scholarly journals AB0062 TIME COURSE OF INTESTINAL PERMEABILITY AND BACTERIAL TRANSLOCATION IN THE MODEL OF ADJUVANT-INDUCED ARTHRITIS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 1062.2-1062
Author(s):  
S. Hecquet ◽  
P. Totoson ◽  
H. Martin ◽  
C. Peyronnel ◽  
M. Tournier ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Intestinal Permeability ◽  
Bacterial Translocation ◽  
Time Course ◽  
Intestinal Inflammation ◽  
Arthritis Score ◽  
Adjuvant Induced Arthritis ◽  
Tnf Α ◽  
Body Weights ◽  
And Control

Background:Intestinal inflammation, dysbiosis, intestinal permeability (IP) and bacterial translocation (BT) have been identified in patients with spondyloarthritis but the time at which they appear and their contribution to the pathogenesis of the disease is still a matter of debate.Objectives:To investigate the time-course of intestinal inflammation, IP and BT in a rat model of reactive arthritis, a subgroup of SpA, the adjuvant-induced arthritis model.Methods:Adjuvant-induced arthritis (AIA) was induced in 6-week-old male Lewis rats by an injection at the base of the tail of Mycobacterium butyricum with incomplete Freund’s adjuvant (Day (D) 0). Control rats received saline using the same procedure. Body weights and a clinical arthritis score were daily assessed. A group of AIA and control rats (n=15 per group) were euthanized at three different times of arthritis: D4 for the pre-arthritic phase (AIA-preclinical), D11 for the onset of arthritis (AIA-onset) and D28 for the acute phase (AIA-acute). In each group (AIA and control, n=15 per group)), IP was assessed by measuring plasma levels of zonulin (ELISA) and ileal mRNA expression of zonulin and occludin (RT-qPCR), BT was studied by measuring bacterial endotoxins (or LPS, by LCMS2 method), soluble CD-14 (sCD14, ELISA) and ileal mRNA expression of TLR-4, and intestinal inflammation was assessed by measuring ileal mRNA expression of IL-8, IL-33, IL-17, IL-23p19 and TNF-α (RT-qPCR). Joint damage was assessed by the determination of a clinical and radiographic score of hind paws.Results:Body weights of AIA rats decreased from D4 to D28 as compared to controls, in parallel to the development of a severe clinical and radiographic arthritic disease from D11 and D28. Compared to control rats, AIA induced an increase in plasma zonulin levels at D4, D11 but not at D28. Ileal mRNA zonulin overexpression occurred at D11 while occludin was unchanged. As early as Day 4 (preclinical phase), mRNA of IL-8, IL-33 and IL-17 were overexpressed in ileum from AIA. At Day 11 (onset), overexpression of IL-8 persisted and mRNA of TNF-α and IL-23p19 increased in AIA. Neither LPS levels nor ileal mRNA expression of TLR-4 were changed by arthritis whatever the phase of arthritis. By contrast, blood levels of sCD-14 was significantly increased in the AIA group at all stages of arthritis. No correlation was found between clinical and radiographic arthritis scores and zonulin or LPS levels. Conversely, a negative correlation was observed between intestinal IL-8 mRNA expression and arthritis score (r=-0.3, p=0.02).Conclusion:In an animal model of SpA, intestinal inflammation and increased intestinal permeability occur prior to joint inflammation, suggesting a role of these disorders in the pathogenesis of this disease.Acknowledgements:I would like to thank the Société Française de Rhumatologie for its support in this work.Disclosure of Interests:None declared

SAT0360 BACTERIAL TRANSLOCATION IN THE ADJUVANT INDUCED ARTHRITIS MODEL: PRELIMINARY STUDY AND IMPACT OF NSAIDS

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1127.1-1127
Author(s):  
S. Hecquet ◽  
R. Bordy ◽  
C. Prati ◽  
D. Wendling ◽  
C. Demougeot ◽  
...  
Keyword(s):  
Intestinal Permeability ◽  
Bacterial Translocation ◽  
Intestinal Inflammation ◽  
Vehicle Group ◽  
Control Group ◽  
Arthritis Score ◽  
Adjuvant Induced Arthritis ◽  
Radiographic Score ◽  
Tnf Α ◽  
Significant Difference

