Populus trichocarpa MONOPTEROS/AUXIN RESPONSE FACTOR5 (ARF5) genes: comparative structure, sub-functionalization, and Populus–Arabidopsis microsyntenyThis article is one of a selection of papers published in the Special Issue on Poplar Research in Canada.

2007 ◽  
Vol 85 (11) ◽  
pp. 1058-1070 ◽  
Author(s):  
Lee A. Johnson ◽  
Carl J. Douglas
Keyword(s):  
Secondary Xylem ◽  
Populus Trichocarpa ◽  
Expression Patterns ◽  
Fold Increase ◽  
Duplication Event ◽  
Sequence Information ◽  
Auxin Response ◽  
Genome Duplication Event ◽  
Regulatory Module ◽  
Comparative Structure

The genome of Populus (poplar) has been shaped by a whole genome duplication event specific to the salicoid lineage. The MONOPTEROS (MP)/AUXIN RESPONSE FACTOR5 (ARF5) transcription factor plays a key role in auxin-mediated morphogenesis and vascular development in Arabidopsis , and may play a similar role in secondary xylem development in Populus. We used EST and genome sequence information to identify and characterize two duplicated Populus MP genes, PoptrMP1 and PoptrMP2. PoptrMP1 and PoptrMP2 DNA binding and other domains are highly conserved relative to Arabidopsis MP, while the glutamine-rich middle domains are divergent. The two PoptrMP genes are located on duplicated regions of linkage groups II and V. Comparative analysis of the surrounding genes in both the Populus and Arabidopsis genomes revealed a high degree of conservation of gene content and order extending over 11 genes in the immediate vicinity, but also specific changes to genomic regions surrounding each MP locus, providing insights into genome evolution. Expression studies showed that PoptrMP1 and PoptrMP2 have overlapping but distinct expression patterns, suggesting that subfunctionalization of the duplicated genes has occurred, with PoptrMP1 specialized for expression in developing secondary xylem. Transgenic Populus lines overexpressing PoptrMP1 exhibited a 2–4 fold increase in expression of a Populus AtHB8 homolog, a proposed MP target gene, confirming conservation of this regulatory module.

scholarly journals Consequences of Hox gene duplication in the vertebrates: an investigation of the zebrafish Hox paralogue group 1 genes

Development ◽  
2001 ◽  
Vol 128 (13) ◽  
pp. 2471-2484 ◽  
Author(s):  
James M. McClintock ◽  
Robin Carlson ◽  
Devon M. Mann ◽  
Victoria E. Prince
Keyword(s):  
Hox Genes ◽  
Genome Duplication ◽  
Expression Patterns ◽  
Detailed Comparison ◽  
Duplication Event ◽  
Amino Acid Sequences ◽  
Functional Domains ◽  
Genome Duplication Event ◽  
Basic Functions ◽  
Functional Capacities

As a result of a whole genome duplication event in the lineage leading to teleosts, the zebrafish has seven clusters of Hox patterning genes, rather than four, as described for tetrapod vertebrates. To investigate the consequences of this genome duplication, we have carried out a detailed comparison of genes from a single Hox paralogue group, paralogue group (PG) 1. We have analyzed the sequences, expression patterns and potential functions of all four of the zebrafish PG1 Hox genes, and compared our data with that available for the three mouse genes. As the basic functions of Hox genes appear to be tightly constrained, comparison with mouse data has allowed us to identify specific changes in the developmental roles of Hox genes that have occurred during vertebrate evolution. We have found variation in expression patterns, amino acid sequences within functional domains, and potential gene functions both within the PG1 genes of zebrafish, and in comparison to mouse PG1 genes. We observed novel expression patterns in the midbrain, such that zebrafish hoxa1a and hoxc1a are expressed anterior to the domain traditionally thought to be under Hox patterning control. The hoxc1a gene shows significant coding sequence changes in known functional domains, which correlate with a reduced capacity to cause posteriorizing transformations. Moreover, the hoxb1 duplicate genes have differing functional capacities, suggesting divergence after duplication. We also find that an intriguing function ‘shuffling’ between paralogues has occurred, such that one of the zebrafish hoxb1 duplicates, hoxb1b, performs the role in hindbrain patterning played in mouse by the non-orthologous Hoxa1 gene.

