scholarly journals Potential nutrient-response curves and sufficiency ranges of ‘Grande Naine’ banana cultivated in two environment

2021 ◽  
Vol 43 (1) ◽  
Author(s):  
Vagner Alves Rodrigues Filho ◽  
Júlio César Lima Neves ◽  
Sérgio Luiz Rodrigues Donato ◽  
Bruno Vinícius Castro Guimarães
Keyword(s):  
Nutritional Status ◽  
Relative Yield ◽  
Leaf Tissue ◽  
Reference Population ◽  
Nutrient Concentrations ◽  
Boundary Line ◽  
Response Curves ◽  
Data Set ◽  
Tissue Samples ◽  
Nutrient Response

Abstract Adequate plant nutrition is essential to attain higher yields. The objective was to determine potential-nutrient response curves and sufficiency ranges using the boundary line approach and balance indices of Kenworthy for interpreting the nutritional status of ‘Grand Nain’ banana cultivated in two environments. The study was carried out using a database containing leaf nutrient concentrations and yields of bananas cultivated on two areas located in Missão Velha-CE, and Ponto Novo-BA, Brazil. Plots with high-yielding plants, which were those with yields above average plus 0.5 standard deviation, were used atem as reference population. The database was subdivided into two sets. One of them contained 46 leaf tissue samples and reference population with yield greater than 58.84 Mg ha-1 year-1, in Missão Velha-CE. The second data set contained 19 samples and reference population with yield greater than 76.12 Mg ha-1 year-1 in Ponto Novo-BA. Potential response curves were fitted to the relationship between relative yield and leaf element concentrations and balance indices of Kenworthy. Models expressed high predictive power. Sufficiency ranges for macro- and micronutrient concentrations and balance indices of Kenworthy were established. The ranges allow an improved nutritional status assessment of irrigated ‘Grand Nain’ bananas.

scholarly journals Reference values and diagnostic ranges to assess the degree of nutritional balance for cacao plants

2021 ◽  
Vol 19 (1) ◽  
pp. e0801
Author(s):  
Bismark L. Bahia ◽  
José O. Souza-Júnior ◽  
Loane V. Fernandes ◽  
Júlio C. L. Neves
Keyword(s):  
Relative Yield ◽  
Agroforestry Systems ◽  
Boundary Line ◽  
Potential Response ◽  
Response Curves ◽  
Cultivation System ◽  
Cultivation Systems ◽  
Leaf Analysis ◽  
General Norm ◽  
Nutritional Balance

Aim of study: The interpretation of results of leaf analysis can be performed by nutritional balance methods, such as Kenworthy method (KW) and diagnostic levels of contents, whose achievements for cacao constitute the main objective of this work.Area of study: Bahia, Brazil.Material and methods: The database covered cacao trees in two cultivation systems: agroforestry systems and full sun. The reference populations were composed of plots with relative yield higher than the average plus half a standard deviation of each of these cultivation systems, in addition to a combined population of both systems.Main results: The norms of the KW method were compared by the t test, for mean, with 72% concordance; and F, for variance, 82% concordant. The diagnoses made based on specific norms per cultivation system and the general norm agreed on average of 91%. Potential response curves were obtained as a function of the Balanced Indices of Kenworthy (BIK) for each nutrient, by the boundary-line method, in addition to sufficiency ranges for BIK and for leaf contents for cacao.Research highlights: It is concluded that the general KW norms associated with the original Kenworthy ranges or the specific ranges for cacao are efficient in the nutritional diagnosis of cacao.

