scholarly journals THE EFFECT OF ALOE VERA TOWARD THE NUMBER OF FIBROBLASTS ON THE WOUND INCISION OF WISTAR RAT’S (RATUS NORVEGICUS)

2020 ◽  
Vol 1 (1) ◽  
pp. 30
Author(s):  
Chusnul Nur Fatmawati ◽  
Ekowati Retnanin ◽  
Tavip Dwi Wahyuni
Keyword(s):  
Wound Healing ◽  
Transforming Growth Factor ◽  
Healing Process ◽  
Aloe Vera ◽  
T Test ◽  
Field Of View ◽  
Wound Healing Process ◽  
Wistar Strain ◽  
Alternative Choice ◽  
Group 1

Introduction: Incision is a wound caused by a sharp object, for example in surgery. The characteristics of the wound are pain, open wounds, and the length of the wound is greater than in it. Handling the wound there are various ways, one of which is using Aloe Vera. The aim of this study was to determine the effect of topical Aloe vera gel concentration of 10%, 20%, and 40% to determine the amount of fibroblast tissue in wistar strain rats (Rattus orvegicus) in incisions. Methods: This study uses an experimental design with a sample of 75 rats divided into 5 groups; 0.9% NaCl group, 1% tulle-framycetin sulfate group, 10% Aloe vera group, 20% Aloe vera group and 40% Aloe vera group. Samples of incisional wound skin were performed on days 3.7, and 12. All data were processed and analyzed statistically using SPSS 20. Based on microscopic observations at 10 times per field of view, the highest number of fibroblasts given at NaCl was given on the day 12th is 28.98 per field of view, treatment using tulle-framycetin sulfate 1% is 34.5 per field of view, treatment using Aloe vera 10% is 17.32 per field of view, treatment using Aloe vera 20% is 33, 9 per field of view while the treatment using Aloe vera was 40% ie 18.7 per view. Results : The results of the study with the Independent T-test and Paired T-test showed that the results of fibroblast tissue between 0.9% NaCl group, 1% tulle-framycetin sulfate, 10% Aloe vera, 40% Aloe vera with 20% Aloe vera there were differences which were significant (p <0.05) where 20% Aloe vera gave effective results in the wound healing process. Conclusion : This study concluded that 20% of Aloe vera can be used as an alternative choice of wound healing. In the next study it is expected to examine the variable TGF (Transforming Growth Factor) that affects cell growth in wound healing.

scholarly journals Polymer-Based Scaffolds Loaded with Aloe vera Extract for the Treatment of Wounds

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 961
Author(s):  
Sibusiso Alven ◽  
Vuyolwethu Khwaza ◽  
Opeoluwa O. Oyedeji ◽  
Blessing A. Aderibigbe
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Mechanical Performance ◽  
Healing Process ◽  
Aloe Vera ◽  
Antimicrobial Properties ◽  
Biological Properties ◽  
Wound Management ◽  
Wound Healing Process ◽  
Treatment Of Wounds

The treatment of wounds is one challenging biomedical field due to delayed wound healing common in chronic wounds. Several factors delay wound healing, including microbial infections, malnutrition, underlying physiological conditions, etc. Most of the currently used wound dressing materials suffer from poor antimicrobial properties, poor biodegradability and biocompatibility, and weak mechanical performance. Plant extracts, such as Aloe vera, have attracted significant attention in wound management because of their interesting biological properties. Aloe vera is composed of essential constituents beneficial for the wound healing process, such as amino acids, vitamins C and E, and zinc. Aloe vera influences numerous factors that are involved in wound healing and stimulates accelerated healing. This review reports the therapeutic outcomes of aloe vera extract-loaded polymer-based scaffolds in wound management.

Transforming growth factor-? isoforms differently stimulate pro?2 (I) collagen gene expression during wound healing process in transgenic mice

2002 ◽  
Vol 190 (3) ◽  
pp. 375-381 ◽  
Author(s):  
Takuro Kinbara ◽  
Fumiaki Shirasaki ◽  
Shigeru Kawara ◽  
Yutaka Inagaki ◽  
Benoit de Crombrugghe ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wound Healing ◽  
Growth Factor ◽  
Transgenic Mice ◽  
Transforming Growth Factor ◽  
Healing Process ◽  
Wound Healing Process ◽  
Collagen Gene ◽  
Collagen Gene Expression

scholarly journals The effect of earthworm extract on promoting skin wound healing

2018 ◽  
Vol 38 (2) ◽  
Author(s):  
Zhen-han Deng ◽  
Jian-jian Yin ◽  
Wei Luo ◽  
Ronak Naveenchandra Kotian ◽  
Shan-shan Gao ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healthcare System ◽  
Transforming Growth Factor ◽  
Healing Process ◽  
White Blood Cells ◽  
Skin Wound ◽  
Wound Healing Process ◽  
Blood Capillary ◽  
Skin Wound Healing

