Genetic identification of 43 elite clonal accessions of Populus deltoides by SSR fingerprinting

2016 ◽  
Vol 96 (3) ◽  
pp. 494-502 ◽  
Author(s):  
Hailin Liu ◽  
Wanxu Yang ◽  
Jing Hou ◽  
Nan Hu ◽  
Tongming Yin ◽  
...  
Keyword(s):  
Populus Deltoides ◽  
Genetic Discrimination ◽  
Genetic Identification ◽  
Specific Gene ◽  
Gene Introgression ◽  
Average Value ◽  
Ssr Loci ◽  
Polymorphic Bands ◽  
Relationship Of ◽  
Ssr Fingerprinting

In this study, 43 elite clones of Populus deltoides were fingerprinted with ABI 3730 capillary electrophoresis by using six SSR primer pairs. Based on the fingerprinting profiles, 62 polymorphic bands were generated with a mean number of 10 alleles per locus, and allele numbers amplified by each primer in these clones ranged from 5 (NJFUP-poly10) to 16 (NJFUP-poly07). The power of discrimination values for these primer pairs ranged from 0.80 to 0.94, with an average value of 0.89. Among the six primer pairs, the most efficient primer pair for genetic discrimination of these elite clones was NJFUP-poly02, which could identify 22 of the 43 elite clones directly. In conclusion, all the 43 elite clones of P. deltoides could be discriminated unambiguously based on their genotypes at the six SSR loci. The combination of all six loci gave considerable reliability and accuracy for genetic identification of these clonal accessions. Subsequently, genetic relationship of these clones was plotted by the UPGMA clustering and principle component analysis. Results of both analyses indicated that clone “C7-1” had the largest genetic distance from the other clones, followed by clone “C51-1” and a subgroup comprising clone “C100-3” and “C62-3”. These clones are proposed to be possibly more affected by the inter-specific gene introgression.

THE EFFECT OF EXTRACURRICULAR ACTIVITY TOWARD ENGLISH LEARNING ACHIEVEMENT OF NURSING STUDENTS

2020 ◽  
Vol 9 (2) ◽  
pp. 183
Author(s):  
Juliana Juliana ◽  
Novi Afrianti
Keyword(s):  
Nursing Students ◽  
Extracurricular Activities ◽  
English Language ◽  
Extracurricular Activity ◽  
English Learning ◽  
Learning Achievement ◽  
Average Value ◽  
Strongly Agree ◽  
Relationship Of ◽  
Banda Aceh

This research is a descriptive correlative study. The purpose of this study was to determine the effect and relationship of English extracurricular activities (English Club) on the English learning achievement of nursing students. This research took place at the Academy of Nursing Kesdam Iskandar Muda Banda Aceh. The population in this study were all students of the Academy of Nursing Kesdam Iskandar Muda Banda Aceh. The samples are students of 2018/2019 who take part in English extracurricular activities as many as 50 students. Data collection methods in this study are the documentation and the questionnaire. Documentation is used to obtain data about the names and number of students who become research respondents. The questionnaire was used to find out students' opinions about English extracurricular activities. The data was analysed in term of percentage by using statistic descriptive SPSS 16.0 and Ms Excel 2013. The results of this study are the researchers found that the average value of students' perceptions reached 82.3% which means students strongly agree with the existence of English language extracurricular activities to support the improvement of their English learning achievement. The data is strengthened by the results of documentation of students' English grades. From the results of the analysis showed that English extracurricular activities proved to have an effect on students' English learning achievement.

scholarly journals On Newcomb's method of investigating periodicities and its application to Brückner's weather cycle

1914 ◽  
Vol 90 (619) ◽  
pp. 349-355
Keyword(s):  
Thermal Radiation ◽  
The Other ◽  
New Method ◽  
Critical Examination ◽  
Numerical Work ◽  
Average Value ◽  
Other Hand ◽  
Relationship Of ◽  
The Relationship ◽  
Great Simplicity

