Dinitrogen fixation in Phaseolus vulgaris at low temperatures: interaction of temperature, growth stage, and time of inoculation
Nodulation and N2 fixation have not been reported in beans (Phaseolus vulgaris L.) below a temperature of 13 °C but, in southern Alberta, temperatures at planting may be as low as 10 °C. Two varieties of pea beans, 'Aurora' and 'Kentwood,' were inoculated at three growth stages (seeding, primary leaf horizontal, or first trifoliate leaf open) and grown at 10, 12, 14, or 16 °C. Nodulation and acetylene (C2H2) reduction occurred in both varieties at temperatures as low as 10 °C. At the lower temperatures, cold adaptability of the plant for early root growth determined the ability for nodulation and N2 fixation. At higher temperatures, plant-growth stage was a determining factor. 'Aurora' was superior to 'Kentwood' at 10 °C in nodulation, dry matter (DM), N yield, and N2 fixation because of its tolerance to low temperatures during early root growth. Inoculation with Rhizobium phaseoli at more advanced plant-growth stages decreased the time for nodulation at all four temperatures but resulted in higher yield and more N2 fixation in 'Aurora' only at 14 and 16 °C. At 10 °C, inoculation at seeding was more effective than at the other two growth stages for both varieties. Thus plant growth stages and growth temperature both determined the ability of a bean variety to support N2 fixation at various low temperatures.