Effects of spaceflight on trabecular bone in rats

1983 ◽  
Vol 244 (3) ◽  
pp. R310-R314 ◽  
Author(s):  
W. S. Jee ◽  
T. J. Wronski ◽  
E. R. Morey ◽  
D. B. Kimmel
Keyword(s):  
Bone Marrow ◽  
Trabecular Bone ◽  
Wistar Rats ◽  
Proximal Tibia ◽  
Mineralized Tissue ◽  
Orbital Flight ◽  
Growth Cartilage ◽  
Skeletal Changes ◽  
Male Wistar Rats ◽  
Trabecular Bone Mass

Alterations in trabecular bone were observed in growing male Wistar rats after 18.5 days of orbital flight on the COSMOS 1129 biosatellite. Spaceflight induced a decreased mass of mineralized tissue and an increased fat content of the bone marrow in the proximal tibial and humeral metaphyses. The osteoblast population appeared to decline immediately adjacent to the growth cartilage-metaphyseal junction, but osteoclast numbers were unchanged. These results suggested that bone formation may have been inhibited during spaceflight, but resorption remained constant. With the exception of trabecular bone mass in the proximal tibia, the observed skeletal changes returned to normal during a 29-day postflight period.

scholarly journals Suplemen Calcitriol Dosis Tinggi Mencegah Osteoporosis dan Memicu Urolitiasis pada Tikus Ovariektomi

2021 ◽  
Vol 39 (2) ◽  
pp. 192
Author(s):  
Hartiningsih Hartiningsih ◽  
Devita Anggraeni
Keyword(s):  
Bone Marrow ◽  
Alkaline Phosphatase ◽  
Trabecular Bone ◽  
Wistar Rats ◽  
Proximal Tibia ◽  
Left Kidney ◽  
Ovariectomized Rats ◽  
High Dose ◽  
Left Tibia ◽  
Osteoporosis Risk

Calcitriol supplementation in ovariectomized rats for 8 weeks reduce osteoporosis risk and safe for kidney depend a proper dose. The objective of the research was to study the efectivity of high dose calcitriol for osteoporosis prevention and safe for kidney  in ovariectomized Wistar rats. Fifteen female Wistar rats at 8 weeks of age were randomly divided into three  groups of five which were sham operated rats (KN), ovariectomized rats (KOV), and ovariectomized rats+calcitriol supplementation 200ng/day (OVD200). After 8 weeks of treatment, blood samples were taken from plexus orbitalis medialis for estrogen analysis. All rats were then euthanized, left tibia and left kidney were taken for histopathological examination using hematoxylin and eosin stain. Immunohistochemistry using anti TRAP5b monoclonal antibody was also done for left tibia by streptavidin-biotin.  The results showed that estradiol level of KOV was significantly decreased compared with KN (P<0.05), meanwhile, estradiol levels of OVD200 were not significantly different compared with KOV. Histopathologic figure of kidney in KOV was not different compared with KN. Glomerulus was surrounded by Bowman‘s capsule, proximal and distal convoluted tubulus were lined with typical cuboidal epithelium. Immunohistochemistry result of proximal tibial epiphysis in KOV were shown more tartrate resistant alkaline phosphatase 5b (TRAP5b) expression in trabecular bone, which was located in bone marrow space, and trabecular speculum surface as well. Meanwhile, proximal tibia epiphysis in OVD200 had less tartrate resistant alkaline phosphatase 5b (TRAP5b) expression compared with KOV. Histopathologic figure  of proximal tibia epiphysis in group OVD200 was shown more trabecular bone speculum and less adipocyte in the bone marrow compared with KOV. Histopathologic figure of kidney in OVD200 was showed glomerular  hypertrophy and atrophy, as well as protein deposits in proximal convoluted tubules epithelium.  In conclusion, calcitriol supplementation 200ng/day in ovariectomized rats for 8 weeks prevented osteoporosis risk but trigger urolithiasis. Key words :  : Calcitriol, osteoporosis, ovariectomized rats, urolithiasis

Evaluation of Neuroendocrine and Immunological Parameters in Animals Following Chronic Combined Exposure to Xenobiotics

2021 ◽  
pp. 47-51
Author(s):  
AI Smolyagin ◽  
IV Mikhaylova ◽  
EV Ermolina ◽  
AA Stadnikov ◽  
VM Boev
Keyword(s):  
Bone Marrow ◽  
Wistar Rats ◽  
The Body ◽  
Combined Exposure ◽  
Immune Systems ◽  
Cell Counts ◽  
Apoptotic Protein ◽  
Occupational Setting ◽  
Male Wistar Rats ◽  
Ifn Γ

