Comparative Study of Cultured Burkitt Tumor Cells by Immunofluorescence, Autoradiography, and Electron Microscopy

Purpose. To compare the capsule edges ultrastructure obtained by two femtosecond laser-assisted cataract surgery (FLACS) platforms and manual continuous curvilinear capsulorhexis (CCC) using scanning electron microscopy (SEM). Setting. Eye Clinic, University of Trieste, Italy. Design. Experimental comparative study. Methods. 150 anterior capsules were collected and divided into three groups as follows: Group 1 (50 capsules) obtained with manual CCC, Groups 2 and 3 (each with 50 capsules) obtained with the Catalys Laser and the LenSx Laser, respectively. All samples were imaged by means of SEM and regularity of the cut surface, and thickness of the capsule edge were evaluated and compared. Results. All femtosecond laser (FSL) capsules were perfectly circular, whereas some alteration of the circular shape was observed in the manual ones. Group 1 showed a smooth and regular capsule edge without any surface irregularity, conversely Groups 2 and 3 showed postage-stamp perforations on the capsule edge. The cut surface irregularity value in Group 2 was 1.4 ± 0.63, while it was 0.7 ± 0.49 in Group 3 (p<0.05). Group 1 had a significantly lower thickness of the capsule edge than the FSL groups (p<0.05). No statistically significant difference in the capsule edge thickness between the FSL groups was found (p=0.244). Conclusions. Despite the presence of slight cut surface irregularities, both FSL capsulotomies showed a better geometry and circularity than the manual ones. Capsulotomy specimens obtained using both FSL capsulotomies showed laser-induced alterations of the capsule edge when compared with smooth and regular edges obtained using manual CCC.

Download Full-text

Early stages of high-temperature oxidation of Ni- and Co-base model superalloys: A comparative study using rapid thermal annealing and advanced electron microscopy

Corrosion Science ◽

10.1016/j.corsci.2021.109744 ◽

2021 ◽

pp. 109744

Author(s):

Dorota Kubacka ◽

Martin Weiser ◽

Erdmann Spiecker

Keyword(s):

Electron Microscopy ◽

High Temperature ◽

Comparative Study ◽

Thermal Annealing ◽

Rapid Thermal Annealing ◽

High Temperature Oxidation ◽

Temperature Oxidation ◽

Early Stages

Download Full-text

Biosynthesis, characterization, and antibacterial activity of ZnO nanoaggregates using aqueous extract from Anacardium occidentale leaf: comparative study of different precursors

Beni-Suef University Journal of Basic and Applied Sciences ◽

10.1186/s43088-020-00091-7 ◽

2021 ◽

Vol 10 (1) ◽

Author(s):

Eric Kwabena Droepenu ◽

Ebenezer Aquisman Asare ◽

Boon Siong Wee ◽

Rafeah Binti Wahi ◽

Frederick Ayertey ◽

...

Keyword(s):

