scholarly journals Nutrient sufficiency levels for haskap (Lonicera caeruleaL.) using the boundary-line approach

2019 ◽  
Vol 99 (2) ◽  
pp. 268-280 ◽  
Author(s):  
Ekene Mark-Anthony Iheshiulo ◽  
Lord Abbey ◽  
Andrew M. Hammermeister
Keyword(s):  
Growth Rate ◽  
Nova Scotia ◽  
Leaf Tissue ◽  
Principal Component ◽  
Leaf Size ◽  
Nutrient Status ◽  
Boundary Line ◽  
Tissue Samples ◽  
Leaf Tissues ◽  
Representative Farms

Adequate supply of plant nutrients is crucial for haskap plant growth and increased productivity. A study was carried out to determine the variability in haskap (Lonicera caerulea L. cv. Indigo Gem) plant characteristics in relation to soil and leaf tissue nutrient status. A total of 19 composite soil samples and corresponding plant leaf tissue samples were collected in 2016 from 12 locations in Nova Scotia. Plant parameters measured include growth rate, leaf size, leaf chlorophyll content, and visual observations. A boundary-line approach was used to determine nutrient sufficiency ranges in leaf tissues of 2.23%–2.96% for N, 0.22%–0.28% for P, 0.84%–1.32% for K, 1.63%–2.10% for Ca, and 0.14%–0.50% for Mg. Principal component and correlation analysis suggested a possible antagonistic interaction between leaf K and Mg. Negative associations were observed most frequently between Ca and Mg and other nutrients, especially K. Plant parameters such as bush volume, leaf size, and growth rate were closely related to soil and leaf K. Deficiencies in leaf tissue K and P were identified as potentially important factors limiting growth. Therefore, there is a need to adjust or balance the application of these nutrients. In conclusion, the sufficiency ranges derived can be used as a guiding principle in diagnosing the nutritional status of haskap cv. Indigo Gem on representative farms in Nova Scotia.

Enhanced production of falcarinol-type polyacetylenes in hairy roots of cultivated carrot (Daucus carota subsp. sativus)

2021 ◽  
Author(s):  
Frank Dunemann ◽  
Christoph Böttcher
Keyword(s):  
Higher Plants ◽  
Leaf Tissue ◽  
Fold Increase ◽  
Biological Functions ◽  
Wild Type ◽  
Tissue Samples ◽  
Positive Effects ◽  
Enhanced Production ◽  
Leaf Tissues

Abstract Polyacetylenes (PAs) are a large group of bioactive phytochemicals, which are primarily produced by higher plants of the families Apiaceae and Araliaceae. Especially aliphatic C17-polyacetylenes of the falcarinol-type such as falcarinol (FaOH) and falcarindiol (FaDOH) are known for their numerous biological functions including positive effects on human health. In this study we investigate the potential of carrot hairy root (HR) cultures for accumulation of PAs in vitro. Three individual plants of seven differently colored carrot cultivars were used for the development of HR cultures by transformation of root discs with the wild-type Rhizobium rhizogenes strain 15834. A total of 51 individual HR lines were established and quantitatively analysed by HPLC/DAD together with root, petiole and leaf tissue samples for their FaOH and FaDOH levels. Among the five tissues sampled from the donor plants, root periderm samples generally exhibited the highest PA levels with FaDOH as prevailing PA and large differences between cultivars. Compared with the periderm, FaOH levels were highly increased in HRs of all investigated carrot cultivars, whereas the FaDOH levels were not significantly increased. Considering the low to moderate PA concentration in root and leaf tissues of the orange cultivars there was an up to 30-fold increase of the FaOH concentration in HRs derived from orange genotypes. In this study, we demonstrate, that carrot HRs are able to produce large amounts of PAs. Hence, we suggest that HRs might be a suited tool to assess the function of candidate genes involved in the biosynthesis of major PAs.

scholarly journals Potential nutrient-response curves and sufficiency ranges of ‘Grande Naine’ banana cultivated in two environment