Background: A high prevalence of chromium and benzene compounds in the environment associated with motor vehicle and industrial operations arouses interest in the study of these xenobiotics in a long-term experiment. The objective of this work was to analyze effects of a chronic combined exposure to chromium and benzene on the hypothalamic-pituitary-adrenocortical (HPA) and immune systems of male Wistar rats. Materials and methods: Eighty male Wistar rats were administered potassium dichromate and benzene with drinking water in doses equaling one maximum permissible concentration (MPC) during 135 days. The hypothalamus, pituitary gland, adrenal glands, thymus, and spleen were then studied using morphometric, histological, and electron microscopy methods. The streptavidin-biotin peroxidase method was used to determine the expression of pro-apoptotic protein p53 and anti-apoptotic protein bcl2. We also measured the body, thymus and spleen weights of animals, nucleated cell counts in the thymus, spleen, and bone marrow and evaluated the cellular composition of the spleen and bone marrow as well as spontaneous and concanavalin A-induced secretion of interferon gamma (IFN-γ), IL-4, IL-6, and IL-10 cytokines by splenocytes. Results: We established an adverse effect of the exposure on the HPA function expressed in the activation of its secretory activity, blocking the release of hypothalamic neuropeptides at the level of the neurohypophysis and leading to ultrastructural damage to the neurosecretory cells of the hypothalamus, pituitary adenocytes and adrenal cortical cells, as well as in an increase in the programmed death of thymocytes. We also observed a decrease in the thymus weight and thymocyte counts and a complex of structural and functional changes indicating the status of its accidental involution in the exposed rats. The revealed decrease in the splenocyte count in the experimental group was accompanied by an increase in the size of the white spleen pulp. An increase in the induced production of the main immunoregulatory cytokines IFN-γ and IL-4 by splenocytes was found. Conclusion: Our findings can be used to analyze impairments of the HPA and immune systems in workers with a chronic combined exposure to benzene and chromium compounds in the occupational setting.

Effects of different intensities of strength and endurance training on some osteometabolic miRNAs, Runx2 and PPARγ in bone marrow of old male wistar rats

2019 ◽  
Vol 46 (2) ◽  
pp. 2513-2521
Author(s):  
Zahra Hemati Farsani ◽  
Ebrahim Banitalebi ◽  
Mohammad Faramarzi ◽  
Amin Bigham-Sadegh
Keyword(s):  
Bone Marrow ◽  
Endurance Training ◽  
Wistar Rats ◽  
Male Wistar Rats

Assessment of Cadmium Genotoxicity in Peripheral Blood and Bone Marrow Tissues of Male Wistar Rats

2009 ◽  
Vol 19 (2) ◽  
pp. 135-140 ◽  
Author(s):  
Ayla Çelik ◽  
Belgin Büyükakilli ◽  
Burak Çimen ◽  
Bahar Taşdelen ◽  
M. İkbal Öztürk ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Wistar Rats ◽  
Male Wistar Rats

scholarly journals Study of Humoral Factors Regulating the Production of Leukocytes. I. Demonstration of a "Neutropoietin" in the Plasma after Administration of Triamcinolone to Rats

Blood ◽  
1961 ◽  
Vol 17 (4) ◽  
pp. 434-443 ◽  
Author(s):  
SHU CHU SHEN ◽  
TAKASHI HOSHINO
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Wistar Rats ◽  
Significant Alteration ◽  
Humoral Factors ◽  
Group Iii ◽  
Group I ◽  
Male Wistar Rats ◽  
Group Ii ◽  
Blood Elements

Abstract Male Wistar rats were divided into 3 groups: Group I. Rats given the triamcinolone daily by gastric catheter all developed neutrophilia accompanied by lymphopenia. Group II. Rats given daily intraperitoneal injections of plasma from normal rats manifested no significant alteration in the peripheral blood elements. Group III. Rats given daily intraperitoneal injections of plasma from rats given triamcinolone invariably developed neutrophilia without lymphopenia. Studies of the bone marrow of these groups at the end of the experiments revealed increased myeloid:erythroid ratios in Groups I and III but not II. It is therefore believed that this experiment suggests the existence of a neutrophilia-promoting factor in the plasma following the administration of triamcinolone.

