Measurement of tear production and establishment of reference values in guinea pigs (Cavia porcellus) using a modified Schirmer tear test

2019 ◽  
Vol 186 (10) ◽  
pp. 321-321
Author(s):  
Anna Linda Nogradi ◽  
Zsolt Szentgáli ◽  
Márton Battay ◽  
Iain Cope ◽  
János Gál ◽  
...  
Keyword(s):  
Reference Values ◽  
Guinea Pigs ◽  
Small Volume ◽  
Clinical Signs ◽  
Random Order ◽  
Cavia Porcellus ◽  
Tear Production ◽  
Schirmer Tear Test ◽  
The Mean ◽  
One Year

BackgroundPast studies have shown that 5-mm Schirmer tear tests (STTs) are difficult to interpret in guinea pigs. Modified 2.5-mm STT strips are more adequate for measuring the small volume of tear production in these animals. The aim of this study was to establish reference values for the modified 2.5-mm STT strips.MethodsModified STT measurements were performed in random order in both eyes of 50 guinea pig patients. The wetting of the strip was noted at 30 seconds and at 1 minute.ResultsThe mean±sd STT values for both eyes were 7.58±3.19 mm at 30 seconds and 10.42±3.92 mm at 60 seconds. The median was 8 mm at 30 seconds and 10 mm at 60 seconds for both eyes. Animals under one year of age had lower STT values. STT values at 30 and 60 seconds can both be used. Both eyes should be measured when possible.ConclusionThe authors recommend values above 8 mm at 30 seconds and 10 mm at 60 seconds to be considered normal when using a 2.5-mm STT strip. There are ambiguous areas at 5–8 mm and 6–10 mm. If in these ranges the results should be combined with clinical signs and judgement.

scholarly journals Evaluation of tear production with modified Schirmer tear test-1 during the neonatal period in cats

2011 ◽  
Vol 31 (4) ◽  
pp. 350-354 ◽  
Author(s):  
L.F. Conceição ◽  
A.P. Ribeiro ◽  
M.L.M. Torres ◽  
M.L.G. Lourenço ◽  
J.L. Laus
Keyword(s):  
Reference Values ◽  
Neonatal Period ◽  
Positive Correlation ◽  
Tear Secretion ◽  
Tear Production ◽  
Schirmer Tear Test ◽  
Maturity Parameters ◽  
Time Point

The study aimed to evaluate tear production by means of modified Schirmer tear tes-1 (mSTT-1) in neonate cats. Likewise, correlation between mSTT-1 and STT-1 was assessed in vitro. Standard SST strips were cut in half and after eye lid opening, tear production of neonates (n=15) was daily measured in both eyes (mSTT-1), until the 7th day, and at day 14, 21, and 28. Animals were daily weighted until 28 days of age. Results were compared statistically (P<0.05). During the first 7 days, the overall mSTT-1 mean was 0.76 wetting/minute. Significant differences between right and left eyes were not observed at any time point (P=1.00). Tear secretion increased significantly, from the 14th to 28th day, in comparison with 7 first days (P<0.05). Positive correlation between maturity parameters and tear secretion was observed (P<0.0001). Distance between slopes of each strip changed significantly (P<0.0001). It was concluded that tear secretion in the neonatal period of cats is very below the reference values described for young and adults of the same species. It is not possible to extrapolate results obtained with mSTT-1 to standard STT-1.

scholarly journals Electrolyte changes in the neonates receiving phototherapy

2020 ◽  
Vol 7 (8) ◽  
pp. 1753
Author(s):  
Abhishek Purohit ◽  
Suresh Kumar Verma
Keyword(s):  
Neonatal Jaundice ◽  
Serum Bilirubin ◽  
Neonatal Period ◽  
Clinical Signs ◽  
Electrolyte Imbalance ◽  
Sodium Potassium ◽  
Before And After ◽  
Preterm Babies ◽  
The Mean ◽  
One Year