EFFECT OF METHOD AND DATE OF PRUNING ON SOIL ORGANIC MATTER AND LEAF NUTRIENT CONCENTRATIONS OF LOWBUSH BLUEBERRIES

1982 ◽  
Vol 62 (3) ◽  
pp. 813-817 ◽  
Author(s):  
ERIC J. HANSON ◽  
AMR. A. ISMAIL ◽  
ROLAND A. STRUCHTEMEYER
Keyword(s):  
Organic Matter ◽  
Soil Organic Matter ◽  
Leaf Tissue ◽  
Growing Season ◽  
Nutrient Concentrations ◽  
Vaccinium Angustifolium ◽  
Tissue Samples ◽  
Lowbush Blueberry ◽  
Leaf Nutrient Concentrations

Lowbush blueberry (Vaccinium angustifolium Ait.) plants were pruned mechanically by flail mowing, or thermally by oil burning in the spring or fall. The pruning treatments had no effect on soil organic matter or pH. Leaf tissue samples taken from burned plants the first growing season after pruning were higher in N and P, but lower in Ca than leaf samples from mowed plants. Leaf tissue samples taken the second growing season following pruning were not influenced by treatments.

scholarly journals Nutrient sufficiency levels for haskap (Lonicera caeruleaL.) using the boundary-line approach

2019 ◽  
Vol 99 (2) ◽  
pp. 268-280 ◽  
Author(s):  
Ekene Mark-Anthony Iheshiulo ◽  
Lord Abbey ◽  
Andrew M. Hammermeister
Keyword(s):  
Growth Rate ◽  
Nova Scotia ◽  
Leaf Tissue ◽  
Principal Component ◽  
Leaf Size ◽  
Nutrient Status ◽  
Boundary Line ◽  
Tissue Samples ◽  
Leaf Tissues ◽  
Representative Farms

Adequate supply of plant nutrients is crucial for haskap plant growth and increased productivity. A study was carried out to determine the variability in haskap (Lonicera caerulea L. cv. Indigo Gem) plant characteristics in relation to soil and leaf tissue nutrient status. A total of 19 composite soil samples and corresponding plant leaf tissue samples were collected in 2016 from 12 locations in Nova Scotia. Plant parameters measured include growth rate, leaf size, leaf chlorophyll content, and visual observations. A boundary-line approach was used to determine nutrient sufficiency ranges in leaf tissues of 2.23%–2.96% for N, 0.22%–0.28% for P, 0.84%–1.32% for K, 1.63%–2.10% for Ca, and 0.14%–0.50% for Mg. Principal component and correlation analysis suggested a possible antagonistic interaction between leaf K and Mg. Negative associations were observed most frequently between Ca and Mg and other nutrients, especially K. Plant parameters such as bush volume, leaf size, and growth rate were closely related to soil and leaf K. Deficiencies in leaf tissue K and P were identified as potentially important factors limiting growth. Therefore, there is a need to adjust or balance the application of these nutrients. In conclusion, the sufficiency ranges derived can be used as a guiding principle in diagnosing the nutritional status of haskap cv. Indigo Gem on representative farms in Nova Scotia.

scholarly journals Beaufils ranges to assess the cotton nutrient status in the southern region of Mato Grosso

2012 ◽  
Vol 36 (1) ◽  
pp. 171-182 ◽  
Author(s):  
Ademar Pereira Serra ◽  
Marlene Estevão Marchetti ◽  
Enrique Pouyú Rojas ◽  
Antônio Carlos Tadeu Vitorino
Keyword(s):  
Nutritional Status ◽  
Nutrient Status ◽  
Reference Population ◽  
Integrated System ◽  
Nutrient Concentrations ◽  
Yield Data ◽  
Mato Grosso ◽  
Nutrient Contents ◽  
Foliar Concentrations ◽  
The Relationship

The relationships between nutrient contents and indices of the Diagnosis and Recommendation Integrated System (DRIS) are a useful basis to determine appropriate ranges for the interpretation of leaf nutrient contents. The purpose of this study was to establish Beaufils ranges from statistical models of the relationship between foliar concentrations and DRIS indices, generated by two systems of DRIS norms - the F value and natural logarithm transformation - and assess the nutritional status of cotton plants, based on these Beaufils ranges. Yield data from plots (average acreage 100 ha) and foliar concentrations of macro and micronutrients of cotton (Gossypium hirsutum r. latifolium) plants, in the growing season 2004/2005, were stored in a database. The criterion to define the reference population consisted of plots with above-average yields + 0.5 standard deviation (over 4,575 kg ha-1 seed cotton yield). The best-fitting statistical model of the relationship between foliar nutrient concentrations and DRIS indices was linear, with R² > 0.8090, p < 0.01, except for N, with R² = 0.5987, p < 0.01. The two criteria were effective to diagnose the plant nutritional status. The diagnoses were not random, but based on the effectiveness of the chi-square-tested method. The agreement between the methods to assess the nutritional status was 92.59-100 %, except for S, with 74.07 % agreement.

