Influence of temperature on blood pH of the human adult and newborn

1964 ◽  
Vol 19 (5) ◽  
pp. 897-900 ◽  
Author(s):  
Karlis Adamsons ◽  
Salha S. Daniel ◽  
Gillian Gandy ◽  
L. Stanley James
Keyword(s):  
Cell Concentration ◽  
Effect Of Temperature ◽  
Red Cell ◽  
Acid Base ◽  
Blood Ph ◽  
Influence Of Temperature On ◽  
The Mean ◽  
Ph Range ◽  
Two Populations

The effect of temperature upon pH of blood from adult and newborn humans was investigated. Although the mean ΔpH/ΔT values for the two populations differed, 0.0144/ °C for adults versus 0.0128 for the newborn, this was not peculiar to the source since the temperature-induced pH changes were identical when comparison was made between samples of similar pH and CO2 content. It was shown that even over the physiologic pH range ΔpH/ΔT is not constant but is a function of pH and CO2. No significant changes in ΔpH/ΔT were observed with oxygenation of hemoglobin. The influence of red cell concentration was detectable only when hematocrit values fell below 20%. A graph and an equation for the determination of ΔpH/ΔT of blood with a given pH and CO2 content is presented. acid base Submitted on December 9, 1963

Determination of the pH of Hemolyzed Packed Red Cells from Arterial Blood

1961 ◽  
Vol 7 (5) ◽  
pp. 536-541 ◽  
Author(s):  
May K Purcell ◽  
Gertrude M Still ◽  
Theodore Rodman ◽  
Henry P Close
Keyword(s):  
Blood Cell ◽  
Normal Subjects ◽  
The Body ◽  
Red Cell ◽  
Arterial Blood ◽  
Fiducial Probability ◽  
The Mean ◽  
Red Cell Ph

Abstract A technic is described for the determination of the in vivo pH of red blood cell hemolysates. The mean arterial red cell pH of 20 normal subjects was 7.19 with a range of 7.15 to 7.22. The fiducial probability at the 0.95 level is 7.13 to 7.25. The mean difference in pH between plasma and cells was 0.21, with a range of 0.15 to 0.23. It is suggested that changes in pH of erythrocytes may reflect changes in other less accessible cells of the body and that the determination may be a useful research and clinical procedure in the study of metabolic and respiratory derangements.

scholarly journals USING POTENTIOMETRIC ACID-BASE TITRATION TO DETERMINE PKA FROM MANGOSTEEN PERICARPS EXTRACT

2019 ◽  
Vol 16 (32) ◽  
pp. 768-773
Author(s):  
L. TANIA ◽  
C. DIAWATI ◽  
M. SETYARINI ◽  
N. KADARITNA ◽  
A. SAPUTRA
Keyword(s):  
Oxalic Acid ◽  
Potentiometric Titration ◽  
Accurate Determination ◽  
Acidity Constant ◽  
Equivalence Point ◽  
Acid Base ◽  
Titrant Volume ◽  
Acid Base Titration ◽  
Ph Range

One of the uses of acid-base indicators is to show the end point of the titration, so the accurate determination of acidity constant and pH range of indicators needs to be done. This study aims to determine the acidity constant (pKa) of mangosteen pericarp extract and its accuracy as an indicator of acid-base titration. Determination of pKa was done by a simple potentiometric titration method. The titration data were plotted in three graphs, i.e., pH, ΔpH/ΔV (the (the first derivative), and Δ2pH/ΔV2 (the second derivative) versus titrant volume to determine the equivalence point of the titration. The accuracy test was carried out by comparing the volume of oxalic acid used to titrate NaOH solution using the indicator of mangosteen pericarp extract and phenolphthalein indicator. The result showed that the equivalence point was found on the titrant volume of 8.6 mL and a measured pH of 9.84. so the pKa value of mangosteen pericarp extract was 7.20, and the pH range was 6.20 to 8.20. the average volume of oxalic acid used to titrate 5 mL of NaOH using phenolphthalein as the indicator was 5.2 mL while the titration used mangosteen pericarp extract was 5.23 mL. The accuracy of mangosteen pericarp extract was 99.42%. By the result, it can be concluded that potentiometric titration can be used as a simple way to determine the acidity constant of mangosteen pericarp extract. Moreover, the mangosteen pericarp extract can be used as an alternative acid-base titration indicator to substitute the common acid-base titration in the laboratory.