Background: Neonatal jaundice is the most commonly observed clinical condition of early neonatal period. It remains a common cause of readmission and important cause of concern for the parents and clinicians. Therefore, appropriate management of neonatal hyperbilirubinemia is of paramount importance. Phototherapy plays a significant role in its management but this modality is not devoid of complications. Authors studied electrolyte changes in the neonates, who received phototherapy.Methods: This perspective hospital based observational study conducted over the period of one year on 90 eligible admitted neonates who received phototherapy as per AAP guideline. Serum bilirubin, sodium, potassium and calcium were determined before and after termination of phototherapy. The first samples were considered as controls and compared with the samples collected after termination of phototherapy.Results: The incidence of LBW babies in this study was 21.9%, while mean birth weight and gestational age was2.76±0.38 kg and 38.34±0.88 weeks respectively. Mean duration of phototherapy was 38.48±09.34 hours. The mean sodium, potassium and calcium level before therapy were 146.6±6.2 mg/dl, 4.7±0.47 mg/dl and 9.4±0.73 mg/dl respectively. Authors found statistically significant changes in the electrolyte’s levels (Na+ 141.3±6.1, K+ 4.2±0.51 and Ca+2 8.4±0.68 respectively), after phototherapy however none of any neonate shown any clinical signs of dyselectolytemia.Conclusions: Neonates undergoing phototherapy are at greater risk of dyselectrolytemia especially in preterm babies therefore close clinical and biochemical monitoring is required for the prompt management of any significant electrolyte imbalance.

scholarly journals Typhlitis Caused by IntestinalSerpulina-Like Bacteria in Domestic Guinea Pigs (Cavia porcellus)

1998 ◽  
Vol 36 (3) ◽  
pp. 690-694 ◽  
Author(s):  
Mia Vanrobaeys ◽  
Peter De Herdt ◽  
Richard Ducatelle ◽  
Luc A. Devriese ◽  
Gerard Charlier ◽  
...  
Keyword(s):  
Guinea Pigs ◽  
Intestinal Tract ◽  
Fatal Outcome ◽  
Clinical Signs ◽  
Disease Spread ◽  
Cavia Porcellus ◽  
Serpulina Hyodysenteriae ◽  
Large Numbers ◽  
History Of ◽  
The University

Between January 1992 and December 1996, Serpulina-like bacteria were demonstrated in intestinal tract lesions from 37 of 88 guinea pigs submitted to the University of Ghent in Ghent, Belgium, for necropsy because of disease and death from different unknown causes. All infected animals had a history of sudden death with minimal introductory clinical signs. Occasionally, they produced yellow, slimy feces or showed nervous signs, but the condition always had a fatal outcome within 24 h. When larger colonies of guinea pigs were involved, the disease spread very rapidly unless treatment with ronidazole was initiated. Lesions consisted of a catarrhal or hemorrhagic inflammation of the colon and cecum (typhlitis). Electron microscopy demonstrated the presence of large numbers ofSerpulina-like organisms adhering to the cecal mucosae of these animals. Attempts to isolate the agents failed. The organisms did not stain by an immunofluorescence technique for the detection ofSerpulina hyodysenteriae. The present data provide evidence that intestinal Serpulina-like organisms can be important as a cause of disease in guinea pigs.

scholarly journals Effect of methadone and acepromazine premedication on tear production in dogs

2018 ◽  
Vol 5 (1) ◽  
pp. e000298 ◽  
Author(s):  
Hayley A Volk ◽  
Ellie West ◽  
Rose Non Linn-Pearl ◽  
Georgina V Fricker ◽  
Ambra Panti ◽  
...  
Keyword(s):  
General Anaesthesia ◽  
General Linear Model ◽  
Corneal Ulceration ◽  
Tear Production ◽  
Lower Baseline ◽  
Schirmer Tear Test ◽  
Left And Right ◽  
The Mean ◽  
The Uk ◽  
Referral Practice

ObjectivesTo evaluate the combined effect of intramuscular acepromazine and methadone on tear production in dogs undergoing general anaesthesia for elective, non-ocular procedures.DesignProspective, non-randomised, pre-post treatment study.SettingPatients were recruited from a referral practice in the UK.MethodsThirty client-owned dogs were enrolled in this study and received a combined intramuscular premedication of methadone (0.3 mg/kg) and acepromazine (0.02 mg/kg) before general anaesthesia for elective, non-ocular procedures. Full ophthalmic examination was performed and tear production was quantified using the Schirmer tear test-1 (STT-1). On the day of general anaesthesia, an STT-1 was performed before (STT-1a) and after (STT-1b) intramuscular premedication with methadone/acepromazine.ResultsUsing a general linear model, a significant effect on STT-1 results was found for premedication with methadone/acepromazine (P=0.013), but not eye laterality (P=0.527). Following premedication, there was a significant reduction observed in the mean STT-1 readings of left and right eyes between STT-1a (20.4±2.8 mm/min) and STT-1b (16.9±4.1 mm/min; P<0.001). Significantly more dogs had an STT-1 reading less than 15 mm/min in one or both eyes after premedication (30 per cent; 9/30 dogs) compared with before premedication (6.7 per cent; 2/30 dogs; P=0.042).ConclusionsAn intramuscular premedication of methadone and acepromazine results in a decrease in tear production in dogs before elective general anaesthesia. This may contribute to the risk of ocular morbidities, such as corneal ulceration, particularly in patients with lower baseline tear production.

