VARIABILITY IN NUTRITIONAL VALUE OF FISH FLOUR

1961 ◽  
Vol 39 (3) ◽  
pp. 511-517 ◽  
Author(s):  
A. B. Morrison ◽  
J. M. McLaughlan

Corrected protein efficiency ratio (P.E.R.) values for various samples of fish flour were found to vary from 1.51 to 2.76. Coefficients of apparent protein digestibility were similar for all flours tested, but the lysine in the sample which gave the lowest P.E.R. was less available by rat bio-assay than that in the other samples. Although the variation in nutritional value could not be ascribed to differences in the total lysine, methionine, and cystine contents of the samples, reduced amounts of lysine and methionine were released by pancreatic digestion of the flour which gave the lowest P.E.R. value. It was suggested that the reduced utilization of the amino acids in this sample may have resulted, in part, from a reduction in their rates of release in vivo. It was concluded that although fish flour may vary widely in nutritional value, properly prepared samples provide high quality protein for use in human or animal diets.


2015 ◽  
Vol 28 (4) ◽  
pp. 421-430 ◽  
Author(s):  
Cláudia Cantelli Daud BORDIN ◽  
Maria Margareth Veloso NAVES

Objective Although deficient in all indispensable amino acids, gelatin is used in protein-restricted diets. Food efficiency and protein quality of casein and gelatin mixtures in low protein diets in Wistar rats were investigated. Methods The rats were treated with protein-restricted diets (10.0 and 12.5%) containing casein (control diets), casein with gelatin mixtures (4:1 of protein content), and gelatin as sources of protein. The food conversion ratio, protein efficiency ratio, relative and corrected protein efficiency ratio, true protein digestibility, and hepatic parameters were estimated. Results After 28 days of the experiment, food efficiency of 10.0% casein/gelatin diet decreased when compared to that of 10.0% casein diet, and the protein efficiency ratio of the casein/gelatin mixtures (10.0%=2.41 and 12.5%=2.03) were lower than those of the casein (10.0%=2.90 and 12.5%=2.32). After 42 days of the experiment, the weight of the liver of the animals treated with 10.0 and 12.5% casein/gelatin diets, and the liver protein retention of the 12.5% casein/gelatin diet group of animals were lower than those of the control group. Conclusion Gelatin decreases food efficiency and high-quality protein bioavailability in protein-restricted diets.



Author(s):  
S. Shpуnova ◽  
O. Yadrishchenskaya ◽  
T. Selina ◽  
E. Basova

In recent years, the Russian poultry industry has been successfully developing because of the use of modern crosses, as well as technologies for poultry housing and feeding. The development of quail farming as an eff ective source of dietary and delicacy products is promising. One of the most important conditions for successful breeding of quails is the organization of complete feeding, which involves the use of high-quality protein and energy feed in the diets. It is very important to maximize the use of local raw materials. In this series, a special place is occupied by hulless barley, which is characterized by a reduced content of hard-to-digest fi ber, and therefore has the increased nutritional value for poultry. Therefore, it is of particular relevance to study the input of various varieties of hulless barley in the composition of compound feed for poultry. The purpose of the research was to study the infl uence of compound feed using hulless barley of Siberian selection on the meat productivity of quails. The results of the study on quails using compound feed with 10 % hulless barley have been presented in the article. It has been found that the expenditures of feed per 1 kg of live weight gain in the experimental group of quails have been higher by 3,6 % due to better assimilation of feed nutrients. The live weight of quails in the experimental group has been increased by 4,31 %, while the average daily feed intake has been increased by 0,62 %. Data from the balance experiment have shown that protein digestibility has increased by 1,81 % in the experimental group. It has been found that the use of compound feed with 10 % of hulless barley is of practical signifi cance in relation with the increase in the productivity of quails and the profi tability of meat production by 8,3 %.



2020 ◽  
Vol 45 (1) ◽  
pp. 325-334
Author(s):  
R. Y. Aderinboye ◽  
A. A. Busari ◽  
O. N. Olaoye ◽  
O. O. Adelusi ◽  
K. O Adebayo ◽  
...  