Electron Microscopy ◽

Antibacterial Activity ◽

Comparative Study ◽

Aqueous Extract ◽

Zinc Chloride ◽

Zinc Acetate ◽

Zinc Acetate Dihydrate ◽

Inhibition Zone ◽

Anacardium Occidentale ◽

Acetate Dihydrate

Abstract Background Various parts of Anacardium occidentale plant possess curative qualities like antidiabetic, anti-inflammatory, antibacterial, antifungal, and antioxidant. Aqueous extract of this plant leaf was used in biosynthesizing zinc oxide (ZnO) nanoaggregates using two precursors of zinc salt (zinc acetate dihydrate [Zn(CH3COO)2∙2H2O] and zinc chloride [ZnCl2]). The synthesized ZnO samples were used in a comparative study to investigate the antibacterial activity against selected Gram-positive and Gram-negative microbes [Staphylococcus aureus, Exiguobacterium aquaticum (Gram +ve) and Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii (Gram −ve)]. The synthesized ZnO nanoaggregates from the two precursors were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy-dispersive x-ray spectroscopy (EDX) techniques. Results Micrographs of SEM and TEM confirmed nanoparticles agglomerated into aggregates. While spherical nanoaggregates were identified in samples prepared from Zn(CH3COO)2∙2H2O, flake-like structures were identified in samples synthesized from ZnCl2. Particle size determined by TEM was 107.03 ± 1.54 nm and 206.58 ± 1.86 nm for zinc acetate dihydrate and zinc chloride precursors respectively. ZnO nanoaggregate synthesized using zinc acetate as precursor gave higher antibacterial activity than its counterpart, zinc chloride with K. pneumonia recording the highest inhibition zone of 2.08 ± 0.03 mm (67.53%) whereas S. aureus recorded the least inhibition zone of 1.06 ± 0.14 mm (34.75%) for ZnO nanoaggregate from zinc chloride precursor. Also, antibacterial activity increases with increasing concentration of the extract in general. However, A. baumannii, E. aquaticum, and K. pneumoniae did not follow the continuity trend with regards to the 250 ppm and 500 ppm concentrations. Conclusion Biosynthesis of ZnO nanoaggregates using aqueous extract of A. occidentale leaf from zinc acetate dihydrate and zinc chloride as precursors was successful with the formation of nanospheres and nanoflakes. The study suggested that A. occidentale sp. could be an alternative source for the production of ZnO nanoparticles and are efficient antibacterial compounds against both Gram +ve and Gram −ve microbes with its promising effect against infectious bacteria.

Download Full-text

Three-dimensional relationships between tumor cells and microcirculation with double cyanine immunolabeling, laser scanning confocal microscopy, and computer-assisted reconstruction: an alternative to cast corrosion preparations.

Journal of Histochemistry & Cytochemistry ◽

10.1177/42.5.7908912 ◽

1994 ◽

Vol 42 (5) ◽

pp. 681-686 ◽

Cited By ~ 27

Author(s):

V Rummelt ◽

L M Gardner ◽

R Folberg ◽

S Beck ◽

B Knosp ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Blood Vessels ◽

Tumor Cells ◽

Laser Scanning ◽

Three Dimensional ◽

Melanoma Cells ◽

Tumor Blood Vessels ◽

The Relationship ◽

Scanning Electron

The morphology of the microcirculation of uveal melanomas is a reliable market of tumor progression. Scanning electron microscopy of cast corrosion preparations can generate three-dimensional views of these vascular patterns, but this technique sacrifices the tumor parenchyma. Formalin-fixed wet tissue sections 100-150 microns thick from uveal melanomas were stained with the lectin Ulex europaeus agglutinin I (UEAI) and proliferating cell nuclear antigen (PCNA) to demonstrate simultaneously the tumor blood vessels and proliferating tumor cells. Indocarbocyanine (Cy3) was used as a fluorophore for UEAI and indodicarbocyanine (Cy5) was used for PCNA. Double labeled sections were examined with a laser scanning confocal microscope. Images of both stains were digitized at the same 5-microns intervals and each of the two images per interval was combined digitally to form one image. These combined images were visualized through voxel processing to study the relationship between melanoma cells expressing PCNA and various microcirculatory patterns. This technique produces images comparable to scanning electron microscopy of cast corrosion preparations while permitting simultaneous localization of melanoma cells expressing PCNA. The microcirculatory tree can be viewed from any perspective and the relationship between tumor cells and the tumor blood vessels can be studied concurrently in three dimensions. This technique is an alternative to cast corrosion preparations.

