Mitotic behavior of centromeres in meiosis as the fertility restoration mechanism in wheat-rye amphihaploids

2014 ◽  
Vol 50 (8) ◽  
pp. 818-827 ◽  
Author(s):  
D. B. Loginova ◽  
O. G. Silkova
1971 ◽  
Vol 13 (1) ◽  
pp. 14-19 ◽  
Author(s):  
Ch. M. Tahir ◽  
K. Tsunewaki

Genes and chromosomes involved in fertility restoration mechanism in Triticum aestivum strain P168, an effective restorer for Aegilops ovata cytoplasm, were studied by conventional and monosomic analysis. The study revealed that chromosome 1C (or C-sat-2), which is a substitute for original chromosome 1D of Triticum aestivum var. erythrospermum, carries a dominant fertility-restoring gene, Rfc1. Chromosomes 2A, 2B, 2D, 4A, 5D and 7A of P168 were found to carry modifying genes which counteract the fertility-restoring gene, A possible homo-ancestral relationship of three fertility-restoring genes, Rf1, Rf2 and Rfc3, was suggested.


Author(s):  
Andrey ilinsky ◽  
Alexander Nefedov ◽  
Konstantin Evsenkin

Global climatic changes, technogenic pollution by pollutants, violations of technologies of exploitation of reclaimed land lead to a decrease in fertility and soil degradation of agricultural land. Adverse weather conditions, resulting in a lack of adequate flood water, and economic difficulties in agriculture make it difficult to fill the deficit of organic matter and macronutrients in reclaimed alluvial soils. The monitoring of agrochemical properties of alluvial meadow medium-loamy soil of the stationary site (reclaimed lands of JSC «Moskovskoye» of Ryazan region), located in the floodplain of the Oka river, conducted by the Meshchersky branch of Vniigim, showed the presence and intensification of degradation changes in the soil. Thus, comparing the agrochemical indicators in the layer 0–20 cm, carried out in 1995, with the indicators of 2019, it should be noted a decrease in soil fertility. The decrease in soil quality was expressed in a decrease in the amount of mobile phosphorus by 37.6 %, mobile potassium by 53.3 %. Also, during this time there was a decrease in organic matter by 9.1 %, and an increase in soil acidity was 0.6 pH. As a result of such changes, soils lose ecological stability and become more vulnerable to adverse weather and negative anthropogenic impacts. In such a situation, advanced agricultural techniques should be actively used to obtain guaranteed, environmentally safe crop yields and restore the fertility of degraded reclaimed soils. In this regard, there is a need to develop innovative methods of fertility restoration of degraded alluvial soils in reclaimed lands using multi-component organic-mineral ameliorants. Meshchersky branch performs research work in addressing this issue.


Author(s):  
Т.В. Никитина ◽  
А.А. Кашеварова ◽  
М.М. Гридина ◽  
А.А. Хабарова ◽  
А.Г. Мензоров ◽  
...  

Митотическая нестабильность кольцевых хромосом может приводить к появлению клеточных клонов с различной генетической структурой. В качестве модели нестабильности кольцевых хромосом в митозе мы использовали фибробласты от пациентов с r(8), r(13), r(18) и r(22) и полученные из них индуцированные плюрипотентные стволовые клетки (ИПСК). Линии ИПСК с r(22) имели относительно стабильный кариотип на протяжении десятков (до 60) пассажей и сохраняли неизменную структуру кольцевой хромосомы. Кариотип линий ИПСК с r(8) и r(18) на ранних пассажах стабильный, планируется его изучение на поздних пассажах. Наибольшее разнообразие кариотипа выявлено в линиях ИПСК с r(13), в которых наблюдали различные перестройки и выраженную клеточную гетерогенность. Определение факторов, влияющих на митотическую стабильность кольцевых хромосом, может иметь значение для консультирования пациентов. Mitotic instability of ring chromosomes can lead to the appearance of cell clones with different genetic structure. IPSCs from fibroblasts of patients with r(8), r(13), r(18), and r(22) were used as a model of ring chromosomes mitotic behavior. Karyotypes of iPSC lines with r(8) and r(18) have so far been evaluated only in the early passages, lines with r(22) have maintained a relatively stable karyotype up to 60 passages. The occurrence of rearrangements and cellular heterogeneity was found characteristic for r(13) iPSCs. The determination of factors affecting the ring chromosomes mitotic stability would be beneficial for the patient’s prognosis.


