A comparison of some values of blood samples from heart and tail vessels of the Urodele, Taricha granulosa

1970 ◽  
Vol 48 (4) ◽  
pp. 900-901 ◽  
Author(s):  
G. B. Friedmann
Keyword(s):  
Haemoglobin Level ◽  
Cell Count ◽  
Blood Parameters ◽  
Taricha Granulosa ◽  
Blood Samples ◽  
Cell Counts ◽  
Heart Blood ◽  
Two Parameters ◽  
Tail Blood

A comparison of the blood parameters, cell count, and haemoglobin level for heart and tail blood of adult Taricha granulosa is presented. Data from 10 pairs of cell counts and 7 pairs of haemoglobin levels indicate that heart blood yields values only some three-quarters as large as does tail blood for these two parameters.

The annual cycle of red blood cell count and haemoglobin level in the urodele Taricha granulosa on southern Vancouver Island

1974 ◽  
Vol 52 (4) ◽  
pp. 487-494 ◽  
Author(s):  
G. B. Friedmann
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Red Blood Cell ◽  
Annual Cycle ◽  
Field Data ◽  
Blood Cell Count ◽  
Taricha Granulosa ◽  
Cell Counts ◽  
Red Blood Cell Count ◽  
Good Agreement

Appreciable, if unspectacular, seasonal variations in red blood cell count, rather than in haemoglobin levels, is shown to be a reasonable indicator of the position of (male) Taricha granulosa during the animal's annual cycle at sea level, for southern Vancouver Island.Animals collected in the field over a 17-month period yielded 274 cell counts and 363 haemoglobin values with average values of (8.0 × 104 ± 15%) cells/mm3 blood and (8.5 ± 6%) g/100 ml blood, respectively. Depressed values of these averages were encountered in late summer.Animals acclimated to laboratory conditions at 15 °C and kept for a few months gave values in good agreement with the field data.

Intestinal Parasites Infections among HIV Infected Children Under Antiretrovirals Treatment in Yaounde, Cameroon

2019 ◽  
Vol 66 (2) ◽  
pp. 178-186
Author(s):  
William Baiye Abange ◽  
Celine Nguefeu Nkenfou ◽  
Hortense Gonsu Kamga ◽  
Clement Assob Nguedia ◽  
Nelly Kamgaing ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Count ◽  
Intestinal Parasites ◽  
Parasitic Infection ◽  
Cd4 T Cell ◽  
Parasitic Infections ◽  
Blood Samples ◽  
Cryptosporidium Spp ◽  
Cell Counts ◽  
Cd4 T Cell Count

Abstract Background Intestinal parasitic infections are among the most common communicable diseases worldwide, particularly in developing countries. Human immunodeficiency virus (HIV) causes dysregulation of the immune system through the depletion of CD4+ T lymphocytes which gives rise to opportunistic infections. Methodology A cross-sectional study was conducted from January to October 2018. Stool and blood samples were collected from participants aged 1 to 19. Stool samples were analyzed for intestinal parasites. Blood samples were analyzed for HIV and CD4 + T cell counts. Results Out of 214 children enrolled, 119 (55.6%) were HIV infected and 95 (44.4%) were HIV non-infected. All infected children were on antiretroviral treatment (ART). The prevalence of intestinal parasites was 20.2% in HIV infected and 15.8% in non-infected children. Among the 119 HIV infected children, 33 (27.7%) of them had a CD4+ T cell count less than 500 cells/mm3, and amongst them 5.9% had CD4+ T cell count less than 200 cells/mm3. Among HIV infected children, Cryptosporidium spp. was frequently detected, 7/119 (5.9%), followed by Giardia lamblia 5/119 (4.2%) then Blastocystis hominis 3/119 (2.5%) and Entamoeba coli 3/119 (2.5%). Participants on ART and prophylactic co-trimoxazole for >10 years had little or no parasite infestation. Conclusions Although ART treatment in combination with prophylactic co-trimoxazole reduces the risk of parasitic infection, 20.2% of HIV infected children harbored intestinal parasites including Cryptosporidium spp. Stool analysis may be routinely carried out in order to treat detected cases of opportunistic parasites and such improve more on the life quality of HIV infected children.