Significance of illite crystallinity and bo values of K-white mica in lowgrade metamorphic rocks, North Hill End Synclinorium, New South Wales, Australia

1985 ◽  
Vol 49 (352) ◽  
pp. 357-364 ◽  
Author(s):  
R. Offler ◽  
E. Prendergast
Keyword(s):  
Early Carboniferous ◽  
Geothermal Gradient ◽  
White Mica ◽  
Low Grade ◽  
Illite Crystallinity ◽  
The North ◽  
South Wales ◽  
The Mean ◽  
Two Populations

AbstractA study of low-grade metamorphism in late Silurian to early Carboniferous rocks in the North Hill End Synclinorium and adjacent anticlinoria has been made by the determination of illite crystallinity and bo values of K-white mica in eighty slates and phyllites. Illite crystallinity values vary from 0.40 Δ°2θ on the Molong Anticlinorium to 0.12 Δ°2θ within the axis of the synclinorium, suggesting anchizonal to epizonal metamorphic conditions. This is in agreement with previous observations on Ca-Al-hydrosilicate assemblages which indicated a change from prehnite-pumpellyite facies in the anticlinoria adjacent to the synclinorium to middle greenschist facies in the axis. Local variations in crystallinity are attributed to variation in ak+ in fluids migrating along cleavage zones.The mean bo value obtained from the pelites is 9.017 Å (σn = 0.008; n = 80) which is in close agreement with that obtained from part of the adjacent Capertee Anticlinorium (x̄ = 9.019 Å; σn = 0.007; n = 52). However, ‘t’ tests indicate that two bo populations are present in the synclinorium (x̄ = 9.019 and 9.022 Å), with the lower values concentrated in the southern portion of this structure. The two populations are considered to be the result of slightly different metamorphic conditions prevailing during the deformation of the rocks in the synclinorium. A higher geothermal gradient affecting rocks giving the lower bo values is attributed to the presence of granitoids at shallower depths than elsewhere in the synclinorium.

Increase in P50 with the use of Bicarbonate Hemodialysis

1987 ◽  
Vol 10 (6) ◽  
pp. 361-366
Author(s):  
G. Ramirez ◽  
G.L. Collice ◽  
S. James ◽  
C.C. Johns ◽  
W.P. Nelson
Keyword(s):  
Cell Volume ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Similar Amount ◽  
Red Cell ◽  
Dialysis Patients ◽  
Corpuscular Hemoglobin ◽  
Red Cell Volume ◽  
Blood Ph ◽  
The Mean

We studied the effect of bicarbonate and acetate on oxygenation during dialysis in ten male chronic dialysis patients. The dialysis delivery system and dialysate constituents were identical except for the use of either bicarbonate or acetate. We found no hemodynamic differences between the two kinds of dialysis. Blood PO2 fell by a similar amount, but blood PCO2 was higher during bicarbonate dialysis. The blood pH became alkalotic by the second hour of bicarbonate dialysis and remained so throughout the dialysis, whereas blood pH became alkalotic only at the end of acetate dialysis. The P50 increased significantly only during bicarbonate dialysis, but 2.3 DPG concentration did not change. Red cell volume, assessed by the mean corpuscular hemoglobin concentration, was unchanged. Without changes in the red cell volume we cannot explain the observed changes in P50 in the absence of concomitant changes in 2.3 DPG concentration.

scholarly journals Ionophore-based potentiometric PVC membrane sensors for determination of phenobarbitone in pharmaceutical formulations

2016 ◽  
Vol 66 (4) ◽  
pp. 503-514 ◽  
Author(s):  
Haitham Alrabiah ◽  
Abdulrahman Al-Majed ◽  
Mohammed Abounassif ◽  
Gamal A.E. Mostafa
Keyword(s):  
Limit Of Detection ◽  
Pharmaceutical Formulations ◽  
Dioctyl Phthalate ◽  
Pvc Membrane ◽  
Relative Standard ◽  
The Mean ◽  
Ph Range ◽  
Foreign Species ◽  
Good Agreement

Abstract The fabrication and development of two polyvinyl chloride (PVC) membrane sensors for assaying phenobarbitone sodium are described. Sensors 1 and 2 were fabricated utilizing β- or γ-cyclodextrin as ionophore in the presence of tridodecylmethylammonium chloride as a membrane additive, and PVC and dioctyl phthalate as plasticizer. The analytical parameters of both sensors were evaluated according to the IUPAC guidelines. The proposed sensors showed rapid, stable anionic response (-59.1 and -62.0 mV per decade) over a relatively wide phenobarbitone concentration range (5.0 × 10-6-1 × 10-2 and 8 × 10-6-1 × 10-2 mol L-1) in the pH range of 9-11. The limit of detection was 3.5 × 10-6 and 7.0 × 10-6 mol L-1 for sensors 1 and 2, respectively. The fabricated sensors showed high selectivity for phenobarbitone over the investigated foreign species. An average recovery of 2.54 μg mL-1 phenobarbitone sodium was 97.4 and 101.1 %, while the mean relative standard deviation was 3.0 and 2.1 %, for sensors 1 and 2, respectively. The results acquired for determination of phenobarbitone in its dosage forms utilizing the proposed sensors are in good agreement with those obtained by the British Pharmacopoeial method.

