Associations Between Cellular Levels of ATP and Prodigiosin Pigment Throughout the Growth Cycle of Serratia marcescens

2021 ◽  
Author(s):  
Pryce L. Haddix
Keyword(s):  
Stationary Phase ◽  
Serratia Marcescens ◽  
Stationary Phases ◽  
Growth Cycle ◽  
High Density ◽  
High Rate ◽  
Lag Phase ◽  
Positive Role ◽  
Atp Production ◽  
Density Growth

ABSTRACT Serratia marcescens is a prolific producer of the red, membrane-associated pigment prodigiosin. Earlier work has established both a positive role for prodigiosin in ATP production during population lag phase and a negative role during high-rate, low cell density growth. This study uses the growth rate and growth phase modulation afforded by chemostat culture to extend prodigiosin functional analysis to the high density and stationary phases. Cellular levels of prodigiosin were positively associated with cellular levels of ATP during high-density growth, and artificial pigment induction during this phase increased cellular ATP. Following peak high density ATP per cell, early stationary phase enabled significant population growth while prodigiosin levels remained high and ATP declined. During late stationary phase, ATP per cell was positively associated with prodigiosin per cell while both declined during continued growth. These results provide correlational evidence for multiple effects of prodigiosin pigment on ATP production throughout the growth cycle. Earlier work and the data presented here enable formulation of a working model for the oscillating relationships between cellular levels of ATP and prodigiosin during batch culture.

scholarly journals Changes in the transport of mitochondrial Ca2+ during the culture growth cycle of Tetrahymena pyriformis

1985 ◽  
Vol 77 (1) ◽  
pp. 47-56
Author(s):  
Y.V. Kim ◽  
LYu Kudzina ◽  
V.P. Zinchenko ◽  
Y.V. Evtodienko
Keyword(s):  
Stationary Phase ◽  
Morphological Changes ◽  
Stationary Phases ◽  
Mitochondrial Protein ◽  
Tetrahymena Pyriformis ◽  
Growth Cycle ◽  
Lag Phase ◽  
Rapid Release ◽  
Culture Growth ◽  
Energy Dependent

The properties of the Ca2+ transport system of mitochondria, isolated in various phases of growth of static cultures of Tetrahymena pyriformis, were studied. A large increase in the endogenous energy-dependent Ca2+ content of mitochondria was observed as cultures of T. pyriformis passed through the exponential and stationary phases of growth (approx. 0.25 and 50 nmol Ca2+ per mg mitochondrial protein, respectively). Simultaneously, the mitochondria dramatically lost their ability to withstand large concentrations of Ca2+ and ADP. However, in the latter case they were able to phosphorylate a large amount of ADP if the strong Ca2+ chelator, ethylene glycol bis-(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid, was initially present in the incubation medium. Furthermore, all the changes observed in mitochondria from the stationary phase cells were completely reversed when cell proliferation was re-activated after the lag phase, either by reseeding the stationery cells in fresh growth medium or by oxygenation of the old medium. In aerobic conditions even a small addition of Ca2+ was able to induce rapid release of Ca2+ from mitochondria isolated during the stationary phase of growth. It is suggested that the redistribution of Ca2+ between the mitochondria and the cytoplasm at the onset of the lag phase may serve as the main trigger for the subsequent biochemical and morphological changes observed in T. pyriformis.

GROWTH STUDIES ON ARTHROBACTER GLOBIFORMIS: II. CHANGES IN MACROMOLECULAR LEVELS DURING GROWTH

1962 ◽  
Vol 8 (5) ◽  
pp. 655-661 ◽  
Author(s):  
I. L. Stevenson
Keyword(s):  
Stationary Phase ◽  
Fold Increase ◽  
Growth Cycle ◽  
Phase Cell ◽  
Lag Phase ◽  
Differential Rate ◽  
Protein Ratio ◽  
Stationary Phase Culture ◽  
Protein Levels ◽  
Dna And Protein Synthesis

