Fungal succession on aspen poplar leaf litter

1975 ◽  
Vol 53 (16) ◽  
pp. 1640-1651 ◽  
Author(s):  
Suzanne Visser ◽  
D. Parkinson
Keyword(s):  
Soil Washing ◽  
Dry Weight ◽  
Fungal Mycelium ◽  
Trichoderma Spp ◽  
Fungal Succession ◽  
Agar Film ◽  
Volutella Ciliata ◽  
Aspen Tree ◽  
The Difference ◽  
Sampling Times

Fungi from the litter, fermentation, and humus (LFH) layers of a mature aspen tree stand were qualitatively assessed using the soil-washing method and quantitatively assessed using the agar-film technique. Fungi were isolated and identified at two sampling times, June and October. From the isolation data obtained, it was concluded that the pattern of fungal succession in aspen litter followed the general succession schemes proposed by Garrett (1963) and Hudson (1968). The parasites on living aspen leaves included a sterile dark organism and Pleurophomella spermatiospora, while the common primary saprophytes, thought to be existing on sugars and simple carbon compounds in the leaf, included Penicillium janthinellum, Cladosporium spp., and Aureobasidium pullulans. The secondary saprophytes, such as the cellulose and lignin decomposers and associated fungi, were dominated by Penicillium syriacum, Trichoderma spp., Mucor spp., Volutella ciliata, Cylindrocarpon spp., and Phoma spp.The quantitative study revealed that the fungal length and biomass per gram dry weight of litter in each layer studied were much higher in October than in June. The amount of fungal mycelium in the humus in each of the two sampling times was not significantly different. The difference in standing crop of mycelium from June to October in the aspen LFH horizon was estimated to be at least 58 g wet mycelium per square metre of dry litter.

scholarly journals Applications of plant growth promoting bacteria and Trichoderma spp. for controlling Orobanche crenata in faba bean

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
Mahmoud Ahmed Touny El-Dabaa ◽  
Hassan Abd-El-Khair
Keyword(s):  
Plant Growth ◽  
Faba Bean ◽  
Growth Parameters ◽  
Dry Weight ◽  
Research Centre ◽  
Plant Growth Promoting Bacteria ◽  
Fresh Weight ◽  
Orobanche Crenata ◽  
Trichoderma Spp ◽  
Shoot Dry Weight

Abstract Background Orobanche crenata is an obligate root parasite belonging to Orbanchaceae. Broomrape causes great damage to the faba bean. Several attempts were applied for controlling parasitic weeds. So, the aim of this work is to study the application of Trichoderma spp. as well as three rhizobacteria species in comparison to herbicidal effect of Glyphosate (Glialka 48% WSC) for controlling broomrape infesting faba bean (Vicia faba). Materials and methods Three pot experiments were carried out in the greenhouse of the National Research Centre, Dokki, Giza, Egypt during two successive winter seasons. Trichoderma inocula were adjusted to 3.6 × 108 propagules/ml and the bacterium inocula were adjusted at 107–109 colony-forming unit (CFU)/ml. All treatments were applied, before 1 week of sowing, at rate of 50 ml per pot in experiments I and II, while 100 ml per pot in experiment III. Results Trichoderma spp. (T. harzianum, T. viride and T. vierns) as well as three rhizobacteria species (Pseudomonas fluorescens, Bacillus subtilis and Bacillus pumilus) enhanced the growth parameters in faba bean plants, i.e. shoot length, shoot fresh weight, shoot dry weight and leaf number in the first experiment when applied without O. crenata infection. In the second experiment, all bio-control could protect plants against O. crenata infection, where it had better juvenile number reduction, than glyphosate after 2 months of application. Both B. subtilis and B. pumilus had the highest reduction to juvenile fresh weight, while their effect was equal to herbicide for juvenile dry weight, respectively. The bio-control agents had high effects until the 4th month, but it was less than that of the herbicide. In experiment III, the bio-control agents could highly reduce the juvenile parameters after 2 months, as well as juvenile fresh weight and juvenile dry weight after 4 months, than the herbicide, respectively. The bio-control agents were effective until 6 months, but less than the herbicide effect. All bio-control treatments highly increased the plant growth parameters, than the herbicide. Conclusion The application of Trichoderma spp. as well as rhizobacteria species could play an important role in controlling broomrape in faba bean as a natural bioherbicide.