scholarly journals Populus trichocarpa (Black Cottonwood) As a Model for Plant Genomic Studies: A Review

2021 ◽  
pp. 270-275
Author(s):  
Navjit Kaur ◽  
Divya Dhawal Bhandari
Keyword(s):  
Populus Trichocarpa ◽  
Duplication Event ◽  
Plant Genomics ◽  
Black Cottonwood ◽  
Genome Duplication Event ◽  
Protein Coding ◽  
Model Plant ◽  
Genomics Research ◽  
Genomic Studies ◽  
Medicinal Properties

Numerous plants have been the subject of recent research in the pharmacological, cosmetic, and agro-alimentary domains due to their chemical composition and multiple therapeutic capabilities. Populus trichocarpa is one of the most common trees found in deciduous forests (Salicaceae family). The current study examines Populus trichocarpa as a model plant for plant genomics research, as well as the most recent findings on phytochemical composition and medicinal potential. More than 45,000 potential protein-coding genes were discovered. In the Populus genome, a whole-genome duplication event was discovered, with approximately 8,000 pairs of duplicated genes surviving. Furthermore, the reproductive biology of Populus provides new opportunities and challenges in the study and analysis of natural genetic and phenotypic variation. In the present review, we endeavour to describe and compile the available knowledge on Populus trichocarpa as a model plant for genomic investigations and to bring that material up to date of Populus trichocarpa's phytochemical and medicinal properties.

scholarly journals The ancient Salicoid genome duplication event: A platform for reconstruction of de Novo gene evolution in Populus trichocarpa

2021 ◽  
Author(s):  
Timothy B Yates ◽  
Kai Feng ◽  
Jin Zhang ◽  
Vasanth Singan ◽  
Sara S Jawdy ◽  
...  
Keyword(s):  
Genome Duplication ◽  
De Novo ◽  
Gene Evolution ◽  
Populus Trichocarpa ◽  
Duplication Event ◽  
Functional Enrichment ◽  
Orphan Genes ◽  
Genome Duplication Event ◽  
Orphan Gene ◽  
De Novo Gene

Abstract Orphan genes are characteristic genomic features that have no detectable homology to genes in any other species and represent an important attribute of genome evolution as sources of novel genetic functions. Here, we identified 445 genes specific to Populus trichocarpa. Of these, we performed deeper reconstruction of 13 orphan genes to provide evidence of de novo gene evolution. Populus and its sister genera Salix are particularly well suited for the study of orphan gene evolution because of the Salicoid whole-genome duplication event (WGD) which resulted in highly syntenic sister chromosomal segments across the Salicaceae. We leveraged this genomic feature to reconstruct de novo gene evolution from inter-genera, inter-species, and intra-genomic perspectives by comparing the syntenic regions within the P. trichocarpa reference, then P. deltoides, and finally Salix purpurea. Furthermore, we demonstrated that 86.5% of the putative orphan genes had evidence of transcription. Additionally, we also utilized the Populus genome-wide association mapping panel (GWAS), a collection of 1,084 undomesticated P. trichocarpa genotypes to further determine putative regulatory networks of orphan genes using expression quantitative trait loci (eQTL) mapping. Functional enrichment of these eQTL subnetworks identified common biological themes associated with orphan genes such as response to stress and defense response. We also identify a putative cis-element for a de novo gene and leverage conserved synteny to describe evolution of a putative transcription factor binding site. Overall, 45% of orphan genes were captured in trans-eQTL networks.

scholarly journals Global assessment of organ specific basal gene expression over a diurnal cycle with analyses of gene copies exhibiting cyclic expression patterns

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Yuan Lu ◽  
Mikki Boswell ◽  
William Boswell ◽  
Raquel Ybanez Salinas ◽  
Markita Savage ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Pattern ◽  
Genome Duplication ◽  
Functional Divergence ◽  
Expression Patterns ◽  
Duplication Event ◽  
Global Scale ◽  
Genome Duplication Event ◽  
Gene Copies ◽  
Cyclic Expression

