scholarly journals Immunogenicity of an Autogenous Streptococcus suis Bacterin in Preparturient Sows and Their Piglets in Relation to Protection after Weaning

2010 ◽  
Vol 17 (10) ◽  
pp. 1589-1597 ◽  
Author(s):  
Christoph Georg Baums ◽  
Christian Brüggemann ◽  
Christoph Kock ◽  
Andreas Beineke ◽  
Karl-Heinz Waldmann ◽  
...  
Keyword(s):  
Streptococcus Suis ◽  
Inhibitory Effect ◽  
Active Immunization ◽  
Enzyme Linked Immunosorbent Assay ◽  
Maternal Immunity ◽  
Humoral Immune ◽  
Specific Pathogen ◽  
Suckling Piglets ◽  
Specific Igg ◽  
Weaning Piglets

ABSTRACT Streptococcus suis is an important porcine pathogen causing meningitis and other invasive diseases in piglets of different ages. Application of S. suis serotype 2 bacterins to specific-pathogen-free (SPF) weaning piglets has been demonstrated to protect against the homologous serotype. However, autogenous S. suis bacterins are also applied to sows and suckling piglets in the field. Therefore, comparative evaluation of different bacterin immunization regimes, including sow vaccination, was performed in this study. The main objectives were to determine the immunogenicity of an S. suis bacterin in sows prepartum and its influence on active immunization of piglets. Experimental infection of 6- and 8-week-old weaning piglets was performed to elucidate protective efficacies. Humoral immune responses were investigated by an enzyme-linked immunosorbent assay (ELISA) measuring muramidase-released protein (MRP)-specific IgG titers and by opsonophagocytosis assays. Bacterin application elicited high MRP-specific IgG titers in the serum and colostrum of sows, as well as opsonizing antibodies. Piglets from vaccinated sows had significantly higher MRP-specific titers than respective piglets from nonvaccinated sows until 6 weeks postpartum. Vaccination of suckling piglets did not result in high MRP-specific titers nor in induction of opsonizing antibodies. Furthermore, neither vaccination of suckling nor of weaning piglets from immunized sows was associated with a prominent active immune response and protection at 8 weeks postpartum. However, protection was observed in respective 6-week-old weaning piglets, most likely because of protective maternal immunity. In conclusion, this study provides the first results suggesting protective passive maternal immunity for S. suis serotype 2 after bacterin vaccination of sows and a strong inhibitory effect on active immunization of suckling and weaning piglets, leading to highly susceptible growers.

Piglet performance and immunity is determined by the parity of both the birth dam and the rearing dam

2013 ◽  
Vol 53 (1) ◽  
pp. 46 ◽  
Author(s):  
Y. J. Miller ◽  
A. M. Collins ◽  
D. Emery ◽  
D. J. Begg ◽  
R. J. Smits ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Adaptive Immunity ◽  
Whole Blood ◽  
Disease Susceptibility ◽  
Immune Cell ◽  
Saline Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Humoral Immune ◽  
Pathogen Challenge ◽  
Specific Igg

