scholarly journals Evaluation of tear production with modified Schirmer tear test-1 during the neonatal period in cats

2011 ◽  
Vol 31 (4) ◽  
pp. 350-354 ◽  
Author(s):  
L.F. Conceição ◽  
A.P. Ribeiro ◽  
M.L.M. Torres ◽  
M.L.G. Lourenço ◽  
J.L. Laus
Keyword(s):  
Reference Values ◽  
Neonatal Period ◽  
Positive Correlation ◽  
Tear Secretion ◽  
Tear Production ◽  
Schirmer Tear Test ◽  
Maturity Parameters ◽  
Time Point

The study aimed to evaluate tear production by means of modified Schirmer tear tes-1 (mSTT-1) in neonate cats. Likewise, correlation between mSTT-1 and STT-1 was assessed in vitro. Standard SST strips were cut in half and after eye lid opening, tear production of neonates (n=15) was daily measured in both eyes (mSTT-1), until the 7th day, and at day 14, 21, and 28. Animals were daily weighted until 28 days of age. Results were compared statistically (P<0.05). During the first 7 days, the overall mSTT-1 mean was 0.76 wetting/minute. Significant differences between right and left eyes were not observed at any time point (P=1.00). Tear secretion increased significantly, from the 14th to 28th day, in comparison with 7 first days (P<0.05). Positive correlation between maturity parameters and tear secretion was observed (P<0.0001). Distance between slopes of each strip changed significantly (P<0.0001). It was concluded that tear secretion in the neonatal period of cats is very below the reference values described for young and adults of the same species. It is not possible to extrapolate results obtained with mSTT-1 to standard STT-1.

Measurement of tear production and establishment of reference values in guinea pigs (Cavia porcellus) using a modified Schirmer tear test

2019 ◽  
Vol 186 (10) ◽  
pp. 321-321
Author(s):  
Anna Linda Nogradi ◽  
Zsolt Szentgáli ◽  
Márton Battay ◽  
Iain Cope ◽  
János Gál ◽  
...  
Keyword(s):  
Reference Values ◽  
Guinea Pigs ◽  
Small Volume ◽  
Clinical Signs ◽  
Random Order ◽  
Cavia Porcellus ◽  
Tear Production ◽  
Schirmer Tear Test ◽  
The Mean ◽  
One Year

BackgroundPast studies have shown that 5-mm Schirmer tear tests (STTs) are difficult to interpret in guinea pigs. Modified 2.5-mm STT strips are more adequate for measuring the small volume of tear production in these animals. The aim of this study was to establish reference values for the modified 2.5-mm STT strips.MethodsModified STT measurements were performed in random order in both eyes of 50 guinea pig patients. The wetting of the strip was noted at 30 seconds and at 1 minute.ResultsThe mean±sd STT values for both eyes were 7.58±3.19 mm at 30 seconds and 10.42±3.92 mm at 60 seconds. The median was 8 mm at 30 seconds and 10 mm at 60 seconds for both eyes. Animals under one year of age had lower STT values. STT values at 30 and 60 seconds can both be used. Both eyes should be measured when possible.ConclusionThe authors recommend values above 8 mm at 30 seconds and 10 mm at 60 seconds to be considered normal when using a 2.5-mm STT strip. There are ambiguous areas at 5–8 mm and 6–10 mm. If in these ranges the results should be combined with clinical signs and judgement.

scholarly journals Reference values of Schirmer tear test in sheep and the effect of season on the test results

2019 ◽  
Vol 67 (4) ◽  
pp. 553-560
Author(s):  
Anna Dedousi ◽  
Maria A. Karatzia ◽  
Panagiotis D. Katsoulos
Keyword(s):  
Reference Values ◽  
Reference Range ◽  
Tear Film ◽  
Standing Position ◽  
Potential Effect ◽  
Test Results ◽  
Tear Secretion ◽  
Mixed Breed ◽  
Schirmer Tear Test

The aim of this study was to determine the reference range of Schirmer tear test (STT) values in sheep using Greek indigenous and mixed breeds and to investigate the potential effect of breed and season on these values. Ninety (30 Chios, 30 Florina and 30 Lacaune mixed breed) ewes aged 2 years or more were used for the study. The aqueous portion of the tear film was measured using Schirmer tear test (STT) commercial strips bilaterally by the same investigator with the animal in standing position. The average STT value recorded was 18.45 ± 3.93 mm/min and the range 10.8–26.2 mm/min. STT was significantly affected by the season and the recorded values were significantly higher in summer compared to winter. The breed had no significant effect on tear secretion. The results of the present study provide a reference range of STT values in sheep and indicate that tear secretion is significantly affected by the season.