Abstract Background Studying functional divergences between paralogs that originated from genome duplication is a significant topic in investigating molecular evolution. Genes that exhibit basal level cyclic expression patterns including circadian and light responsive genes are important physiological regulators. Temporal shifts in basal gene expression patterns are important factors to be considered when studying genetic functions. However, adequate efforts have not been applied to studying basal gene expression variation on a global scale to establish transcriptional activity baselines for each organ. Furthermore, the investigation of cyclic expression pattern comparisons between genome duplication created paralogs, and potential functional divergence between them has been neglected. To address these questions, we utilized a teleost fish species, Xiphophorus maculatus, and profiled gene expression within 9 organs at 3-h intervals throughout a 24-h diurnal period. Results Our results showed 1.3–21.9% of genes in different organs exhibited cyclic expression patterns, with eye showing the highest fraction of cycling genes while gonads yielded the lowest. A majority of the duplicated gene pairs exhibited divergences in their basal level expression patterns wherein only one paralog exhibited an oscillating expression pattern, or both paralogs exhibit oscillating expression patterns, but each gene duplicate showed a different peak expression time, and/or in different organs. Conclusions These observations suggest cyclic genes experienced significant sub-, neo-, or non-functionalization following the teleost genome duplication event. In addition, we developed a customized, web-accessible, gene expression browser to facilitate data mining and data visualization for the scientific community.

scholarly journals Slc4 Gene Family in Spotted Sea Bass (Lateolabrax maculatus): Structure, Evolution, and Expression Profiling in Response to Alkalinity Stress and Salinity Changes

Genes ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1271
Author(s):  
Ling-Yu Wang ◽  
Yuan Tian ◽  
Hai-Shen Wen ◽  
Peng Yu ◽  
Yang Liu ◽  
...  
Keyword(s):  
Gene Family ◽  
Expression Patterns ◽  
Purifying Selection ◽  
Sea Bass ◽  
Duplication Event ◽  
Structure Evolution ◽  
Genome Duplication Event ◽  
Physiological Processes ◽  
Lateolabrax Maculatus ◽  
Solute Carrier

The solute carrier 4 (SLC4) family is a class of cell membranes transporters involved in base transport that plays crucial roles in diverse physiological processes. In our study, 15 slc4 genes were identified and annotated in spotted sea bass, including five members of Cl−/HCO3− exchangers, eight genes coding Na+-dependent HCO3− transporters, and two copies of Na+-coupled borate transporters. The gene sequence and structure, chromosomal and syntenic arrangement, phylogenetic and evolution profiles were analyzed. Results showed that the slc4 gene in teleosts obviously expanded compared with higher vertebrates, arising from teleost-specific whole genome duplication event. Most gene sites of slc4 in spotted sea bass were under strong purifying selection during evolution, while positive selection sites were only detected in slc4a1b, slc4a8, and slc4a10b. Additionally, qRT-PCR results showed that different slc4 genes exhibited distinct branchial expression patterns after alkalinity and salinity stresses, of which the strongly responsive members may play essential roles during these physiological processes. Our study provides the systemic overview of the slc4 gene family in spotted sea bass and enables a better understanding for the evolution of this family and further deciphering the biological roles in maintaining ion and acid–base homeostasis in teleosts.

scholarly journals Fish Suppressors of Cytokine Signaling (SOCS): Gene Discovery, Modulation of Expression and Function

2011 ◽  
Vol 2011 ◽  
pp. 1-20 ◽  
Author(s):  
Tiehui Wang ◽  
Bartolomeo Gorgoglione ◽  
Tanja Maehr ◽  
Jason W. Holland ◽  
Jose L. González Vecino ◽  
...  
Keyword(s):  
Genome Duplication ◽  
Expression Patterns ◽  
Duplication Event ◽  
Poly I:C ◽  
Cytokine Signaling ◽  
Parasitic Infections ◽  
Genome Duplication Event ◽  
Specific Expression ◽  
Functional Studies ◽  
Suppressors Of Cytokine Signaling