Gilt progeny have lower weaning weights and higher post-weaning medication and mortality rates, indicating greater disease susceptibility, than do sow progeny. The present study aimed to identify explanatory innate or adaptive immunity differences between gilt and sow progeny and potential pre- or post-natal influences. Sixty-four dams were vaccinated twice pre-farrowing with tetanus toxoid (TT). Serum (pre-vaccination) and colostrum and/or milk samples were collected to determine concentrations of TT-specific immunoglobulin G (IgG), by using an enzyme-linked immunosorbent assay (ELISA). Piglets were removed from their birth dam before suckling and fostered (not to their birth dam) to form 16 gilt and 16 sow litters, with five gilt-born and five sow-born piglets per litter. Piglets were vaccinated at weaning (4 weeks old) with either TT or saline (control). Sera and whole blood were collected from three gilt-born and three sow-born piglets per litter at 2, 4 and 7 weeks of age. Innate immunity was assessed indirectly on whole blood using an interferon gamma (IFN-γ) immune cell stimulation assay and a phagocytic assay. Piglets were weighed at birth, 4, 10, 17 and 22 weeks of age. There was no difference (P > 0.05) in the concentration of TT-specific IgG in colostrum and milk from gilts and older-parity sows, suggesting a similar ability to transfer IgG antibodies to a novel antigen. Birth dam parity did not affect piglets’ TT-specific IgG concentrations pre-weaning (P > 0.05) suggesting similar ability to absorb passively acquired IgG. Sow-reared piglets, however, had lower (P < 0.05) concentrations of TT-specific IgG than did gilt-reared piglets, possibly due to haemodilution in the faster-growing sow progeny. Gilt-born progeny had a reduced IgG response post-weaning to TT vaccination relative to sow-born progeny (P < 0.05), indicating adaptive immunity differences. Birth dam parity did not affect (P > 0.05) innate immunity (number/responsiveness of cells). Rearing dam parity influenced phagocytic activity pre- and post-weaning (gilt-reared > sow-reared; P < 0.05), possibly due to increased pathogen challenge. Birthweight was affected by birth dam parity (sow-born > gilt-born; P < 0.05) while rearing dam parity determined weaning weight (sow-reared > gilt-reared; P < 0.05), with no difference evident at 22 weeks. The results of the present study suggest that gilt-born progeny may be more susceptible to disease post-weaning than sow-born progeny due to their lower birthweight and reduced humoral immune responsiveness. The rearing dam may also affect disease susceptibility in progeny due to slower pre-weaning growth, lower weaning weights and increased pathogen challenge, both pre- and post-weaning.

scholarly journals A Recombinant Turkey Herpesvirus Expressing F and HN Genes of Avian Avulavirus-1 (AAvV-1) Genotype VI Confers Cross-Protection against Challenge with Virulent AAvV-1 Genotypes IV and VII in Chickens

Viruses ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 784 ◽  
Author(s):  
Krzysztof Śmietanka ◽  
Jolanta Tyborowska ◽  
Monika Olszewska-Tomczyk ◽  
Katarzyna Domańska-Blicharz ◽  
Zenon Minta ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Vaccine Candidate ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Humoral Immune ◽  
Hemagglutination Inhibition Test ◽  
Challenge Study ◽  
Viral Vaccine ◽  
Specific Pathogen ◽  
Clinical Protection

Newcastle disease (ND) is responsible for significant economic losses in the poultry industry. The disease is caused by virulent strains of Avian avulavirus 1 (AAvV-1), a species within the family Paramyxoviridae. We developed a recombinant construct based on the herpesvirus of turkeys (HVT) as a vector expressing two genes: F and HN (HVT-NDV-F-HN) derived from the AAvV-1 genotype VI (“pigeon variant” of AAvV-1). This recombinant viral vaccine candidate was used to subcutaneously immunize one group of specific pathogen-free (SPF) chickens and two groups of broiler chickens (20 one-day-old birds/group). Humoral immune response was evaluated by hemagglutination-inhibition test and enzyme-linked immunosorbent assay (ELISA). The efficacy of the immunization was assessed in two separate challenge studies performed at 6 weeks of age with the use of virulent AAvV-1 strains representing heterologous genotypes IV and VII. The developed vaccine candidate elicited complete protection in SPF chickens since none of the birds became sick or died during the 2-week observation period. In the broiler groups, 90% and 100% clinical protection were achieved after challenges with AAvV-1 of IV and VII genotypes, respectively. We found no obvious relationship between antibody levels and protection assessed in broilers in the challenge study. The developed recombinant HVT-NDV-F-HN construct containing genes from a genotype VI AAvV-1 offers promising results as a potential vaccine candidate against ND in chickens.

scholarly journals Humoral and Cellular Immunogenicity of Zoster Vaccine within One Year after Herpes Zoster

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S414-S414
Author(s):  
Eunyoung Lee ◽  
June Young Chun ◽  
Kyoung-Ho Song ◽  
Pyeong Gyun Choe ◽  
Ji Whan Bang ◽  
...  
Keyword(s):  
Herpes Zoster ◽  
Geometric Mean ◽  
Enzyme Linked Immunosorbent Assay ◽  
Prior History ◽  
Zoster Vaccine ◽  
Varicella Zoster ◽  
Humoral Immune ◽  
History Of ◽  
Specific Igg ◽  
One Year