Lysis and biological control of Aspergillus niger by Bacillus subtilis AF 1

1996 ◽  
Vol 42 (6) ◽  
pp. 533-538 ◽  
Author(s):  
A. R. Podile ◽  
A. P. Prakash
Keyword(s):  
Biological Control ◽  
Bacillus Subtilis ◽  
Aspergillus Niger ◽  
Fungal Growth ◽  
Dry Weight ◽  
Crown Rot ◽  
Fungal Mycelium ◽  
Infested Soil ◽  
Dual Culture ◽  
Bacterial Cells

A biocontrol rhizobacterial strain of Bacillus subtilis AF 1 grown for 6 h was coinoculated with Aspergillus niger at different time intervals and microscopic observations revealed adherence of bacterial cells to the fungal mycelium. Bacterial cells multiplied in situ and colonized the mycelial surface. Growth of AF 1 resulted in damage to the cell wall, followed by lysis. AF 1 inoculation into media containing A. niger at 0, 6, and 12 h suppressed >90% fungal growth, while in 18- and 24-h cultures fungal growth inhibition was 70 and 56%, respectively, in terms of dry weight. In dual culture the fungal growth was not accompanied by formation of spores. The mycelial preparation of A. niger as principal carbon source supported the growth of B. subtilis, as much as chitin. Extracellular protein precipitate from B. subtilis culture filtrate had a significant growth-retarding effect on A. niger. Groundnut seeds bacterized with B. subtilis showed a reduced incidence of crown rot in A. niger infested soil, suggesting a possible role of B. subtilis in biological control of A. niger.Key words: mycolytic bacteria, Bacillus subtilis, Aspergillus niger, biological control.

scholarly journals Control of lettuce bottom rot by isolates of Trichoderma spp

2014 ◽  
Vol 40 (2) ◽  
pp. 141-146 ◽  
Author(s):  
Zayame Vegette Pinto ◽  
Matheus Aparecido Pereira Cipriano ◽  
Amaury da Silva dos Santos ◽  
Ludwig Heinrich Pfenning ◽  
Flávia Rodrigues Alves Patrício
Keyword(s):  
Mycelial Growth ◽  
High Capacity ◽  
Dry Weight ◽  
Second Step ◽  
Trichoderma Spp ◽  
In Vivo Experiments ◽  
Bottom Rot ◽  
Trichoderma Isolates

Bottom rot, caused by Rhizoctonia solani AG 1-IB, is an important disease affecting lettuce in Brazil, where its biological control with Trichoderma was not developed yet. The present study was carried out with the aim of selecting Trichoderma isolates to be used in the control of lettuce bottom rot. Forty-six Trichoderma isolates, obtained with baits containing mycelia of the pathogen, were evaluated in experiments carried out in vitro and in vivo in a greenhouse in two steps. In the laboratory, the isolates were evaluated for their capabilities of parasitizing and producing toxic metabolic substances that could inhibit the pathogen mycelial growth. In the first step of the in vivo experiments, the number and the dry weight of lettuce seedlings of the cultivar White Boston were evaluated. In the second step, 12 isolates that were efficient in the first step and showed rapid growth and abundant sporulation in the laboratory were tested for their capability of controlling bottom rot in two repeated experiments, and had their species identified. The majority of the isolates of Trichoderma spp. (76%) showed high capacity for parasitism and 50% of them produced toxic metabolites capable of inhibiting 60-100% of R. solani AG1-IB mycelial growth. Twenty-four isolates increased the number and 23 isolates increased the dry weight of lettuce seedlings inoculated with the pathogen in the first step of the in vivo experiments.In both experiments of the second step, two isolates of T. virens, IBLF 04 and IBLF 50, reduced the severity of bottom rot and increased the number and the dry weight of lettuce seedlings inoculated with R. solani AG1-IB. These isolates had shown a high capacity for parasitism and production of toxic metabolic substances, indicating that the in vitro and in vivo steps employed in the present study were efficient in selecting antagonists to be used for the control of lettuce bottom rot.