Chronic nonhealing wounds pose a significant challenge to healthcare system because of its tremendous utilization of resources and time to heal. It has a well-deserved reputation for reducing the quality of life for those affected and represent a substantial economic burden to the healthcare system overall. Earthworms are used as a traditional Chinese medicine, and have been applied pharmacologically and clinically since a long time in China. However, there is paucity in data regarding its wound healing effects. Therefore, we investigated the effect of earthworm extract (EE) on skin wound healing process. The obtained data showed that EE has healing effects on local wound of mice. It decreased the wound healing time and reduced the ill-effects of inflammation as determined by macroscopic, histopathologic, hematologic, and immunohistochemistry parameters. The potential mechanism could be accelerated hydroxyproline and transforming growth factor-β secretion—thus increasing the synthesis of collagen, promoting blood capillary, and fibroblast proliferation. It could accelerate the removal of necrotic tissue and foreign bodies by speeding up the generation of interleukin-6, white blood cells, and platelets. It thus enhances immunity, reduces the risk of infection, and promotes wound healing. All in all, the obtained data demonstrated that EE improves quality of healing and could be used as a propitious wound healing agent.

scholarly journals The extracellular matrix: an active or passive player in fibrosis?

2011 ◽  
Vol 301 (6) ◽  
pp. G950-G955 ◽  
Author(s):  
Thomas N. Wight ◽  
Susan Potter-Perigo
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Transforming Growth Factor ◽  
Healing Process ◽  
Transforming Growth Factor Β ◽  
Cytokine Signaling ◽  
Wound Healing Process ◽  
Inflammatory Stimuli ◽  
Specialized Form ◽  
Excessive Accumulation

Fibrosis is characterized by excessive accumulation of collagen and other extracellular matrix (ECM) components, and this process has been likened to aberrant wound healing. The early phases of wound healing involve the formation of a provisional ECM containing fibrin, fibrinogen, and fibronectin. Fibroblasts occupy this matrix and proliferate in response to activators elaborated by leukocytes that have migrated into the wound and are retained by the ECM. This coincides with the appearance of the myofibroblast, a specialized form of fibroblast whose differentiation is primarily driven by cytokines, such as transforming growth factor-β (TGF-β), and by mechanical tension. When these signals are reduced, as when TGF-β secretion is reduced, or as in scar shrinkage, myofibroblasts undergo apoptosis, resulting in a collagen-rich, cell-poor scar. Retention of myofibroblasts in fibrosis has been described as the result of imbalanced cytokine signaling, especially with respect to levels of activated TGF-β. ECM components can regulate myofibroblast persistence directly, since this phenotype is dependent on extracellular hyaluronan, tenascin-C, and the fibronectin splice variant containing the “extra domain A,” and also, indirectly, through retention of TGF-β-secreting cells such as eosinophils. Thus the ECM is actively involved in both cellular and extracellular events that lead to fibrosis. Targeting components of the ECM as cells respond to injury and inflammatory stimuli holds promise as a means to avoid development of fibrosis and direct the wound-healing process toward reestablishment of a healthy equilibrium.

Myostatin-null mice exhibit delayed skin wound healing through the blockade of transforming growth factor-β signaling by decorin

2012 ◽  
Vol 302 (8) ◽  
pp. C1213-C1225 ◽  
Author(s):  
Chen Zhang ◽  
Chek Kun Tan ◽  
Craig McFarlane ◽  
Mridula Sharma ◽  
Nguan Soon Tan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Wound Repair ◽  
Transforming Growth Factor ◽  
Healing Process ◽  
Critical Role ◽  
Transforming Growth Factor Β ◽  
Skin Wound ◽  
Wound Healing Process ◽  
Skin Wound Healing

Myostatin (Mstn) is a secreted growth and differentiation factor that belongs to the transforming growth factor-β (TGF-β) superfamily. Mstn has been well characterized as a regulator of myogenesis and has been shown to play a critical role in postnatal muscle regeneration. Herein, we report for the first time that Mstn is expressed in both epidermis and dermis of murine and human skin and that Mstn-null mice exhibited delayed skin wound healing attributable to a combination of effects resulting from delayed epidermal reepithelialization and dermal contraction. In epidermis, reduced keratinocyte migration and protracted keratinocyte proliferation were observed, which subsequently led to delayed recovery of epidermal thickness and slower reepithelialization. Furthermore, primary keratinocytes derived from Mstn-null mice displayed reduced migration capacity and increased proliferation rate as assessed through in vitro migration and adhesion assays, as well as bromodeoxyuridine incorporation and Western blot analysis. Moreover, in dermis, both fibroblast-to-myofibroblast transformation and collagen deposition were concomitantly reduced, resulting in a delayed dermal wound contraction. These decreases are due to the inhibition of TGF-β signaling. In agreement, the expression of decorin, a naturally occurring TGF-β suppressor, was elevated in Mstn-null mice; moreover, topical treatment with TGF-β1 protein rescued the impaired skin wound healing observed in Mstn-null mice. These observations highlight the interplay between TGF-β and Mstn signaling pathways, specifically through Mstn regulation of decorin levels during the skin wound healing process. Thus we propose that Mstn agonists might be beneficial for skin wound repair.

