Characterization of the promoter elements required for hepatic and intestinal transcription of the human apoB gene: definition of the DNA-binding site of a tissue-specific transcriptional factor.
1990 ◽
Vol 10
(6)
◽
pp. 2653-2659
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Keyword(s):
The promoter elements important for intestinal and hepatic transcription of the human apoB gene have been localized downstream of nucleotide -150. Footprinting analysis using hepatic nuclear extracts identified four protected regions, -124 to -100, -97 to -93, -86 to -33, and +33 to +52. Gel electrophoretic mobility shift assays showed that multiple factors interact with the apoB sequence -86 to -33, while the region -88 to -61 binds a single nuclear factor. Methylation interference analysis and nucleotide substitution mutagenesis identified the binding site of the factor between residues -78 and -68. Binding competition experiments indicate that this factor recognizes the regulatory elements of other liver-specific genes.
1990 ◽
Vol 10
(6)
◽
pp. 2653-2659
1997 ◽
Vol 11
(11)
◽
pp. 1651-1658
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2000 ◽
Vol 279
(1)
◽
pp. R99-R108
◽
1994 ◽
Vol 14
(9)
◽
pp. 5975-5985
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