Effects of spaceflight on rat erythroid parameters

1996 ◽  
Vol 81 (1) ◽  
pp. 117-122 ◽  
Author(s):  
Z. Allebban ◽  
L. A. Gibson ◽  
R. D. Lange ◽  
T. L. Jago ◽  
K. M. Strickland ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Life Sciences ◽  
Lower Number ◽  
Ground Control ◽  
Colony Forming Unit ◽  
Human Erythropoietin ◽  
Group A ◽  
Group B ◽  
First Time ◽  
Group D

Hematologic studies were performed on 21 ground control rats and 21 rats flown during the Spacelab Life Sciences-2 14-day mission. Group A (n = 5) was used to collect blood in flight and 9 days postflight, group B (n = 5) was injected with recombinant human erythropoietin (rhEpo), group C (n = 5) received saline as a control, and group D (n = 6) was killed in flight and tissues were collected. Results indicated no significant changes in peripheral blood erythroid elements between flight and ground control rats. The nonadherent bone marrow on flight day 13 showed a lower number of recombinant rat interleukin-3 (rrIL-3)-responsive and rrIL-3 + rhEpo-responsive blast-forming unit erythroid (BFU-e) colonies in flight rats compared with ground control rats. On landing day, a slight increase in the number of rhEpo + rrIL-3-responsive BFU-e colonies of flight animals compared with ground control rats was evident. Nine days postflight, bone marrow from flight rats stimulated with rhEpo alone or with rhEpo + rrIL-3 showed an increase in the number of colony-forming unit erythroid colonies and a decrease in BFU-e colonies compared with ground control rats. This is the first time that animals were injected with rhEpo and subsequently blood and tissues were collected during the spaceflight to study the regulation of erythropoiesis in microgravity.

Liposomal Nanoparticle-Mediated miR-27b Influences Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells via Peroxisome Proliferator-Activated Receptor Gamma in a Microgravity Environment

2020 ◽  
Vol 12 (11) ◽  
pp. 1301-1308
Author(s):  
Zhiwei He ◽  
Yan Zhu ◽  
Gentao Fan ◽  
Hongbo Qian
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Simulated Microgravity ◽  
Mrna Level ◽  
Alp Activity ◽  
Marrow Mesenchymal Stem Cells ◽  
Group A ◽  
Group B ◽  
Group D

This study was aimed at analyzing the effects of liposomal nanoparticle-based miR-27b on PPARγ and osteogenic differentiation of bone marrow mesenchymal stem cells under microgravity. The rat bone marrow mesenchymal stem cells were set as the research object, and the gyroscope was employed for simulation of microgravity. The cells were randomized into four groups, including the experimental group A (simulated microgravity+liposomal nanoparticle-mediated miR-27b transfection group), as well as the control groups: group B (simulated microgravity+negative control group), group C (simulated microgravity+transfection reagent group) and group D (normal gravity+liposomal nanoparticle-mediatedmiR-27b transfection group). After a two-week osteogenic induction in vitro, staining was performed to assess the lipogenesis rate of the samples. In addition, ALP activity and PPARγ mRNA level was detected. The number of alizarin staining-positive osteogenic nodules and ALP activity (0.21±0.44 King unit) in group A was significantly diminished compared to those in group B, C, and D. Moreover, its lipogenesis rate (9.31±1.02%) and PPARγ mRNA level (1.86±0.39) were significantly higher than those in group B, C, and D (P < 0.05). The number of alizarin staining-positive osteogenic nodules and ALP activity (0.96±0.18 King unit) in group D were significantly reduced in comparison with those in groups B and C, while the lipogenesis rate (4.86±0.77%) and PPARγ mRNA level (0.93±0.34) were significantly higher than those in group B and C (P < 0.05) without difference between group B and group C (P > 0.05). Under a microgravity condition, liposomal nanoparticle-mediated miR-27b can impede the differentiation of BMSCs into osteoblasts via regulating PPARγ signal transduction.

CD33/CD10 Ratios in the Blast Region Evaluated by Flow Cytometry Predicts the Response of Chronic Myelogeneous Leukemia to Imatinib.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 2595-2595
Author(s):  
Satoko Oka ◽  
Masaki Mori
Keyword(s):  
Monoclonal Antibody ◽  
Bone Marrow ◽  
Flow Cytometry ◽  
Healthy Volunteers ◽  
Peripheral Blood ◽  
Chronic Myelogeneous Leukemia ◽  
Group A ◽  
One Year ◽  
Group B ◽  
Group D