Abstract Background Herpes zoster vaccination is recommended to patients with a prior history of herpes zoster to prevent reactivation. However, the appropriate timing of vaccination is controversial. We compared immunogenicity of vaccine according to timing of vaccination after zoster illness. Methods In this prospective observational study, subjects were stratified into two groups by the vaccination timing since their zoster illness: 6–12 months (within-1 year group) vs. 1–5 years (after-1 year group). Blood samples were collected before and 6 weeks after vaccination of zoster vaccine live. Varicella-zoster virus (VZV)-specific IgG concentrations were measured by enzyme-linked immunosorbent assay. Interferon-gamma enzyme-linked immunosorbent spot (ELISPOT) assays were performed to assess VZV specific T-cell responses. Results A total of 59 patients (18 in the within-1 year group and 41 in the after-1 year group) were enrolled. Ages were not significantly different between groups. The baseline geometric mean titer (GMT) of VZV IgG was higher in the within-1 year group than in the after-1 year group (245.8 IU/mL vs. 124.9 IU/mL; P = 0.040). The geometric mean fold-rise (GMFR) of VZV IgG was lower in the within-1 year group than in the after-1 year group (1.42 vs. 2.46; P = 0.002). The GMT of spot forming cell (SFC) counts by ELISPOT at baseline and 6 weeks after vaccination were not significantly different between groups. The GMFRs of SFCs were also comparable. Conclusion Zoster vaccination within 1 year after zoster illness may have disadvantage in the aspect of humoral immune response (ClinicalTrials.gov number, NCT02704572). Disclosures All authors: No reported disclosures.

scholarly journals Helicobacter felis Infection Is Associated with Lymphoid Follicular Hyperplasia and Mild Gastritis but Normal Gastric Secretory Function in Cats

2000 ◽  
Vol 68 (2) ◽  
pp. 779-790 ◽  
Author(s):  
Kenneth W. Simpson ◽  
Dalit Strauss-Ayali ◽  
Eugenio Scanziani ◽  
Reinhard K. Straubinger ◽  
Patrick L. McDonough ◽  
...  
Keyword(s):  
Enzyme Linked Immunosorbent Assay ◽  
Gastric Disease ◽  
Secretory Function ◽  
Necrosis Factor Alpha ◽  
Follicular Hyperplasia ◽  
Helicobacter Felis ◽  
Humoral Immune ◽  
Reverse Transcription Pcr ◽  
Specific Pathogen ◽  
Somatostatin Immunoreactivity

ABSTRACT The relationship of Helicobacter felis, a bacterium observed in the stomachs of cats, to gastric disease is unclear. The objective of this study was to determine if H. felisinfection alters gastric histopathology, proinflammatory cytokine expression, and secretory function and evokes a humoral immune response in cats. Five specific-pathogen-free (SPF)Helicobacter-free cats were studied before and for 1 year after oral inoculation with H. felis (ATCC 49179). Four SPFH. felis-uninfected cats served as controls. The stomachs of all five H. felis-inoculated cats became colonized, as determined by urease activity, histopathology, PCR, culture, and transmission electron microscopy of serial gastric biopsies at 0, 3, 5, 8, and 12 months. Uninoculated cats remained Helicobacterfree. Lymphoid follicular hyperplasia, atrophy, and fibrosis were observed primarily in the pylorus of infected cats. Mild mononuclear inflammation was detected in both infected and uninfected cats, but was more extensive in infected cats, with pangastric inflammation, eosinophilic infiltrates, and cardia gastritis observed only in infected cats. No upregulation of antral mucosal interleukin 1α (IL-1α), IL-1β, or tumor necrosis factor alpha was detected by reverse transcription-PCR in any cat. The gastric secretory axes, assessed by fasting plasma gastrin, antral mucosal gastrin and somatostatin immunoreactivity, and pentagastrin-stimulated gastric acid secretion, were similar in both infected and uninfected cats. Gradual seroconversion (immunoglobulin G) was observed in four of five infected cats, with enzyme-linked immunosorbent assay values reaching 4× to 12× baseline 12 months postinfection. These findings indicate thatH. felis infection in cats induces lymphoid follicular hyperplasia, mild gastritis, and seroconversion, but is associated with normal gastric secretory function.