The Strong Positive Correlation between Factor VII Clotting Activity Using Bovine Thromboplastin and the Activated Factor VII Level

1995 ◽  
Vol 73 (03) ◽  
pp. 429-434 ◽  
Author(s):  
Kazuomi Kario ◽  
Takefumi Matsuo ◽  
Reiko Asada ◽  
Toshiyuki Sakata ◽  
Hisao Kato ◽  
...  
Keyword(s):  
Risk Factor ◽  
Bovine Brain ◽  
Factor Vii ◽  
Rabbit Brain ◽  
Strong Positive Correlation ◽  
Chromogenic Substrate ◽  
Moderate Correlation ◽  
Positive Correlation ◽  
Activated Factor Vii

SummaryWe compared factor VII clotting activity (FVIIc) assays using different thromboplastins to determine which is the most sensitive for activated FVII (FVIIa) or for FVII antigen (FVIIag). FVIIc levels were measured using thromboplastins derived from bovine brain (FVIIc Bov), human placenta (FVIIc Hum), and rabbit brain (FVIIc Rab). FVIIa levels were measured by fluorogenic assays using human soluble tissue factor (rsTF) or bovine rsTF. We also measured FVII activity by an amidolytic assay (FVIIc:am Hum) using human thromboplastin and a chromogenic substrate for thrombin. FVIIag levels were determined by ELISA. In the FVIIa assay, the reaction time obtained from using bovine rsTF was shorter than that with human rsTF, suggesting that the interaction of plasma FVIIa with bovine rsTF was stronger than with human rsTF. The plasma FVIIa levels measured using human rsTF and bovine rsTF were almost the same (r=0.947, p<0.0001). Among the three FVIIc assays, FVIIc Bov had the strongest positive correlation with the plasma FVIIa level (r=0.886, p<0.000l), but had no correlation with FVIIag. An increase of 1 ng/ml in the plasma FVIIa level yielded a 27.9% increase of FVIIc Bov. Plasma FVIIc Hum and FVIIc:am Hum showed moderate correlations with both FVIIa (r=0.520, p<0.02 and r=0.569, p<0.01, respectively) and FVIIag (r=0.438, p<0.05 and r=0.468, p<0.05, respectively). FVIIc Rab had the lowest correlation with FVIIa (r=0.367, p<0.1), but had a moderate correlation with FVIIag (r=0.436, p<0.05). After in vitro cold activation, FVIIc Bov levels increased the most and FVIIc:am levels showed the least change. These findings indicate that consideration of the thromboplastin used for assay is necessary when assessing the clinical significance of FVII activity as a cardiovascular risk factor.

Effect of acepromazine or xylazine on tear production as measured by Schirmer tear test in normal cats

2010 ◽  
Vol 13 (1) ◽  
pp. 1-3 ◽  
Author(s):  
Masoud Selk Ghaffari ◽  
Abdolali Malmasi ◽  
Saied Bokaie
Keyword(s):  
Tear Production ◽  
Schirmer Tear Test

Reference values for intraocular pressure and Schirmer tear test in clinically normal Sanjabi sheep

2011 ◽  
Vol 97 (1-3) ◽  
pp. 101-103 ◽  
Author(s):  
Masoud Selk Ghaffari ◽  
Mohammad Shojaei ◽  
Amin Sabzevari ◽  
Nargess Khorami
Keyword(s):  
Intraocular Pressure ◽  
Reference Values ◽  
Clinically Normal ◽  
Schirmer Tear Test

Neonatal IL-4 exposure decreases adipogenesis of male rats into adulthood

2021 ◽  
Author(s):  
Tammy Ying ◽  
Thea N. Golden ◽  
Lan Cheng ◽  
Jeff Ishibashi ◽  
Patrick Seale ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Neonatal Period ◽  
Uncoupling Protein ◽  
Adipogenic Differentiation ◽  
Male Rats ◽  
Interleukin 4 ◽  
Sprague Dawley ◽  
Fat Cell ◽  
Subcutaneous White Adipose Tissue