ACID-BASE EQUILIBRIUM OF BLOOD IN NORMAL INFANTS

PEDIATRICS ◽  
1966 ◽  
Vol 37 (5) ◽  
pp. 728-732
Author(s):  
Morris S. Albert ◽  
Robert W. Winters
Keyword(s):  
Base Excess ◽  
Capillary Blood ◽  
Age Group ◽  
Significant Extent ◽  
Acid Base ◽  
Base Equilibrium ◽  
Blood Ph ◽  
Acid Base Equilibrium ◽  
The Mean ◽  
Normal Acid

Acid-base equilibrium of arterialized capillary blood was determined on 139 normal infants between the ages of 3 months and 2 years. The mean and standard deviation of each component was as follows: pH 7.398 ± 0.027, base excess -3.2 ± 1.7 mEq/L, pCO2 33.8 ± 3.7 mm Hg, and plasma total CO2 content 21.1 ± 1.9 mM/L. When compared to normal adult values, all components except blood pH were reduced to a statistically significant extent. These data are proposed as definitive standards for normal acid-base equilibrium in infants of this age group.

Blood volume and red cell concentration in the normal chick embryo

1959 ◽  
Vol 197 (2) ◽  
pp. 403-405 ◽  
Author(s):  
Arthur E. Barnes ◽  
Wallace N. Jensen
Keyword(s):  
Chick Embryo ◽  
Blood Volume ◽  
Cell Concentration ◽  
Cell Mass ◽  
Somatic Growth ◽  
Red Cell ◽  
Total Blood ◽  
Red Cell Mass ◽  
Common Process

A method for the determination of red cell mass, which employs the use of radioisotopically-labeled erythrocytes, in the embryonated hen egg is described and results of total blood volume, plasma volume, red cell mass and red cell concentration determinations during the period from 9 to 18 days of incubation presented. Considerations of the values obtained in terms of absolute quantities and relative to embryonic mass are discussed. It is suggested that expansion of the red cell mass and somatic growth are portions of a common process and may be subject to a complex of similar regulatory factors in the normal chick embryo.

The Influence of Temperature on the Contribution of Awns to Yield in Wheat

1987 ◽  
Vol 14 (3) ◽  
pp. 299 ◽  
Author(s):  
LB Olugbemi ◽  
MG Bush
Keyword(s):  
Grain Growth ◽  
Flag Leaf ◽  
Dry Weight ◽  
Effect Of Temperature ◽  
Near Isogenic Lines ◽  
Grain Development ◽  
Photosynthetic Rates ◽  
Influence Of Temperature On ◽  
The Mean ◽  
Effect On Yield

The effect of temperature during grain set and grain growth on the contribution of awns to yield in wheat was examined in the Canberra phytotron. For eight pairs of awned and awnless near-isogenic lines, the mean advantage of the awned lines in grain dry weight per ear was greatest when plants were held at 21/16°C during grain development, less at 27/22°C and least at 15/10°C where grain weight per ear was highest. One pair, G16 (awned) and G18 (awnless), examined more closely, showed the same response with the yield advantage of the awned line tending to decline from 21/16°C to 33/28°C. There were no significant differences between G16 and G18 in photosynthetic rates of the flag leaves, but the presence of awns increased photosynthetic rates of the ears by up to 1.2 mg CO2 h-1 per ear. During the linear grain growth phase. the relative net contributions per hour by awns and flag leaf, neither of which were much affected by temperature, were about 1 : 10 in all treatments. It appeared probable that under well-watered conditions awns had an effect on yield only when assimilate supply was limiting. Awns did not always increase yield significantly in supra-optimal temperatures.

scholarly journals Accurate Estimation of Bicarbonate and Acetic Acid Concentrations with Wider Ranges in Anaerobic Media Using Classical FOS/TAC Titration Method

2021 ◽  
Vol 11 (24) ◽  
pp. 11843
Author(s):  
Xiaojun Liu ◽  
Laura André ◽  
Maël Mercier-Huat ◽  
Jean-Marie Grosmaître ◽  
André Pauss ◽  
...  
Keyword(s):  
Sodium Acetate ◽  
Fatty Acid Content ◽  
Total Alkalinity ◽  
Accurate Estimation ◽  
Acid Base ◽  
Titration Method ◽  
Simulation And Experiment ◽  
The Mean ◽  
Anaerobic Media

The determination of a volatile fatty acid content (FOS) and total alkalinity (TAC) can be carried out using Nordmann’s FOS/TAC titration method developed in the 1970s. This two-point titration (pH = 5 and 4.4) can be simply implemented and is widely employed by both the academic and industrial worlds. However, the present study proves that Nordmann’s method is only valid in limited ranges, since the titration of one FOS and TAC has an impact on the determination of the other, especially in extreme conditions. The present work develops a numerical tool with Scilab simulating the acid–base equilibria of titration. The program is efficient in predicting the experimental equivalent volumes obtained from Nordmann’s method with different combinations of sodium acetate and sodium bicarbonate contents. The mean absolute percentage errors (MAPE) between the simulation and experiment are below 7%. Two new formulas are developed, considering both equivalent volumes at pH = 5 and 4.4 to calibrate FOS and TAC values. The proposed formulas show their good performance in predicting various combinations of FOS and TAC contents in an anaerobic digestate at TAC ranging from 0 to 20,000 mg CaCO3·L−1 and FOS ranging from 0 to 31,000 mg HAc·L−1.

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