scholarly journals The growth of Paracoccus halodenitrificans in a defined medium

1984 ◽  
Vol 30 (6) ◽  
pp. 837-840 ◽  
Author(s):  
Lawrence I. Hochstein ◽  
Geraldine A. Tomlinson
Keyword(s):  
Synthetic Medium ◽  
Carbon Sources ◽  
Defined Medium ◽  
Anaerobic Growth ◽  
Inorganic Salts ◽  
Vitamin B ◽  
Aerobic Growth ◽  
Methionine Biosynthesis ◽  
Dependent Pathway

A synthetic medium, consisting of inorganic salts and any of a number of carbon sources, supported the aerobic growth of Paracoccus halodenitrificans when supplemented with thiamine. The same medium plus an appropriate nitrogenous oxide supported anaerobic growth when additionally supplemented with methionine. The observation that vitamin B12 or betaine replaced methionine suggested that P. halodenitrificans had a defect in the cobalamin-dependent pathway for methionine biosynthesis, as well as the inability to synthesize betaine when growing anaerobically.

scholarly journals PSII-7 Complete genome analysis of a novel mucolytic bacterium, Prevotella mucinisolvens sp. nov., isolated from bovine rumen epithelium

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 406-406
Author(s):  
Soo Jong Hong ◽  
Do Hyun Kim ◽  
Da Jin Sol Jung ◽  
Md Najmul Haque ◽  
Myunggi Baik
Keyword(s):  
Complete Genome ◽  
Messenger Rna ◽  
Carbon Sources ◽  
Extracellular Polysaccharide ◽  
Defined Medium ◽  
Glycoside Hydrolases ◽  
Stress Reactions ◽  
Illumina Hiseq ◽  
Rumen Epithelium ◽  
Dependent Pathway

Abstract We report the complete genome sequence of a novel mucolytic bacterium, Prevotella mucinisolvens sp. nov. Mucolytic bacteria were isolated from rumen epithelium of the dorsal sac of Korean cattle steer using a targeted cultivation on a mucin defined medium as a sole carbon source in anaerobic conditions. The genome of P. mucinisolvens was sequenced by means of both the Illumina HiSeq™ X and PacBio RSII platforms. The genome (2,730,135 bp) was found to contain 2,445 genes, 2,374 coding sequences, 61 transfer RNA, 1 transfer-messenger RNA, and 9 ribosomal RNA. The P. mucinisolvens had a total 51 glycoside hydrolases (GHs), of which 14 GHs, including β-galactosidases (GH2, GH20), α-N-acetylgalactosaminidases (GH101), α-N-acetylglucosaminidase (GH89), sialidase (GH33), and fucosidases (GH29, GH95), were identified as enzymes involved in mucin degradation. Following the KEGG pathways, the putative mucolytic pathway was constructed, including the metabolism of carbon sources such as galactose, N-acetylglucosamine, sialic acid (N-acetylneuraminic acid), and mannose. The presence of putative extracellular polysaccharide biosynthesis pathways, including Wzx/Wzy-dependent pathway (4 putative glycosyltransferases, 3 acetyltransferases, 1 flippase, 1 polymerase, 1 polysaccharide co-polymerase, and 1 outer membrane transport protein) and synthase-dependent pathway (1 putative synthase, 3 precursors of synthesis), was confirmed in P. mucinisolvens. Twelve putative virulence factors associated with adherence (hasB, KpsF, and htpB), stress reactions (clpP and clpC), antiphagocytosis (hasB and bcs1), O-antigen (gmd, fcl, and galE), and metabolic adaption (panD) were identified. This study contributes to a better understanding of epimural bacteria in putative mucin-degrading ability.

A CHEMICALLY DEFINED MEDIUM FOR HALOBACTERIUM SALINARIUM STRAIN 1

1963 ◽  
Vol 9 (4) ◽  
pp. 619-624 ◽  
Author(s):  
Ian D. Dundas ◽  
V. R. Srinivasan ◽  
H. Orin Halvorson
Keyword(s):  
Amino Acids ◽  
Growth Rates ◽  
Synthetic Medium ◽  
Defined Medium ◽  
Inorganic Salts ◽  
Chemically Defined Medium ◽  
Halobacterium Salinarium ◽  
Complex Media ◽  
Isoleucine Leucine

A chemically defined medium has been composed for Halobacterium salinarium strain 1. The medium consists of inorganic salts, 10 amino acids (lysine, arginine, proline, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, and glutamine) and cytidylic acid. The amino acids valine, methionine, isoleucine, and leucine are found to be essential for growth in this medium. Growth rates in the synthetic medium are not as high as those obtained in complex media. The medium allows growth of several halophilic organisms.