Confectionery sunflower - a special area of use of sunflower, which requires the creation of marketable seeds quality features. One of the possible ways to create large-fruited sunflower is to create production hybrids and lines. Objective: to evaluate the created new large-fruited sunflower lines by a complex of morphological characters and determine the best lines for use as large-seeds hybrids as parent components or source material. In 2016-2019 years on the basis of the Institute of Oilseed Crops NAAS a study was conducted to assess the economic characteristics of large-fruited sunflower lines. We studied a collection of 27 lines of large-seeds sources. The lines were created by direct selection or crossing and sampling: Reyny of Argentinean origin, Zaporizhzhya confectionery variety, confectionery hybrid with striped pericarp color of Israeli origin, white seed of Turkish origin, synthetic population - donor of complex resistance. To study from the collection, lines were drawn that went through at least 7 generations with selection for seed size. Experience has shown that the shortest growing season for lines 174d and KP11 was 99 days, and the longest for lines I2K670 was 109 days. In the studied collection, the greatest mass of 1000 seeds has the KP11-146.47g line, which is the mother component and does not have branching. The second by weight of 1000 seeds (109 g) stood out line 168v, which also had branches and pollen fertility restoration genes and will be used as the paternal form. The third largest is also one basket line ZKN51-100. The collection included lines originating from the same combination, but with a different morphotype for the presence and absence of branching. So, based on the combination of KP11 x Zaporizhzhya Confectionery, three lines were obtained. A mass of 1000 seeds was observed in 98-86 g, with the branching line having the largest mass of 1000 seeds. The lines created with one combination VK678 x ZKN32: with a branch 168a had a mass of 1000 seeds 95g, and a line 168b - without a branch 109 g. Of the two lines obtained from the descendants of the combination KP11 x the striped hybrid both had branches, but the seeds were much smaller (weight of 1000 seeds 59 and 79 g). The collection also studied samples created on the basis of varieties and populations 160c, 174, 175b, the mass of 1000 seeds of which turned out to be more acceptable for large-fruited use from 83 to 99 g. Summing up the results of studying the collection of newly created lines, we can highlight the lines 162d, 168v, 175b, KP11 that are potentially promising for use in hybrids. The selections showed that large-fruited lines can be obtained from large-fruited varieties, self-pollination of large-fruited hybrids and crossing lines with hybrids and varieties. Self-pollination and selection of large-fruited lines in several generations does not provide the necessary variability for positive changes in selections. The result of the selection by weight of 1000 seeds in the offspring from crosses and from populations creates opportunities for new large-seeds sunflower.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Konstantinos Pantos ◽  
Mara Simopoulou ◽  
Evangelos Maziotis ◽  
Anna Rapani ◽  
Sokratis Grigoriadis ◽  
...  

AbstractThe chronic nature of Chronic Endometritis (CE) along with the challenging management and infertility entailed, call for cutting-edge therapeutic approaches. This study introduces the novel treatment of intrauterine antibiotic infusion (IAI) combined with oral antibiotic administration (OAA), and it assesses respective performance against the gold standard treatment of OAA. Data sourced herein reports on treatment efficiency and fertility restoration for both patients aiming to conceive naturally or via In Vitro fertilization. Eighty CE patients, 40 presenting with recurrent implantation failure, and 40 with recurrent pregnancy loss, were enrolled in the IVF and the natural conception arm respectively. Treatment was subjected to randomization. Effectively treated patients proceeded with either a single IVF cycle or were invited to conceive naturally over a 6-month period. Combination of IAI and OAA provided a statistically significant enhanced effectiveness treatment rate (RR 1.40; 95%CI 1.07–1.82; p = 0.01). No statistically significant difference was observed regarding the side-effects rate (RR 1.33; 95%CI 0.80–2.22; p = 0.52). No statistically significant difference was observed for either arm regarding live-birth rate. Following an intention-to-treat analysis, employment of IAI corresponds to improved clinical pregnancy rate-albeit not reaching statistical significance. In conclusion, complimentary implementation of IAI could provide a statistically significant enhanced clinical treatment outcome.