scholarly journals EFFECT OF POULTRY LITTER BIOCHAR ON THE NUTRITIONAL STATUS OF CORN1

2021 ◽  
Vol 34 (4) ◽  
pp. 916-925
Author(s):  
JACQUELINE DA SILVA MENDES ◽  
JOSELY DANTAS FERNANDES ◽  
LÚCIA HELENA GARÓFALO CHAVES ◽  
GILVANISE ALVES TITO ◽  
HUGO ORLANDO CARVALLO GUERRA
Keyword(s):  
Nutritional Status ◽  
Poultry Litter ◽  
Leaf Tissue ◽  
Nutrient Concentrations ◽  
Randomized Design ◽  
Opposite Leaf ◽  
Poultry Litter Biochar ◽  
Completely Randomized Design ◽  
Forced Air ◽  
Air Circulation

ABSTRACT Corn, one of the main grain crops in Brazil, needs to have its nutritional requirements fully satisfied to achieve high biological productivity. Thus, the objective of this work was to evaluate the influence of increasing doses of poultry litter biochar on nutrient concentrations in the leaves of hybrid corn BRS 2022 and in the soil after harvest. The experiment was carried out in a completely randomized design, with four replicates, evaluating six doses of biochar (0; 2.02; 4.05; 6.07; 8.10 and 10.12 t ha-1) and the plots composed of one plant per pot with a volume of 20 dm3. The collection of leaves for leaf diagnosis was carried out at the time of flowering, removing the opposite leaf from the ear base in the middle third region. These leaves were dried in a forced air circulation oven, 65 °C, for a period of 48 hours, ground, sieved through 20 mesh and analyzed for the concentrations of macronutrients in the leaf tissue. At the end of the experiment, 83 days after corn sowing, soil samples were collected as a function of the treatments and then analyzed chemically. Biochar application promoted an increase in the leaf contents of N, P and K, resulting in improvements in the nutritional status of plants for these nutrients. The chemical characteristics of the soil, analyzed after the corn harvest, revealed that there was an influence of the doses of biochar on the levels of calcium, organic carbon, potassium and phosphorus.

scholarly journals Establishment of Leaf Tissue Nutrient Sufficiency Ranges of Two Perovskia Selections by Chronological Age

HortScience ◽  
2020 ◽  
Vol 55 (8) ◽  
pp. 1303-1307
Author(s):  
W. Garrett Owen
Keyword(s):  
Tissue Concentration ◽  
Leaf Tissue ◽  
Growth Index ◽  
Water Soluble ◽  
Chronological Age ◽  
Nutrient Concentrations ◽  
Axillary Shoot ◽  
Tissue Samples ◽  
Total N ◽  
Shoot Number

The objective of this study was to determine optimum fertilizer concentrations, identify leaf tissue nutrient sufficiency ranges by chronological age, and establish leaf tissue nutrient standards of containerized Russian sage (Perovskia sp.). Common Russian sage (P. atriplicifolia Benth.) and ‘Crazy Blue’ Russian sage were greenhouse-grown in a soilless substrate under one of six constant liquid fertilizer concentrations [50, 75, 100, 200, 300, or 400 mg·L−1 nitrogen (N)] with a constant level of a water-soluble micronutrient blend. Fertilizer concentrations sufficient for optimal plant growth and development were determined by analyzing plant height, diameter, growth index, primary shoot caliper, axillary shoot number, and total dry mass; they were found to be 100 to 200 mg·L−1 N after a 6-week crop cycle. Recently, mature leaf tissue samples were collected from plants fertilized with 100 to 200 mg·L−1 N and analyzed for elemental contents of 11 nutrients at 2, 4, and 6 weeks after transplant (WAT). An overall trend of increasing foliar nutrient concentrations over time was observed for all elemental nutrients. For instance, at 2 WAT, the total N concentrations of common Russian sage and ‘Crazy Blue’ Russian sage ranged between 3.68% and 5.10% and between 3.92% and 5.12%, respectively, and increased to ranges of 5.94% to 5.98% and 5.20% to 5.86% at 6 WAT, respectively. Before this study, no leaf tissue concentration standards have been reported; therefore, this study established leaf tissue concentration sufficiency ranges for the trialed Perovskia selections.

