The yeast UME5 gene regulates the stability of meiotic mRNAs in response to glucose

1994 ◽  
Vol 14 (5) ◽  
pp. 3446-3458
Author(s):  
R T Surosky ◽  
R Strich ◽  
R E Esposito
Keyword(s):  
Vegetative Growth ◽  
Signal Transduction Pathway ◽  
Kinase Domain ◽  
Specific Protein ◽  
Transcript Stability ◽  
The Family ◽  
Two Factors ◽  
Genetic Epistasis ◽  
The Stability ◽  
Acetate Medium

We reported previously that early meiotic transcripts are highly unstable. These mRNAs exhibit half-lives of approximately 3 min when expressed during vegetative growth in glucose medium and are stabilized twofold during sporulation in acetate medium. Two genes, UME2 and UME5, that regulate the stability of meiosis-specific transcripts have been identified. The wild-type UME5 gene, which has been analyzed in detail, decreases the stability of all meiotic mRNAs tested approximately twofold when expressed during vegetative growth but has no effect on the half-lives of a number of vegetative mRNAs examined. The UME5 gene is dispensable for mitotic and meiotic development. Cells in which the entire UME5 gene has been deleted are viable, although the generation time is slightly longer and sporulation is less efficient. The UME5 transcript is constitutively expressed, and its stability is not autoregulated. The UME5 gene encodes a predicted 63-kDa protein with homology to the family of CDC28 serine/threonine-specific protein kinases. The kinase activity appears to be central to the function of the UME5 protein, since alteration of a highly conserved amino acid in the kinase domain results in a phenotype identical to that of a ume5 deletion. Genetic epistasis studies suggest that the UME2 and UME5 gene products act in the same pathway to regulate meiotic transcript stability. This pathway is independent of deadenylation and translation, two factors known to be important in regulating mRNA turnover. Significantly, the UME5-mediated destabilization of meiotic mRNAs occurs in glucose- but not in acetate-containing medium. Thus, the UME5 gene appears to participate in a glucose signal transduction pathway governing message stability.

scholarly journals The yeast UME5 gene regulates the stability of meiotic mRNAs in response to glucose.

1994 ◽  
Vol 14 (5) ◽  
pp. 3446-3458 ◽  
Author(s):  
R T Surosky ◽  
R Strich ◽  
R E Esposito
Keyword(s):  
Vegetative Growth ◽  
Signal Transduction Pathway ◽  
Kinase Domain ◽  
Specific Protein ◽  
Transcript Stability ◽  
The Family ◽  
Two Factors ◽  
Genetic Epistasis ◽  
The Stability ◽  
Acetate Medium

We reported previously that early meiotic transcripts are highly unstable. These mRNAs exhibit half-lives of approximately 3 min when expressed during vegetative growth in glucose medium and are stabilized twofold during sporulation in acetate medium. Two genes, UME2 and UME5, that regulate the stability of meiosis-specific transcripts have been identified. The wild-type UME5 gene, which has been analyzed in detail, decreases the stability of all meiotic mRNAs tested approximately twofold when expressed during vegetative growth but has no effect on the half-lives of a number of vegetative mRNAs examined. The UME5 gene is dispensable for mitotic and meiotic development. Cells in which the entire UME5 gene has been deleted are viable, although the generation time is slightly longer and sporulation is less efficient. The UME5 transcript is constitutively expressed, and its stability is not autoregulated. The UME5 gene encodes a predicted 63-kDa protein with homology to the family of CDC28 serine/threonine-specific protein kinases. The kinase activity appears to be central to the function of the UME5 protein, since alteration of a highly conserved amino acid in the kinase domain results in a phenotype identical to that of a ume5 deletion. Genetic epistasis studies suggest that the UME2 and UME5 gene products act in the same pathway to regulate meiotic transcript stability. This pathway is independent of deadenylation and translation, two factors known to be important in regulating mRNA turnover. Significantly, the UME5-mediated destabilization of meiotic mRNAs occurs in glucose- but not in acetate-containing medium. Thus, the UME5 gene appears to participate in a glucose signal transduction pathway governing message stability.

