Transforming growth factor-β (TGF-β) activation in cutaneous wounds after topical application of aloe vera gel

2016 ◽  
Vol 94 (12) ◽  
pp. 1285-1290 ◽  
Author(s):  
Nasrin Takzaree ◽  
Abbas Hadjiakhondi ◽  
Gholamreza Hassanzadeh ◽  
Mohammad Reza Rouini ◽  
Azadeh Manayi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wound Healing ◽  
Transforming Growth Factor ◽  
Skin Diseases ◽  
Healing Process ◽  
Aloe Vera ◽  
Cell Counting ◽  
Control Group ◽  
Aloe Vera Gel ◽  
Experimental Group

Aloe vera is a medicinal plant used to treat various skin diseases. The effects of using aloe vera gel on the healing process were investigated by microscopic methods, cell counting, and TGF-β gene expression in the wound bed. Sixty Wistar rats weighing 200–250 g were placed under anesthesia in sterile conditions. A square 1.5 cm × 1.5 cm wound was made on the back of the neck. The rats were divided into control and 2 experimental groups. Additionally, the control and experimental groups were separated into 3 subgroups corresponding to 4, 7, and 14 days of study. In the first experimental group, aloe vera was used twice on the wound. The second experimental group received aloe vera overtreatment once on the wound. The positive control group received daily application of 1% phenytoein cream following surgical wound creation. The control group did not receive any treatment. This tissue was examined using histological staining (H&E) and Masson’s Trichrome. Wound surface and wound healing were evaluated separately. TGF-β gene expression was analyzed by RT–PCR. Results showed that fibroblasts in both experimental groups were significantly increased, thereby acceleration wound healing. Application of aloe vera gel will increase TGF-β gene expression, ultimately accelerating the wound healing process.

scholarly journals The possibility of polymorphonuclear leukocyte activation in dental socket healing by freeze-dried Aloe vera induction

2021 ◽  
Vol 54 (3) ◽  
pp. 124
Author(s):  
Pratiwi Soesilawati ◽  
Ester Arijani Rachmat ◽  
Ira Arundina ◽  
Nita Naomi
Keyword(s):  
Wound Healing ◽  
Tooth Extraction ◽  
Healing Process ◽  
Aloe Vera ◽  
Inflammatory Cells ◽  
Control Group ◽  
Wound Healing Process ◽  
Control Group Design ◽  
Aloe Vera Gel ◽  
Freeze Dried

Background: Research has proved that Aloe vera has anti-inflammatory, anti-bacterial, and immunomodulator properties that can accelerate the healing process. These properties could be beneficial in the tooth extraction wound-healing process. Neutrophils are the first inflammatory cells to invade and are the predominant cell markers in the wound for 24 hours after injury. Polymorphonuclear leukocytes (PMN) provide initial protection against micro-organisms, as they engulf and digest foreign bodies. Purpose: The aim of this study was to prove the effect of Aloe vera 90% gel application on the PMN count in the healing process of Cavia cobaya following tooth extraction. Methods: This laboratory experimental research was done using Post-Test Only Control Group Design. The lower incisive of twelve male Cavia cobayas were extracted then divided into four groups. Group P1 and P3 had 90% Aloe vera gel applied into the socket and sutured; group K1 and K3, received no 90% Aloe vera gel application and acted as controls. Samples from group P1 and K1 were terminated and had their mandibula taken one day after tooth extraction, and those from group P3 and K3 were terminated after three days. Tissues were then processed into histology slides, and PMN cells were counted. Results: There were significant differences in the PMN count between groups K1, P1, and K3 compared with P3 (p < 0.05). Higher PMN count was shown on the group containing Aloe vera 90% gel compared with control group, both on day one and three after tooth extraction. Conclusion: Aloe vera 90% gel can increase the PMN count in the inflammation phase of the tooth extraction wound-healing process.