Whole genome transcriptome analysis of the stomach resected in human vertical sleeve gastrectomy: cutting more than calories

2021 ◽  
Author(s):  
William C Dungan ◽  
Michael R Garrett ◽  
Bradley A. Welch ◽  
William J Lawson ◽  
Alexandra R Himel ◽  
...  
Keyword(s):  
Body Weight ◽  
Sleeve Gastrectomy ◽  
Transcriptome Analysis ◽  
Gene Signature ◽  
Whole Genome ◽  
Vertical Sleeve Gastrectomy ◽  
Data Set ◽  
Tissue Samples ◽  
Highly Expressed Genes ◽  
A Prospective Study

Background. Vertical sleeve gastrectomy (VSG) is a surgical weight loss procedure that resects 80% of the stomach, creating a tube linking the esophagus to the duodenum. Because of the efficacy and relative simplicity of VSG, it is preferred in the U.S with VSG currently at >61% of bariatric surgeries performed. Surprisingly, there has never been a complete molecular characterization of the human stomach fundus and corpus. Here we compare and contrast the molecular make-up of these regions. Methods. We performed a prospective study to obtain gastric tissue samples from patients undergoing VSG. Paired fundus and corpus samples were obtained Whole genome transcriptome analysis was performed by RNA sequencing, with key findings validated by qPCR. Results. Participants were primarily female (95.8%) and white (79.15%). Mean BMI, body weight, and age were 46.1 kg/m2, 121.6 kg, and 43.29 years, respectively. Overall, 432 gene transcripts were significantly different between the fundus and corpus (p<0.05). A significant correlation was found between the RNAseq data set and qPCR validation, demonstrating robust gene expression differences. Significant genes included: progastricsin, acid chitinase, gastokine 1 and 2 in both fundus and corpus. Of the very highly expressed genes in both regions, 87% were present in both the stomach's fundus and corpus, indicating substantial overlap. Conclusions. Despite significant overlap in the greater curvature gene signature, regional differences exist. Given that the mechanism of VSG is partly unresolved, the potential that the resected tissue may express genes that influence long-term body weight regulation is unknown and could influence VSG outcomes.

scholarly journals First report of Coleus blumei viroid 1 in commercial Coleus blumei in the United States

Plant Disease ◽  
2021 ◽  
Author(s):  
Gardenia Orellana ◽  
Alexander V Karasev
Keyword(s):  
United States ◽  
Leaf Tissue ◽  
The United States ◽  
Universal Primers ◽  
Rt Pcr ◽  
Universal Primer ◽  
Tissue Samples ◽  
First Report ◽  
Specific Pcr ◽  
Coleus Blumei

