):  
High Performance ◽  
Genetic Material ◽  
Chemical Characterization ◽  
Array Detector ◽  
Germplasm Bank ◽  
Medicinal Species ◽  
Breeding Programs ◽  
The City ◽  
Selection Of

The active germplasm bank (AGB) of Carapichea ipecacuanha (Brot.) L. Andersson at Embrapa Eastern Amazon, in the city of Belém, PA, was the first of its kind to be opened in Brazil for this endangered medicinal species and holds important accessions for agricultural and reproductive research, including the production of active principles. This study aimed to chemically characterize 42 accessions from that AGB by simultaneously quantifying cephaeline and emetine contents in roots using high-performance liquid chromatography with a diode-array detector (HPLC-DAD). Cephaeline concentrations ranged from ‘undetected’ to 1.76%, whereas emetine concentrations were found between 0.64% and 2.49%. The overall emetine/cephaeline ratio varied from 0.43 to 3.52. The differences among mean concentrations of alkaloids observed by comparing the Scott-Knott test at 5% probability suggest the chemical variation among the samples assessed. Therefore, it is concluded such chemical differences may favor the selection of genetic material for commercial purposes based on the production of emetine and/or cephaeline, likewise may contribute to breeding programs.

scholarly journals Optimization of an analytical HPLC-DAD method for detecting hydroxycinnamic acid derivatives from mixtures of Saussurea grandifolia and Taraxacum coreanum

2021 ◽  
Vol 64 (1) ◽  
Author(s):  
Hak-Dong Lee ◽  
Leo Adrianne Paje ◽  
Sullim Lee ◽  
Ki Sung Kang ◽  
Kyungki Hong ◽  
...  
Keyword(s):  
Analytical Method ◽  
High Performance ◽  
Large Scale ◽  
Gradient Elution ◽  
Hydroxycinnamic Acid ◽  
Array Detector ◽  
Accurate Identification ◽  
Accuracy Test ◽  
Hydroxycinnamic Acid Derivatives ◽  
Taraxacum Coreanum

AbstractAn analytical method was established to identify and quantify hydroxycinnamic acids, such as 1,5-dicaffeoylquinic acid (DCQA) and chicoric acid (CA), in mixtures of Saussurea grandifolia and Taraxacum coreanum (MST) by using reverse-phase high-performance liquid chromatography coupled with diode array detector (HPLC-DAD). Analyses were carried out by using an INNO C18 column with a gradient elution system, and different parameters were used to validate our optimized method. Results demonstrated limits of detection and quantification of 5.46 × 10–3 and 16.54 × 10–3 mg/mL for DCQA and 0.37 × 10–3 and 1.14 × 10–3 mg/mL for CA, respectively. The calibration curves for DCQA and CA showed good linearity over the concentration ranges of 0.025–0.4 and 0.00625–0.1 mg/mL, respectively, and both exhibited r2 = 1.0000. In the accuracy test, high recovery rates were obtained ranging from 101.16–104.18% for DCQA and 97.55–108.49% for CA, while the precision values were ≤ 1.00% for DCQA and ≤ 1.21% for CA. The values obtained from our analyses support the use of this analytical method for the accurate identification and quantification of DCQA and CA from MST. Our methodology could be used further to determine the content of hydroxycinnamic acid derivatives in routine analyses and large-scale extraction processes.

scholarly journals NeuroPM toolbox: integrating Molecular, Neuroimaging and Clinical data for Characterizing Neuropathological Progression and Individual Therapeutic Needs

2020 ◽  
Author(s):  
Yasser Iturria-Medina ◽  
Felix Carbonell ◽  
Atoussa Assadi ◽  
Quadri Adewale ◽  
Ahmed F. Khan ◽  
...  
Keyword(s):  
Open Access ◽  
High Performance ◽  
Large Scale ◽  
Synergistic Interactions ◽  
Academic Researchers ◽  
Cross Platform ◽  
Therapeutic Needs ◽  
User Friendly ◽  
Performance Computing