scholarly journals Colostral immunity of piglets to the Aujeszky disease virus in case of active immunization sows

2020 ◽  
Vol 8 (4) ◽  
pp. 257-260
Author(s):  
K. O. Holda ◽  
D. M. Masiuk ◽  
A. V. Kokariev ◽  
T. O. Vasilenko
Keyword(s):  
Disease Virus ◽  
Active Immunization ◽  
Vaccine Antigen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immune Protection ◽  
Specific Antibodies ◽  
Young Pigs ◽  
Aujeszky's Disease ◽  
Aujeszky’S Disease ◽  
Specific Igg

The current question of today is the formation of effective immune protection in young pigs against infectious diseases, achieved by piglets vaccination in the first weeks of their life. It is known that one of the factors influencing the quality of vaccination is colostral antibodies, which are able to deactivate the vaccine antigen. Considering this, it is important to determine the duration of colostral immunity of piglets to antigens of the Aujeszky’s disease virus during active sows immunization. For this, was formed a group of sows of 2nd-3rd gestation periods with 25 animals in each. Sows were immunized parenterally against Aujeszky’s disease with the «Adivak» vaccine at a dose of 2 ml, by mass vaccination, three times per year. The level of specific antibodies to glycoproteins E (gE) and B (gB) was determined in the blood serum of piglets before sucking colostrum and every 7 days of life until 77 days from birth by enzyme-linked immunosorbent assay (ELISA). It was revealed that before consuming colostrum, piglets did not have specific antibodies to the antigens of the Aujeszky disease virus. From 7th till 70th days of life, all piglets had specific IgG to the antigens gB and gE of the Aujeszky disease virus, and by the seventy-seventh day, 29% of the animals were seronegative. Thus, newborn piglets before colostrum suckling do not have specific immune protection against the Aujeszky disease virus against the background of sow immunization. Consumption of colostrum by piglets promotes their formation of colostral immunity, specific to the antigens of the Aujeszky disease virus. The duration of colostral antibodies’ circulation specific to the antigens of the Aujeszky disease virus in the piglets peripheral blood is 70 days. On the seventy-seventh day from birth, the level of colostral immunoglobulins decreased sharply, which contributed to the appearance of seronegative animals and an increase in their sensitivity to the action of an epizootic strain of the virus.

scholarly journals SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Mikail Dogan ◽  
Lina Kozhaya ◽  
Lindsey Placek ◽  
Courtney Gunter ◽  
Mesut Yigit ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Neutralizing Antibodies ◽  
Vaccine Development ◽  
High Specificity ◽  
Spike Protein ◽  
Humoral Immune ◽  
Specificity And Sensitivity ◽  
Wide Range ◽  
Specific Igg ◽  
Negative Controls

AbstractDevelopment of antibody protection during SARS-CoV-2 infection is a pressing question for public health and for vaccine development. We developed highly sensitive SARS-CoV-2-specific antibody and neutralization assays. SARS-CoV-2 Spike protein or Nucleocapsid protein specific IgG antibodies at titers more than 1:100,000 were detectable in all PCR+ subjects (n = 115) and were absent in the negative controls. Other isotype antibodies (IgA, IgG1-4) were also detected. SARS-CoV-2 neutralization was determined in COVID-19 and convalescent plasma at up to 10,000-fold dilution, using Spike protein pseudotyped lentiviruses, which were also blocked by neutralizing antibodies (NAbs). Hospitalized patients had up to 3000-fold higher antibody and neutralization titers compared to outpatients or convalescent plasma donors. Interestingly, some COVID-19 patients also possessed NAbs against SARS-CoV Spike protein pseudovirus. Together these results demonstrate the high specificity and sensitivity of our assays, which may impact understanding the quality or duration of the antibody response during COVID-19 and in determining the effectiveness of potential vaccines.