scholarly journals Reference Values for the Ophthalmic Schirmer Tear Test and the Intraocular Pressure in Healthy Chinchillas

2016 ◽  
Vol 60 (3) ◽  
pp. 29-33
Author(s):  
M. Richards ◽  
A. Trbolová
Keyword(s):  
Intraocular Pressure ◽  
Reference Range ◽  
The Novel ◽  
Topical Anaesthesia ◽  
Tear Production ◽  
Schirmer Tear Test ◽  
Significant Difference ◽  
Before And After ◽  
Left And Right ◽  
The Mean

Abstract The objective of this study was to measure the intraocular pressure (IOP) and tear production before and after topical anaesthesia in healthy chinchillas (Chinchilla lanigera). Thirteen healthy non-sedated chinchillas (eight males and five females) were used in this study. The tear production was measured by the novel endodontic paper point tear test (PPTT) using Roeko Colour No. 30 Paper points. Following the PPTT, one drop of 0.4 % oxybuprokainium chloride was added to the eye to anaesthetise the cornea and the IOP was measured using the Tono-Pen Avia®Vet. Excess anaesthetic was removed from the conjunctival fornix using a sterile cotton tipped applicator and the PPTT II was performed. The PPTT I and II were measured in 26 eyes, mean ± standard deviations (SD) were 7.98 ± 1.95 mm.min−1, and 9.71 ± 3.52 mm.min−1 respectively. The IOP was measured in 20 eyes, and the mean ± SD was 28.52 ± 12.48 mmHg (35.50 ± 9.31 mmHg in males and 21.53 ± 11.57 mmHg in females). There was no significant difference in the PPTT results between the left and right eyes or between the male and female groups. The males were found to have a significantly higher IOP than females and the PPTT II was significantly greater than the PPTT I. The PPTT test proved to be effective, easy to use, and reliable, causing little apparent discomfort to the chinchillas and could prove to be a much more effective tool than the Schirmer tear test for the evaluation of the tear production in animals with small eyes and/or low aqueous tear production. The mean intraocular pressure proved to be much higher in this population of chinchillas than those previously studied and so further investigation is warranted before a reliable reference range may be produced.

scholarly journals Ultrasonographic deteCTION of abdominal abScess in two guinea pigs

2000 ◽  
Vol 48 (3) ◽  
pp. 271-276 ◽  
Author(s):  
Suzanne Zorn ◽  
V. Molnár ◽  
F. Bíró ◽  
A. Beregi
Keyword(s):  
Weight Loss ◽  
Guinea Pigs ◽  
Ultrasound Examination ◽  
Histopathological Examination ◽  
Clinical Signs ◽  
The Other ◽  
Thick Wall ◽  
Cavia Porcellus ◽  
Abdominal Abscess ◽  
Pelvic Region

Two guinea pigs (Cavia porcellus) with clinical signs of anorexia, weight loss, depression and abdominal enlargement were examined. During ultrasound examination, a fluid-filled anechogenic structure 3–4 cm in diameter, with echogenic spots and a highly echogenic thick wall, was found in the pelvic region in one case and connected to the liver in the other case. An abscess or a cyst was suspected and surgical treatment including laparotomy was performed. By histopathological examination performed after surgery, a liver abscess was diagnosed in one guinea pig and an abscess in the pelvic region in the other animal.