There is a critical need for a better multiscale and multifactorial understanding of neurological disorders, covering from genes to neuroimaging to clinical factors and treatments effects. Here we present NeuroPM-box, a cross-platform, user-friendly and open-access software for characterizing multiscale and multifactorial brain pathological mechanisms and identifying individual therapeutic needs. The implemented methods have been extensively tested and validated in the neurodegenerative context, but there is not restriction in the kind of disorders that can be analyzed. By using advanced analytic modeling of molecular, neuroimaging and/or cognitive/behavioral data, this framework allows multiple applications, including characterization of: (i) the series of sequential states (e.g. transcriptomic, imaging or clinical alterations) covering decades of disease progression, (ii) intra-brain spreading of pathological factors (e.g. amyloid and tau misfolded proteins), (iii) synergistic interactions between multiple brain biological factors (e.g. direct tau effects on vascular and structural properties), and (iv) biologically-defined patients stratification based on therapeutic needs (i.e. optimum treatments for each patient). All models outputs are biologically interpretable. A 4D-viewer allows visualization of spatiotemporal brain (dis)organization. Originally implemented in MATLAB, NeuroPM-box is compiled as standalone application for Windows, Linux and Mac environments: neuropm-lab.com/software. In a regular workstation, it can analyze over 150 subjects per day, reducing the need for using clusters or High-Performance Computing (HPC) for large-scale datasets. This open-access tool for academic researchers may significantly contribute to a better understanding of complex brain processes and to accelerating the implementation of Precision Medicine (PM) in neurology.

Characterization of Pigments from Malus domestica Leaves for Wool Dyeing

2021 ◽  
Vol 903 ◽  
pp. 63-68
Author(s):  
Valda Valkovska ◽  
Liāna Orola
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Array Detector ◽  
Diode Array ◽  
Phytochemical Screening ◽  
Diode Array Detector ◽  
Aluminium Sulphate ◽  
Quercetin Derivatives ◽  
Wool Dyeing

The study will present results of chemical analysis of mass spectrometry, evaluating the content of pigments in M. domestica leaves dyeing solution and in dyed woollen yarn nowadays. Yarn was dyed with fresh leaves of M. domestica tree. Before dyeing the yarn was treated with potassium aluminium sulphate mordant. Dyestuffs from the yarn were extracted in compliance with modified hydrochloric method of hydrolysis. Extracts were analysed by ultra high-performance liquid chromatography coupled with a diode array detector and mass spectrometry (UHPLC-DAD-MS) for identification of the pigments. Phytochemical screening of dyeing solution of the M. domestica leaves showed presence of dihydrochalcones (phloretin, phlorizin), flavan-3-ols (dimeric B type proanthocyanidin, catechin, epicatechin, gallocatehin, epigallocatechin), flavanols (quercetin derivatives: hyperoside, rutin, quercetrin), phenolic acids and other compounds.

scholarly journals RP-HPLC-DAD-MSn Analysis and Butyrylcholinesterase Inhibitory Activity of Barbacenia blanchetii Extracts

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000
Author(s):  
Jósquia S Barbosa ◽  
Verônica M Almeida ◽  
Rosilene M Marçal ◽  
Alexsandro Branco
Keyword(s):  
High Performance ◽  
Methanol Extract ◽  
Array Detector ◽  
Ionization Mass ◽  
Diode Array Detector ◽  
Rp Hplc ◽  
Dependent Form ◽  
Ellman’S Method ◽  
First Time

High-performance liquid chromatography (HPLC) coupled to a diode-array detector (DAD) and electrospray ionization mass spectrometry (MS) was applied for the separation and identification of phenolic compounds in the n-hexane, ethyl acetate and methanol crude extract of Barbacenia blanchetii. The MS, MSn and UV data together with chemosystematic evidence allowed the structural characterization of five compounds: tricin, chrysoeriol, epi-gallocatechin, kaempferol 3- O-glucoside and caffeoylquinic acid. At the same time, these extracts were evaluated against butyrylcholinesterase using Ellman's method. All extracts inhibited BuChE in a concentration-dependent form; however, the methanol extract showed a better effect that the other extracts. These compounds have been identified previously in the Velloziaceae family, but for the first time as constituents of B. blanchetii.

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