Changes in macromolecular levels (RNA, DNA, protein) have been followed during the growth cycle of A. globiformis. When a stationary phase culture is transferred to fresh medium a 12-fold increase in RNA level and 6-fold increases in DNA and protein levels are observed during the predivisional lag phase. Initially RNA synthesis precedes DNA and protein synthesis but all reach the same differential rate 2 to 3 hours prior to division. During the predivisional lag period the RNA/protein ratio per cell expands from 0.19 to 0.36. Once division occurs, cells of A. globiformis remain in the enlarged pleomorphic form until the medium becomes limiting; at this time synthesis of macromolecules ceases and the continued division (three to four generations) results in progressively smaller cells until the coccoid stationary phase cell-type is reached.

scholarly journals Kinetic Analysis of Growth Rate, ATP, and Pigmentation Suggests an Energy-Spilling Function for the Pigment Prodigiosin of Serratia marcescens

2008 ◽  
Vol 190 (22) ◽  
pp. 7453-7463 ◽  
Author(s):  
Pryce L. Haddix ◽  
Sarah Jones ◽  
Pratik Patel ◽  
Sarah Burnham ◽  
Kaori Knights ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Serratia Marcescens ◽  
Growth Rates ◽  
High Growth ◽  
Atp Synthesis ◽  
Opportunistic Pathogen ◽  
Growth Conditions ◽  
Atp Production ◽  
Concentration Changes ◽  
Energy Spilling

ABSTRACTSerratia marcescensis a gram-negative environmental bacterium and opportunistic pathogen.S. marcescensexpresses prodigiosin, a bright red and cell-associated pigment which has no known biological function for producing cells. We present here a kinetic model relating cell, ATP, and prodigiosin concentration changes forS. marcescensduring cultivation in batch culture. Cells were grown in a variety of complex broth media at temperatures which either promoted or essentially prevented pigmentation. High growth rates were accompanied by large decreases in cellular prodigiosin concentration; low growth rates were associated with rapid pigmentation. Prodigiosin was induced most strongly during limited growth as the population transitioned to stationary phase, suggesting a negative effect of this pigment on biomass production. Mathematically, the combined rate of formation of biomass and bioenergy (as ATP) was shown to be equivalent to the rate of prodigiosin production. Studies with cyanide inhibition of both oxidative phosphorylation and pigment production indicated that rates of biomass and net ATP synthesis were actually higher in the presence of cyanide, further suggesting a negative regulatory role for prodigiosin in cell and energy production under aerobic growth conditions. Considered in the context of the literature, these results suggest that prodigiosin reduces ATP production by a process termed energy spilling. This process may protect the cell by limiting production of reactive oxygen compounds. Other possible functions for prodigiosin as a mediator of cell death at population stationary phase are discussed.

scholarly journals Production of prodigiosin pigment by Serratia marcescens is negatively associated with cellular ATP levels during high-rate, low-cell-density growth

2020 ◽  
Vol 66 (3) ◽  
pp. 243-255 ◽  
Author(s):  
Pryce L. Haddix ◽  
Robert M.Q. Shanks
Keyword(s):  
Growth Rate ◽  
Serratia Marcescens ◽  
Cell Density ◽  
Chemostat Culture ◽  
High Rate ◽  
Phase Cell ◽  
Intact Cells ◽  
Atp Levels ◽  
Density Growth ◽  
Low Cell Density

Serratia marcescens is a facultatively anaerobic bacterium and the most recognized producer of the hydrophobic pigment prodigiosin. Previous work has shown that prodigiosin both increases ATP production during population lag phase and approximately doubles the stationary-phase cell yield. Here, we employed both batch and chemostat culture methods to investigate prodigiosin’s role during high rate growth at low cell density as peak cellular ATP levels decline. Batch culture experiments utilizing artificial pigment induction showed an ATP reduction during low cell density growth. In addition, pigment induction during fixed growth rate chemostat culture revealed a negative correlation between cellular levels of prodigiosin and ATP (r = −0.95). Variable growth rate chemostat experiments showed an inverse relationship between ATP per cell and prodigiosin per cell during low-density growth but a direct relationship during high-density growth. Rate modeling of chemostat data quantified the pigment’s effect on cellular levels of ATP for both population growth phases. Finally, prodigiosin production in a heterologous bacterium led to ATP decline. These data with intact cells complement the established in vitro proton import function of prodigiosin pigment and may indicate an energy-spilling function during high rate, low cell density growth.