Quality of Life (QOL) of Balinese Women Working in Spa Tourism Industry

2015 ◽  
Vol 1 (1) ◽  
pp. 13
Author(s):  
Putu Sucita Yanthy ◽  
Luh Gede Leli Kusuma Dewi ◽  
W. Citra Juwitasari
Keyword(s):  
Quality Of Life ◽  
Tourism Industry ◽  
Family Needs ◽  
Interesting Phenomenon ◽  
Tourist Destinations ◽  
Foreign Countries ◽  
The Family ◽  
Two Factors ◽  
Material Factor

Bali is one of spa tourist destinations having various categories of spas and spa treatments, and the most important is the spa therapists. Spa development becomes an interesting phenomenon to be studied when it is associated with an involvement of Balinese women as spa therapists in foreign countries. The world’s demand for Balinese spa therapists has become the motivation of women to work in this area. The work and life of Balinese spa therapists while they are working in foreign countries serve as parameters to know their quality of life, and these parameters are also the main focus of this study. Through in-depth interviews and questionnaires distributed to 20 therapists it was found out that 85 percent of them have revealed an improvement in their quality of life that is influenced by two factors: the material and intimacy factors. The material factor in question refers to the economic improvement of the family as they could earn enough income to cover their family needs. The intimacy factor in question refers to closeness and a sense of solidarity fostered while they are working abroad and the relationship within the family. This study concludes that the most important part of the development of spa in Bali is its female Balinese spa therapists due to the image that Balinese women working as spa therapists are loyal, hard-working and honest making them in demand among tourists who are seeking spa treatments. Being a spa therapist can improve their quality of life, which means that subjectively both material and intimacy factors are the aspects that affect the quality of life of the Balinese spa therapists.

A novel missense mutation of CRYBA1 in a northern Chinese family with inherited coronary cataract with blue punctate opacities

2021 ◽  
pp. 112067212110083
Author(s):  
Shu-Hua Ni ◽  
Juan-Mei Zhang ◽  
Jun Zhao
Keyword(s):  
Missense Mutation ◽  
High Throughput Sequencing ◽  
Bioinformatics Analysis ◽  
Genetic Defect ◽  
Kinase Domain ◽  
Chinese Family ◽  
Heterozygous Mutation ◽  
Scale Invariant ◽  
The Family ◽  
Northern Chinese

Purpose: To demonstrate the underlying genetic defect that contribute to inherited cataract in a northern Chinese pedigree. Methods: The study recruited a family pedigree with a diagnosis of bilateral coronary cataract with blue punctate opacities. Fourteen family members and 100 healthy volunteers were enrolled. DNA sample of the proband in this family were analyzed by high-throughput sequencing, which was then demonstrated by Sanger sequencing in the remained people in the family and 100 controls. The functional effect of mutant genes was investigated via bioinformatics analysis, including Polymorphism Phenotyping version2 (PolyPhen-2), Protein Variation Effect Analyzer (PROVEAN v1.1.3) Scale-Invariant Feature Transform (SIFT), and Mutation Taster. Results: In this three-generation family, a novel heterozygous mutation was found in the kinase domain of CRYBA1 gene (c.340C > T, p.R114C), which was only detected in patients in the family with inherited cataract and were not detected in the remained people in the family nor in normal people. The pathogenic effect of the mutation was verified via bioinformatics analysis. Conclusion: Our study presented the molecular experiments to confirm that a novel missense mutation of c.340 C > T located in exon 4 of CRYBA1 gene results in a bilateral coronary cataract with blue punctate opacities, which enriches the mutation spectrum of CRYBA1 gene in inherited cataract and deepens the understanding of the pathogenesis of inherited cataract.

scholarly journals INTRACISTRONIC MAPPING OF ELECTROPHORETIC SITES IN DROSOPHILA MELANOGASTER: FIDELITY OF INFORMATION TRANSFER BY GENE CONVERSION

Genetics ◽  
1974 ◽  
Vol 76 (2) ◽  
pp. 289-299
Author(s):  
Margaret McCarron ◽  
William Gelbart ◽  
Arthur Chovnick
Keyword(s):  
Drosophila Melanogaster ◽  
Information Transfer ◽  
Large Scale ◽  
Genetic Basis ◽  
Xanthine Dehydrogenase ◽  
Specific Protein ◽  
Wild Type ◽  
Electrophoretic Variation ◽  
The Stability ◽  
Recombination Experiments