scholarly journals Characterization of the Humoral Immune Response to Chlamydia Outer Membrane Protein 2 in Chlamydial Infection

2003 ◽  
Vol 10 (1) ◽  
pp. 103-107 ◽  
Author(s):  
I. Portig ◽  
J. C. Goodall ◽  
R. L. Bailey ◽  
J. S. H. Gaston
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Chlamydial Infection ◽  
Chlamydia Pneumoniae ◽  
Specific Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Recent Infection ◽  
Humoral Immune ◽  
Immunodominant Antigen

ABSTRACT Detection of antibodies to an outer membrane protein 2 (OMP2) by enzyme-linked immunosorbent assay (ELISA) by using either the Chlamydia trachomatis- or the Chlamydia pneumoniae-specific protein was investigated. OMP2 is an immunodominant antigen giving rise to antibody responses in humans infected with different C. trachomatis serovars (A to C and D to K) or with C. pneumoniae, which could be detected by OMP2 ELISA. OMP2 ELISA is not species specific, but antibody titers were usually higher on the homologous protein. The sensitivity of this assay was high but varied according to the “gold standard” applied. Levels of antibody to C. pneumoniae OMP2 as detected by ELISA seem to return to background or near-background values within a shorter period of time compared to antibodies to C. pneumoniae detected by microimmunofluorescence (MIF), making it more likely that positive results in ELISA reflect recent infection. Thus, OMP2 ELISA has distinct advantages over MIF and commercially available ELISAs and might be a useful tool for the serodiagnosis of chlamydial infection.

scholarly journals Purified Hexameric Epstein-Barr Virus-Encoded BARF1 Protein for Measuring Anti-BARF1 Antibody Responses in Nasopharyngeal Carcinoma Patients

2010 ◽  
Vol 18 (2) ◽  
pp. 298-304 ◽  
Author(s):  
E. K. Hoebe ◽  
S. H. Hutajulu ◽  
J. van Beek ◽  
S. J. Stevens ◽  
D. K. Paramita ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Antibody Responses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Humoral Immune ◽  
Barr Virus ◽  
Humoral Immune Responses ◽  
Human Sera ◽  
Epstein Barr ◽  
Humoral Responses

ABSTRACTWHO type III nasopharyngeal carcinoma (NPC) is highly prevalent in Indonesia and 100% associated with Epstein-Barr virus (EBV). NPC tumor cells express viral proteins, including BARF1, which is secreted and is considered to have oncogenic and immune-modulating properties. Recently, we found conserved mutations in the BARF1 gene in NPC isolates. This study describes the expression and purification of NPC-derived BARF1 and analyzes humoral immune responses against prototype BARF1 (B95-8) and purified native hexameric BARF1 in sera of Indonesian NPC patients (n= 155) compared to healthy EBV-positive (n= 56) and EBV-negative (n= 16) individuals. BARF1 (B95-8) expressed inEscherichia coliand baculovirus, as well as BARF1-derived peptides, did not react with IgG or IgA antibodies in NPC. Purified native hexameric BARF1 protein isolated from culture medium was used in enzyme-linked immunosorbent assay (ELISA) and revealed relatively weak IgG and IgA responses in human sera, although it had strong antibody responses to other EBV proteins. Higher IgG reactivity was found in NPC patients (P= 0.015) than in regional Indonesian controls or EBV-negative individuals (P< 0.001). IgA responses to native BARF1 were marginal. NPC sera with the highest IgG responses to hexameric BARF1 in ELISA showed detectable reactivity with denatured BARF1 by immunoblotting. In conclusion, BARF1 has low immunogenicity for humoral responses and requires native conformation for antibody binding. The presence of antibodies against native BARF1 in the blood of NPC patients provides evidence that the protein is expressed and secreted as a hexameric protein in NPC patients.