Download Full-text

A Comparative Study of Trichomes in Angophora Cav. And Eucalyptus L'hérit. - A Question of Homology

Australian Journal of Botany ◽

10.1071/bt9840561 ◽

1984 ◽

Vol 32 (5) ◽

pp. 561 ◽

Cited By ~ 14

Author(s):

PY Ladiges

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Comparative Study ◽

Light Microscopy ◽

Evolutionary Trends ◽

Oil Glands ◽

Ancestral Condition ◽

Scanning Electron

The trichomes of Angophora and Eucalyptus are illustrated from scanning electron microscopy and light microscopy, and evolutionary trends are discussed. Bristle glands of Angophora and Eucalyptus subgen. Blakella and Corymbia are emergent oil glands of varying lengths. Emergent oil glands occur in all other Eucalyptus subgenera but they are most conspicuous in Blakella, Corymbia and Angophora, in which they are characterized by four cap cells each ornamented with micropapillae. Hairs in Angophora are unique, being multicellular; they are also uniseriate and scattered on the epidermis. In contrast, hairs in Eucalyptus are simple extensions, short or long, of the cells on the sides of or the cap cells of the emergent oil glands, and they are not homologous with those of Angophora. Eucalyptus setosa (subgen. Blakella) and E. brockwayi (subgen. Symphyomyrtus) are two exceptions, having unicellular hairs on the epidermis, not associated with oil glands. It is suggested that this is an ancestral condition (or secondary reversal to it).

Download Full-text

Adrenal Cortical Adenoma With Adrenalin-Type Neurosecretory Granules Clinically Mimicking a Pheochromocytoma

Archives of Pathology & Laboratory Medicine ◽

10.5858/2002-126-1530-acawat ◽

2002 ◽

Vol 126 (12) ◽

pp. 1530-1533 ◽

Cited By ~ 2

Author(s):

Tomislav Ivsic ◽

Richard A. Komorowski ◽

Gary S. Sudakoff ◽

Stuart D. Wilson ◽

Milton W. Datta

Keyword(s):

Electron Microscopy ◽

Clinical Outcome ◽

Tumor Cells ◽

Clinical Features ◽

Adrenal Tumor ◽

Histologic Examination ◽

Adrenal Tumors ◽

Neurosecretory Granules ◽

Adrenal Cortical Adenoma ◽

Pathologic Features

Abstract Adrenal tumors often present with clinical features that are specific and unique to their endocrine metabolism. When these features are in conflict with the pathologic appearance of the tumor, there can be great consternation for both the pathologist and the surgeon. In the case reported herein, an adrenalectomy was performed for clinical features of pheochromocytoma that on gross and histologic examination had the pathologic features of an adrenal cortical adenoma. Electron microscopy subsequently revealed that the tumor cells contained adrenalin-type granules, explaining the clinical outcome. It is crucial for both the surgeon and the surgical pathologist to be aware of this possibility when the clinical and pathologic features of an adrenal tumor are not congruent.

Download Full-text

Comparative Study of Field Emission-Scanning Electron Microscopy and Atomic Force Microscopy to Assess Self-assembled Monolayer Coverage on Any Type of Substrate

Microscopy and Microanalysis ◽

10.1017/s1431927699990475 ◽

1999 ◽

Vol 5 (6) ◽

pp. 413-419 ◽

Cited By ~ 10

Author(s):

Bernardo R.A. Neves ◽

Michael E. Salmon ◽

Phillip E. Russell ◽

E. Barry Troughton

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Atomic Force Microscopy ◽

Comparative Study ◽

Field Emission ◽

Self Assembled Monolayer ◽

Force Microscopy ◽

Atomic Force ◽

Self Assembled ◽

Scanning Electron

Abstract: In this work, we show how field emission–scanning electron microscopy (FE-SEM) can be a useful tool for the study of self-assembled monolayer systems. We have carried out a comparative study using FE-SEM and atomic force microscopy (AFM) to assess the morphology and coverage of self-assembled monolayers (SAM) on different substrates. The results show that FE-SEM images present the same qualitative information obtained by AFM images when the SAM is deposited on a smooth substrate (e.g., mica). Further experiments with rough substrates (e.g., Al grains on glass) show that FE-SEM is capable of unambiguously identifying SAMs on any type of substrate, whereas AFM has significant difficulties in identifying SAMs on rough surfaces.

Download Full-text