ABSTRACT A convenient method is described for the intracistronic mapping of genetic sites responsible for electrophoretic variation of a specific protein in Drosophila melanogaster. A number of wild-type isoalleles of the rosy locus have been isolated which are associated with the production of electrophoretically distinguishable xanthine dehydrogenases. Large-scale recombination experiments were carried out involving null enzyme mutants induced on electrophoretically distinct wild-type isoalleles, the genetic basis for which is followed as a nonselective marker in the cross. Additionally, a large-scale recombination experiment was carried out involving null enzyme rosy mutants induced on the same wild-type isoallele. Examination of the electrophoretic character of crossover and convertant products recovered from the latter experiment revealed that all exhibited the same parental electrophoretic character. In addition to documenting the stability of the xanthine dehydrogenase electrophoretic character, this observation argues against a special mutagenesis hypothesis to explain conversions resulting from allele recombination studies.

scholarly journals Increasing kinase domain proximity promotes MST2 autophosphorylation during Hippo signaling

2020 ◽  
Vol 295 (47) ◽  
pp. 16166-16179
Author(s):  
Thao Tran ◽  
Jaba Mitra ◽  
Taekjip Ha ◽  
Jennifer M. Kavran
Keyword(s):  
Single Molecule ◽  
Hippo Pathway ◽  
Signal Propagation ◽  
Kinase Domain ◽  
Specific Protein ◽  
Hippo Signaling ◽  
Membrane Recruitment ◽  
Chemically Induced Dimerization ◽  
The Hippo Pathway

The Hippo pathway plays an important role in developmental biology, mediating organ size by controlling cell proliferation through the activity of a core kinase cassette. Multiple upstream events activate the pathway, but how each controls this core kinase cassette is not fully understood. Activation of the core kinase cassette begins with phosphorylation of the kinase MST1/2 (also known as STK3/4). Here, using a combination of in vitro biochemistry and cell-based assays, including chemically induced dimerization and single-molecule pulldown, we revealed that increasing the proximity of adjacent kinase domains, rather than formation of a specific protein assembly, is sufficient to trigger autophosphorylation. We validate this mechanism in cells and demonstrate that multiple events associated with the active pathway, including SARAH domain–mediated homodimerization, membrane recruitment, and complex formation with the effector protein SAV1, each increase the kinase domain proximity and autophosphorylation of MST2. Together, our results reveal that multiple and distinct upstream signals each utilize the same common molecular mechanism to stimulate MST2 autophosphorylation. This mechanism is likely conserved among MST2 homologs. Our work also highlights potential differences in Hippo signal propagation between each activating event owing to differences in the dynamics and regulation of each protein ensemble that triggers MST2 autophosphorylation and possible redundancy in activation.

Use of an Ambulatory Infusion Pump in a 12-Year-Old with Salmonella Osteomyelitis

DICP ◽  
1989 ◽  
Vol 23 (5) ◽  
pp. 379-381
Author(s):  
Allen J. Vaida ◽  
Chantel J. Mattiucci ◽  
Steven A. Shapiro ◽  
Linda A. Gusenko ◽  
Anna M. King
Keyword(s):  
Cost Saving ◽  
Antibiotic Therapy ◽  
Infusion Pump ◽  
Medication Administration ◽  
Sterile Water ◽  
Home Therapy ◽  
Parenteral Antibiotic ◽  
The Family ◽  
The Stability ◽  
Salmonella Osteomyelitis

A 12-year-old girl with sickle cell hemoglobinopathy presented with a Salmonella osteomyelitis of her right humerus requiring six weeks of parenteral antibiotic therapy. Home therapy was evaluated. Due to the frequency of the medication administration (every six hours) and the apprehension of the family members, a Pharmacia-Deltec CADD-VT Infusion Pump was chosen for drug administration. Based on the stability of ampicillin, 1.3 g q6h was administered to provide a minimum of 1 g for the last dose of a 24-hour cycle. Ampicillin 6 g contained in 100 mL of sterile water for injection provided a 60 mg/mL solution with an osmolarity of 347 mOsmol. The pump was programmed to deliver 22 mL of solution over one hour, every six hours. A keep-vein-open rate of 0.2 mL/h maintained line patency. A 100 mL cassette of solution prepared daily was replaced on the pump by a home therapy nurse each morning. At the end of six weeks of therapy, the osteomyelitis was eradicated. We found the use of an ambulatory infusion pump an effective, convenient, and cost-saving method of treatment for our patient.

scholarly journals Characterization of an activated human ros gene.