Histomorphometric analysis of rat skeleton following spaceflight

1987 ◽  
Vol 252 (2) ◽  
pp. R252-R255 ◽  
Author(s):  
T. J. Wronski ◽  
E. R. Morey-Holton ◽  
S. B. Doty ◽  
A. C. Maese ◽  
C. C. Walsh
Keyword(s):  
Bone Formation ◽  
Trabecular Bone ◽  
Proximal Tibia ◽  
Space Shuttle ◽  
Lumbar Vertebrae ◽  
Lumbar Vertebra ◽  
Histomorphometric Analysis ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Trabecular Bone Mass

Male Sprague-Dawley rats were placed in orbit for 7 days aboard the space shuttle. Bone histomorphometry was performed in the long bones and lumbar vertebrae of flight rats and compared with data derived from ground-based control rats. Trabecular bone mass was not altered during the 1st wk of weightlessness. Strong trends were observed in flight rats for decreased periosteal bone formation in the tibial diaphysis, reduced osteoblast size in the proximal tibia, and decreased osteoblast surface and number in the lumbar vertebra. For the most part, histological indexes of bone resorption were normal in flight rats. The results indicate that 7 days of weightlessness are not of sufficient duration to induce histologically detectable loss of trabecular bone in rats. However, cortical and trabecular bone formation appear to be diminished during the 1st wk of spaceflight.

scholarly journals The Effectiveness of Fresh Anchovies Diet and Calcitriol Supplementation to Prevent Osteoporosis in Ovariectomized Rats

2016 ◽  
Vol 10 (1) ◽  
Author(s):  
Hartiningsih H ◽  
Devita Anggraeni ◽  
Sudarminto S
Keyword(s):  
Bone Marrow ◽  
Trabecular Bone ◽  
Wistar Rats ◽  
Distal Femur ◽  
Ovariectomized Rats ◽  
Left Femur ◽  
Marrow Cavity ◽  
Femur Bone ◽  
Bone Marrow Cavity ◽  
Brown Color

This study was conducted to assess the effectiveness of a diet contained fresh anchovies and calcitriol supplementation for 6 weeks to prevent osteoporosis in ovariectomized rats. Fifteen Wistar rats aged 8 weeks were divided randomly into 3 groups (normal/K, ovariectomized/Ov, andovariectomized + calcitriol/OVD), 5 mice each. Group K and Ov rats were fed with fresh anchovies, while the OVD group was fed with freshanchovies + calcitriol. At the age of 15 weeks, all mice were done for euthanasia, then left femur was collected for immunohistochemistryexamination of tartrate resistant acid phosphatase5b (TRAP5b). The detection of (TRAP5b) was conducted using a monoclonal ant ibody antiTRAP5b, and detected using a streptavidin-biotin. The results showed that the metaphysis part of distal femur bone of mice group K, Ov, andOVD were positive TRAP5b stained with brown color on trabecular bone in bone marrow cavity and trabecular spiculum surface, but rats ingroup K and Ov had extensive bone marrow cavity and normal trabecular spiculum, whereas OVD group showed bone marrow cavity dilation,accumulation of adipocytes in the bone marrow cavity, and shorter the spiculum of trabeculae. It can be concluded that fresh anchovies diet andcalcitriol supplementation for 6 weeks are not effective in preventing osteoporosis in ovariectomized rats.Key words: calcitriol, femur, ovariohisterectomy

scholarly journals Bone marrow adipogenic lineage precursors (MALPs) promote osteoclastogenesis in bone remodeling and pathologic bone loss

2020 ◽  
Author(s):  
Wei Yu ◽  
Leilei Zhong ◽  
Lutian Yao ◽  
Yulong Wei ◽  
Tao Gui ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Loss ◽  
Trabecular Bone ◽  
Bone Remodeling ◽  
Mesenchymal Cell ◽  
Cell Network ◽  
Ovariectomized Mice ◽  
Drastic Increase ◽  
Trabecular Bone Mass ◽  
Bone Marrow Mesenchymal Cell

AbstractBone is maintained by coupled activities of bone-forming osteoblasts/osteocytes and bone-resorbing osteoclasts and an alternation of this relationship can lead to pathologic bone loss such as in osteoporosis. It is well known that osteogenic cells support osteoclastogenesis via synthesizing RANKL. Interestingly, our recently identified bone marrow mesenchymal cell population—marrow adipogenic lineage precursors (MALPs) that form a multi-dimensional cell network in bone—was computationally demonstrated to be the most interactive with monocyte-macrophage lineage cells through highly and specifically expressing several osteoclast regulatory factors, including RANKL. Using an adipocyte-specific Adipoq-Cre to label MALPs, we demonstrated that mice with RANKL deficiency in MALPs have a drastic increase of trabecular bone mass in long bones and vertebrae starting from 1 month of age but that their cortical bone is normal. This phenotype was accompanied by diminished osteoclast number and attenuated bone formation at the trabecular bone surface. Reduced RANKL signaling in calvarial MALPs also abolished osteolytic lesions after lipopolysaccharide (LPS) injections. Furthermore, in ovariectomized mice, elevated bone resorption was partially attenuated by RANKL deficiency in MALPs. In summary, our studies identified MALPs as a critical player in controlling bone remodeling during normal bone metabolism and pathological bone loss in a RANKL-dependent fashion.

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