During the last few years of his life Prof. Simon Newcomb was keenly interested in the problem of periodicities, and devised a new method for their investigation. This method is explained, and to some extent applied, in a paper entitled "A Search for Fluctuations in the Sun's Thermal Radiation through their Influence on Terrestrial Temperature." The importance of the question justifies a critical examination of the relationship of the older methods to that of Newcomb, and though I do not agree with his contention that his process gives us more than can be obtained from Fourier's analysis, it has the advantage of great simplicity in its numerical work, and should prove useful in a certain, though I am afraid, very limited field. Let f ( t ) represent a function of a variable which we may take to be the time, and let the average value of the function be zero. Newcomb examines the sum of the series f ( t 1 ) f ( t 1 + τ) + f ( t 2 ) f ( t 2 + τ) + f ( t 3 ) f ( t 3 + τ) + ..., where t 1 , t 2 , etc., are definite values of the variable which are taken to lie at equal distances from each other. If the function be periodic so as to repeat itself after an interval τ, the products are all squares and each term is positive. If, on the other hand, the periodic time be 2τ, each product will be negative and the sum itself therefore negative. It is easy to see that if τ be varied continuously the sum of the series passes through maxima and minima, and the maxima will indicated the periodic time, or any of its multiples.

scholarly journals Genotyping oil flax varieties using the microsatellite DNA marker system

2020 ◽  
Vol 21 (5) ◽  
pp. 531-539
Author(s):  
S. Z. Guchetl ◽  
T. A. Tchelyustnikova
Keyword(s):  
Information Content ◽  
Genetic Relatedness ◽  
Polymorphic Information Content ◽  
Biotic And Abiotic Stresses ◽  
Crop Acreage ◽  
Marker System ◽  
Average Value ◽  
Ssr Loci ◽  
New Varieties ◽  
Microsatellite Dna Marker

The tendency to increase crop acreage of oil flax requires the development of new varieties with high indicators of ecological plasticity, productivity and resistance to biotic and abiotic stresses. The application of modern biotechnolog ical approaches based on the use of molecular markers can accelerate the assessment of genetic differences and the dete rmination of potential of the source material for breeding. The research was aimed at assessment of the genotyping parameters of some oil flax varieties of VNIIMK breeding using the system of microsatellite markers. Seventeen variety samples of flax were used as the material for the research. DNA was isolated using CTAB buffer. Eleven SSR loci were used for the identification of varieties. Ten polymorphic loci were identified during the research. The total number of counted alleles is 32. The size of alleles varied in the range of 111-210 bps. The number of alleles per locus ranged from 2 to 6 with an average value of 3.20. The value of the index of polymorphic information content (PIC) was from 0.29 to 0.75 with an average parameter value of 0.51. The effective number of alleles for different loci is determined in the range of 1.40-3.94 with an average value of 2.28. The level of information content of the marker system (PIC 0.51) corresponds to that for identifying sets of genotypes from collections with a limitation in geographical origin. There were established differences in the frequency of occurrence of alleles. The discriminatory potential of the used marker system allowed to identify 15 variety samples. Two genotypes with common origin were identical. The degree of genetic relatedness of the studied flax genotypes has been evaluated. The obtained results will serve as the basis for the subsequent construction of genetic passports of oil flax varieties of VNIIMK breeding.

scholarly journals Genetic diversity among flue-cured tobacco cultivars on the basis of AFLP markers

2009 ◽  
Vol 45 (No. 4) ◽  
pp. 155-159 ◽  
Author(s):  
X.Z. Liu ◽  
C.S. He ◽  
Y.M. Yang ◽  
H.Y. Zhang
Keyword(s):  
Genetic Diversity ◽  
Yunnan Province ◽  
Southwest China ◽  
Germplasm Conservation ◽  
Research Centre ◽  
Aflp Data ◽  
Breeding Research ◽  
Polymorphic Bands ◽  
Relationship Of ◽  
The Relationship

AFLP analyses were used to assess the genetic similarity among selected accessions at the South China Tobacco Breeding Research Centre (Yunnan province, Southwest China). 154 AFLP polymorphic fragments out of 561 fragments were used to assess the genetic diversity among 28 tobacco accessions. The average number of polymorphic bands per AFLP primer pair was 15.4. AFLPs seemed to be an effective classification tools for germplasm conservation and breeding. Limited genetic variation was detected within this group of accessions. The relationship of cultivars was estimated by cluster analysis based on AFLP data.

scholarly journals Identificación de cadáveres sometidos a altas temperaturas, a partir de las características macroscópicas de sus órganos dentales y la aplicabilidad de la genética forense / Identification of Bodies Exposed to High Temperatures Based on Macroscopic...