Influence of Aloe Vera Gel on Dermal Wound Healing Process in Rat

2008 ◽  
Vol 19 (1) ◽  
pp. 73-77 ◽  
Author(s):  
Nasrin Takzare ◽  
Mir-jamal Hosseini ◽  
Gholamreza Hasanzadeh ◽  
Hamideh Mortazavi ◽  
Alireza Takzare ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Aloe Vera ◽  
Wound Healing Process ◽  
Dermal Wound Healing ◽  
Aloe Vera Gel ◽  
Dermal Wound

scholarly journals Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1122
Author(s):  
Nutda Sutthammikorn ◽  
Volaluck Supajatura ◽  
Hainan Yue ◽  
Miho Takahashi ◽  
Sunee Chansakaow ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Therapeutic Option ◽  
Healing Process ◽  
Unmet Need ◽  
New Drugs ◽  
Wound Healing Process ◽  
Diabetic Mice ◽  
Diabetic Wounds

Nonhealing wounds are major socioeconomic challenges to healthcare systems worldwide. Therefore, there is a substantially unmet need to develop new drugs for wound healing. Gynura procumbens, a herb found in Southeast Asia, may be an effective therapeutic for nonhealing diabetic wounds. The aim of this study was to evaluate the efficacy of G. procumbens on wound healing in the diabetic milieu. G. procumbens extract was obtained using 95% ethanol and its components were determined by thin layer chromatography. Diabetes was induced in mice using streptozotocin. We found that G. procumbens extract contained stigmasterol, kaempferol and quercetin compounds. Topical application of G. procumbens on the wounded skin of diabetic mice accelerated wound healing and induced the expression of angiogenin, epidermal growth factor, fibroblast growth factor, transforming growth factor and vascular endothelial growth factor. Furthermore, G. procumbens promoted in vitro wound healing and enhanced the migration and/or proliferation of human endothelial cells, fibroblasts, keratinocytes and mast cells cultured in diabetic conditions. Finally, G. procumbens promoted vascular formation in the diabetic mice. To the best of our knowledge, this is the first study that evaluates in vivo wound healing activities of G. procumbens and activation of cells involved in wound healing process in diabetic conditions. The findings that G. procumbens accelerates wound healing and activates cells involved in the wound healing process suggest that G. procumbens might be an effective alternative therapeutic option for nonhealing diabetic wounds.

scholarly journals Application of pomegranate (Punica granatum Linn.) fruit extract for accelerating post-tooth extraction wound healing

2018 ◽  
Vol 51 (4) ◽  
pp. 189
Author(s):  
Intan Nirwana
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Treatment Group ◽  
Tooth Extraction ◽  
Transforming Growth Factor ◽  
Healing Process ◽  
Punica Granatum ◽  
Control Group ◽  
Wound Healing Process ◽  
Significant Difference

Background: Trauma occurring during tooth extraction can cause complications such as bleeding, infection, fracture and dry socket and constitutes an inflammatory response trigger. Pomegranate (Punica granatum Linn.) extract, which contains large amounts of punicallagin and ellagic acid, possesses various qualities, including; anti-inflammatory, anti-bacterial and anti-oxidant. Pomegranate extract can inhibit proinflammatory cytokine production, while also suppressing inflammation response thereby accelerating wound healing. Purpose: This study aimed to analyze the effect of pomegranate extract application to the tooth extraction wounds of Cavia cobaya (C. cobaya) on the expression of fibroblast growth factor-2 (FGF-2) and transforming growth factor β (TGF-β) on the fourth day of the wound-healing process. Methods: This study used 12 C. cobaya, divided into two groups, namely; control and treatment. The subjects were anesthetized, before their lower left central incisor was extracted and the entire socket filled with CMC-Na 3% in members of the control group and pomegranate extract in those of the treatment group. The twelve C. cobaya were sacrificed on day 4, their lower jaw subsequently being removed and decalcified for approximately 30 days. The mandibula tissue was stained using a immunohistochemical technique. FGF-2 and TGF-β were used to evaluate the healing process in the extracted tooth socket. Differences in the expression of FGF-2 and TGF-β were evaluated statistically by means of a t-test. Results: This study indicated a significant difference between the control and the treatment groups (p<0.05). The treatment group members whose sockets were filled with pomegranate extract showed high FGF-2 and TGF-β expression. Conclusion: This study confirmed that the administration of pomegranate extract to post-extraction tooth wounds of C. cobaya increases the expression of FGF-2 and TGF-β on day 4, thereby accelerating the wound healing process.

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