Effect of cashew nut shell liquid (CNSL) in diets for West African dwarf (WAD) goats on protein digestibility and utilization was evaluated in vitro and in vivo. Four diets consisting of Panicum maximum supplemented with concentrate pellets containing 0, 5, 10 and 15mL/kg of CNSL were used. Panicum and pellets were combined in ratio 70: 30 of required dry matter (DM). For in vitro experiment, 500 mg (n=8 per diet) of diet samples (DM basis) were incubated at 39°C for 48 h following the procedure of Menke and Steingass (1988). Dry matter and crude protein digestibility, ammonia nitrogen (NH3-N) and bacteria count were determined after 24 and 48 h incubation by recovering feed residues and rumen liquor. For in vivo experiment, twenty-four WAD goats were divided into four groups of six animals each. Each group was fed one of the four diets at 5% body weight (DM basis). Dry matter and crude protein intake, weight gain, protein efficiency ratio, rumen NH3-N, bacteria count and crudeprotein digestibility were measured during 98 days of feeding and digestibility trial. Experiments were arranged in a completely randomized design and data analyzed using one way analysis of variance procedure of SAS (1999). Results showed that 5 – 15 mL/kg CNSL in supplemental pellets reduced (P < 0.05) protein digestibility in vitro but increased (P < 0.05) total-tract protein digestibility in vivo. In vitro rumen NH3-N decreased (P < 0.05) with 5 – 15ml CNSL inclusion after 24 and 48 h. At 30 and 60 days post-feeding, 10 – 15 mL CNSL reduced (P < 0.05) rumen NH3-N in goats Protein efficiency ratio was higher (P < 0.05) with CNSL and goats fed 15 mL CNSL had the highest (P < 0.05) protein efficiency ratio. Rume bacteria population in vitro and in vivo decreased (P < 0.05) with 5 – 15 mL CNSL in supplemental pellets. In conclusion, the reduced in vitro protein digestibility with reduced NH3-Nproduction and bacteria population in vitro and in vivo suggests an inhibitory effect of CNSL on rumen proteolysis. Cashew nut shell liquid in supplemental pellet for WAD goats up to 15 mL/kg therefore inhibited dietary protein breakdown in the rumen with consequent improvement in protein digestibility and utilization at the lower tract.



1967 ◽  
Vol 45 (11) ◽  
pp. 1673-1679 ◽  
Author(s):  
E. M. Olsen

Five samples of wheat germ meal (WGM): raw, toasted 45 min, autoclaved 20, 45, or 90 min were analyzed for amino acids. Arginine and lysine were decreased by all treatments; the greatest loss was of lysine in 45- and 90-min autoclaved samples. Digestibility of protein and absorption of amino acids, determined with rats, was decreased by autoclaving. Decreased amino acid content and protein digestibility was reflected in lower weight gain and protein efficiency ratio (PER). Experimentally determined protein digestibilities of mixtures of raw and 90-min autoclaved WGM showed that the digestibility values of the two components were additive. Supplementation of raw WGM with amino acids showed that only methionine improved growth and PER. By these criteria, methionine-supplemented raw WGM was superior to methionine-supplemented soybean meal. Raw WGM exhibited no toxicity for the rat. The results confirm the excellent quality of protein in WGM. It is suggested that heat treatments applied during processing should be kept at a minimum.



2018 ◽  
Vol 52 (3) ◽  
Author(s):  
Raycha Lei Concess M. Rama ◽  
Jillean Camille Q. Fabi ◽  
Generose Christy M. Mateo ◽  
Jewel Amor C. Catubag ◽  
Lemuel L. Lozada ◽  
...  

Objective. This study has been conducted to determine the protein efficiency ratio (PER) of selected indigenous legumes, namely pigeon pea (Cajanus cajan) and lima bean (Phaseolus lunatus), as alternative protein sources. Methods. Experimental diets (pigeon pea-based and lima bean-based) and control diet (casein-based) were formulated and standardized using proximate analysis to have 10% protein basal diet based on the Association of Official Analytical Chemists (AOAC) guidelines. Diets were given to corresponding groups (2 experimental, 1 control) using 30 albino Sprague-Dawley weanling rats aged 21 days old, over a 28-day growth assay. Results. Results showed that the mean final weight and weight gain were significantly higher in rats fed with caseinbased diet (p<0.05 for both parameters) compared to those fed with pigeon pea-based diet and lima bean-based diet. The two experimental groups showed no significant difference between their mean final weights and weight changes (p=1, p>0.05). The PER of the casein (3.37 ± 2.71) is higher than that of the pigeon pea (1.87) and lima bean (1.32). These results can be attributed to the presence of anti-nutritional factors (ANFs) in the seeds of pigeon pea and lima bean. Reduced protein intake (due to bitter taste), toxicity, and interference with protein digestibility (through interaction with digestive enzymes) imparted by these ANFs can explain the weight loss among the experimental groups and consequently the low PER. Heat treatment done in the study were not adequate to remove the ANFs. Conclusion. The study shows that pigeon pea and lima bean as protein sources alone are not as adequate and efficient in providing necessary protein requirements for weanling rats. Proper processing and treatment should be done to remove inhibitors of protein digestibility and quality.