Background:In patients with spondyloarthritis, the presence of intestinal inflammation and an increase in digestive permeability responsible for bacterial translocation has been described. No data are available on the effect of non-steroidal anti-inflammatory drugs (NSAIDs) on this bacterial translocation in patients with spondyloarthritis. Zonulin and lipopolysaccharide (LPS) have been described as good biomarkers of intestinal permeability and bacterial translocation, respectively. Adjuvant-induced arthritis (AIA) is a model of recent arthritis characterized by ossification and ankylosis in the post arthritis period. This model can be considered as a model of reactive arthritis in which our previous work has reported a clear efficacy of NSAIDs with differences between molecules at the structural and vascular levels.Objectives:To test the hypothesis that there is an increase in digestive permeability and bacterial translocation in the AIA model and to show the influence of different NSAIDs on these two parameters.Methods:Adjuvant-induced arthritis (AIA) was induced in 6-week-old male Lewis rats by an injection at the base of the tail of Mycobacterium butyricum. A group of non-AIA (control) rats received saline. At the first signs of arthritis, the AIA-rats were evaluated (arthritis score 0-6) and treated daily intraperitoneally with naproxen (10 mg/kg/day), diclofenac (5mg/kg twice daily), celecoxib (3 mg/kg/day) or saline solution (AIA-vehicle group). After 21 days of treatment, the rats were sacrificed and serum levels of zonulin and LPS were evaluated by ELISA and liquid chromatography-mass spectrometry, respectively. Circulating levels of TNF-α and IL1-β and paw radiographic score were measured.Results:Compared to the control group, there was a significant increase in zonulin concentration (p < 0.001) in the AIA group. There was no significant difference in the concentration of LPS between the two groups. The levels of zonulin were correlated with the TNF-α levels (R= -0.42; p=0.032) and the arthritis score (R=0.45; p=0.013) but not with the level of IL1-β (R=; p-0.018; p=0.39). Treatment with NSAIDs significantly and equivalently decreased the arthritis score in each group. Compared to the vehicle group, treatment with naproxen significantly decreased the radiographic score (p<0.001), TNF-α, IL1-β (p < 0.01), zonulin (p<0.001) and LPS (p < 0.05). Celecoxib decreased radiographic score (p < 0.001), IL1-β (p < 0.01), TNF-α (p < 0.01) but increased zonulin levels (p < 0.05) without effect on LPS. Diclofenac also decreased radiographic score (p < 0.001), TNF-α (p < 0.01), and IL1-β (p < 0.01) but increased both zonulin (p < 0.01) and LPS (p < 0.001).Conclusion:We have demonstrated an increase in serum zonulin levels in the AIA model and a beneficial effect of naproxen on intestinal permeability and bacterial translocation in contrast to celecoxib and diclofenac. Moreover, the plasmatic zonulin levels were correlated with TNF-α supporting a pivotal role of TNF-α on the tight junctions in this model.Disclosure of Interests:Sophie Hecquet: None declared, Romain Bordy: None declared, Clément Prati: None declared, Daniel Wendling: None declared, Céline Demougeot Grant/research support from: With an institutional support from Pfizer., Frank Verhoeven: None declared

scholarly journals Inhibition of Intrahepatic Gamma Interferon Production by Hepatitis C Virus Nonstructural Protein 5A in Transgenic Mice

2009 ◽  
Vol 83 (17) ◽  
pp. 8463-8469 ◽  
Author(s):  
Tatsuo Kanda ◽  
Robert Steele ◽  
Ranjit Ray ◽  
Ratna B. Ray
Keyword(s):  
Transcription Factors ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Time Course ◽  
Nonstructural Protein ◽  
Gamma Interferon ◽  
Tnf Α ◽  
Ifn Γ ◽  
And Control ◽  
Nonstructural Protein 5A