Aflatoxin Production in Black Currant, Blueberry and Strawberry Jams

1978 ◽  
Vol 41 (5) ◽  
pp. 344-347 ◽  
Author(s):  
O. PENSALA ◽  
A. NISKANEN ◽  
S. LINDROTH
Keyword(s):  
Yeast Extract ◽  
Raw Materials ◽  
Fungal Growth ◽  
Dry Weight ◽  
Aflatoxin Production ◽  
Fungal Mycelium ◽  
Black Currant ◽  
Initial Ph ◽  
Different Temperatures ◽  
Yeast Extract Sucrose

Unsweetened and sweetened (20 and 44% sucrose) black currant, blueberry and strawberry jams with spores of Aspergillus parasiticus NRRL 2999 were incubated at different temperatures and atmospheres for 0.5, 1, 2, and 6 months. Hyphal dry weight, pH of medium and aflatoxin production were examined. Also, the aflatoxin distribution between mold and jam layers was examined in jam with uncontrolled and controlled pH (initial pH 3.1–3.6 and 5.6 respectively) and in 20% yeast extract sucrose broth (initial pH 5.6) after 2 weeks of incubation. Aflatoxin was observed in black currant and strawberry jams stored at 22 and 30 C, but not in blueberry jam. Addition of sugar prevented production of aflatoxin in detectable amounts, although it enhanced fungal growth. Storage at 4 C resulted in a marked reduction in fungal growth. The high CO2 atmosphere prevented production of aflatoxin in detectable amounts in black currant and blueberry jams but not in strawberry jam. Raising the initial pH of the stored jam caused an increase in aflatoxin synthesis, although the amount of fungal mycelium, in contrast was reduced. Aflatoxin synthesis as a function of fungal growth was significantly weaker in the jams than in the yeast extract sucrose broth. The results imply that the jam raw materials, particularly blueberry, contain substances inhibiting production of atlatoxins. Alternatively, it is also possible that the jam materials contain only small amounts of nutrients necessary for synthesis of aflatoxin.

scholarly journals Reproducibility of suppression of Pythium wilt of cucumber by compost

2014 ◽  
Vol 23 (3) ◽  
pp. 236-245 ◽  
Author(s):  
Mauritz Vilhelm Vestberg ◽  
Sanna Kukkonen ◽  
Päivi Parikka ◽  
Dan Yu ◽  
Martin Romantschuk
Keyword(s):  
Raw Materials ◽  
Dry Weight ◽  
Shoot Height ◽  
Bacterial Diseases ◽  
Sphagnum Peat ◽  
Shoot Dry Weight ◽  
Suppressive Capacity ◽  
The Difference ◽  
Set Up

There is increasing global interest in using compost to suppress soil-borne fungal and bacterial diseases and nematodes. We studied the reproducibility of compost suppressive capacity (SC) against Pythium wilt of cucumber using nine composts produced by the same composting plant in 2008 and 2009. A bioassay was set up in a greenhouse using cucumber inoculated with two strains of Pythium. The composts were used as 20% mixtures (v:v) of a basic steam-sterilized light Sphagnum peat and sand (3:1, v:v). Shoot height was measured weekly during the 5-week experiment. At harvest, the SC was calculated as the % difference in shoot dry weight (DW) between non-inoculated and inoculated cucumbers. The SC was not affected by year of production (2008 or 2009), indicating reproducibility of SC when the raw materials and the composting method are not changed. Differences in shoot height were not as pronounced as those for shoot DW. The results were encouraging, but further studies are still needed for producing compost with guaranteed suppressiveness properties.

Factors Influencing the Rate and Duration of Grain Filling in Wheat

1977 ◽  
Vol 4 (5) ◽  
pp. 785 ◽  
Author(s):  
I Sofield ◽  
LT Evans ◽  
MG Cook ◽  
IF Wardlaw
Keyword(s):  
Growth Rate ◽  
Grain Yield ◽  
Grain Growth ◽  
Dry Weight ◽  
Grain Filling ◽  
Close Relation ◽  
Grain Number ◽  
Lag Period ◽  
The Difference ◽  
Leaf Photosynthetic Rate