A STUDY OF THE NUTRITIONAL REQUIREMENTS AND TOXIN PRODUCTION OF CLOSTRIDIUM BOTULINUM TYPE F

1965 ◽  
Vol 11 (6) ◽  
pp. 1009-1019 ◽  
Author(s):  
Lillian V. Holdeman ◽  
Louis Ds. Smith
Keyword(s):  
Amino Acids ◽  
Clostridium Botulinum ◽  
Synthetic Medium ◽  
Toxin Production ◽  
Defined Medium ◽  
Inorganic Salts ◽  
Nutritional Requirements ◽  
Chemically Defined Medium ◽  
Aminobenzoic Acid ◽  
Botulinum Type

Clostridium botulinum type F was grown in a chemically defined medium containing 17 amino acids, 11 vitamins, glucose, and inorganic salts. The nutritional requirements were determined using single-omission test media. Arginine, tryptophan, tyrosine, valine, biotin, thiamin, and possibly methionine were essential nutrients. Growth was stimulated by glycine, isoleucine, phenylalanine, and para-aminobenzoic acid. Toxin was present in supernatant fluids from all synthetic medium cultures in which there was marked growth. In general, toxicity of synthetic medium cultures was about 1/10 that of complex medium cultures.Toxin and precursor appear to be formed intracellularly, for both were released by rupturing young cells with sonic vibration. Protoxin could be activated by treatment with trypsin.

scholarly journals The enzymes of B. coli communis . Part V.—(a) anaerobic growth followed by anaerobic and aerobic fermentation. (b) the effects of aeration during the fermentation

1921 ◽  
Vol 92 (644) ◽  
pp. 135-150
Keyword(s):  
Anaerobic Growth ◽  
Aerobic Growth ◽  
Aerobic Fermentation ◽  
The Subject ◽  
The Difference ◽  
Effect Of Oxygen ◽  
Present Section ◽  
Ferment Glucose

In the present communications the effect of oxygen upon the fermentation of glucose and upon the growth of the bacteria, in so far as this affects fermentation, is considered. To this end the organisms have been grown both aerobically and anaerobically, and subsequently made to ferment glucose, both aerobically and anaerobically, with the object of comparing the products of decomposition in the two cases. There are clearly two problems : firstly, the effect of exposure to oxygen during growth upon the subsequent fermentation, whether aerobic or anaerobic, and, secondly, the effect of oxygen admitted during the fermentation. The first question relates to the part played by oxygen in the formation of enzymes, the second to the part played by oxygen in their action on carbohydrates. The first question is considered, though in but a preliminary way, in Section A, the second, more fully, in Section B. Section A. Object of the Experiments . Two results were aimed at in these experiments. Firstly, to compare the products of fermentation of glucose anaerobically, after anaerobic growth, with the products of fermentation anaerobically after previous growth aerobically. And, secondly, to obtain information as to the effect of introducing oxygen during the fermentation itself. This latter consideration, however, though brought to notice by these experiments, is considered only incidentally here because it forms the subject of Section B. In the present section we wish to direct attention particularly to those differences which exist between the fermentation after anaerobic and aerobic growth, not upon the effect of aeration during the fermentation. To point out the difference which previous growth aerobically or anaerobically has made, several analyses from previous experiments are included in Table IV side by side with the completely anaerobic experiments of Tables I, II, and III.

scholarly journals Conditional Essentiality of the csrA Gene in Escherichia coli

2008 ◽  
Vol 191 (5) ◽  
pp. 1722-1724 ◽  
Author(s):  
Johan Timmermans ◽  
Laurence Van Melderen
Keyword(s):  
Escherichia Coli ◽  
Growth Inhibition ◽  
Deletion Mutant ◽  
Glycogen Synthesis ◽  
Synthetic Medium ◽  
Carbon Sources ◽  
Krebs Cycle ◽  
Physiological Processes

ABSTRACT CsrA is a global posttranscriptional regulator of numerous physiological processes, such as glycogenesis and glycolysis. Here, we show that the csrA gene of Escherichia coli is essential for growth on LB and on synthetic medium containing glycolytic carbon sources. However, csrA is not necessary for growth on synthetic medium containing pyruvate, showing that the Krebs cycle is functional in the csrA::cat deletion mutant. Deletion of the glgCAP operon in the csrA::cat mutant restored the ability to grow on LB and on synthetic medium containing glycolytic carbon sources, showing that growth inhibition is due to an excess of glycogen synthesis.