Changes of trehalose content and expression of relative genes during the bioethanol fermentation bySaccharomyces cerevisiae

2016 ◽  
Vol 62 (10) ◽  
pp. 827-835 ◽  
Author(s):  
Chenfeng Yi ◽  
Fenglian Wang ◽  
Shijun Dong ◽  
Hao Li
Keyword(s):  
Phase Transition ◽  
Stationary Phase ◽  
Ethanol Tolerance ◽  
Stationary Phases ◽  
Exponential Phase ◽  
Yeast Cells ◽  
Ethanol Stress ◽  
Lag Phase ◽  
Significant Difference ◽  
Trehalose Content

Traditionally, trehalose is considered as a protectant to improve the ethanol tolerance of Saccharomyces cerevisiae. In this study, to clarify the changes and roles of trehalose during the bioethanol fermentation, trehalose content and expression of related genes at lag, exponential, and stationary phases (i.e., 2, 8, and 16 h of batch fermentation process) were determined. Although yeast cells at exponential and stationary phase had higher trehalose content than cells at lag phase (P < 0.01), there was no significant difference in trehalose content between exponential and stationary phases (P > 0.05). Moreover, expression of the trehalose degradation-related genes NTH1 and NTH2 decreased at exponential phase in comparison with that at lag phase; compared with cells at lag phase, cells at stationary phase had higher expression of TPS1, ATH1, NTH1, and NTH2 but lower expression of TPS2. During the lag–exponential phase transition, downregulation of NTH1 and NTH2 promoted accumulation of trehalose, and to some extent, trehalose might confer ethanol tolerance to S. cerevisiae before stationary phase. During the exponential–stationary phase transition, upregulation of TPS1 contributed to accumulation of trehalose, and Tps1 protein might be indispensable in yeast cells to withstand ethanol stress at the stationary phase. Moreover, trehalose would be degraded to supply carbon source at stationary phase.

scholarly journals Inositol transport in Saccharomyces cerevisiae is regulated by transcriptional and degradative endocytic mechanisms during the growth cycle that are distinct from inositol-induced regulation.

1996 ◽  
Vol 7 (1) ◽  
pp. 81-89 ◽  
Author(s):  
K S Robinson ◽  
K Lai ◽  
T A Cannon ◽  
P McGraw
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Stationary Phase ◽  
Exponential Growth ◽  
Growth Cycle ◽  
Endocytic Pathway ◽  
Negative Regulator ◽  
Lag Phase ◽  
Free Medium ◽  
Uptake Activity ◽  
Inositol Uptake

Regulation of inositol uptake activity in Saccharomyces cerevisiae during the growth cycle was examined. Activity increased as the cell population transited from lag phase to exponential growth, and continued to increase until late exponential phase. The increase in activity was due to increased transcription of the ITR1 gene and synthesis of the Itr1 permease. When the culture reached stationary phase, uptake activity decreased and dropped to a minimum within 4 h. The decrease was due to repression of ITR1 transcription, independent of the negative regulator Opi1p, and degradation of the existing permease. Degradation depended on delivery of the permease to the vacuole through the END3/END4 endocytic pathway. During exponential growth in inositol-containing medium the permease is also rapidly degraded, whereas in inositol-free medium the permease is highly stable. Rapid degradation of the permease at stationary phase occurred in inositol-free medium, indicating that there are two distinct mechanisms that trigger endocytosis and degradation in response to different physiological stimuli. In addition, the level of the enzyme required for inositol biosynthesis, inositol-1-phosphate synthase, encoded by INO1, is not reduced in stationary-phase cells, and this contrast in the regulation of inositol supply is discussed.

scholarly journals Temporal Analysis of Coxiella burnetii Morphological Differentiation

2004 ◽  
Vol 186 (21) ◽  
pp. 7344-7352 ◽  
Author(s):  
Sherry A. Coleman ◽  
Elizabeth R. Fischer ◽  
Dale Howe ◽  
David J. Mead ◽  
Robert A. Heinzen
Keyword(s):  
Stationary Phase ◽  
Coxiella Burnetii ◽  
Time Course ◽  
Growth Cycle ◽  
Cycle Phase ◽  
Lag Phase ◽  
Developmental Cycle ◽  
Phase Lag ◽  
Electron Microscopic ◽  
Transmission Electron