The cytokine interleukin 4 (IL-4) can increase beige adipogenesis in adult rodents. However, neonatal animals use a distinct adipocyte precursor compartment for adipogenesis compared to adults. In this study, we address whether IL-4 can induce persistent effects on adipose tissue when administered subcutaneously in the interscapular region during the neonatal period in Sprague Dawley rats. We injected IL-4 into neonatal male rats during postnatal days 1-6, followed by analysis of adipose tissue and adipocyte precursors at 2 weeks and 10 weeks of age. Adipocyte precursors were cultured and subjected to differentiation in vitro. We found that a short and transient IL-4 exposure in neonates upregulated uncoupling protein 1 (Ucp1) mRNA expression and decreased fat cell size in subcutaneous white adipose tissue (WAT). Adipocyte precursors from mature rats that had been treated with IL-4 as neonates displayed a decrease in adiponectin (Adipoq) but no change in Ucp1 expression, as compared to controls. Thus, neonatal IL-4 induces acute beige adipogenesis and decreases adipogenic differentiation capacity long term. Overall, these findings indicate that the neonatal period is critical for adipocyte development and may influence the later onset of obesity.

Tear production in canine neonates - evaluation using a modified Schirmer tear test

2012 ◽  
Vol 16 (3) ◽  
pp. 175-179 ◽  
Author(s):  
Enry Garcia da Silva ◽  
Lynne S. Sandmeyer ◽  
Juliet R. Gionfriddo ◽  
Fabiano Montiani-Ferreira ◽  
Paula D. Galera
Keyword(s):  
Tear Production ◽  
Schirmer Tear Test

Simplified estimation of forage degradability in the rumen assuming zero-order degradation kinetics

2008 ◽  
Vol 147 (3) ◽  
pp. 225-240 ◽  
Author(s):  
M. S. DHANOA ◽  
S. LÓPEZ ◽  
R. SANDERSON ◽  
J. FRANCE
Keyword(s):  
Reference Values ◽  
Incubation Time ◽  
Lag Time ◽  
Zero Order ◽  
Regression Equations ◽  
Order Model ◽  
First Order ◽  
Simplified Procedure ◽  
The Impact ◽  
Time Point

SUMMARYIn the present paper, a simplified procedure using few in situ data points is derived and then evaluated (using a large database) against reference values estimated with the standard nylon bag first-order kinetics model. The procedure proposed involved a two-stage mathematical process, with a statistical prediction of some degradation parameters (such as lag time) and then a kinetic model derived by assuming degradation follows zero-order kinetics to determine effective degradability in the rumen (E). In addition to the estimation of washout fraction and discrete lag, which is common to both procedures, the simplified procedure requires measurement of dry matter losses at one incubation time point only. Thus, interference of the animal rumen will be much reduced, which will lead to increased capacity for feed evaluation. Calibration of the zero-order model against the first-order model showed that suitable estimates of E can be obtained with disappearance at 24, 48 or 72 h as the single incubation end time point. The strength of the calibration is such that an end incubation time point as low as 24 h may be sufficient, which may reduce substantially the total incubation time required and thus the impact on the experimental animal. Relevant regression equations to predict reference values of parameters such as lag time or E are also developed and validated.

scholarly journals Reference values for the six-minute pegboard and ring test in healthy adults in Brazil

2018 ◽  
Vol 44 (3) ◽  
pp. 190-194 ◽  
Author(s):  
Vanessa Pereira Lima ◽  
Fabiana Damasceno Almeida ◽  
Tania Janaudis-Ferreira ◽  
Bianca Carmona ◽  
Giane Amorim Ribeiro-Samora ◽  
...  
Keyword(s):  
Physical Activity ◽  
Data Analysis ◽  
Reference Values ◽  
Healthy Adults ◽  
Ring Test ◽  
Age Group ◽  
Upper Arm ◽  
Positive Correlation ◽  
The Difference ◽  
Test Result

ABSTRACT Objective: To determine reference values for the six-minute pegboard and ring test (6PBRT) in healthy adults in Brazil, correlating the results with arm length, circumference of the upper arm/forearm of the dominant arm, and the level of physical activity. Methods: The participants (all volunteers) performed two 6PBRTs, 30 min apart. They were instructed to move as many rings as possible in six minutes. The best test result was selected for data analysis. Results: The sample comprised 104 individuals, all over 30 years of age. Reference values were reported by age bracket. We found that age correlated with 6PBRT results. The number of rings moved was higher in the 30- to 39-year age group than in the > 80-year age group (430.25 ± 77.00 vs. 265.00 ± 65.75), and the difference was significant (p < 0.05). The 6PBRT results showed a weak, positive correlation with the level of physical activity (r = 0.358; p < 0.05) but did not correlate significantly with any other variable studied. Conclusions: In this study, we were able to determine reference values for the 6PBRT in healthy adults in Brazil. There was a correlation between 6PBRT results and age.

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