2021 ◽  
Vol 43 (1) ◽  
Author(s):  
Vagner Alves Rodrigues Filho ◽  
Júlio César Lima Neves ◽  
Sérgio Luiz Rodrigues Donato ◽  
Bruno Vinícius Castro Guimarães
Keyword(s):  
Nutritional Status ◽  
Relative Yield ◽  
Leaf Tissue ◽  
Reference Population ◽  
Nutrient Concentrations ◽  
Boundary Line ◽  
Response Curves ◽  
Data Set ◽  
Tissue Samples ◽  
Nutrient Response

Abstract Adequate plant nutrition is essential to attain higher yields. The objective was to determine potential-nutrient response curves and sufficiency ranges using the boundary line approach and balance indices of Kenworthy for interpreting the nutritional status of ‘Grand Nain’ banana cultivated in two environments. The study was carried out using a database containing leaf nutrient concentrations and yields of bananas cultivated on two areas located in Missão Velha-CE, and Ponto Novo-BA, Brazil. Plots with high-yielding plants, which were those with yields above average plus 0.5 standard deviation, were used atem as reference population. The database was subdivided into two sets. One of them contained 46 leaf tissue samples and reference population with yield greater than 58.84 Mg ha-1 year-1, in Missão Velha-CE. The second data set contained 19 samples and reference population with yield greater than 76.12 Mg ha-1 year-1 in Ponto Novo-BA. Potential response curves were fitted to the relationship between relative yield and leaf element concentrations and balance indices of Kenworthy. Models expressed high predictive power. Sufficiency ranges for macro- and micronutrient concentrations and balance indices of Kenworthy were established. The ranges allow an improved nutritional status assessment of irrigated ‘Grand Nain’ bananas.

scholarly journals Evaluation of Systemic Imidacloprid and Herbicide Treatments on Flatheaded Borer (Coleoptera: Buprestidae) Management in Field Nursery Production

2020 ◽  
Vol 113 (6) ◽  
pp. 2808-2819
Author(s):  
Karla M Addesso ◽  
Jason B Oliver ◽  
Nadeer N Youssef ◽  
Donna C Fare
Keyword(s):  
Leaf Tissue ◽  
Deciduous Tree ◽  
Insecticide Treatment ◽  
Tissue Samples ◽  
Trunk Diameter ◽  
Herbicide Treatments ◽  
Nursery Production ◽  
Leaf Tissues ◽  
The Impact ◽  
Damage Protection

Abstract The flatheaded appletree borer, Chrysobothris femorata (Olivier) (Coleoptera: Buprestidae), and related species are deciduous tree pests. Female beetles prefer to oviposit at tree bases, and larvae tunnel beneath the bark, which weakens or kills young or newly transplanted trees. In the first objective of this study, Discus N/G (2.94% imidacloprid + 0.7% cyfluthrin) applied at six lower-than-labeled rates (0.0, 0.98, 1.97, 3.94, 5.91, and 7.87 ml/cm of average trunk dia.) was evaluated for protection of field-grown maples. A second objective evaluated imidacloprid with and without herbicides to assess the impact of weed competition at the tree base on insecticide effectiveness. A third objective determined relative imidacloprid concentrations in leaf tissue samples with ELISA and related to insecticide rates, herbicide treatments, and the level of flatheaded borer protection. In two trials, higher rates of insecticide were more effective at protecting trees, with rates ≥3.94 ml product/cm trunk diameter performing equivalently. Weed-free trees had more borer attacks and grew faster than trees in weedy plots. Imidacloprid content in leaf tissues had a trend for higher concentrations in smaller, weedy trees in the first season, but that pattern disappeared in subsequent years. Based on fewer attacks in weedy versus weed-free trees (60−90% reduction), it was concluded that weed presence can reduce borer attack success in nurseries independent of insecticide treatment, but tree growth was reduced by weed presence. In addition, Discus applied at rates >3.94 ml/cm did not confer added borer damage protection in weedy plots.

scholarly journals Pertumbuhan dan Produktivitas Daun Lamun Thalassia hemprichii (Ehrenb) Ascherson di Perairan Tanjung Benoa, Bali