Controlled-environment conditions were used to examine the effects of cultivar and of temperature and illuminance after anthesis on grain setting and on the duration and rate of grain growth. After an initial lag period, which did not differ greatly between cultivars, grain dry weight increased linearly under most conditions until final grain weight was approached. Growth rate per grain depended on floret position within the ear, varied between cultivars (those with larger grains at maturity having a faster rate), and increased with rise in temperature. With cultivars in which grain number per ear was markedly affected by illuminance, light had relatively little effect on growth rate per grain. With those in which grain number was less affected by illuminance, growth rate per grain was highly responsive to it, especially in the more distal florets. In both cases there was a close relation between leaf photosynthetic rate as influenced by illuminance, the rate of grain growth per ear, and final grain yield per ear. The duration of linear grain growth, on the other hand, was scarcely influenced by illuminance, but was greatly reduced as temperature rose, with pronounced effects on grain yield per ear. Cultivars differed to some extent in their duration of linear growth, but these differences accounted for less of the difference in final weight per grain than did those in rate of grain growth. Under most conditions the cessation of grain growth did not appear to be due to lack of assimilates.

scholarly journals Evolution of reduced pre-adult viability and larval growth rate in laboratory populations of Drosophila melanogaster selected for shorter development time

2000 ◽  
Vol 76 (3) ◽  
pp. 249-259 ◽  
Author(s):  
N. G. PRASAD ◽  
MALLIKARJUN SHAKARAD ◽  
VISHAL M. GOHIL ◽  
V. SHEEBA ◽  
M. RAJAMANI ◽  
...  
Keyword(s):  
Growth Rate ◽  
Drosophila Melanogaster ◽  
Development Time ◽  
Larval Growth ◽  
Dry Weight ◽  
Relative Reduction ◽  
Larval Growth Rate ◽  
Laboratory Populations ◽  
The Difference ◽  
Faster Development

Four large (n > 1000) populations of Drosophila melanogaster, derived from control populations maintained on a 3 week discrete generation cycle, were subjected to selection for fast development and early reproduction. Egg to eclosion survivorship and development time and dry weight at eclosion were monitored every 10 generations. Over 70 generations of selection, development time in the selected populations decreased by approximately 36 h relative to controls, a 20% decline. The difference in male and female development time was also reduced in the selected populations. Flies from the selected populations were increasingly lighter at eclosion than controls, with the reduction in dry weight at eclosion over 70 generations of selection being approximately 45% in males and 39% in females. Larval growth rate (dry weight at eclosion/development time) was also reduced in the selected lines over 70 generations, relative to controls, by approximately 32% in males and 24% in females. However, part of this relative reduction was due to an increase in growth rate of the controls populations, presumably an expression of adaptation to conditions in our laboratory. After 50 generations of selection had elapsed, a considerable and increasing pre- adult viability cost to faster development became apparent, with viability in the selected populations being about 22% less than that of controls at generation 70 of selection.

scholarly journals Consortium Growth of Filamentous Fungi and Microalgae: Evaluation of Different Cultivation Strategies to Optimize Cell Harvesting and Lipid Accumulation

Energies ◽  
2020 ◽  
Vol 13 (14) ◽  
pp. 3648
Author(s):  
Savienne M. F. E. Zorn ◽  
Cristiano E. R. Reis ◽  
Messias B. Silva ◽  
Bo Hu ◽  
Heizir F. De Castro
Keyword(s):  
Lipid Accumulation ◽  
Unsaturated Fatty Acids ◽  
Lipid Production ◽  
Synthetic Medium ◽  
Fungal Spores ◽  
Dry Weight ◽  
Biodiesel Production ◽  
Biomass Composition ◽  
Fungal Mycelium ◽  
Fourfold Increase