ABSTRACT Hepatitis C virus (HCV) utilizes strategies to suppress or evade the host immune response for establishment of persistent infection. We have shown previously that HCV nonstructural protein 5A (NS5A) impairs tumor necrosis factor alpha (TNF-α)-mediated apoptosis. In this study, we have examined the immunomodulatory role of HCV NS5A protein in transgenic mouse (NS5A-Tg) liver when mice were challenged with an unrelated hepatotropic adenovirus as a nonspecific stimulus. Hepatotropic adenovirus was introduced intravenously into NS5A-Tg mice and control mice, and virus clearance from liver was compared over a time course of 3 weeks. The differential mRNA expression levels of 84 cytokine-related genes, signal pathway molecules, transcription factors, and cell surface molecules were determined using real-time reverse transcription-PCR array. NS5A-Tg mice failed to clear adenovirus from liver up to 3 weeks postinfection while control mice cleared virus within 1 to 2 weeks. Subsequent study revealed that gamma interferon (IFN-γ) expression is inhibited at both the mRNA and protein levels in NS5A-Tg mice, and an inverse expression of transcription factors Gata-3 and Tbx21 is observed. However, TNF-α mRNA and protein expression were elevated in both NS5A-Tg and control mice. Together, our results suggested that HCV NS5A acts as an immunomodulator by inhibiting IFN-γ production and may play an important role toward establishment of chronic HCV infection.

scholarly journals Cornelian Cherry Iridoid-Polyphenolic Extract Improves Mucosal Epithelial Barrier Integrity in Rat Experimental Colitis and Exerts Antimicrobial and Antiadhesive Activities In Vitro

2020 ◽  
Vol 2020 ◽  
pp. 1-19
Author(s):  
Marta Szandruk-Bender ◽  
Maria Rutkowska ◽  
Anna Merwid-Ląd ◽  
Benita Wiatrak ◽  
Adam Szeląg ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Intestinal Inflammation ◽  
Experimental Colitis ◽  
Epithelial Barrier ◽  
Trinitrobenzenesulfonic Acid ◽  
Cornelian Cherry ◽  
Polyphenolic Extract ◽  
Tnf Α ◽  
The Impact

Background and Aims. Inflammatory bowel disease pharmacotherapy, despite substantial progress, is still not satisfactory for both patients and clinicians. In view of the chronic and relapsing disease course and not always effective treatment with adverse effects, attempts to search for new, more efficient, and safer substances are essential and reasonable. This study was designed to elucidate the impact of cornelian cherry iridoid-polyphenolic extract (CE) and loganic acid (LA) on adherent-invasive E. coli growth and adhesion in vitro and to assess the effect of pretreatment with CE or LA on the course of intestinal inflammation in rat experimental colitis compared with sulfasalazine. Methods. Antibacterial and antiadhesive activities of CE and LA were assessed using microdilution, Int407 cell adherence, and yeast agglutination assays. The colitis model was induced by 2,4,6-trinitrobenzenesulfonic acid. Studied substances were administered intragastrically for 16 days prior to colitis induction. Body weight loss; colon index; histological injuries; IL-23, IL-17, TNF-α, and chemerin levels; and STAT3, Muc2, and TFF3 mRNA expression were evaluated. Results. Only CE exerted antimicrobial and antiadhesive activities in vitro and alleviated colonic symptoms. CE coadministrated with sulfasalazine was more effective than single compounds in reversing increased concentrations of TNF-α, IL-17, and chemerin and decreased Muc2 mRNA expression. Conclusions. CE exerted a protective effect against experimental colitis via impaired mucosal epithelial barrier restoration and intestinal inflammatory response attenuation and given concomitantly with sulfasalazine counteracted colitis in a more effective way than sulfasalazine alone, which indicates their synergistic interaction. The beneficial effect of CE may also be due to its bacteriostatic and antiadhesive activities.