2017 ◽  
Vol 4 (2) ◽  
pp. 278
Author(s):  
Budi Santoso ◽  
I Gusti Bagus Sila Dharma ◽  
Elok Faiqoh
Keyword(s):  
Growth Rate ◽  
Leaf Age ◽  
Leaf Tissue ◽  
Leaf Size ◽  
Dry Weight ◽  
Nutrient Content ◽  
Leaf Biomass ◽  
Thalassia Hemprichii ◽  
Average Growth ◽  
Young Leaves

Thalassia hemprichii is one of the seagrass as favorite food of two protected herbivores such as Green Turtle and Dugong. The existence of Thalassia hemprichii and knowing the growth rate becomes very important for sustainability of two herbivores. The study was conducted in Tanjung Benoa waters that covering three parts of the coast such as Samuh, Conrad and Tanjung beach in February until March 2017. Thalassia hemprichii leaves growth is observed consist of long and heavy growth (dry weight) and measured leaf age that has been observed such as young, half old and old leaves. The method that used to measure the growth of Thalassia hemprichii is pruning method and determination the research location using purposive sampling method. The aim of this research is explained the growth rate, biomass, and productivity of young leaves, old leaves of Thalassia hemprichii in Tanjung Benoa Bali. Measurement results of Thalassia hemprichii growth between age group of leaves has different young leaves faster growth if compared with the half old leaves and old leaves with average growth is 3.41mm/day indicates that the efficiency of this seagrass growth will decrease as the leaves age. The highest biomass values ??were found in older leaves if compared with young leaves and half-old leaves with average 0.205 gbk/m2 expected because the thick and wide leaf size will produced larger leaf biomass. The highest productivity value is found in oldest leaves in each station with average 0.028 gbk/m2/day expected morphology and different nutrient content in each leaf tissue.

Stem Cold Hardiness, Leaf Heat Tolerance, Growth, and Performance of Six Japanese Maples (Acer palmatum) in Northern Illinois

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 512e-512
Author(s):  
A.M. Shirazi
Keyword(s):  
Water Content ◽  
Heat Tolerance ◽  
Cold Hardiness ◽  
Block Design ◽  
Leaf Tissue ◽  
Ion Leakage ◽  
Randomized Block Design ◽  
Leaf Tissues ◽  
Artificial Freezing ◽  
And Performance

Six different Japanese Maples (Acer palmatum) cultivars `Water Fall', `Burgundy Lace', `Crimson Queen', `Oshio-Beni', `SangoKaKu', and `Bloodgood' from Monrovia Nursery were planted in a randomized block design on 4 June 1997 at the The Morton Arboretum. Leaf heat tolerance was evaluated by measuring ion leakage of the leaf tissue at 25–60 °C in July, Aug., and Sept. 1997. The LT50 (the temperature at which 50% of the tissues were injured) of all the cultivars were higher in July (≈53 °C) and were lower in September (≈47 °C). Water content of the leaf tissues were higher in July compare to August and September and were not related to heat tolerance of most cultivars. Stem cold hardiness was performed by artificial freezing tests in Oct., Dec., and Feb. 1997/98. The Lowest Survival Temperature (LST) for the most hardy to least hardy cultivars in October and December were: `Burgundy Lace' (–15, –27 °C), `Bloodgood' (–18, –24 °C), `Oshio-Beni' (–15, –24 °C), `Crimson Queen' (–15, –18 °C), `Water Fall' (–9, –18 °C) and `SangoKaKu' (–9, –12 °C), respectively. Growth, dormancy development, spring budbreak and performance of these cultivars will be compared.

scholarly journals Evidence that a New Antibiotic Flavone Glycoside Chemically Defends the Sea Grass Thalassia testudinumagainst Zoosporic Fungi

1998 ◽  
Vol 64 (4) ◽  
pp. 1490-1496 ◽  
Author(s):  
P. R. Jensen ◽  
K. M. Jenkins ◽  
D. Porter ◽  
W. Fenical
Keyword(s):  
Chemical Characterization ◽  
Leaf Tissue ◽  
Chemical Fractionation ◽  
Tissue Concentrations ◽  
Flavone Glycoside ◽  
Sea Grass ◽  
Zoosporic Fungi ◽  
Leaf Tissues ◽  
Marine Angiosperm