The intracellular suppressors of cytokine signaling (SOCS) family members, including CISH and SOCS1 to 7 in mammals, are important regulators of cytokine signaling pathways. So far, the orthologues of all the eight mammalian SOCS members have been identified in fish, with several of them having multiple copies. Whilst fish CISH, SOCS3, and SOCS5 paralogues are possibly the result of the fish-specific whole genome duplication event, gene duplication or lineage-specific genome duplication may also contribute to some paralogues, as with the three trout SOCS2s and three zebrafish SOCS5s. Fish SOCS genes are broadly expressed and also show species-specific expression patterns. They can be upregulated by cytokines, such as IFN-γ, TNF-α, IL-1β, IL-6, and IL-21, by immune stimulants such as LPS, poly I:C, and PMA, as well as by viral, bacterial, and parasitic infections in member- and species-dependent manners. Initial functional studies demonstrate conserved mechanisms of fish SOCS action via JAK/STAT pathways.

scholarly journals Genome-Wide Identification and Functional Characterization of the PheE2F/DP Gene Family in Moso Bamboo

2020 ◽  
Author(s):  
Long Li ◽  
Qianqian Shi ◽  
Zhouqi Li ◽  
Jian Gao
Keyword(s):  
Cell Cycle ◽  
Regulatory Element ◽  
Cell Cycle Control ◽  
Expression Patterns ◽  
Functional Characterization ◽  
Divergence Time ◽  
Duplication Event ◽  
Moso Bamboo ◽  
Functional Verification ◽  
Genome Duplication Event

Abstract Background E2F/DP proteins have been shown to regulate genes implicated in cell cycle control and DNA repair. However, to date, research into the potential role of the Moso bamboo E2F/DP family has been limited.Results Here, we identified 23 E2F/DPs in the Moso bamboo genome, including nine E2F genes, six DP genes, eight DEL genes and one gene with a partial E2F domain. An estimation of the divergence time of the paralogous gene pairs suggested that the E2F/DP family expansion primarily occurred through a whole-genome duplication event. A regulatory element and coexpression network analysis indicated that E2F/DP regulated the expression of cell cycle-related genes. A yeast two-hybrid assay and expression analysis based on transcriptome data and in situ hybridization indicated that the PheE2F-PheDP complex played important roles in winter Moso bamboo shoot growth. The qRT-PCR results showed that the PheE2F/DPs exhibited diverse expression patterns in response to drought and salt treatment and diurnal cycles. Conclusion Our findings provide novel insights into the Moso bamboo E2F/DP family and partial experimental evidence for further functional verification of the PheE2F/DPs.

scholarly journals Genome-wide identification of candidate aquaporins involved in water accumulation of pomegranate outer seed coat

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11810
Author(s):  
Jianjian Liu ◽  
Gaihua Qin ◽  
Chunyan Liu ◽  
Xiuli Liu ◽  
Jie Zhou ◽  
...  
Keyword(s):  
Seed Coat ◽  
Expression Patterns ◽  
Purifying Selection ◽  
Transcript Abundance ◽  
Punica Granatum ◽  
Duplication Event ◽  
Genome Duplication Event ◽  
Specific Expression ◽  
Water Accumulation ◽  
Seed Coats

Aquaporins (AQPs) are a class of highly conserved integral membrane proteins that facilitate the uptake and transport of water and other small molecules across cell membranes. However, little is known about AQP genes in pomegranate (Punica granatum L.) and their potential role in water accumulation of the outer seed coat. We identified 38 PgrAQP genes in the pomegranate genome and divided them into five subfamilies based on a comparative analysis. Purifying selection played a role in the evolution of PgrAQP genes and a whole-genome duplication event in Myrtales may have contributed to the expansion of PgrTIP, PgrSIP, and PgrXIP genes. Transcriptome data analysis revealed that the PgrAQP genes exhibited different tissue-specific expression patterns. Among them, the transcript abundance of PgrPIPs were significantly higher than that of other subfamilies. The mRNA transcription levels of PgrPIP1.3, PgrPIP2.8, and PgrSIP1.2 showed a significant linear relationship with water accumulation in seed coats, indicating that PgrPIP1.3/PgrPIP2.8 located in the plasma membrane and PgrSIP1.2 proteins located on the tonoplast may be involved in water accumulation and contribute to the cell expansion of the outer seed coat, which then develops into juicy edible flesh. Overall, our results provided not only information on the characteristics and evolution of PgrAQPs, but also insights on the genetic improvement of outer seed coats.