Transforming growth factor-? isoforms differently stimulate pro?2 (I) collagen gene expression during wound healing process in transgenic mice

2002 ◽  
Vol 190 (3) ◽  
pp. 375-381 ◽  
Author(s):  
Takuro Kinbara ◽  
Fumiaki Shirasaki ◽  
Shigeru Kawara ◽  
Yutaka Inagaki ◽  
Benoit de Crombrugghe ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wound Healing ◽  
Growth Factor ◽  
Transgenic Mice ◽  
Transforming Growth Factor ◽  
Healing Process ◽  
Wound Healing Process ◽  
Collagen Gene ◽  
Collagen Gene Expression

scholarly journals PENGARUH PEMBERIAN GEL KOMBINASI EKSTRAK GETAH PEPAYA (Carica papaya L) DAN EKSTRAK DAGING LIDAH BUAYA (Aloe vera) TERHADAP PROSES PENYEMBUHAN ULKUS TRAUMATIKUS PADA MALE WISTAR RATS YANG MENDERITA DIABETES MELITUS

2015 ◽  
Vol 2 (1) ◽  
pp. 41
Author(s):  
Umi Kulsum ◽  
Ratnawati Hendari ◽  
Siti Chumaeroh
Keyword(s):  
Wound Healing ◽  
Ulcer Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Healing Process ◽  
Aloe Vera ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Papaya Latex

Introduction: Research on the use of papaya and aloevera sap on wound healing each been done, but effects the combination of the two extracts on wound healing in diabetes mellitus condition has not been done.Purpose: This study aims to determine the effect of a gel combination of papaya latex and aloevera flesh extracts on the traumatics ulcer healing process in diabetic rats.Methods: True experimental laboratory research with the post-test-only control group design that performed on 28 male wistar rats which were divided intofour groups: DM and non-DM rat that treatment with povidone iodine and gel combination of papaya latex and aloevera flesh extract. The treatment was done for 7 days with smearing that was given 2x/day. Traumatics ulcer healing seen from the number of fibroblasts that then were analyzed by One WayAnova and post hoc LSD test.Results: The results showed the number of fibroblasts in DM and non-DM rat with povidone iodine were 44.6 and 42.73; in the DM and non-DM rat with a gel combination of papaya latex and aloe vera flesh extracts were 61.10 and 77.03. One Way Anova test resulted p-value = 0.000, it was mean that there were at least a significant difference on the number of fibroblasts in the two groups. Differences of the number fibroblasts was shown in all groups, except in the group DM and non-DM rats with povidone iodine (p = 0.764).Conclusion: Conclusion of the study was the provision of a gel combination of papaya latex and aloevera flesh extracts effect on the traumatics ulcer healing process in diabetic rats.

scholarly journals Silk derived formulations for accelerated wound healing in diabetic mice

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10232
Author(s):  
Muniba Tariq ◽  
Hafiz Muhammad Tahir ◽  
Samima Asad Butt ◽  
Shaukat Ali ◽  
Asma Bashir Ahmad ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silk Fibroin ◽  
Chronic Wounds ◽  
Histological Analysis ◽  
Healing Process ◽  
Aloe Vera ◽  
Wound Contraction ◽  
Wound Dressings ◽  
Control Group ◽  
Diabetic Mice

Background The present study aimed to prepare effective silk derived formulations in combination with plant extract (Aloe vera gel) to speed up the wound healing process in diabetic mice. Methods Diabetes was induced in albino mice by using alloxan monohydrate. After successful induction of diabetes in mice, excision wounds were created via biopsy puncture (6 mm). Wound healing effect of silk sericin (5%) and silk fibroin (5%) individually and in combination with 5% Aloe vera gel was evaluated by determining the percent wound contraction, healing time and histological analysis. Results The results indicated that the best biocompatible silk combination was of 5% silk fibroin and 5% Aloe vera gel in which wounds were healed in 13 days with wound contraction: 98.33 ± 0.80%. In contrast, the wound of the control group (polyfax) healed in 19 day shaving 98.5 ± 0.67% contraction. Histological analysis revealed that the wounds which were treated with silk formulations exhibited an increased growth of blood vessels, collagen fibers, and much reduced inflammation. Conclusion It can be concluded that a combination of Bombyx mori silk and Aloe vera gel is a natural biomaterial that can be utilized in wound dressings and to prepare more innovative silk based formulations for speedy recovery of chronic wounds.

scholarly journals Collagen extract obtained from Nile tilapia (Oreochromis niloticus L.) skin accelerates wound healing in rat model via up regulating VEGF, bFGF, and α-SMA genes expression