Author(s):  
Gábor Bergmann

AbstractStudying large-scale collaborative systems engineering projects across teams with differing intellectual property clearances, or healthcare solutions where sensitive patient data needs to be partially shared, or similar multi-user information systems over databases, all boils down to a common mathematical framework. Updateable views (lenses) and more generally bidirectional transformations are abstractions to study the challenge of exchanging information between participants with different read access privileges. The view provided to each participant must be different due to access control or other limitations, yet also consistent in a certain sense, to enable collaboration towards common goals. A collaboration system must apply bidirectional synchronization to ensure that after a participant modifies their view, the views of other participants are updated so that they are consistent again. While bidirectional transformations (synchronizations) have been extensively studied, there are new challenges that are unique to the multidirectional case. If complex consistency constraints have to be maintained, synchronizations that work fine in isolation may not compose well. We demonstrate and characterize a failure mode of the emergent behaviour, where a consistency restoration mechanism undoes the work of other participants. On the other end of the spectrum, we study the case where synchronizations work especially well together: we characterize very well-behaved multidirectional transformations, a non-trivial generalization from the bidirectional case. For the former challenge, we introduce a novel concept of controllability, while for the latter one, we propose a novel formal notion of faithful decomposition. Additionally, the paper proposes several novel properties of multidirectional transformations.


Genetics ◽  
1996 ◽  
Vol 143 (3) ◽  
pp. 1383-1394
Author(s):  
Roger P Wise ◽  
Carren L Dill ◽  
Patrick S Schnable

Abstract Dominant alleles of the rf1 and rf2 nuclear-encoded fertility restorer genes are necessary for restoration of pollen fertility in T-cytoplasm maize. To further characterize fertility restoration mediated by the Rf1 allele, 123,500 gametes derived from plants carrying the Mutator transposable element family were screened for rf1-mutant alleles (rf1-m) Four heritable rf1-m alleles were recovered from these populations. Three rf1-m alleles were derived from the progenitor allele Rf1-IAl53 and one was derived from Rf1-Ky21. Cosegregation analysis revealed 5.5- and 2.4kb Mu1-hybridizing EcoRI restriction fragments in all of the male-sterile and none of the male-fertile plants in families segregating for rf1-m3207 and rf1-m3310, respectively. Mitochondrial RNA gel blot analyses indicated that all four rf1-m alleles in male-sterile plants cosegregated with the altered steady-state accumulation of 1.6 and O.6-kb T-urf13 transcripts, demonstrating that these transcripts are Rf1 dependent. Plants carrying a leaky mutant, rf1-m7323, revealed variable levels of Rf1-associated, T-urf13 transcripts and the degree of pollen fertility. The ability to obtain rf1-m derivatives from Rf1 indicates that Rf1 alleles produce a functional gene product necessary for the accumulation of specific T-urf13 transcripts in T-cytoplasm maize.


Genetics ◽  
1998 ◽  
Vol 150 (1) ◽  
pp. 383-391 ◽  
Author(s):  
Hoang V Tang ◽  
Ruying Chang ◽  
Daryl R Pring

Abstract Defective nuclear-cytoplasmic interactions leading to aberrant microgametogenesis in sorghum carrying the IS1112C male-sterile cytoplasm occur very late in pollen maturation. Amelioration of this condition, the restoration of pollen viability, involves a novel two-gene gametophytic system, wherein genes designated Rf3 and Rf4 are required for viability of individual gametes. Rf3 is tightly linked to, or represents, a single gene that regulates a transcript processing activity that cleaves transcriptsof orf107, a chimeric mitochondrial open reading frame specific to IS1112C. The mitochondrial gene urf 209 is also subject to nucleus-specific enhanced transcript processing, 5′ to the gene, conferred by a single dominant gene designated Mmt1. Examinations of transcript patterns in F2 and two backcross populations indicated cosegregation of the augmented orf107 and urf209 processing activities in IS1112C. Several sorghum lines that do not restore fertility or confer orf107 transcript processing do exhibit urf209 transcript processing, indicating that the activities are distinguishable. We conclude that the nuclear gene(s) conferring enhanced orf107 and urf209 processing activities are tightly linked in IS1112C. Alternatively, the similarity in apparent regulatory action of the genes may indicate allelic differences wherein the IS1112C Rf3 allele may differ from alleles of maintainer lines by the capability to regulate both orf107 and urf209 processing activities.


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