scholarly journals Effects of dietary fiber sources during late gestation and lactation on sow performance, milk quality, and intestinal health in piglets1

2019 ◽  
Vol 97 (12) ◽  
pp. 4922-4933 ◽  
Author(s):  
Qinghui Shang ◽  
Hansuo Liu ◽  
Sujie Liu ◽  
Tengfei He ◽  
Xiangshu Piao
Keyword(s):  
Mrna Expression ◽  
Intestinal Inflammation ◽  
Immunoglobulin A ◽  
Interleukin 10 ◽  
Milk Quality ◽  
Late Gestation ◽  
Intestinal Barrier Function ◽  
Intestinal Health ◽  
Serum Growth Hormone ◽  
Tnf Α

Abstract This study was conducted to investigate the effects of dietary supplementation with 2 sources of fiber, sugar beet pulp (SBP), and wheat bran (WB), on sow performance, milk quality, and intestinal health in piglets. Forty-five multiparous sows at day 85 of gestation were allocated to the following 3 treatments: 1) a corn-soybean meal basal diet (CON); 2) the CON diet supplemented with 20% SBP in gestation and 10% SBP in lactation (SBP); and 3) the CON diet supplemented with 30% WB in gestation and 15% WB in lactation (WB). The SBP diets increased (P &lt; 0.05) sow ADFI during lactation, litter and piglet weaning weight, piglet ADG, immunoglobulin A (IgA), and interleukin-10 (IL-10) levels in the colostrum and IgA levels in the milk, while the WB diets only increased (P &lt; 0.05) IL-10 levels in the milk when compared with the CON diets. Piglets from SBP-fed sows had greater (P &lt; 0.05) serum growth hormone and insulin-like growth factor-1 levels than those from WB-fed or CON-fed sows, whereas piglets from WB-fed sows had greater (P &lt; 0.05) serum GH levels than those from CON-fed sows. Serum diamine oxidase activity, endotoxin, IL-6, and tumor necrosis factor-α (TNF-α) levels were reduced (P &lt; 0.05) in piglets from SBP-fed or WB-fed sows. Piglets from SBP-fed sows also had greater (P &lt; 0.05) serum IL-10 levels than those from CON-fed sows. The ileal mRNA expression of TNF-α was reduced (P &lt; 0.05) in piglets from SBP-fed or WB-fed sows. Piglets from SBP-fed sows had lower (P &lt; 0.05) IL-6 expression, and greater (P &lt; 0.05) IL-10 expression and secretory immunoglobulin A (SIgA) levels in the ileum than those from WB- or CON-fed sows. Piglets from WB-fed sows had greater (P &lt; 0.05) IL-10 expression and SIgA levels compared with those from CON-fed sows. The ileal mRNA expression of occludin in the ileum was greater (P &lt; 0.05) in piglets from SBP-fed sows than those from CON-fed sows. The ileal mRNA expression of ZO-1 was greater (P &lt; 0.05) in piglets from WB-fed sows than those from CON-fed sows, but lower (P &lt; 0.05) than those from SBP-fed sows. Piglets from SBP-fed sows had greater (P &lt; 0.05) abundance of Christensenellaceae and butyrate levels in the colon, while piglets from WB-fed sows had greater (P &lt; 0.05) abundance of Lactobacillaceae. Collectively, maternal SBP supplementation was more effective than WB in improving milk quality, enhancing growth performance and intestinal barrier function, and ameliorating intestinal inflammation in piglets.

scholarly journals Anethole Attenuates Enterotoxigenic Escherichia coli-Induced Intestinal Barrier Disruption and Intestinal Inflammation via Modification of TLR Signaling and Intestinal Microbiota