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Zizy I. Elbialy ◽  
Ayman Atiba ◽  
Aml Abdelnaby ◽  
Ibrahim I. Al-Hawary ◽  
Ahmed Elsheshtawy ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Rat Model ◽  
Nile Tilapia ◽  
Transforming Growth Factor ◽  
Healing Process ◽  
Control Group ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Genes Expression

Abstract Background Collagen is the most abundant structural protein in the mammalian connective tissue and represents approximately 30% of animal protein. The current study evaluated the potential capacity of collagen extract derived from Nile tilapia skin in improving the cutaneous wound healing in rats and investigated the underlying possible mechanisms. A rat model was used, and the experimental design included a control group (CG) and the tilapia collagen treated group (TCG). Full-thickness wounds were conducted on the back of all the rats under general anesthesia, then the tilapia collagen extract was applied topically on the wound area of TCG. Wound areas of the two experimental groups were measured on days 0, 3, 6, 9, 12, and 15 post-wounding. The stages of the wound granulation tissues were detected by histopathologic examination and the expression of vascular endothelial growth factor (VEGF), and transforming growth factor (TGF-ß1) were investigated using immunohistochemistry. Moreover, relative gene expression analysis of transforming growth factor-beta (TGF-ß1), basic fibroblast growth factor (bFGF), and alpha-smooth muscle actin (α-SMA) were quantified by real-time qPCR. Results The histopathological assessment showed noticeable signs of skin healing in TCG compared to CG. Immunohistochemistry results revealed remarkable enhancement in the expression levels of VEGF and TGF-β1 in TCG. Furthermore, TCG exhibited marked upregulation in the VEGF, bFGF, and α-SMA genes expression. These findings suggested that the topical application of Nile tilapia collagen extract can promote the cutaneous wound healing process in rats, which could be attributed to its stimulating effect on recruiting and activating macrophages to produce chemotactic growth factors, fibroblast proliferation, and angiogenesis. Conclusions The collagen extract could, therefore, be a potential biomaterial for cutaneous wound healing therapeutics.

scholarly journals The Effect of Aloe vera (Linn) On Cutaneous Wound Healing and Wound Contraction Rate in Adult Rabbits

2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Martha Orendu Attah ◽  
Tonye Watson Jacks ◽  
Attah Jacob ◽  
Otong Eduitem ◽  
Barnabas John
Keyword(s):  
Wound Healing ◽  
Aloe Vera ◽  
Wound Contraction ◽  
Control Group ◽  
Cutaneous Wound Healing ◽  
Contraction Rate ◽  
Wound Area ◽  
Cutaneous Wound ◽  
Cutaneous Wounds ◽  
Aloe Vera Gel

Background: In the present research study, the rate of cutaneous wound healing and contraction rate in healthy rabbits using Aloe vera pulp was studied.Methods: Ten healthy rabbits were used for the study. They were divided into two groups consisting of five rabbits each. Cutaneous wounds were made on the lumbar region of each rabbit using a template which ensured that the wounds were of the same size in all the rabbits. 5ml of Aloe vera gel was applied to the wounds of the animals in the test group, while nothing was applied to the wound area of the animals in the control group. The wound area in each group was measured for a period of 21 days, using a venire caliper and tracing paper which was used to trace the wound area. Tissue samples were removed from the wound area in both experimental and control groups and subjected to routine histological analysis, also, morphometric analysis was performed.Results: The rate of wound contraction and mean centripetal contraction was calculated in both groups and graphically represented using Microsoft Excel. The results showed that animals who were treated with Aloe vera gel had a greater wound contraction rate, as well as rapid wound closure. The micrographs showed a thicker epithelial layer, with thinner collagen fibers in the dermis of experimental animals compared to the control group. There was also an abundant capillary bed at the dermal-epidermal junction in the experimental group, compared to the control group.Conclusion:  Aloe vera may increase the rate of wound healing by accelerating epithelial migration, and may also play a role in neo-vascularization of the newly healed area.Keywords: Aloe vera, Cutaneous Wounds, Rabbits, Wound Contraction, Wound Healing

scholarly journals THE EFFECT OF ALOE VERA TOWARD THE NUMBER OF FIBROBLASTS ON THE WOUND INCISION OF WISTAR RAT’S (RATUS NORVEGICUS)