ABSTRACT Coxiella burnetii undergoes a poorly defined developmental cycle that generates morphologically distinct small-cell variants (SCV) and large-cell variants (LCV). We developed a model to study C. burnetii morphogenesis that uses Vero cells synchronously infected with homogeneous SCV (Nine Mile strain in phase II) harvested from aged infected cell cultures. A time course transmission electron microscopic analysis over 8 days of intracellular growth was evaluated in conjunction with one-step growth curves to correlate morphological differentiations with growth cycle phase. Lag phase occurred during the first 2 days postinfection (p.i.) and was primarily composed of SCV-to-LCV morphogenesis. LCV forms predominated over the next 4 days, during which exponential growth was observed. Calculated generation times during exponential phase were 10.2 h (by quantitative PCR assay) and 11.7 h (by replating fluorescent focus-forming unit assay). Stationary phase began at approximately 6 days p.i. and coincided with the reappearance of SCV, which increased in number at 8 days p.i. Quantitative reverse transcriptase-PCR demonstrated maximal expression of scvA, which encodes an SCV-specific protein, at 8 days p.i., while immunogold transmission electron microscopy revealed degradation of ScvA throughout lag and exponential phases, with increased expression observed at the onset of stationary phase. Collectively, these results indicate that the overall growth cycle of C. burnetii is characteristic of a closed bacterial system and that the replicative form of the organism is the LCV. The experimental model described in this report will allow a global transcriptome and proteome analysis of C. burnetii developmental forms.

Carboxylated cyclofructan 6 as a hydrolytically stable high efficiency stationary phase for hydrophilic interaction liquid chromatography and mixed mode separations

2016 ◽  
Vol 8 (31) ◽  
pp. 6038-6045 ◽  
Author(s):  
Yadi Wang ◽  
M. Farooq Wahab ◽  
Zachary S. Breitbach ◽  
Daniel W. Armstrong
Keyword(s):  
Liquid Chromatography ◽  
Benzoic Acid ◽  
Stationary Phase ◽  
Mixed Mode ◽  
High Efficiency ◽  
Stationary Phases ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Hydrophilic Interaction

Stationary phases composed of native cyclofructan 6 (CF6) and benzoic acid modified CF6 were synthesized and evaluated for hydrophilic interaction liquid chromatography (HILIC).

Noncontingent Nonreinforcement of a Response as the Aversive Event in a Cer Paradigm

1971 ◽  
Vol 29 (3_suppl) ◽  
pp. 1196-1198 ◽  
Author(s):  
Richard S. Calef ◽  
Richard A. Kaufman ◽  
Ronald N. Bone ◽  
Steven A. Werk
Keyword(s):  
Reinforcement Schedule ◽  
Present Experiment ◽  
High Density ◽  
High Rate ◽  
Aversive Event ◽  
Necessary Condition ◽  
Facilitation Effect ◽  
Response Facilitation ◽  
Early Training ◽  
Low Rate

The present experiment investigated the effects of noncontingent nonreinforcement as the aversive event in a CER paradigm. The results showed a significant response-facilitation effect during early training, but none during later training with a high rate-producing, high-density reinforcement schedule. The present results imply that a low rate-producing, high-density reinforcement schedule is not a necessary condition for response facilitation.

Carbon dioxide requirement of Neisseria gonorrhoeae growing on a solid medium

1976 ◽  
Vol 4 (2) ◽  
pp. 129-132
Author(s):  
D J Platt
Keyword(s):  
Carbon Dioxide ◽  
Neisseria Gonorrhoeae ◽  
Rapid Growth ◽  
Solid Medium ◽  
Colony Growth ◽  
Growth Cycle ◽  
Lag Phase ◽  
Replica Plating ◽  
Early Stages ◽  
Male Patients

A survey of 120 isolations of Neisseria gonorrhoeae from male patients showed that 47 (39%) isolates were unable to produce visible colonies without a supply of exogenous carbon dioxide. Of 63 strains, 25 strains required CO2 for isolation but none exhibited a CO2 requirement beyond subculture 4. CO2-requiring strains deprived of CO2 appeared to lose their colony-forming ability exponentially in an environment otherwise conductive to growth. The CO2 requirement was found to be linked to the initiation of growth. An agar-to-agar replica-plating device was used to study the early stages of colonial growth. The CO2 requirement was also found to correlate with the various phases of the colony growth cycle, such that it was required during lag phase, not required during the phase of rapid growth, and returned as colonies aged. These results are discussed.

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