2021 ◽  
Vol 12 ◽  
Author(s):  
Qingyuan Yi ◽  
Jiaxin Liu ◽  
Yufeng Zhang ◽  
Hanzhen Qiao ◽  
Fang Chen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Performance ◽  
Mrna Expression ◽  
Intestinal Microbiota ◽  
Barrier Function ◽  
Intestinal Inflammation ◽  
Intestinal Barrier ◽  
Intestinal Barrier Function ◽  
Weaned Piglets ◽  
Tnf Α

This study aimed to investigate the effects of dietary anethole supplementation on the growth performance, intestinal barrier function, inflammatory response, and intestinal microbiota of piglets challenged with enterotoxigenic Escherichia coli K88. Thirty-six weaned piglets (24 ± 1 days old) were randomly allocated into four treatment groups: (1) sham challenge (CON); (2) Escherichia coli K88 challenge (ETEC); (3) Escherichia coli K88 challenge + antibiotics (ATB); and (4) Escherichia coli K88 challenge + anethole (AN). On day 12, the piglets in the ETEC, ATB, and AN group were challenged with 10 mL E. coli K88 (5 × 109 CFU/mL), whereas the piglets in the CON group were orally injected with 10 mL nutrient broth. On day 19, all the piglets were euthanized for sample collection. The results showed that the feed conversion ratio (FCR) was increased in the Escherichia coli K88-challenged piglets, which was reversed by the administration of antibiotics or anethole (P &lt; 0.05). The duodenum and jejunum of the piglets in ETEC group exhibited greater villous atrophy and intestinal morphology disruption than those of the piglets in CON, ATB, and AN groups (P &lt; 0.05). Administration of anethole protected intestinal barrier function and upregulated mucosal layer (mRNA expression of mucin-1 in the jejunum) and tight junction proteins (protein abundance of ZO-1 and Claudin-1 in the ileum) of the piglets challenged with Escherichia coli K88 (P &lt; 0.05). In addition, administration of antibiotics or anethole numerically reduced the plasma concentrations of IL-1β and TNF-α (P &lt; 0.1) and decreased the mRNA expression of TLR5, TLR9, MyD88, IL-1β, TNF-α, IL-6, and IL-10 in the jejunum of the piglets after challenge with Escherichia coli K88 (P &lt; 0.05). Dietary anethole supplementation enriched the abundance of beneficial flora in the intestines of the piglets. In summary, anethole can improve the growth performance of weaned piglets infected by ETEC through attenuating intestinal barrier disruption and intestinal inflammation.

scholarly journals Exogenous Autoinducer-2 Rescues Intestinal Dysbiosis and Intestinal Inflammation in a Neonatal Mouse Necrotizing Enterocolitis Model

2021 ◽  
Vol 11 ◽  
Author(s):  
Yan-Chun Ji ◽  
Qian Sun ◽  
Chun-Yan Fu ◽  
Xiang She ◽  
Xiao-Chen Liu ◽  
...  
Keyword(s):  
Necrotizing Enterocolitis ◽  
Intestinal Inflammation ◽  
Control Group ◽  
Inflammatory Factor ◽  
Formula Milk ◽  
Autoinducer 2 ◽  
Intestinal Dysbiosis ◽  
Tnf Α ◽  
And Control ◽  
Proinflammatory Factors