2020 ◽  
Vol 1 (1) ◽  
pp. 30
Author(s):  
Chusnul Nur Fatmawati ◽  
Ekowati Retnanin ◽  
Tavip Dwi Wahyuni
Keyword(s):  
Wound Healing ◽  
Transforming Growth Factor ◽  
Healing Process ◽  
Aloe Vera ◽  
T Test ◽  
Field Of View ◽  
Wound Healing Process ◽  
Wistar Strain ◽  
Alternative Choice ◽  
Group 1

Introduction: Incision is a wound caused by a sharp object, for example in surgery. The characteristics of the wound are pain, open wounds, and the length of the wound is greater than in it. Handling the wound there are various ways, one of which is using Aloe Vera. The aim of this study was to determine the effect of topical Aloe vera gel concentration of 10%, 20%, and 40% to determine the amount of fibroblast tissue in wistar strain rats (Rattus orvegicus) in incisions. Methods: This study uses an experimental design with a sample of 75 rats divided into 5 groups; 0.9% NaCl group, 1% tulle-framycetin sulfate group, 10% Aloe vera group, 20% Aloe vera group and 40% Aloe vera group. Samples of incisional wound skin were performed on days 3.7, and 12. All data were processed and analyzed statistically using SPSS 20. Based on microscopic observations at 10 times per field of view, the highest number of fibroblasts given at NaCl was given on the day 12th is 28.98 per field of view, treatment using tulle-framycetin sulfate 1% is 34.5 per field of view, treatment using Aloe vera 10% is 17.32 per field of view, treatment using Aloe vera 20% is 33, 9 per field of view while the treatment using Aloe vera was 40% ie 18.7 per view. Results : The results of the study with the Independent T-test and Paired T-test showed that the results of fibroblast tissue between 0.9% NaCl group, 1% tulle-framycetin sulfate, 10% Aloe vera, 40% Aloe vera with 20% Aloe vera there were differences which were significant (p <0.05) where 20% Aloe vera gave effective results in the wound healing process. Conclusion : This study concluded that 20% of Aloe vera can be used as an alternative choice of wound healing. In the next study it is expected to examine the variable TGF (Transforming Growth Factor) that affects cell growth in wound healing.

A Study to Evaluate the Effectiveness of Sitz Bath on Episiotomy Wound Healing among Postnatal Mothers in Aravindan Hospital at Coimbatore

2021 ◽  
pp. 281-286
Author(s):  
Kalaivani L.
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Sitz Bath ◽  
Highly Effective ◽  
Postnatal Mothers ◽  
Level Of Significance ◽  
And Control ◽  
Experimental Group

Statement of the problem: ‘A study to evaluate the effectiveness of sitz bath on episiotomy wound healing among postnatal mothers in Aravindan hospital at Coimbatore. Objectives of the study: 1. To assess the episiotomy wound healing among postnatal mothers both in experimental and control group. 2. To administer Sitz Bath Application to mothers in Experimental Group. 3. To evaluate the effectiveness of sitz bath on episiotomy wound healing among postnatal mothers in experimental group. 4. To compare the effectiveness of sitz bath on episiotomy wound healing among postnatal mothers between experimental and control group. 5. To find out the association between the post observational episiotomy wound healing scores of postnatal with selected demographic variable. Methodology: The quantitative approach - quasi-experimental, non- equivalent control group pre-test. Post-test design was selected to evaluate the effectiveness of sitzbath on Episiotomy wound healing among postnatal mothers The researcher adopted non probability convenient sampling technique and 60 postnatal mothers were selected for the study from Aravindan hospital Coimbatore. They are divided into two groups, 30 in experimental group and 30 in control group. The investigator used Reeda Scale and visual Analog pain scale to assess the episiotomy wound healing. Results: The findings reveal that application of sitz bath is highly effective on severe redness (63.3%), severe oedema (876%), severe ecchymosis (83.3%), severe discharge (93.3%) and poor approximation of wound (90%). Posttest on 5th day revealed that they had moderate redness (30%), moderate oedema (34.3%), mild ecchymosis (27.6%), moderate discharge (38.6%) and improved approximation of wound (27.6%). For all parameters of REEDA calculated t-value >p-value, which infers that application of sitz bath is statistically highly effective for healing of episiotomy at 0.05 level of significance. The calculated t-value is 22 > 2.05, which infers that application of sitz bath is statistically highly effective for healing of episiotomy wound at 0.05 level of significance. The application of sitz bath is highly effective in experimental group based on REEDA parameters as per redness (45%) in experimental group when compared to control group redness (90%), discharge (38.6) in experimental group when compared to control group discharge (53.3%), edema (34%) in experimental group when compared to control group odema. 6%), ecchymoseis 7.6%) in experimental group when compared to control group ecchymosis (53.3%) and on approximation of wound (27.6%) when compared to control group approximation of wound(47.6%). Hence H2 is accepted. Conclusion: This information shows that Application of Sitz Bath is effective in episiotomy wound healing process