2016 ◽  
Vol 35 (74) ◽  
Author(s):  
María Del Socorro Barraza Salcedo ◽  
Martha Leonor Rebolledo Cobos
Keyword(s):  
High Temperatures ◽  
Dental Pulp ◽  
Literature Search ◽  
Forensic Genetics ◽  
Forensic Odontology ◽  
Genetic Identification ◽  
Dental Tissues ◽  
Relationship Of ◽  
The Relationship ◽  
Forensic Genetic

<p><strong>ABSTRACT. </strong><em><strong>Background:</strong></em> Forensic dentistry in cases of incineration provides scientific elements that allow the identification of bodies, by analyzing dental organs, through the isolation of DNA obtained from the pulp as an alternative to confirm the identity of the victim. When the degree of temperature is highly elevated, dental tissues are vulnerable and therefore the DNA pulp is not salvageable, wasting resources and time by lack of standards to identify macroscopic characteristics that indicate this situation reliably. <em><strong>Objective:</strong></em> To describe the main features of teeth subjected to high temperatures and the relationship of these to the viability of their DNA, as a contribution to forensic genetic identification. <em><strong>Methods:</strong></em> A literature search for three databases were used; Pubmed, EBSCO and Google academician, 50 articles were selected in several languages, which made regarding the descriptors "cremated teeth; violence; Forensic Odontology; ID; DNA samples; dental pulp". <em><strong>Results:</strong></em> Most authors reported the resistance of dental tissues when subjected to high temperatures and others showed the success of the identification through dental comparisons. <em><strong>Conclusions:</strong></em> A successful identification evidenced by DNA extracted from bone and dental remains. The literature reviewed suggests that up to certain temperatures, can be carried out identification by forensic genetics of cremated victims by DNA contained in dental pulp. Strict national referencing was observed in the physical characteristics or incinerated dental macroscopic bodies.</p>

Relationship of resting metabolic rate to body composition and protein turnover

1990 ◽  
Vol 258 (6) ◽  
pp. E990-E998 ◽  
Author(s):  
S. Welle ◽  
K. S. Nair
Keyword(s):  
Protein Synthesis ◽  
Metabolic Rate ◽  
Protein Turnover ◽  
Resting Metabolic Rate ◽  
Whole Body ◽  
Simultaneous Estimation ◽  
Total Body Potassium ◽  
Average Value ◽  
Total Body ◽  
Relationship Of

This study examined whether variability among healthy young adults in resting metabolic rate, normalized for the amount of metabolically active tissue (assessed by total body potassium), is related to protein turnover. Resting metabolic rate was measured by indirect calorimetry for 2 h in 26 men and 21 women, 19-33 yr old, with simultaneous estimation of protein turnover during a 4-h infusion of L-[1-13C]leucine. After adjusting metabolic rate for total body potassium, the standard deviation was only 89 kcal/day, or 5.5% of the average value. There was a high correlation between leucine flux (an index of proteolysis) and metabolic rate (r = 0.84) and between the nonoxidized portion of leucine flux (an index of protein synthesis) and metabolic rate (r = 0.83). This relationship was weaker, but still significant, after adjusting leucine metabolism and metabolic rate for total body potassium (r = 0.36 for leucine flux vs. metabolic rate, r = 0.33 for nonoxidized portion of leucine flux vs. metabolic rate, P less than 0.05). The regression analysis suggested that the contribution of protein turnover to resting metabolic rate was approximately 20% in an average subject. Metabolic rate and protein turnover were highest in the subjects with the greatest amount of body fat, even after accounting for differences in whole body potassium. Neither resting metabolic rate nor protein turnover was related to total or free concentrations of thyroxine or triiodothyronine, within the euthyroid range.(ABSTRACT TRUNCATED AT 250 WORDS)

Genomic diversity and relationship of Bacillus thuringiensis and Bacillus cereus by multi-REP-PCR fingerprinting

2007 ◽  
Vol 53 (3) ◽  
pp. 343-350 ◽  
Author(s):  
Ameur Cherif ◽  
Besma Ettoumi ◽  
Noura Raddadi ◽  
Daniele Daffonchio ◽  
Abdellatif Boudabous
Keyword(s):  
Bacillus Thuringiensis ◽  
Bacillus Cereus ◽  
Genetic Relationship ◽  
Genomic Diversity ◽  
Consensus Sequences ◽  
Pcr Fingerprinting ◽  
Polymorphic Bands ◽  
Relationship Of ◽  
Type Strains ◽  
Group Similarity