TLR4 regulates rabies virus-induced humoral immunity through recruitment of cDC2 to lymph organs

2021 ◽  
Author(s):  
Chen Chen ◽  
Chengguang Zhang ◽  
Haoqi Li ◽  
Zongmei Wang ◽  
Yueming Yuan ◽  
...  
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Rabies Virus ◽  
Humoral Immunity ◽  
Humoral Immune Response ◽  
Critical Role ◽  
Wild Type ◽  
Humoral Immune ◽  
Specific Igg ◽  
Molecular Patterns

Rabies, caused by rabies virus (RABV), is fatal to both humans and animals around the world. Effective clinical therapy for rabies has not been achieved, and vaccination is the most effective means of preventing and controlling rabies. Although different vaccines, such as live attenuated and inactivated vaccines, can induce different immune responses, different expression of pattern recognition receptors (PRRs) also causes diverse immune responses. Toll-like receptor 4 (TLR4) is a pivotal PRR that induces cytokine production and bridges innate and adaptive immunity. Importantly, TLR4 recognizes various virus-derived pathogen-associated molecular patterns (PAMPs) and virus-induced damage-associated molecular patterns (DAMPs), usually leading to the activation of immune cells. However, the role of TLR4 in the humoral immune response induced by RABV has not been revealed yet. Based on TLR4-deficient ( TLR4 -/- ) and wild-type (WT) mouse models, we report that TLR4-dependent recruitment of the conventional type-2 dendritic cells (CD8α - CD11b + cDC2) into secondary lymph organs (SLOs) is critical for antigen presentation. cDC2-initiated differentiation of Tfh cells promotes the proliferation of germinal centre (GC) B cells, the formation of GCs, and the production of plasma cells (PCs), all of which contribute to the production of RABV-specific IgG and virus-neutralizing antibodies (VNAs). Collectively, our work demonstrates that TLR4 is necessary for the recruitment of cDC2 and for the induction of RABV-induced humoral immunity, which is regulated by the cDC2-Tfh-GC B axis. IMPORTANCE Vaccination is the most efficient method to prevent rabies. TLR4, a well-known immune sensor, plays a critical role in initiating innate immune response. Here, we found that TLR4 deficiency ( TLR4 -/- ) mice suppressed the induction of humoral immune response after immunization with rabies virus (RABV), including reduced production of VNAs and RABV-specific IgG, compared with that occurred in wild-type (WT) mice. As a consequence, TLR4 -/- mice exhibited higher mortality than WT mice after challenge with virulent RABV. Importantly, further investigation found that TLR4 signaling promoted the recruitment of cDC2 (CD8α + CD11b - ), a subset of cDCs known to induce CD4 + T cell immunity through their MHC-II presentation machinery. Our results imply that TLR4 is indispensable for an efficient humoral response to rabies vaccine, which provides new insight into the development of novel rabies vaccines.

Expression and immunological evaluation of elongation factor Tu of Streptococcus suis serotype 2

2017 ◽  
Vol 20 (2) ◽  
pp. 277-284 ◽  
Author(s):  
X.J. Xia ◽  
L. Wang ◽  
L.K. Cheng ◽  
Z.Q. Shen ◽  
S.G. Li ◽  
...  
Keyword(s):  
Recombinant Expression ◽  
Elongation Factor ◽  
Streptococcus Suis ◽  
Elongation Factor Tu ◽  
Host Interactions ◽  
Humoral Immune ◽  
Humoral Immune Responses ◽  
Antigenic Proteins ◽  
Recombinant Expression Plasmid ◽  
Related Proteins

Abstract Streptococcus suis serotype 2 (SS2) is considered as a major pathogen that causes sepsis and meningitis in piglets and humans, but knowledge of its antigenic proteins remains limited so far. The surface-related proteins of pathogens often play significant roles in bacterium-host interactions and infection. Here, we obtained the elongation factor Tu (EF-Tu) gene of Streptococcus suis and constructed the recombinant expression plasmid successfully. The target recombinant plasmid was then expressed in Escherichia coli and the immuno-protection of the recombinant protein was subsequently evaluated as well. The EF-Tu gene of Streptococcus suis is 1197 bp in length, encodes 398 amino acids. The target recombinant EF-Tu (rEF-Tu) protein can recognize the antiserum of Streptococcus suis and can provoke obvious humoral immune responses in rabbits and conferred protection to rabbits against Streptococcus suis ear-vein challenge, implying that the EF-Tu may be used as an attractive candidate antigen for a component of subunit vaccine.

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