Altitudinal variations in the red blood cell count and haemoglobin content of urodele blood

1971 ◽  
Vol 49 (4) ◽  
pp. 565-568 ◽  
Author(s):  
G. B. Friedmann
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Haemoglobin Concentration ◽  
Taricha Granulosa ◽  
Average Cell ◽  
Cell Counts ◽  
Group A ◽  
Red Blood Cell Count ◽  
Group B ◽  
Haemoglobin Content

Blood cell counts and haemoglobin levels for blood collected by tail amputation from three groups of tailed amphibians (Taricha granulosa) are presented. All animals were collected from very similar latitudes and longitudes, but different altitudes; acclimatization to the laboratory was for 6 to 8 months. For the calendar period January–March, group A from an altitude of 3400 ft yielded an average cell count of 7.58 (s.d. 1.63) × 104/mm3 and an average haemoglobin content of 6.0 (1.1) g/100 ml blood; group B from 1300 ft yielded an average cell count of 8.92(1.73) × 104/mm3 and an average haemoglobin content of 7.2 (1.4) g/100 ml blood while group C from sea level gave an average cell count of 9.51 (1.81) × 104/mm3 and an average haemoglobin content of 7.5 (1.5) g/100 ml blood. The results for group A differ statistically from those of groups B and C. In all cases, the haemoglobin concentration is 0.77 (0.10) × 10−9 g/cell.

scholarly journals Effect of helminthosis on some blood parameters in pigs in Ibadan, Nigeria

2020 ◽  
Vol 47 (1) ◽  
pp. 53-57
Author(s):  
E. A. Adenaike
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Blood Parameters ◽  
Blood Parasites ◽  
Helminth Parasites ◽  
Blood Cell Count ◽  
Large White ◽  
Blood Samples ◽  
Mean Values ◽  
The Mean

Coprological and haematological investigations were carried out on faecal and blood samples collected from 154 pigs from various locations around Ibadan metropolis. Coprological examination consisted mainly of egg floatation techniques while haematological investigations consisted of packed cell volume (PCV), red blood cell count (RBC) and white blood cell count (WBC). Microscopic examination of smears of the blood samples for protozoan blood parasites was also carried out. Faecal samples of 56 (36.36%) pigs were positive for helminth parasites while all the blood samples were negative for protozoan parasites. The eggs of helminth parasites found in this study were those of Ascaris spp., Metastrongylus spp., Globocephallus spp., Oesophagostomum spp., Paragonimus spp., Hyostrongylus spp. and Trichuris spp. The mean PCV, RBC and WBC values of pigs negative for helminth parasites were 31.66±8.30%, 10.57±4.68 x106 /ml and 16.97±7.82 x106 /ml respectively while the values for positive pigs were 29.71±8.93%, 9.81±4.84 x109 /ml and 17.34±7.24 x106 /ml respectively. Helminthosis caused a decline in the mean values of PCV and RBC count. The percentage decline in mean PCV values was 6.13%, 8.43% and 1.64% respectively for large white, large white crossed and local breeds of pigs. The percentage decline in mean RBC values was 6.97%, 8.74% and 3.01% respectively for large white, large white crossed and local breeds of pigs. Helminthosis caused an increase in the mean WBC values in the three breeds which was 2.69%, 1.22% and 0.91% respectively for large white, large white crossed and local breeds of pigs. The implications of the findings are discussed. In conclusion, helminthosis caused a decline in the mean values of PCV and RBC values resulting in anaemia in all breeds of pigs while the mean WBC count increased. However, experimental infections of various breeds of pigs of different ages by various types of parasites need to be carried out in order to confirm the trend observed in this study.

A quantitative evaluation of some factors affecting the non-fatty solids of cow's milk

1960 ◽  
Vol 27 (1) ◽  
pp. 19-32 ◽  
Author(s):  
W. H. Alexander ◽  
F. B. Leech
Keyword(s):  
Cell Count ◽  
Residual Effect ◽  
Clinical Mastitis ◽  
High Cell ◽  
Factors Affecting ◽  
Stage Of Lactation ◽  
Cell Counts ◽  
Satisfactory Analysis ◽  
The Difference ◽  
Image Position

SummaryTen farms in the county of Durham took part in a field study of the effects of feeding and of udder disease on the level of non-fatty solids (s.n.f.) in milk. Statistical analysis of the resulting data showed that age, pregnancy, season of the year, and total cell count affected the percentage of s.n.f. and that these effects were additive and independent of each other. No effect associated with nutritional changes could be demonstrated.The principal effects of the factors, each one freed from effects of other factors, were as follows:Herds in which s.n.f. had been consistently low over a period of years were compared with herds in which s.n.f. had been satisfactory. Analysis of the data showed that about 70% of the difference in s.n.f. between these groups could be accounted for by differences in age of cow, stage of lactation, cell count and breed.There was some evidence of a residual effect following clinical mastitis that could not be accounted for by residual high cell counts.The within-cow regression of s.n.f. on log cell count calculated from the Durham data and from van Rensburg's data was on both occasions negative.The implications of these findings are discussed, particularly in relation to advisory work.

scholarly journals Frequency of Cytomegalovirus Viral Load in Iranian Human Immunodeficiency Virus-1-Infected Patients with CD4+ Counts <100 Cells/mm3