Effects of two concentrations of topical tropicamide on the Schirmer tear test in clinically normal cats

2016 ◽  
Vol 18 (12) ◽  
pp. 965-969 ◽  
Author(s):  
Masoud Selk Ghaffari ◽  
Reza Javadzadeh ◽  
Seyed Mehdi Rajaei
Keyword(s):  
Distilled Water ◽  
Clinically Normal ◽  
Tear Production ◽  
Schirmer Tear Test ◽  
Production Measurement ◽  
The Mean ◽  
Ophthalmic Solutions

Objectives The aim of this study was to evaluate the effect of topical tropicamide at two concentrations (0.5% and 1.0%) on the Schirmer tear test (STT) results in clinically normal cats. Methods Twenty-four adult domestic shorthair cats were randomly assigned to three groups. In all three groups, ophthalmic solutions were instilled in a randomly selected eye and the opposite eye served as the control. In groups 1, 2 and 3 one drop of 0.5% tropicamide, 1.0% tropicamide and distilled water was used, respectively. Tear production in both eyes was tested 30 and 60 mins after instillation in all three groups. Results Baseline mean ± SEM STT values for the treated eyes in groups 1, 2 and 3 were 13.37 ± 2.91 mm/min, 10.87 ± 1.39 mm/min and 11.37 ± 1.65 mm/min, respectively. Thirty minutes after the drug instillation in the treated eye, mean ± SEM STT values in groups 1, 2 and 3 were 4.87 ± 3.05 mm/min, 2.00 ± 0.84 mm/min and 11.25 ± 1.81 mm/min, respectively. The mean ± SEM STT levels of the treated eye after 60 mins were 3.75 ± 1.87 mm/min, 0.5 ± 0.37 mm/min and 11.42 ± 1.78 mm/min in groups 1, 2 and 3, respectively. Conclusions and relevance Use of 0.5% topical tropicamide, instead of 1.0% tropicamide, as a mydriatic agent, can be recommended in cats. Tear production measurement should be performed prior to the administration of tropicamide, regardless of the concentration of this drug.

scholarly journals Effects of avocado seed extracts on growth performance and caecal flora of guinea pig (Cavia porcellus)

2021 ◽  
Vol 14 (9) ◽  
pp. 3241-3253
Author(s):  
Alain Serge Tatsinkou ◽  
Emile Miegoue ◽  
Marie Noëlle Bertine Noumbissi ◽  
Camara Sawa ◽  
Hervé Kuitche Mube ◽  
...  
Keyword(s):  
Developing Countries ◽  
Guinea Pig ◽  
Growth Performance ◽  
Guinea Pigs ◽  
Cavia Porcellus ◽  
E Coli ◽  
Protein Sources ◽  
The Mean ◽  
Avocado Seed ◽  
Seed Extracts

The diversification of animal protein sources is a beneficial strategy to fill the protein deficit that all developing countries like Cameroon are facing. This work was initiated with the aim of increasing the productivity of guinea pigs through the use of avocado seed extracts. Four experimental rations (T0, T1, T2 and T3) were formulated with graded level of avocado seed extracts (0; 100 g; 200 g and 300 g of extracts per 100 kg of food respectively). Each treatment was tested on 15 females and 3 males divided into 3 subgroups. The results reveal that the mean weights of the animals were significantly the highest: at farrowing (87.00 g); at weaning (160.80 g) and at 8 weeks of age (234.40 g) were obtained with the T1 treatment. The significantly highest total gains (147.4 g) were obtained with the T1 treatment, the T3 treatment having given the lowest mean gain. In animals receiving T0 and T1 treatments, the level of lactobacilli was higher than the level of E. coli. The reverse was observed with T2 and T3 treatments. The use of avocado seed extracts can be considered at an incorporation rate of 100 g / 100 kg in guinea pigs feed. La diversification des sources de protéines animales est une stratégie salutaire pour combler le déficit protéique dont font face tous les pays en développement à l’instar du Cameroun. Ce travail a été initié dans l’optique d’accroître la productivité des cobayes à travers l’utilisation des extraits de noyaux d’avocat (ENA). Quatre rations expérimentales (T0, T1, T2 et T3) ont été formulées contenant respectivement 0 ; 100 g ; 200 g et 300 g de ENA pour 100 kg d’aliment. Chacune des rations a été testées sur 15 femelles et 3 mâles divisés en 3 sous-lots de 6 sujets chacun. Les résultats ont révélé que les poids moyens des animaux significativement les plus élevés : à la mise bas (87,00 g) ; au sevrage (160,80 g) et à 8 semaines d’âge (234,40 g) ont été obtenus avec les animaux recevant 100 g/100 kg d’extrait de noyau d’avocat (T1). Les gains totaux significativement les plus élevés (147,4 g) ont été obtenus avec le traitement T1, le traitement T3 ayant donné le gain moyen le plus bas. Chez les animaux recevant les traitements T0 et T1, le taux de lactobacilles a été supérieur au taux de colibacilles. L’inverse a été observé avec les traitements T2 et T3. L’utilisation des extraits des noyaux d’avocat peut être envisagée à un taux d’incorporation de 100g/100kg d’aliment chez le cobaye.