The genomic diversity and relationship among 56 Bacillus thuringiensis and Bacillus cereus type strains were investigated by multi-REP-PCR fingerprinting consisting of three PCR reactions targeting the enterobacterial ERIC1 and ERIC2 and the streptococcal BOXA1R consensus sequences. A total of 113 polymorphic bands were generated in the REP-PCR profiles that allowed tracing of a single dendrogram with three major groups. Bacillus cereus strains clustered together in the A and B groups. Most of the B. thuringiensis strains clustered in group C, which included groups of serovars with a within-group similarity higher than 40% as follows: darmstadiensis, israelensis, and morrisoni; aizawai, kenyae, pakistani, and thompsoni; canadensis, entomocidus, galleriae, kurstaki, and tolworthi; alesti, dendrolimus, and kurstaki; and finitimus, sotto, and thuringiensis. Multi-REP-PCR fingerprinting clustered B. thuringiensis serovars in agreement with previously developed multilocus sequence typing schemes, indicating that it represents a rapid shortcut for addressing the genetic relationship of unknown strains with the major known serovars.

scholarly journals THE RELATIONSHIP OF NITRATE AND PHOSPHATE WITH ABUNDANCE OF EPIPELIC IN THE WATERS OF SUNGAITOHOR VILLAGE, REGENCY OF MERANTI ISLANDS, RIAU PROVINCE

2020 ◽  
Vol 3 (3) ◽  
pp. 225-235
Author(s):  
Ongki Okto Beranda ◽  
Bintal Amin ◽  
Sofyan Husein Siregar
Keyword(s):  
Community Structure ◽  
Correlation Coefficient ◽  
Water Area ◽  
Dominance Index ◽  
Average Value ◽  
A Value ◽  
Epipelic Diatoms ◽  
Index Value ◽  
Relationship Of ◽  
The Relationship

The study was conducted in August-October 2019. Aims to determine theconcentration of nitrate, phosphate, uniformity index, diversity and index of dominancy,abundance of epipelic diatoms and know the relationship between nitrate,phosphate and epipelic abundance diatoms in the water area of Sungaitohor DesaMeranti of Riau Islands district. The method used in this research is a surveymethod consisting of 4 research stations. Location of observation and sampling is in the waters of Muara Sungaitohor Kabupaten Meranti Islands. Basedon the results of this study of nitrateconcentrations in the waters of Sungaitohor Bekisar village 0,068-0,092 mg/l, phosphate concentrations ranged0,097-0,220 mg/L. The Epipelic diatom found in the water area of Sungaitohorvillage amounted to 14 types. As for the abundance of type diatomsepipelics ranged from 27,531.72– 40,531.72 ind/cm2. From theCalculation of the community structure diatoms epipelic obtained diversityindex value (H ') which indicates a low index value of h ' has a value of2.7527 and is at a range of 1 ≤ H ' ≤ 3. The uniformity index (E) indicatesthat an epipelic spread is not balanced with an average value of 0.3643uniformity and is at a range of value ≤ 0.5. Meanwhile, the Dominance index (D)shows the value of 0.1704 where the value of the area in the waters ofSungaitohor village does not dominate. The relationship of abundance ofepipelic diatoms with the concentration of nitrate is positive and very weak,with a correlation coefficient of R = 0.0030. While the relationship ofAbundance diatomic epipelic relationship with phosphate concentrations isnegative and weak, with a correlation coefficient r = 0.262. Abundance ofdiatomic epipelics is directly proportional to the concentration of nitrate andinversely proportional to phosphate concentrations

scholarly journals Assessment of Genetic Diversity and Population Structure of Tunisian Barley Accessions (Hordeum vulgare L.) Using SSR Markers

2020 ◽  
Vol 73 (4) ◽  
Author(s):  
Salem Marzougui ◽  
Mohamed Kharrat ◽  
Mongi Ben Younes
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Polymorphic Information Content ◽  
Hordeum Vulgare L ◽  
Software Analysis ◽  
Average Value ◽  
Allele Number ◽  
Cultivar Development ◽  
Principal Coordinates ◽  
Ssr Loci

In barley breeding programs, information about genetic dissimilarity and population structure is very important for genetic diversity conservation and new cultivar development. This study aimed to evaluate the genetic variation in Tunisian barley accessions (<em>Hordeum</em><em> </em><em>vulgare </em>L.) based on simple sequence repeat (SSR). A total of 89 alleles were detected at 26 SSR loci. The allele number per locus ranged from two to five, with an average of 3.4 alleles per locus detected from 32 barley accessions, and the average value of polymorphic information content was 0.45. A cluster analysis based on genetic similarity was performed, and the 32 barley resources were classified into five groups. Principal coordinates (PCoA) explained 12.5% and 9.3% of the total variation, and the PCoA was largely consistent with the results of cluster separation of STRUCTURE software analysis. The analysis of genetic diversity in barley collection will facilitate cultivar development and effective use of genetic resources.

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