Autoinducer-2 (AI-2) is believed to be a bacterial interspecies signaling molecule that plays an important role in the regulation of the physiological behaviors of bacteria. The effect of AI-2 on the process of necrotizing enterocolitis (NEC) is unknown, and the aim of this study was to study the effect of AI-2 in a mouse NEC model. C57BL/6 mouse pups were randomly divided into three groups: the control group, the NEC group, and the NEC+AI-2 (NA) group. Exogenous AI-2 (500 nM) was added to the formula milk of the NA group. The concentrations of fecal AI-2 and flora were tested. The expression of cytokines, TLR4 and NF-κB in intestinal tissue was detected. The AI-2 level was significantly decreased in the NEC group (P&lt;0.05). Compared with the NEC group, the intestinal injury scores, expression of TLR4, NF-kB, and proinflammatory factors (IL-1β, IL-6, IL-8 and TNF-α) were reduced, and expression of anti-inflammatory factor (IL-10) was increased in the NA group mice (P&lt;0.05). At the phylum level, the Proteobacteria abundance in the NA group was significantly increased, while the Bacteroidota abundance in the control group was significantly increased (P&lt;0.05). At the genus level, Helicobacter and Clostridium_sensu_stricto_1 exhibited significantly greater abundance in the NEC group than in the other two groups, while Lactobacillus had the opposite trend (P&lt;0.05). In addition, the abundances of Klebsiella, Rodentibacter and Enterococcus were significantly higher in the NA group than in the NEC and control groups (P &lt; 0.05). Exogenous AI-2 partially reverses flora disorder and decreases inflammation in an NEC mouse model.

scholarly journals A23 DIETARY PROTEIN AND AMINO ACID COMPOSITIONS INFLUENCE MICROBIOTA, INTESTINAL PERMEABILITY, AND SUSCEPTIBILITY TO COLITIS

2021 ◽  
Vol 4 (Supplement_1) ◽  
pp. 266-268
Author(s):  
L Rondeau ◽  
J Godbout ◽  
X Wang ◽  
A CAMINERO FERNANDEZ
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Intestinal Permeability ◽  
Bacterial Translocation ◽  
Dietary Protein ◽  
Intestinal Inflammation ◽  
Clinical Signs ◽  
Fecal Microbiota ◽  
Ussing Chambers ◽  
Increased Risk

Abstract Background Environmental factors, such as alterations in diet and microbiota, have been linked to inflammatory bowel diseases (IBD). The incidence of IBD is rising, particularly in Canada and other industrialized nations that consume western-style diets high in fat and protein. While most dietary proteins and amino acids are absorbed in the small intestine, substantial amounts can enter the colon for microbial metabolism and to exert effects on intestinal tissue and immune cells. Prospective cohort studies suggest that diets high in protein are associated with an increased risk of IBD. However, the role of excess dietary protein and amino acids in IBD pathogenesis is not clear. Aims To study whether and how consumption of diets high in protein or amino acids influences intestinal inflammation, colitis severity, and intestinal microbiota. Methods To assess the influence of dietary protein composition on colitis severity, specific pathogen-free C57BL/6 mice were fed isocaloric casein-based purified diets containing low (7%), normal (14%), or high (35%) protein (HPD). Mice were also fed an amino acid-defined diet (AAD) with amino acid and ingredient composition matched to the normal protein diet. Following three weeks of diet consumption ad libitum, mice were continued on the same diet and mucosal injury was induced with 2% dextran sulfate sodium (DSS; 5 days) followed by water (2 days) before sacrifice. Mice were monitored daily for clinical signs of colitis. Susceptibility to colitis was assessed by analysing stool consistency and blood, microscopic scoring (Cooper score), and by immunohistochemistry of colon tissue. Fecal microbiota (16S rRNA Illumina), intestinal permeability (Ussing chambers), proinflammatory gene expression (NanoString and RT-qPCR), and bacterial translocation (plating) were analysed. Results Following DSS exposure, mice fed HPD and AAD experienced greater weight loss, bacterial translocation to the spleen, stool blood, and diarrhea compared to mice fed the normal protein control diet. While all DSS-treated mice developed colitis, HPD and AAD fed mice also developed greater histologic damage, intestinal permeability, and innate immune cell infiltration. Cytokine profiling revealed that AAD is associated with significant up-regulation of IL-18 during colitis. Principle coordinates analysis based on Bray-Curtis dissimilarities demonstrates distinct shifts in the fecal microbiota of mice fed HPD and AAD. Conclusions These results suggest that excess dietary protein and amino acids are associated with more severe colitis and microbiota alterations in the DSS model. Previous studies demonstrate that IL-18 is up-regulated in IBD patients. Its overexpression may incite inflammation by stimulating cytokine signalling through NFκB and modify microbial community structure by regulating antimicrobial peptides. Funding Agencies CIHRFarncombe Family Digestive Health Research Institute, Douglas Family Chair in Gastroenterology Research

scholarly journals The endogenous ligand for guanylate cyclase-C activation reliefs intestinal inflammation in the DSS colitis model