Coleus scutellarioides (syn. Coleus blumei) is a widely grown evergreen ornamental plant valued for its highly decorative variegated leaves. Six viroids, named Coleus blumei viroid 1 to 6 (CbVd-1 to -6) have been identified in coleus plants in many countries of the world (Nie and Singh 2017), including Canada (Smith et al. 2018). However there have been no reports of Coleus blumei viroids occurring in the U.S.A. (Nie and Singh 2017). In April 2021, leaf tissue samples from 27 cultivars of C. blumei, one plant of each, were submitted to the University of Idaho laboratory from a commercial nursery located in Oregon to screen for the presence of viroids. The sampled plants were selected randomly and no symptoms were apparent in any of the samples. Total nucleic acids were extracted from each sample (Dellaporta et al. 1983) and used in reverse-transcription (RT)-PCR tests (Jiang et al. 2011) for the CbVd-1 and CbVd-5 with the universal primer pair CbVds-P1/P2, which amplifies the complete genome of all members in the genus Coleviroid (Jiang et al. 2011), and two additional primer pairs, CbVd1-F1/R1 and CbVd5-F1/R1, specific for CbVd-1 and CbVd-5, respectively (Smith et al. 2018). Five C. blumei plants (cvs Fire Mountain, Lovebird, Smokey Rose, Marrakesh, and Nutmeg) were positive for a coleviroid based on the observation of the single 250-nt band in the RT-PCR test with CbVds-P1/P2 primers. Two of these CbVd-1 positive plants (cvs Lovebird and Nutmeg) were also positive for CbVd-1 based on the presence of a single 150-nt band in the RT-PCR assay with CbVd1-F1/R1 primers. One plant (cv Jigsaw) was positive for CbVd-1, i.e. showing the 150-nt band in RT-PCR with CbVd1-F1/R1 primers, but did not show the ca. 250-bp band in RT-PCR with primers CbVds-P1/P2. None of the tested plants were positive for CbVd-5, either with the specific, or universal primers. All coleviroid- and CbVd-1-specific PCR products were sequenced directly using the Sanger methodology, and revealed whole genomes for five isolates of CbVd-1 from Oregon, U.S.A. The genomes of the five CbVd-1 isolates displayed 96.9-100% identity among each other and 96.0-100% identity to the CbVd-1 sequences available in GenBank. Because the sequences from cvs Lovebird, Marrakesh, and Nutmeg, were found 100% identical, one sequence was deposited in GenBank (MZ326145). Two other sequences, from cvs Fire Mountain and Smokey Rose, were deposited in the GenBank under accession numbers MZ326144 and MZ326146, respectively. To the best of our knowledge, this is the first report of CbVd-1 in the United States.

Enhanced production of falcarinol-type polyacetylenes in hairy roots of cultivated carrot (Daucus carota subsp. sativus)

2021 ◽  
Author(s):  
Frank Dunemann ◽  
Christoph Böttcher
Keyword(s):  
Hairy Root ◽  
Large Scale ◽  
Higher Plants ◽  
Leaf Tissue ◽  
Hairy Root Cultures ◽  
Scale Production ◽  
Root Cultures ◽  
Tissue Samples ◽  
Positive Effects ◽  
Enhanced Production

Abstract Polyacetylenes (PAs) are a large group of bioactive phytochemicals, which are primarily produced by higher plants of the families Apiaceae and Araliaceae. Especially aliphatic C17-polyacetylenes of the falcarinol-type such as falcarinol (FaOH) and falcarindiol (FaDOH) are known for their numerous positive effects on human health. In this study we investigate the potential of carrot hairy root cultures for production of PAs. Three individual plants of seven differently coloured carrot cultivars were used for the development of hairy root cultures by transformation of root discs with the wild-type Rhizobium rhizogenes strain 15834. A total of 51 individual hairy root (HR) lines were obtained and quantitatively analysed together with root, petiole and leaf tissue samples for FaOH and FaDOH. Among the five tissues sampled from the donor plants, root periderm samples generally exhibited the highest PA levels with FaDOH as prevailing PA and large differences between cultivars. In comparison to periderm tissue, FaOH levels were highly increased in HR lines of all cultivars. In contrast, FaDOH levels were not significantly altered. Considering the low to moderate PA concentration in root and leaf tissues of the orange cultivars there was an up to more than 10-fold increase of the FaOH concentration in HRs of these genotypes. Within this study a reproducible method for Rrhi-mediated transformation of carrot root discs was applied which provides an efficient tool to assess the function of candidate genes involved in the biosynthesis of key PAs in carrot but might be used in future also for the large-scale production of falcarinol-type PAs.

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