1986 ◽  
Vol 6 (9) ◽  
pp. 3109-3116 ◽  
Author(s):  
C Birchmeier ◽  
D Birnbaum ◽  
G Waitches ◽  
O Fasano ◽  
M Wigler
Keyword(s):  
Gene Transfer ◽  
Protein Kinases ◽  
Transmembrane Domain ◽  
Extracellular Domain ◽  
Kinase Domain ◽  
Specific Protein ◽  
Normal Human

A human oncogene, mcf3, previously detected by a combination of DNA-mediated gene transfer and a tumorigenicity assay, derives from a human homology of the avian v-ros oncogene. Both v-ros and mcf3 can encode a protein with homology to tyrosine-specific protein kinases, and both mcf3 and v-ros encode a potential transmembrane domain N terminal to the kinase domain. mcf3 probably arose during gene transfer from a normal human ros gene by the loss of a putative extracellular domain. There do not appear to be any other gross rearrangements in the structure of mcf3.

Separating the Contributions in Localization Methods: A Technique to Enhance Identification of Damping Related Parameters

1999 ◽  
Author(s):  
Hervé Algrain ◽  
Calogero Conti ◽  
Pierre Dehombreux
Keyword(s):  
Finite Element ◽  
Model Updating ◽  
Element Model ◽  
Dynamic Responses ◽  
Finite Element Model Updating ◽  
Global Localization ◽  
Localization Method ◽  
The Family ◽  
Family Based ◽  
The Stability

Abstract Finite Element Model Updating has for objective to increase the correlation between the experimental dynamic responses of a structure and the predictions from a model. Among different initial choices, these procedures need to establish a set of representative parameters to be updated in which some are in real error and some are not. It is therefore important to select the correct properties that have to be updated to ensure that no marginal corrections are introduced. In this paper the standard localization criteria are presented and a technique to separate the global localization criteria in family-based criteria for damped structures is introduced. The methods are analyzed and applied to both numerical and experimental examples; a clear enhancement of the results is noticed using the family-based criteria. A simple way to qualify the stability of a localization method to noise is presented.

The Authoritarian Spectrum Through the Prism of Individualism/Collectivism

2018 ◽  
Vol 11 (3) ◽  
pp. 47-68
Author(s):  
Ben Ahmed Hougua ◽  
El Amine Rachid ◽  
Siyouri Hind
Keyword(s):  
Professional Socialization ◽  
Main Idea ◽  
Categorical Variables ◽  
World Values Survey ◽  
Political Work ◽  
The Family ◽  
The Government ◽  
The Stability ◽  
The Relationship ◽  
General Configuration

The general orientation to authority in Moroccan society is associated with dynamics that characterize it at the basic social level. Respect for authority tends to take the form of a syndrome forged in the sphere of convergent styles of family, pedagogical, and professional socialization, before being expressed as political loyalty. This articulation is at the very heart of the theory of congruence developed by Harry Eickstein, and recently taken up by other political scientists. The main idea put forward by the proponents of this theory is that the stability of the government is brought to bear when the models of authority on which it rests enjoy social fascination. The purpose of this article is to study the relationship between respect for authority in the three spheres of social and political work based on World Values Survey (WVS) data on Moroccan society (N = 3651) including the family, workplace, and political world. The examination of the general configuration of the orientation to authority is parameterized according to the individualistic and collectivist profiles constructed in the WVS database, as categorical variables.

Stability Analysis for a Class of Linear Switched Positive Systems

2012 ◽  
Vol 562-564 ◽  
pp. 2084-2087
Author(s):  
Hui Ding ◽  
Xu Yang Lou
Keyword(s):  
Lyapunov Functions ◽  
Dwell Time ◽  
Positive Systems ◽  
Time Analysis ◽  
Arbitrary Switching ◽  
The Family ◽  
The Common ◽  
The Stability ◽  
Switched Positive Systems ◽  
Explicit Relation

This paper addresses stability properties of linear switched positive systems composed of continuous-time subsystems and discrete-time subsystems. Based on the common linear copositive Lyapunov functions, stability of the positive systems is discussed under arbitrary switching. Moreover, a sufficient condition on the minimum dwell time that guarantees the stability of linear switched positive systems. The dwell time analysis interprets the stability of linear switched positive systems through the distance between the eigenvector sets. Thus, an explicit relation in view of stability is obtained between the family of the involved subsystems and the set of admissible switching signals.

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