2020 ◽  
Author(s):  
Danfeng Lan ◽  
Yunling Wen ◽  
Xiangqian Dong ◽  
Qin Yang ◽  
Yan Liu ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Intestinal Permeability ◽  
Guanylate Cyclase ◽  
Intestinal Inflammation ◽  
The Body ◽  
Expression Level ◽  
Major Type ◽  
Inflammatory Injury ◽  
Guanylate Cyclase C ◽  
Tnf Α

Ulcerative colitis (UC) is a major type of inflammatory bowel disease (IBD) and significantly impacts patient quality of life. Previous research revealed that the guanylate cyclase-C (GC-C) signaling pathway is associated with the severity of UC. We aimed to investigate the effect of the GC-C agonist, guanylin (Gn), on inflammatory injury in mice with colitis. An experimental UC model was established in Balb/c mice. Mesalamine served as a positive control. The Gn overexpression vector was administered once per day for 1 week. Intestinal permeability of the mice was measured using fluorescein isothiocyanate-dextran after the treatment. Histopathologic grading was estimated to assess the inflammatory injury of the colon. The expression level of crucial mediators of the GC-C signaling pathway (Gn, Ugn and GC-C) and tight junction proteins (occludin, claudin-1 and ZO-1) was measured in the colon. Additionally, the level of pro-inflammatory cytokines (IL-8 and TNF-α) in serum was measured. After injecting the UC mice with the Gn overexpression vector, the body weight increased, and the frequency of loose stools and bloody stools was decreased. Intestinal permeability and histopathologic score were significantly reduced (P<0.05). The expression level of GC-C, Gn, Ugn, claudin-1 and ZO-1 was significantly increased (P<0.05). The level of IL-8 and TNF-α in the serum was significantly decreased (P<0.01). Therefore, the application of Gn overexpression vector can ameliorate the intestinal inflammatory injury and repair the mucosal barrier in colitis mice, which further suggests the clinical therapeutic potential of GC-C agonists in IBD.

scholarly journals THE COMPARATIVE ANALYSIS OF BIOMARKERS OF THE INFLAMMATION AT PATIENTS WITH PROGRESSING ODONTOGENNY PHLEGMONS AT TREATMENT BY SELECTIVE DEKONTAMINATIONS METHOD OF INTESTINES

2014 ◽  
Vol 10 (1) ◽  
pp. 43-45
Author(s):  
А. Зыкин ◽  
A. Zykin ◽  
А. Громов ◽  
A. Gromov
Keyword(s):  
Comparative Analysis ◽  
Bacterial Translocation ◽  
Inflammatory Mediators ◽  
Selective Decontamination ◽  
Diagnosis And Treatment ◽  
Control Group ◽  
Tnf Α ◽  
And Control

<p>This work was devoted to improve the efficiency of diagnosis and treatment of patients with odontogenic phlegmons based on studying the role of inflammatory mediators in the pathogenesis of progressive odon- togenic infection . For this purpose, we compare the level of IL-4, 6, TNF-α in the study and control group, which revealed a hairdryer bacterial translocation and its impact on the pathogenesis of odontogenic sepsis. Investigation of the influence of the method of selec- tive decontamination of the level of pro-inflammatory interleukins (TNF-α, IL-6) and anti- IL-4 was determined multidirected chosen method of treatment. Levels of IL-6, TNF and decrease no change in anti- IL-4 suggests a method of selective decontamination of inefficiency within four days .</p><p> </p>

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