ABSTRACT Significantly fewer thraustochytrid protists (zoosporic fungi) were observed in association with healthy leaf tissue of the marine angiosperm Thalassia testudinum than in association with sterilized samples that were returned to the collection site for 48 h. In support of the hypothesis that sea grass secondary metabolites were responsible for these differences, extracts of healthyT. testudinum leaf tissues inhibited the growth of the co-occurring thraustochytrid Schizochytrium aggregatum and deterred the attachment of S. aggregatum motile zoospores to an extract-impregnated substrate. By using S. aggregatumfor bioassay-guided chemical fractionation, a new flavone glycoside was isolated and structurally characterized as luteolin 7-O-β-d-glucopyranosyl-2"-sulfate. Whole-leaf tissue concentrations of this metabolite (4 mg/ml of wet leaf tissue) inhibited S. aggregatum attachment, and a significantly lower concentration (270 μg/ml) reduced thraustochytrid growth by 50%, suggesting that natural concentrations are at least 15 times greater than that needed for significant microbiological effects. These results offer the first complete chemical characterization of a sea grass sulfated flavone glycoside and provide evidence that a secondary metabolite chemically defends T. testudinum against fouling microorganisms.

scholarly journals VEGETATIVE GROWTH AND NUTRIENT LEVELS OF LINGONBERRIES GROWN IN FOUR ALASKAN SUBSTRATES

1982 ◽  
Vol 62 (4) ◽  
pp. 969-977 ◽  
Author(s):  
PATRICIA S. HOLLOWAY ◽  
ROBERT M. VAN VELDHUIZEN ◽  
CECIL STUSHNOFF ◽  
DAVID K. WILDUNG
Keyword(s):  
Vegetative Growth ◽  
Sandy Loam ◽  
Leaf Size ◽  
Maximum Growth ◽  
Stem Length ◽  
Dry Matter Accumulation ◽  
Silt Loam ◽  
Tissue Samples ◽  
Silt Loam Soil ◽  
Loam Soil

Vegetative growth of lingonberries was observed on plants growing in four unsterilized, native-Alaskan substrates: coarsely-ground Lemeta peat, Fairbanks silt loam soil, a mixture of peat and silt loam soil and washed Chena very fine sandy loam soil. Following three growing seasons, plants in the peat treatment showed the greatest increase in vegetative growth as revealed by the number of new stems produced, stem length and dry weight per plant. Leaf size did not differ among substrate treatments. The leaves on plants grown in the peat substrate remained green throughout the entire experiment. The leaves of plants in all other treatments showed varying degrees of chlorosis followed by reddening and necrosis. Differences in concentration of N, P, K, Mn, Fe, Zn and Al in whole-plant tissue samples were recorded. The results indicate lingonberries should be grown in a peat substrate for maximum growth and dry matter accumulation.

scholarly journals To the question of the origin of the cultural assortment   of Dagestan apricots through assessing the variability   of leaf morphological characteristics

2019 ◽  
Vol 14 (3) ◽  
pp. 17-24
Author(s):  
D. M. Anatov ◽  
Z. M. Аsadulaev ◽  
R. M. Osmanov ◽  
K. I. Akhmedova
Keyword(s):  
Leaf Shape ◽  
Morphological Characteristics ◽  
Principal Component ◽  
Leaf Size ◽  
Shape Index ◽  
Botanical Garden ◽  
Mahalanobis Distances ◽  
Traditional Cultivars ◽  
Academy Of Sciences ◽  
Degree Of Similarity