Intervirology ◽  
2021 ◽  
pp. 1-5
Author(s):  
Mohammad Reza Jabbari ◽  
Hoorieh Soleimanjahi ◽  
Somayeh Shatizadeh Malekshahi ◽  
Mohammad Gholami ◽  
Leila Sadeghi ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Viral Load ◽  
Cell Count ◽  
Previous History ◽  
Cd4 Count ◽  
Cd4 Cell Count ◽  
Cell Counts ◽  
Immunodeficiency Virus ◽  
Cd4 Cell ◽  
Hiv 1

<b><i>Objectives:</i></b> The aim of present work was to assess cytomegalovirus (CMV) viremia in Iranian human immunodeficiency virus (HIV)-1-infected patients with a CD4+ count &#x3c;100 cells/mm<sup>3</sup> and to explore whether CMV DNA loads correlate with CD4+ cell counts or associated retinitis. <b><i>Methods:</i></b> This study was conducted at the AIDS research center in Iran on HIV-1-infected patients with CD4+ count &#x3c;100 cells/mm<sup>3</sup>, antiretroviral therapy-naive, aged ≥18 years with no previous history of CMV end-organ disease (CMV-EOD). <b><i>Results:</i></b> Thirty-nine of 82 patients (47.56%) had detectable CMV viral load ranging from 66 to 485,500 IU/mL. CMV viral load in patients with retinitis ranges from 352 to 2,720 IU/mL, and it was undetectable in 2 patients. No significant associations between CMV viremia and CD4+ cell count was found (<i>p</i> value = 0.31), whereas significant association of CMV viremia in HIV-infected patients with retinitis was found (<i>p</i> &#x3c; 0.02). <b><i>Conclusions:</i></b> We estimated the frequency of CMV viral load infection in Iranian HIV-1-infected patients with a CD4+ cell count &#x3c;100 mm<sup>3</sup>/mL in the largest national referral center for HIV-1 infection in Iran. Further research is required on the relevance of CMV viral load in diagnostic and prognostic value of CMV-EOD.

scholarly journals CD19 Cell Count at Baseline Predicts B Cell Repopulation at 6 and 12 Months in Multiple Sclerosis Patients Treated with Ocrelizumab

2021 ◽  
Vol 18 (15) ◽  
pp. 8163
Author(s):  
Gianmarco Abbadessa ◽  
Giuseppina Miele ◽  
Paola Cavalla ◽  
Paola Valentino ◽  
Girolama Alessandra Marfia ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Count ◽  
Considerable Proportion ◽  
Cell Counts ◽  
Time Scheduling ◽  
Magnetic Resonance Imaging Mri ◽  
Multiple Sclerosis Patients ◽  
Kinetics Of ◽  
Cell Repopulation

Background: The kinetics of B cell repopulation in MS patients treated with Ocrelizumab is highly variable, suggesting that a fixed dosage and time scheduling might be not optimal. We aimed to investigate whether B cell repopulation kinetics influences clinical and radiological outcomes and whether circulating immune asset at baseline affects B cell repopulation kinetics. Methods: 218 MS patients treated with Ocrelizumab were included. Every six months we collected data on clinical and magnetic resonance imaging (MRI) activity and lymphocyte subsets at baseline. According to B cell counts at six and twelve months, we identified two groups of patients, those with fast repopulation rate (FR) and those with slow repopulation rate (SR). Results: A significant reduction in clinical and radiological activity was found. One hundred fifty-five patients had complete data and received at least three treatment cycles (twelve-month follow-up). After six months, the FR patients were 41/155 (26.45%) and 10/41 (29.27%) remained non-depleted after twelve months. FR patients showed a significantly higher percentage of active MRI scan at twelve months (17.39% vs. 2.53%; p = 0,008). Furthermore, FR patients had a higher baseline B cell count compared to patients with an SR (p = 0.02 and p = 0.002, at the six- and twelve-month follow-ups, respectively). Conclusion: A considerable proportion of MS patients did not achieve a complete CD19 cell depletion and these patients had a higher baseline CD19 cell count. These findings, together with the higher MRI activity found in FR patients, suggest that the Ocrelizumab dosage could be tailored depending on CD19 cell counts at baseline in order to achieve complete disease control in all patients.

scholarly journals Lymphocyte subset analysis to evaluate the prognosis of HIV-negative patients with pneumocystis pneumonia

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Fan Jin ◽  
Jing Xie ◽  
Huan-ling Wang
Keyword(s):  
T Cell ◽  
Cell Count ◽  
Lymphocyte Subsets ◽  
Nk Cell ◽  
Cd8 T Cell ◽  
Pneumocystis Pneumonia ◽  
Lymphocyte Subset ◽  
Cd4 T Cell ◽  
Cell Counts ◽  
Hiv Negative