1982 ◽  
Vol 65 (4) ◽  
pp. 798-809 ◽  
Author(s):  
Lowell D Satterlee ◽  
James G Kendrick ◽  
Henry F Marshall ◽  
Duane K Jewell ◽  
Rida A Ali ◽  
...  

Abstract Seven laboratories collaborated in testing the calculated protein efficiency ratio (C-PER and DC-PER). The collaborative study required each laboratory to analyze 6 foods and a control protein (ANRC casein) for in vitro apparent protein digestibility, amino acid composition, and PER via rat bioassay. The 6 foods or food ingredients tested were nonfat dry milk, cooked chicken muscle, protein-fortified dry breakfast cereal, textured soy protein, oat-based dry breakfast cereal, and durum wheat flour. Data obtained from the study were analyzed statistically for the intralaboratory variation for each method of analysis (i.e., amino acid analysis, PER, etc.). The ability of the C-PER to rapidly predict rat PER was also measured. The C-PER and DC-PER methods were adopted official first action.



1979 ◽  
Vol 93 (1) ◽  
pp. 47-49 ◽  
Author(s):  
R. Rai ◽  
S. N. Singh

SummaryNine strains of Rhizobium sp. were studied in vivo for their nodulation capacity, leghaemoglobin content, grain yield, crude protein and 16 amino acids content, in the chick pea variety C 235 grown on a calcareous saline alkali soil. There was no significant correlation between grain yield and number of nodules (r = 0·37) or dry weight of nodules (r = 0·29), but grain yield was significantly correlated with leghaemoglobin content of nodules (r = 0·95). Of the 16 amino acids analysed in seed samples, aspartic, glutamic, proline and histidine were greatest with strain H 45; glycine, leucine and arginine with strain F 6; norleucine, tyrosine and phenylalanine with strain KG 38; and alanine and valine were greatest with strain KG 41. Strain KG 38 led to significantly higher grain yield than the other strains.



Author(s):  
Jimena A Ibagon ◽  
Su A Lee ◽  
Hans H Stein

Abstract The objective was to test the hypothesis that there is no effect of origin or processing procedure on the standardized ileal digestibility (SID) of amino acids (AA) and crude protein (CP) in sunflower co-products. Six sources of sunflower meal (SFM) and 1 source of sunflower expellers (SFE) were obtained from Ukraine, Italy, Hungary, and the U.S. Each source of SFM or SFE was the only source of CP and AA in 1 diet and an N-free diet was also used for a total of 8 diets. Eight barrows (body weight: 28.5 ± 2.4 kg) had a T-cannula installed in the distal ileum and were allotted to 1 of the 8 diets using an 8 × 8 Latin square design with 8 periods. The Lys:CP ratio in the 6 sources of SFM ranged from 3.10 to 3.96% with CP concentrations ranging from 27.34 to 36.75%. Crude protein in SFE was 26.87% and the Lys:CP ratio was 3.51%. Concentrations of acid hydrolyzed ether extract in the 6 sources of SFM ranged from 0.60 to 3.11%, but SFE contained 8.77%. Results indicated that the SID of CP was lower (P &lt; 0.05) in SFM from Hungary compared with all other sources of SFM except for 1 of the sources from Ukraine. There were no differences in the SID of Lys, Met, and Trp among sources of SFM, but for most of the remaining indispensable AA, the SFM from Hungary had less (P &lt; 0.05) SID than the other sources. However, only few differences in SID of indispensable AA were observed among the other sources of SFM, but the SID of CP and all AA except Trp was greater (P &lt; 0.05) in SFE compared with SFM. In conclusion, there were only few differences in the SID of the first limiting AA among SFM obtained from Ukraine, Hungary, Italy, and the U.S., but the SID of CP and AA was greater in SFE than in SFM indicating that processing of sunflower seeds influence the nutritional value.