scholarly journals Interrelations between Glycine Betaine Catabolism and Methionine Biosynthesis in Sinorhizobium meliloti Strain 102F34

2006 ◽  
Vol 188 (20) ◽  
pp. 7195-7204 ◽  
Author(s):  
Lise Barra ◽  
Catherine Fontenelle ◽  
Gwennola Ermel ◽  
Annie Trautwetter ◽  
Graham C. Walker ◽  
...  
Keyword(s):  
Glycine Betaine ◽  
Sinorhizobium Meliloti ◽  
Wild Type Strain ◽  
Methionine Synthase ◽  
Vitamin B ◽  
Wild Type ◽  
Methionine Biosynthesis ◽  
Catabolic Pathway ◽  
Methyl Transferase ◽  
Meliloti Strain

ABSTRACT Methionine is produced by methylation of homocysteine. Sinorhizobium meliloti 102F34 possesses only one methionine synthase, which catalyzes the transfer of a methyl group from methyl tetrahydrofolate to homocysteine. This vitamin B12-dependent enzyme is encoded by the metH gene. Glycine betaine can also serve as an alternative methyl donor for homocysteine. This reaction is catalyzed by betaine-homocysteine methyl transferase (BHMT), an enzyme that has been characterized in humans and rats. An S. meliloti gene whose product is related to the human BHMT enzyme has been identified and named bmt. This enzyme is closely related to mammalian BHMTs but has no homology with previously described bacterial betaine methyl transferases. Glycine betaine inhibits the growth of an S. meliloti bmt mutant in low- and high-osmotic strength media, an effect that correlates with a decrease in the catabolism of glycine betaine. This inhibition was not observed with other betaines, like homobetaine, dimethylsulfoniopropionate, and trigonelline. The addition of methionine to the growth medium allowed a bmt mutant to recover growth despite the presence of glycine betaine. Methionine also stimulated glycine betaine catabolism in a bmt strain, suggesting the existence of another catabolic pathway. Inactivation of metH or bmt did not affect the nodulation efficiency of the mutants in the 102F34 strain background. Nevertheless, a metH strain was severely defective in competing with the wild-type strain in a coinoculation experiment.

FAT PRODUCTION FROM CANE MOLASSES BY PENICILLIUM SPINULOSUM: A STUDY ON SUBSTANCES IN CANE MOLASSES INTERFERING WITH FAT PRODUCTION

1961 ◽  
Vol 7 (6) ◽  
pp. 895-899 ◽  
Author(s):  
A. W. Khan ◽  
T. K. Walker
Keyword(s):  
Defined Medium ◽  
Fat Content ◽  
Inorganic Materials ◽  
Inorganic Salts ◽  
Cane Molasses

Production of fat by Penicillium spinulosum in cane molasses was poorer than in a defined medium containing sucrose and inorganic salts. The fat content of dry felt obtained in defined medium was 63.8% and the fat coefficient 16.1. In molasses medium the fat content of felt dropped to 24.4% and the fat coefficient to 6.2. Removal of inorganic materials from the molasses increased fat yields substantially, and the addition of molasses ash to the defined medium reduced yields to about the same level as in untreated molasses.

A chemically defined medium for Treponema strain PR-7 isolated from the intestine of a pig with swine dysentery

1972 ◽  
Vol 18 (7) ◽  
pp. 1073-1078 ◽  
Author(s):  
Robert M. Smibert ◽  
Raymond L. Claterbaugh Jr
Keyword(s):  
Carbon Dioxide ◽  
Energy Source ◽  
Intestinal Tract ◽  
Defined Medium ◽  
Vitamin B ◽  
Chemically Defined Medium ◽  
Aminobenzoic Acid ◽  
Phenotypic Characteristics ◽  
Swine Dysentery ◽  
End Products

The nutrition of treponeme strain PR-7 isolated from the intestinal tract of a pig with swine dysentery was studied. The organism fermented arabinose, xylose, maltose, cellobiose, glucose, galactose, mannose, lactose, pectin, and starch. The end products of fermentation of glucose were major amounts of succinic acid, moderate amounts of acetic and formic acid, and a trace of lactic acid and ethanol.Strain PR-7 required glutamate, aspartate, proline, leucine, methionine, arginine, valine, alanine, serine, lysine, glycine, threonine, isoleucine, phenylalanine, tyrosine, histidine, tryptophan, glutamine, asparagine, and spermine. The organism also required nicotinamide, folic acid, pyridoxal, thiamine, riboflavin, pantothenate, choline, α-lipoic acid, and biotin. The fatty acids isobutyrate, n-valerate, acetate, and pyruvate were needed as well as ammonium sulfate. A fermentable energy source such as glucose was necessary for growth, as well as carbon dioxide. Heme was also required and the organism was stimulated by vitamin B12, ascorbic acid, ornithine, and para-aminobenzoic acid. Heme could be replaced by catalase, myoglobin, and peroxidase.Ten other treponemes that were isolated from the intestines of normal pigs as well as from pigs with swine dysentery and had phenotypic characteristics similar to strain PR-7 grew in the defined medium.