scholarly journals Genome-wide survey of Gγ subunit gene family in eight Rosaceae and expression analysis of PbrGGs in pear (Pyrus bretschneideri)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Guodong Chen ◽  
Yang Li ◽  
Xin Qiao ◽  
Weike Duan ◽  
Cong Jin ◽  
...  
Keyword(s):  
Gene Family ◽  
Cellular Response ◽  
Expression Patterns ◽  
Purifying Selection ◽  
Duplication Event ◽  
Subunit Gene ◽  
Genome Duplication Event ◽  
Protein Motifs ◽  
Chinese White ◽  
Family Based

Abstract Background Heterotrimeric G-proteins, composed of Gα, Gβ and Gγ subunits, are important signal transmitters, mediating the cellular response to multiple stimuli in animals and plants. The Gγ subunit is an essential component of the G-protein, providing appropriate functional specificity to the heterotrimer complex and has been well studied in many species. However, the evolutionary history, expression pattern and functional characteristics of Gγ subunits has not been explored in the Rosaceae, representing many important fruit crops. Results In this study, 35 Gγ subunit genes were identified from the eight species belonging to the Rosaceae family. Based on the structural gene characteristics, conserved protein motifs and phylogenetic analysis of the Gγ subunit genes, the genes were classified into three clades. Purifying selection was shown to play an important role in the evolution of Gγ subunit genes, while a recent whole-genome duplication event was the principal force determining the expansion of the Gγ subunit gene family in the subfamily Maloideae. Gγ subunit genes exhibited diverse spatiotemporal expression patterns in Chinese white pear, including fruit, root, ovary and bud, and under abiotic stress conditions, the relative expression of Gγ subunit genes were up-regulated or down-regulated. In addition, seven of the Gγ subunit proteins in pear were located on the plasma membrane, in the cytoplasm or nucleus. Conclusion Overall, this study of the Gγ subunit gene family in eight Rosaceae species provided useful information to better understand the evolution and expression of these genes and facilitated further exploration of their functions in these important crop plants.

scholarly journals Genome-Wide Identification and Functional Characterization of the PheE2F/DP Gene Family in Moso Bamboo

2020 ◽  
Author(s):  
Long Li ◽  
Qianqian Shi ◽  
Jian Gao
Keyword(s):  
Cell Cycle ◽  
Regulatory Element ◽  
Cell Cycle Control ◽  
Expression Patterns ◽  
Functional Characterization ◽  
Divergence Time ◽  
Duplication Event ◽  
Moso Bamboo ◽  
Functional Verification ◽  
Genome Duplication Event

Abstract BackgroundE2F/DP proteins have been shown to regulate genes implicated in cell cycle control and DNA repair. However, to date, research into the potential role of Moso bamboo E2F/DP family has been limited.ResultsHere, we identified 24 E2F/DPs in the Moso bamboo genome including nine E2F, six DP, eight DEL and one gene with partial E2F domain. Estimation of the divergence time of paralogous gene pairs suggested that E2F/DP family expansion was mainly contributed by whole-genome duplication event. The regulatory element and coexpression network analysis indicated that E2F/DP regulated the expression of cell cycle-related genes. Yeast two hybrid assay and expression analysis based on transcriptome data and in situ hybridization indicated that PheE2F-PheDP complex played important roles in winter moso bamboo shoot growth. qRT-PCR results showed that PheE2F/DPs performed diverse expression patterns in response to drought and salt treatment and diurnal cycles. ConclusionOur findings provide novel insights into the Moso bamboo E2F/DP family and partial experimental evidence for further functional verification of the PheE2F/DPs.

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