Nutrients ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1776 ◽  
Author(s):  
Masresha Tessema ◽  
Nilupa Gunaratna ◽  
Inge Brouwer ◽  
Katherine Donato ◽  
Jessica Cohen ◽  
...  

Limited evidence is available on the associations of high-quality protein and energy intake, serum transthyretin (TTR), serum amino acids and serum insulin-like growth factor-1 (IGF-1) with linear growth of young children. Data collected during the baseline of a randomized control trial involving rural Ethiopian children aged 6–35 months (n = 873) were analyzed to evaluate the associations among height/length-for-age z-scores, dietary intakes, and these biomarkers (i.e., serum level of TTR, IGF-1, tryptophan and lysine, and inflammation). The prevalence of stunting was higher for children >23 months (38%) than ≤23 months (25%). The prevalence of inflammation was 35% and of intestinal parasites 48%. Three-quarters of the children were energy deficient, and stunted children had lower daily energy intake that non-stunted children (p < 0.05). Intakes of tryptophan, protein, and energy, and serum levels of tryptophan and IGF-1 were positively correlated with the linear growth of children. Controlling for inflammation, intestinal parasites, and sociodemographic characteristics, daily tryptophan (b = 0.01, p = 0.001), protein (b = 0.01, p = 0.01) and energy (b = 0.0003, p = 0.04) intakes and serum TTR (b = 2.58, p = 0.04) and IGF-1 (b = 0.01, p = 0.003) were positively associated with linear growth of children. Linear growth failure in Ethiopian children is likely associated with low quality protein intake and inadequate energy intake. Nutrition programs that emphasize improved protein quantity and quality and energy intake may enhance the linear growth of young children and need to be further investigated in longitudinal and interventional studies.



2019 ◽  
Vol 150 (3) ◽  
pp. 450-457 ◽  
Author(s):  
Romain Tessier ◽  
Nadezda Khodorova ◽  
Juliane Calvez ◽  
Romain Kapel ◽  
Alain Quinsac ◽  
...  

ABSTRACT Background In the context of developing plant protein sources for humans, sunflower is a good candidate in its form as an oilseed coproduct. Objectives We aimed to compare the real digestibility in rats of a sunflower isolate to that of goat whey protein. We also studied the efficiency of 15N and 2H intrinsic labeling in this assessment. Methods Sunflower seeds and goat milk were labeled with 15N and 2H. Male Wistar rats (10 wk old) were fed a meal containing 12% of either sunflower isolate (n = 8) or whey (n = 8). Six hours after meal ingestion, protein and amino acid digestibility were assessed by measuring nitrogen, hydrogen, and amino acids in the digesta, as well as isotope enrichments in the bulk and individual amino acids. The differences between groups and isotopes were respectively tested with an unpaired and a paired t test. Results Protein isolate purity was 87% for whey and 94% for sunflower. 2H and 15N enrichments were, respectively, 0.12 atom % (AP) and 1.06 AP in sunflower isolate and 0.18 AP and 0.95 AP in whey. Fecal 15N protein digestibility was 97.2 ± 0.2% for whey and 95.1 ± 0.5% for sunflower isolate. The use of 2H resulted in a lower digestibility estimate than 15N for whey (96.9 ± 0.2%, P &lt; 0.05) and sunflower (94.2 ± 0.5%, P &lt; 0.01). For both isotopes, protein digestibility was about 2% higher for whey than for sunflower isolate. Mean 15N amino acid caecal digestibility was 97.5 ± 0.2% for whey and 96.3 ± 0.2% for sunflower isolate. The values obtained with 15N and 2H resulted in significant differences ranging from −0.1% to 3.5%. The DIAAS was &gt;1.0 for whey and 0.84 for sunflower (lysine). Conclusions The protein and amino acid digestibility of sunflower isolate was high but its DIAAS reflected a moderate lysine imbalance. Despite slight differences with 15N, deuterium produced comparable results, making it suitable for in vivo digestion studies.



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