This study aims to evaluate the potential of consortium biomass formation between Mucor circinelloides, an oleaginous filamentous fungal species, and Chlorella vulgaris, in order to promote a straightforward approach to harvest microalgal cells and to evaluate the lipid production in the consortium system. A synthetic medium with glucose (2 g·L−1) and mineral nutrients essential for both fungi and algae was selected. Four different inoculation strategies were assessed, considering the effect of simultaneous vs. separate development of fungal spores and algae cells, and the presence of a supporting matrix aiming at the higher recovery of algae cell rates. The results were evaluated in terms of consortium biomass composition, demonstrating that the strategy using a mature fungal mycelium with a higher algae count may provide biomass samples with up to 79% of their dry weight as algae, still promoting recovery rates greater than 97%. The findings demonstrate a synergistic effect on the lipid accumulation by the fungal strain, at around a fourfold increase when compared to the axenic control, with values in the range of 23% of dry biomass weight. Furthermore, the fatty acid profile from the samples presents a balance between saturated and unsaturated fatty acids that is likely to present an adequate balance for applications such as biodiesel production.

scholarly journals Utilizing the combined antifungal potential of Trichoderma spp. and organic amendments against dry root rot of mungbean

2019 ◽  
Vol 29 (1) ◽  
Author(s):  
Anam Choudhary ◽  
Shabbir Ashraf
Keyword(s):  
Root Rot ◽  
Root Nodules ◽  
Organic Amendments ◽  
Dry Weight ◽  
Dual Culture ◽  
Trichoderma Spp ◽  
Rhizoctonia Bataticola ◽  
Neem Cake ◽  
Greenhouse Study

AbstractThe present study was carried out to evaluate the effect of bioagents and organic amendments in suppressing the dry root rot of mungbean incited by Rhizoctonia bataticola. The locally isolated pathogen and fungal biocontrol agents were identified based on morphological and molecular characterization. These identified bioagents were tested in vitro, and the highest mycelial inhibition was recorded in dual culture assay by Trichoderma harzianum (74.44%), and among organic amendments, maximum mycelial inhibition was found in neem cake (61.11%). In a greenhouse study, T. harzianum + neem cake effectively enhanced the percent germination (93.33%) and decreased the percent disease mortality (11.67%) than the other treatments. The morphological parameter like plant height (57.50 cm), dry weight (22.83 g) root nodules (51), pods/plant (58), and 100-seed weight (5.78 g) were found to be at the maximum in this combined application. Physiological pigments viz. chlorophyll (2.41 mg/g) and carotenoids (0.19 mg/g), protein content (5.85 mg/g), and leghemoglobin (11.75 mg/g) were also found to be maximum in T. harzianum + neem cake and minimum phenol content (1.41 mg/g). The study concludes that T. harzianum + neem cake can be recommended as an effective approach for the management of dry root rot of mungbean.

Diallel analysis of competition between diploid and tetraploid genotypes of Secale cereale grown at two densities

1972 ◽  
Vol 78 (2) ◽  
pp. 251-256 ◽  
Author(s):  
J. Norrington-Davies ◽  
Janice M. Hutto
Keyword(s):  
Secale Cereale ◽  
Competitive Ability ◽  
Chromosome Doubling ◽  
Diallel Analysis ◽  
Dry Weight ◽  
Inbred Lines ◽  
Pure Stand ◽  
Competitive Effects ◽  
Alpha Effect ◽  
The Difference

SUMMARYTwo inbred diploid lines of Secale cereale L. and their derived tetraploids were grown together with a commercial diploid and tetraploid in all possible pair combinations in a pot experiment. Comparisons between lines and varieties were made in respect to dry weight measured at 30 weeks.The competitive effects within mixtures were not compensatory, the commercial varieties tending to increase in dry weight more than the homozygous inbred lines decreased. This gave rise to mixture means which tended to exceed their mid-constituent values.The analysis of reciprocal differences in dry weight showed that the effects of competition between any one genotype and its associate in mixtures were constant (alpha competition), the sign and magnitude of the alpha effect being related to the pure stand values of the genotype.The mean effect of competition was to increase the difference in dry weight between genotypes by 2·108 g for each gram by which the genotypes differed when grown alone. In each case the values for genotypes with the higher dry weight increased in mixture whereas the values for those with lower dry weights decreased.Not surprisingly, the commercial varieties were found to be better competitors than the inbred lines, but of more interest was the ineffectiveness of chromosome doubling on competitive ability. Under the conditions of density and fertility reported here, the tetraploid inbred lines could not be distinguished from their diploid progenitors either in their competitive ability or in their reaction to increased density when grown in mixtures. The same is true for the tetraploid and diploid commercial varieties.

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