Sonographically guided, transcatheter foam sclerotherapy of the great saphenous vein

Phlebologie ◽  
2007 ◽  
Vol 36 (06) ◽  
pp. 309-312 ◽  
Author(s):  
T. Schulz ◽  
M. Jünger ◽  
M. Hahn
Keyword(s):  
Saphenous Vein ◽  
Great Saphenous Vein ◽  
Clinical Stage ◽  
Clinical Signs ◽  
Cost Effective ◽  
Duplex Sonography ◽  
Foam Sclerotherapy ◽  
Average Loss ◽  
One Year ◽  
The Cost

Summary Objective: The goal of the study was to assess the effectiveness and patient tolerability of single-session, sonographically guided, transcatheter foam sclerotherapy and to evaluate its economic impact. Patients, methods: We treated 20 patients with a total of 22 varicoses of the great saphenous vein (GSV) in Hach stage III-IV, clinical stage C2-C5 and a mean GSV diameter of 9 mm (range: 7 to 13 mm). We used 10 ml 3% Aethoxysklerol®. Additional varicoses of the auxiliary veins of the GSV were sclerosed immediately afterwards. Results: The occlusion rate in the treated GSVs was 100% one week after therapy as demonstrated with duplex sonography. The cost of the procedure was 207.91 E including follow-up visit, with an average loss of working time of 0.6 days. After one year one patient showed clinical signs of recurrent varicosis in the GSV; duplex sonography showed reflux in the region of the saphenofemoral junction in a total of seven patients (32% of the treated GSVs). Conclusion: Transcatheter foam sclerotherapy of the GSV is a cost-effective, safe method of treating varicoses of GSV and broadens the spectrum of therapeutic options. Relapses can be re-treated inexpensively with sclerotherapy.

Alterations in Ruminal Fluid and Serum Biochemical Constituents in Goats Affected with Ruminal Acidosis

2018 ◽  
Vol 13 (03) ◽  
Author(s):  
P. R. Chavelikar ◽  
G. and Neha Rao C. Mandali ◽  
Neha Rao
Keyword(s):  
Rumen Fluid ◽  
Clinical Signs ◽  
Small Ruminants ◽  
Ruminal Fluid ◽  
Mean Values ◽  
Biochemical Constituents ◽  
Ruminal Acidosis ◽  
Methylene Blue Reduction ◽  
Serum Biochemical ◽  
The Mean

Ruminal acidosis is an important clinical emergency in small ruminants. In this study, eight healthy farm goats and 24 goats presented at TVCC of the college of Veterinary Sciences and A.H., Anand with clinical signs of ruminal acidosis and having rumen liquor pH below 6 were examined for alterations in the ruminal fluid and serum biochemical parameters. Among various rumen fluid parameters evaluated, the mean values of rumen fluid pH decreased significantly (4.71±0.11 vs. 6.90±0.10), while sediment activity time (46.67±1.20 vs. 24.50±0.78 min) and methylene blue reduction time (29.50±0.73 vs. 10.03±0.27 min) increased significantly in acidotic goats. The normal greenish, aromatic viscous color, odour and consistency of rumen fluid of healthy goats also changed to milky grey/creamy, sour/pungent watery in acidotic goats. The rumen protozoal activity decreased to nil in acidotic goats as compared to the healthy goats. Among various serum biochemical constituents, the mean values of glucose (92.43±1.37 vs. 74.13±1.83 mg/dl), BUN (26.49±0.47 vs. 22.63±1.19 mg/dl), serum creatinine (01.01±0.02 vs. 00.83±0.02 mg/dl) and albumin (03.22±0.03 vs. 03.05±0.05 g/dl), ALT (56.75±1.55 vs. 27.88±1.14 IU/L) and AST (93.25±1.82 vs. 54.00±1.75 IU/L), increased significantly, while there was significant decrease in serum calcium (09.09±0.14 vs. 10.29±0.08 mg/dl) in acidotic goats. The mean values of alkaline phosphatase (IU/L) in acidotic goats increased non-significantly from the base values of healthy goats.

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