Aim. The paper presents the results of assessment of the indigenous nature and  degree of similarity of apricot cultivars growing in the collection of the Mountain  Botanical Garden, Gunib, Dagestan, Russia based on a comparative analysis of the  variability of leaf morphological characteristics.   Material and Methods. The material assessed consisted of 33 apricot cultivars of  various ecological and geographical origins aggregated in the following groups: (a)  Dagestan – traditional cultivars; (b) Moscow ‐ selection from the Tsytsin Main Moscow Botanical Garden, Russian Academy of Sciences based on wild forms of Tajikistan and Kyrgyzstan; (c) European and (d) Asian ‐ from Central Asia, Tajikistan, China  and Altai.   Results. The closeness of Dagestan and European varieties in comparison with Asian  and Moscow varieties was shown. Most Dagestan (16 of 19) and European varieties  have round‐shaped leaves (leaf shape index 80‐ 100%), while those from Asia and  the Moscow Botanical Garden have leaves which are elongated elliptical and oval  (60‐80%). Using the method of principal component analysis (PCA), it was established that most cultivars of Dagestan origin have similar leaf shapes and sizes, of  which Tlama kurak (wide‐round), Hekobarsh (elongated) were distinguished by leaf  shape and Esdelik by leaf size.   Conclusion. Based on a discriminant analysis (Squared Mahalanobis Distances), it  was found that the indices of indicators of leaf attributes (width/length of leaf lamina; petiole length/length of lamina; apex angle/corner of leaf base) are more reliable criteria for differentiating apricot varieties into ecological and geographical  groups than their morphological characteristics.  

scholarly journals First report of Coleus blumei viroid 1 in commercial Coleus blumei in the United States

Plant Disease ◽  
2021 ◽  
Author(s):  
Gardenia Orellana ◽  
Alexander V Karasev
Keyword(s):  
United States ◽  
Leaf Tissue ◽  
The United States ◽  
Universal Primers ◽  
Rt Pcr ◽  
Universal Primer ◽  
Tissue Samples ◽  
First Report ◽  
Specific Pcr ◽  
Coleus Blumei

Coleus scutellarioides (syn. Coleus blumei) is a widely grown evergreen ornamental plant valued for its highly decorative variegated leaves. Six viroids, named Coleus blumei viroid 1 to 6 (CbVd-1 to -6) have been identified in coleus plants in many countries of the world (Nie and Singh 2017), including Canada (Smith et al. 2018). However there have been no reports of Coleus blumei viroids occurring in the U.S.A. (Nie and Singh 2017). In April 2021, leaf tissue samples from 27 cultivars of C. blumei, one plant of each, were submitted to the University of Idaho laboratory from a commercial nursery located in Oregon to screen for the presence of viroids. The sampled plants were selected randomly and no symptoms were apparent in any of the samples. Total nucleic acids were extracted from each sample (Dellaporta et al. 1983) and used in reverse-transcription (RT)-PCR tests (Jiang et al. 2011) for the CbVd-1 and CbVd-5 with the universal primer pair CbVds-P1/P2, which amplifies the complete genome of all members in the genus Coleviroid (Jiang et al. 2011), and two additional primer pairs, CbVd1-F1/R1 and CbVd5-F1/R1, specific for CbVd-1 and CbVd-5, respectively (Smith et al. 2018). Five C. blumei plants (cvs Fire Mountain, Lovebird, Smokey Rose, Marrakesh, and Nutmeg) were positive for a coleviroid based on the observation of the single 250-nt band in the RT-PCR test with CbVds-P1/P2 primers. Two of these CbVd-1 positive plants (cvs Lovebird and Nutmeg) were also positive for CbVd-1 based on the presence of a single 150-nt band in the RT-PCR assay with CbVd1-F1/R1 primers. One plant (cv Jigsaw) was positive for CbVd-1, i.e. showing the 150-nt band in RT-PCR with CbVd1-F1/R1 primers, but did not show the ca. 250-bp band in RT-PCR with primers CbVds-P1/P2. None of the tested plants were positive for CbVd-5, either with the specific, or universal primers. All coleviroid- and CbVd-1-specific PCR products were sequenced directly using the Sanger methodology, and revealed whole genomes for five isolates of CbVd-1 from Oregon, U.S.A. The genomes of the five CbVd-1 isolates displayed 96.9-100% identity among each other and 96.0-100% identity to the CbVd-1 sequences available in GenBank. Because the sequences from cvs Lovebird, Marrakesh, and Nutmeg, were found 100% identical, one sequence was deposited in GenBank (MZ326145). Two other sequences, from cvs Fire Mountain and Smokey Rose, were deposited in the GenBank under accession numbers MZ326144 and MZ326146, respectively. To the best of our knowledge, this is the first report of CbVd-1 in the United States.

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