Abstract Objectives We analysed the peripheral blood lymphocyte subsets of human immunodeficiency virus (HIV)-negative patients infected with pneumocystis pneumonia (PCP) to determine the relationships between the levels of different types of lymphocytes and the prognosis of patients. Methods We retrospectively reviewed HIV-negative patients with PCP diagnosed in our department. All the eligible patients underwent lymphocyte subset analysis on admission. Results A total of 88 HIV-negative PCP patients were enrolled in the study. In univariate analyses, low CD4+ T cell count, low CD8+ T cell count, and low natural killer cell (NK cell) count were associated with higher in-hospital mortality. CD8+ T cell count ≤300/μL was found to be an independent risk factor for poor prognosis in multivariate logistical regression analysis (p = 0.015, OR = 11.526, 95% CI = 1.597–83.158). Although low CD4+ T cell and NK cell counts were not independent risk factors, the mortality rates of PCP patients decreased as the CD4+ T cell and NK cell counts increased. Conclusion The immune process of Pneumocystis jirovecii infection is complex but important. We propose that lymphocyte subsets could give clinicians a better understanding of patient immune status, helping with the early identification of potentially lethal infections and treatment decision making, such as adjusting the immunosuppressive regimen and choosing an appropriate patient monitoring level.

scholarly journals The Graphical Representation of Cell Count Representation; a New Procedure for the Diagnosis of Periprosthetic Joint Infections

Antibiotics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 346
Author(s):  
Bernd Fink ◽  
Marius Hoyka ◽  
Elke Weissbarth ◽  
Philipp Schuster ◽  
Irina Berger
Keyword(s):  
Cell Count ◽  
Graphical Representation ◽  
Diagnostic Value ◽  
Cell Counting ◽  
Wear Particles ◽  
Type Iv ◽  
Joint Infections ◽  
Cell Counts ◽  
Matrix Assessment ◽  
Wbc Count

Aim: This study was designed to answer the question whether a graphical representation increase the diagnostic value of automated leucocyte counting of the synovial fluid in the diagnosis of periprosthetic joint infections (PJI). Material and methods: Synovial aspirates from 322 patients (162 women, 160 men) with revisions of 192 total knee and 130 hip arthroplasties were analysed with microbiological cultivation, determination of cell counts and assay of the biomarker alpha-defensin (170 cases). In addition, microbiological and histological analysis of the periprosthetic tissue obtained during the revision surgery was carried out using the ICM classification and the histological classification of Morawietz and Krenn. The synovial aspirates were additionally analysed to produce dot plot representations (LMNE matrices) of the cells and particles in the aspirates using the hematology analyser ABX Pentra XL 80. Results: 112 patients (34.8%) had an infection according to the ICM criteria. When analysing the graphical LMNE matrices from synovia cell counting, four types could be differentiated: the type “wear particles” (I) in 28.3%, the type “infection” (II) in 24.8%, the “combined” type (III) in 15.5% and “indeterminate” type (IV) in 31.4%. There was a significant correlation between the graphical LMNE-types and the histological types of Morawietz and Krenn (p < 0.001 and Cramer test V value of 0.529). The addition of the LMNE-Matrix assessment increased the diagnostic value of the cell count and the cut-off value of the WBC count could be set lower by adding the LMNE-Matrix to the diagnostic procedure. Conclusion: The graphical representation of the cell count analysis of synovial aspirates is a new and helpful method for differentiating between real periprosthetic infections with an increased leukocyte count and false positive data resulting from wear particles. This new approach helps to increase the diagnostic value of cell count analysis in the diagnosis of PJI.

The Effects of Acupuncture on White Cell Counts

1974 ◽  
Vol 02 (04) ◽  
pp. 383-398 ◽  
Author(s):  
Marjorie L. Brown ◽  
George A. Ulett ◽  
John A. Stern
Keyword(s):  
Cell Count ◽  
White Cell Count ◽  
Needle Insertion ◽  
White Cell ◽  
Acupuncture Points ◽  
Blood Cell Count ◽  
Cell Counts ◽  
Higher Temperature ◽  
Stimulation Of ◽  
Site Stimulation

The anticipation of acupuncture, simple insertion of needles or the electrical stimulation of needles at both classical acupuncture points and "false" points, all produce an increase in white blood cell count. Electrostimulation produced the greatest, expectation of needle insertion the least, increase in white cell count. Though needles remain to place, the white cell count returns to basal level within one hour. Preliminary data on peripheral skin temperature as affected by stimulation of acupuncture points and non-points, suggests a higher temperature on the side of stimulation. For acupuncture site stimulation, the temperature differential appears to be more persistent than is true when non-sites are stimulated. Subjects reported needle insertion at acupuncture points as less painful than at non-points. Feelings of numbness were produced by stimulation of both classical and false acupuncture points.

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