scholarly journals Effect of Photobiomodulation Therapy on Oxidative Stress Markers in Healing Dynamics of Diabetic Neuropathic Wounds in Wistar Rats

2021 ◽  
Author(s):  
Gagana Karkada ◽  
G. Arun Maiya ◽  
Praveen Arany ◽  
Mohandas Rao ◽  
Shalini Adiga ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Wound Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Photobiomodulation Therapy ◽  
Control Group ◽  
Sciatic Nerve Crush ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Experimental Group

Abstract Background Prolonged and overlapping phases of wound healing in diabetes are mainly due to the redox imbalance resulting in the chronicity of the wound. Photobiomodulation therapy works on the principle of absorption of photon energy and its transduction into a biological response in the living tissue. It alleviates the cellular responses, thereby improving the mechanism of wound healing in diabetes. Objective To find out the effect of photobiomodulation therapy of dosage 4 J/cm2 in the healing dynamics of diabetic neuropathic wounds in Wistar rats and its relation with oxidative stress markers. Methodology Diabetes was induced using Streptozotocin of 60 mg/kg of body weight to eighteen female Wistar rats. Neuropathy was induced by the sciatic nerve crush injury followed by an excisional wound of 2 cm2 on the back of the animal. Experimental group animals were treated with dosage 4 J/cm2 of wavelength 655 and 808 nm, and control group animals were kept unirradiated. The biomechanical, histopathological, and biochemical changes were analysed in both groups. Results There was a reduction in mean wound healing time and an increased rate of wound contraction in the experimental group animals compared to its control group. The experimental group showed improved redox status, and histopathological findings revealed better proliferative cells, keratinisation, and epithelialization than un-irradiated controls. Conclusions Photobiomodulation therapy of dosage 4 J/cm2 enhanced the overall wound healing dynamics of the diabetes-induced neuropathic wound and optimised the oxidative status of the wound, thereby facilitating a faster healing process.

scholarly journals A comparative study between aloe vera gel dressing and conventional dressing in chronic wounds

2017 ◽  
Vol 4 (10) ◽  
pp. 3427
Author(s):  
Virendra S. Athavale ◽  
Shivmurti N. Khandalkar ◽  
Megha Mahawar ◽  
Iresh Shetty ◽  
Aditya Lad
Keyword(s):  
Wound Healing ◽  
Surface Area ◽  
Chronic Wounds ◽  
Average Rate ◽  
Aloe Vera ◽  
Cost Effective ◽  
Control Group ◽  
Percentage Reduction ◽  
Aloe Vera Gel ◽  
Group A

Background: The aim of this study was to evaluate the effectiveness and rate of healing of Aloe vera gel in treatment of chronic wounds, to compare the effectiveness of Aloe vera gel with conventional dressing (normal saline and povidone iodine) and to assess the percentage reduction of wound healing with Aloe vera gel dressing.Methods: The study was conducted at Dr. D. Y. Patil Medical College and Hospital, DPU University, for a period of 2 months (from January 2017 to March 2017) and is a prospective and comparative randomized type of study using 50 cases (Group A and Group B, 25 each). The study was approved by the Institute’s Ethics Committee.Results: Data analysis showed that at the end of 4 weeks, mean surface area in Group A is reduced to 4.58 cm2 from 9.79 cm2 which is higher than that in the control group. Also, the average rate of healing in Aloe vera gel is more than control group. Percentage reduction in ulcer surface area was calculated to be much more in Group A as compared to Group B.Conclusions: The study concluded that Aloe vera gel is highly effective in treatment of chronic ulcers and stimulates the growth of wound healing. Thus, reducing the hospital stay. Apart from being efficacious in wound healing, Aloe vera gel is safe product. No allergic reactions/infections were associated with Aloe vera gel. Aloe vera gel not only heals faster but is also cost effective.

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