scholarly journals Physiological Study of Lactobacillus delbrueckii subsp. bulgaricus Strains in a Novel Chemically Defined Medium

2000 ◽  
Vol 66 (12) ◽  
pp. 5306-5311 ◽  
Author(s):  
Christian Chervaux ◽  
S. Dusko Ehrlich ◽  
Emmanuelle Maguin
Keyword(s):  
Carbon Sources ◽  
Streptococcus Thermophilus ◽  
Defined Medium ◽  
Lactobacillus Delbrueckii ◽  
Protein Labeling ◽  
Chemically Defined Medium ◽  
Phosphotransferase System ◽  
Nutritional Conditions ◽  
Labeling Method

ABSTRACT We developed a chemically defined medium called milieu proche du lait (MPL), in which 22 Lactobacillus delbrueckii subsp.bulgaricus (L. bulgaricus) strains exhibited growth rates ranging from 0.55 to 1 h−1. MPL can also be used for cultivation of other lactobacilli and Streptococcus thermophilus. The growth characteristics of L. bulgaricus in MPL containing different carbon sources were determined, including an initial characterization of the phosphotransferase system transporters involved. For the 22 tested strains, growth on lactose was faster than on glucose, mannose, and fructose. Lactose concentrations below 0.4% were limiting for growth. We isolated 2-deoxyglucose-resistant mutants from strains CNRZ397 and ATCC 11842. CNRZ397-derived mutants were all deficient for glucose, fructose, and mannose utilization, indicating that these three sugars are probably transported via a unique mannose-specific-enzyme-II-like transporter. In contrast, mutants of ATCC 11842 exhibited diverse phenotypes, suggesting that multiple transporters may exist in that strain. We also developed a protein labeling method and verified that exopolysaccharide production and phage infection can occur in MPL. The MPL medium should thus be useful in conducting physiological studies ofL. bulgaricus and other lactic acid bacteria under well controlled nutritional conditions.

The THI5 gene family of Saccharomyces cerevisiae: distribution of homologues among the hemiascomycetes and functional redundancy in the aerobic biosynthesis of thiamin from pyridoxine

Microbiology ◽  
2003 ◽  
Vol 149 (6) ◽  
pp. 1447-1460 ◽  
Author(s):  
Raymond Wightman ◽  
Peter A. Meacock
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Family ◽  
Biosynthetic Pathway ◽  
Growth Conditions ◽  
Anaerobic Growth ◽  
Gene Copy ◽  
Phylogenetic Study ◽  
Vitamin B ◽  
Mrna Levels ◽  
Quadruple Mutant

The THI5 gene family of Saccharomyces cerevisiae comprises four highly conserved members named THI5 (YFL058w), THI11 (YJR156c), THI12 (YNL332w) and THI13 (YDL244w). Each gene copy is located within the subtelomeric region of a different chromosome and all are homologues of the Schizosaccharomyces pombe nmt1 gene which is thought to function in the biosynthesis of hydroxymethylpyrimidine (HMP), a precursor of vitamin B1, thiamin. A comprehensive phylogenetic study has shown that the existence of THI5 as a gene family is exclusive to those yeasts of the Saccharomyces sensu stricto subgroup. To determine the function and redundancy of each of the S. cerevisiae homologues, all combinations of the single, double, triple and quadruple deletion mutants were constructed using a PCR-mediated gene-disruption strategy. Phenotypic analyses of these mutant strains have shown the four genes to be functionally redundant in terms of HMP formation for thiamin biosynthesis; each promotes synthesis of HMP from the pyridoxine (vitamin B6) biosynthetic pathway. Furthermore, growth studies with the quadruple mutant strain support a previous proposal of an alternative HMP biosynthetic pathway that operates in yeast under anaerobic growth conditions. Comparative analysis of mRNA levels has revealed subtle differences in the regulation of the four genes, suggesting that they respond differently to nutrient limitation.

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