scholarly journals Complete Genome Sequence of Bacillus velezensis CN026 Exhibiting Antagonistic Activity against Gram-Negative Foodborne Pathogens

2018 ◽  
Vol 6 (4) ◽  
Author(s):  
Catherine Nannan ◽  
Annika Gillis ◽  
Simon Caulier ◽  
Jacques Mahillon

ABSTRACT We report here the complete genome sequence of Bacillus velezensis strain CN026, a member of the B. subtilis group, which is known for its many industrial applications. The genome contains 3,995,812 bp and displays six gene clusters potentially involved in strain CN026’s activity against Gram-negative foodborne pathogens.

2019 ◽  
Vol 11 (12) ◽  
pp. 3529-3533
Author(s):  
Pavelas Sazinas ◽  
Morten Lindqvist Hansen ◽  
May Iren Aune ◽  
Marie Højmark Fischer ◽  
Lars Jelsbak

Abstract Many of the soil-dwelling Pseudomonas species are known to produce secondary metabolite compounds, which can have antagonistic activity against other microorganisms, including important plant pathogens. It is thus of importance to isolate new strains of Pseudomonas and discover novel or rare gene clusters encoding bioactive products. In an effort to accomplish this, we have isolated a bioactive Pseudomonas strain DTU12.1 from leaf-covered soil in Denmark. Following genome sequencing with Illumina and Oxford Nanopore technologies, we generated a complete genome sequence with the length of 5,943,629 base pairs. The DTU12.1 strain contained a complete gene cluster for a rare thioquinolobactin siderophore, which was previously described as possessing bioactivity against oomycetes and several fungal species. We placed the DTU12.1 strain within Pseudomonas gessardii subgroup of fluorescent pseudomonads, where it formed a distinct clade with other Pseudomonas strains, most of which also contained a complete thioquinolobactin gene cluster. Only two other Pseudomonas strains were found to contain the gene cluster, though they were present in a different phylogenetic clade and were missing a transcriptional regulator of the whole cluster. We show that having the complete genome sequence and establishing phylogenetic relationships with other strains can enable us to start evaluating the distribution and evolutionary origins of secondary metabolite clusters.


2017 ◽  
Vol 5 (48) ◽  
Author(s):  
Pingping Sun ◽  
Jianchao Cui ◽  
Xiaohui Jia ◽  
Wenhui Wang

ABSTRACT Bacillus velezensis L-1 is an effective biocontrol agent against pear diseases. Here, we report the complete genome sequence of B. velezensis L-1 in which clusters related to the biosynthesis of secondary metabolites were predicted. This genome provides insights into the possible biocontrol mechanisms and furthers application of this specific bacterium.


2018 ◽  
Vol 6 (14) ◽  
Author(s):  
M. Fatima C. Ilagan-Cruzada ◽  
Albert Remus R. Rosana ◽  
Andrew D. Montecillo ◽  
Noel G. Sabino ◽  
Ida F. Dalmacio

ABSTRACT Lactobacillus plantarum subsp. plantarum strain LB1-2, isolated from the hindgut of European honeybees in the Philippines, is active against Paenibacillus larvae and has broad activity against several Gram-positive and Gram-negative bacteria. The complete genome sequence reported herein contains gene clusters for multiple bacteriocins and extensive gene inventories for carbohydrate metabolism.


2019 ◽  
Vol 8 (17) ◽  
Author(s):  
Jacob Chamblee ◽  
Chi Zeng ◽  
Chandler J. O'Leary ◽  
Jason J. Gill ◽  
Mei Liu

Salmonella enterica serovar Enteritidis is a Gram-negative bacterium and one of the most common foodborne pathogens. Biocontrol using bacteriophage in food products or animals is one possible means by which pathogenic salmonellosis infection could be inhibited.


2017 ◽  
Vol 5 (35) ◽  
Author(s):  
Jinjin Ma ◽  
Hu Liu ◽  
Kai Liu ◽  
Chengqiang Wang ◽  
Yuhuan Li ◽  
...  

ABSTRACT Bacillus velezensis GQJK49 is a plant growth-promoting rhizobacterium with antifungal activity, which was isolated from Lycium barbarum L. rhizosphere. Here, we report the complete genome sequence of B. velezensis GQJK49. Twelve gene clusters related to its biosynthesis of secondary metabolites, including antifungal and antibacterial antibiotics, were predicted.


2019 ◽  
Author(s):  
Youquan Li ◽  
Xuan Li ◽  
Dan Jia ◽  
Junlong Liu ◽  
Jinming Wang ◽  
...  

AbstractBacillus velezensis JT3-1 is a probiotic strain isolated from feces of the domestic yak (Bos grunniens) in the Gansu province of China. It has strong antagonistic activity against Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Salmonella Typhimurium, Mannheimia haemolytica, Staphylococcus hominis, Clostridium perfringens, and Mycoplasma bovis. These properties have made the JT3-1 strain the focus of commercial interest. In this study, we describe the complete genome sequence of JT3-1, with a genome size of 3,929,799 bp, 3761 encoded genes and an average GC content of 46.50%. Whole genome sequencing of Bacillus velezensis JT3-1 will lay a good foundation for elucidation of the mechanisms of its antimicrobial activity, and for its future application.


2017 ◽  
Vol 5 (24) ◽  
Author(s):  
Beibei Wang ◽  
Hu Liu ◽  
Hailin Ma ◽  
Chengqiang Wang ◽  
Kai Liu ◽  
...  

ABSTRACT Bacillus velezensis JTYP2 was isolated from the leaves of Echeveria laui in Qingzhou, China, and may control some of the fungal pathogens of the plant. Here, we present the complete genome sequence of B. velezensis JTYP2. Several gene clusters related to its biosynthesis of antimicrobial compounds were predicted.


2008 ◽  
Vol 191 (1) ◽  
pp. 447-448 ◽  
Author(s):  
David A. Baltrus ◽  
Manuel R. Amieva ◽  
Antonello Covacci ◽  
Todd M. Lowe ◽  
D. Scott Merrell ◽  
...  

ABSTRACT Helicobacter pylori is a gram-negative pathogen that colonizes the stomachs of over half the world's population and causes a spectrum of gastric diseases including gastritis, ulcers, and gastric carcinoma. The H. pylori species exhibits unusually high levels of genetic variation between strains. Here we announce the complete genome sequence of H. pylori strain G27, which has been used extensively in H. pylori research.


2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Zhonghe Guo ◽  
Honglan Huang ◽  
Xiaolin Wu ◽  
Yuchong Hao ◽  
Yanbo Sun

Acinetobacter baumannii is a Gram-negative opportunistic pathogen that is frequently associated with nosocomial infections. Bacteriophages infecting A. baumannii can be used as effective agents to control these infections. Here, we announce the complete genome sequence of the lytic bacteriophage LZ35 infecting A. baumannii isolates.


2020 ◽  
Vol 9 (24) ◽  
Author(s):  
Man-Seok Bang ◽  
Hee-Won Jeong ◽  
Yea-Jin Lee ◽  
Sang-Cheol Lee ◽  
Gi Soo Lee ◽  
...  

ABSTRACT In the present work, we report the complete genome sequence of Bacillus velezensis DKU_NT_04, isolated from cheonggukjang, which is a traditional Korean fermented soybean paste. The final genome assembly consists of a 4.328-Mbp chromosome with 4,134 coding sequences and a G+C content of 45.21%.


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