Abstract Abstract 2595 Poster Board II-571 Background: The prediction of the response of chronic myelogeneous leukemia (CML) to imatinib is clinically important. However, no reliable markers are known. Methods: From January 2005 to April 2007, 32 patients with newly diagnosed CML were treated at our hospital. The diagnosis was made based on the existence of the Philadelphia chromosome. A routine bone marrow aspiration was performed to evaluate bone marrow smears, karyotypes, phenotypes, and bcr/abl message levels before the start of imatinib. Phenotypic analysis was conducted using three-color flow cytometry as follows: bone marrow mononuclear cells were stained with fluorescein isothiocyanate-conjugated monoclonal antibody, phycoerythrin-conjugated monoclonal antibody, and peridinin chlorophyll protein-conjugated CD45. A gate was set to identify blasts characterized by intermediate CD45 expression and low side-scatter properties. Phenotypes of the cells in the blast region were analyzed using a flow cytometer (FACSCalibur; BD Biosciences, San Jose, CA, USA). As a control, bone marrow samples were obtained from 8 healthy volunteers and 35 patients with refractory anemia of myelodysplastic syndrome (MDS-RA). Results: All patients were diagnosed as having CML in the chronic phase and given imatinib alone. According to the response after one year of treatment with imatinib, patients showing and not showing a complete cytogenetic response were designated as group A (26 patients) and group B (6 patients), respectively. There were no significant differences between the two groups in terms of the age, peripheral blood cell counts, peripheral blood blast percentages, peripheral blood basophil percentages, bone marrow cell differentials, bone marrow blast percentages, additional chromosomal abnormalities, Sokal's score, Hasford's score, and spleen size before the start of imatinib. The mean imatinib dose per day was higher in group A (349 mg) than in group B (284.4 mg); however, the results were not significant (p=0.051). Group B showed higher percentages of myeloid cells in the blast region than group A, while the former showed lower percentages of B lymphoid cells in the blast region than the latter. CD33/CD10, CD33/CD19, CD13/CD10, and CD13/CD19 ratios in the blast region in group B were significantly higher than in group A: 56.6 vs. 6.0 (p<0.01), 34.5 vs. 6.0 (p<0.01), 33.1 vs. 7.7 (p<0.01), and 23.8 vs. 4.9 (p<0.05), respectively. There were no differences between the two groups regarding the percentage of CD34+ and CD117+ cells. Similar results were obtained, when patients were evaluated after six months of treatment with imatinib. After the six-month imatinib treatment, patients were divided into two groups: one group (group C) showed undetectable bcr/abl messages (17 patients), and the other (group D) showed detectable bcr/abl messages (6 patients). There were no significant differences between the two groups in terms of imatinib dose per day and other clinical data. CD33/CD10, CD33/CD19, CD13/CD10, and CD13/CD19 ratios in the blast region in the group D and in the group C were 11.6 vs. 6.4 (p=0.05), 10.5 vs. 6.1 (p<0.05), 13.9 vs. 8.1 (p<0.05), and 9.4 vs. 5.7 (p<0.05), respectively. CD33/CD10, CD33/CD19, CD13/CD10, and CD13/CD19 ratios in the blast region in the MDS-RA patients and healthy volunteers were 3.2 vs. 0.7, 3.2 vs. 0.5, 2.5 vs. 0.6, and 3.0 vs. 0.6, respectively. Conclusion: An increase in CD33/CD10 ratios in the blast region in CML before the start of imatinib is associated with resistance to the drug. A cut-off value of 30 for CD33/CD10 ratios in the blast region in CML at diagnosis is useful for predicting the response of CML to imatinib after one year of treatment with the drug. Disclosures: No relevant conflicts of interest to declare.

Basement geology and Palaeozoic–Mesozoic mafic dykes from the Cape Meredith Complex, Falkland Islands: a record of repeated intracontinental extension

1997 ◽  
Vol 134 (3) ◽  
pp. 355-367 ◽  
Author(s):  
L. THISTLEWOOD ◽  
P. T. LEAT ◽  
I. L. MILLAR ◽  
B. C. STOREY ◽  
A. P. M. VAUGHAN
Keyword(s):  
Mantle Source ◽  
Lithospheric Mantle ◽  
Mafic Dykes ◽  
Lithospheric Extension ◽  
Mantle Sources ◽  
Group A ◽  
Group B ◽  
First Time ◽  
Group D ◽  
Island Basalt

Mafic dykes (Groups A–D) intruded into Mesoproterozoic basement amphibolites, gneisses, and granitoids of the Cape Meredith Complex on the southern tip of West Falkland, provide an important record of at least three periods of lithospheric extension during Palaeozoic and Jurassic times. Group A dykes are calc-alkaline lamprophyres that were generated by partial melting of an enriched subcontinental lithospheric mantle in Cambro-Ordovician times. Group B dykes are Ordovician dolerites derived from an asthenospheric mantle source, perhaps during the same extensional episode as Group A dykes. Group C dykes were also derived from an asthenospheric source and are possibly of Silurian age. The youngest, Group D, dykes are part of the widespread Jurassic Gondwana province. This group contains an oceanic island basalt-like sample and an enriched sample similar to both Group A lamprophyres and to the Jurassic Ferrar province in Antarctica. These correlations have interesting implications for the composition and evolution of mantle sources through time; the co-existence of Cambrian lamprophyres and Jurassic Ferrar-type magmas in the Cape Meredith Complex demonstrate for the first time that the enriched lithospheric mantle source postulated for the Ferrar magmas existed as far back as Cambrian times.

scholarly journals Effect of Long-Term Fungicide Applications on Virulence and Diversity of Colletotrichum spp. Associated to Olive Anthracnose

Plants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 311 ◽  
Author(s):  
Materatski ◽  
Varanda ◽  
Carvalho ◽  
Dias ◽  
Campos ◽  
...  
Keyword(s):  
Histone H3 ◽  
Lower Number ◽  
Chitin Synthase ◽  
Long Time ◽  
Group A ◽  
Colletotrichum Spp ◽  
Olive Trees ◽  
Group B ◽  
Group D

In this study, the presence and variability of Colletotrichum spp. was evaluated by comparing fungal isolates obtained from olive trees under long-time phytosanitary treatments with trees without any phytosanitary treatments (treated and untreated, respectively). Olive fruits of trees of the highly susceptible ‘Galega vulgar’ cultivar growing in the Alentejo region were used as samples. From the 210 olive trees sampled (half from treated and half from untreated orchards), 125 (59.5%) presented Colletotrichum spp., with a significant lower number of infected trees in treated (39) when compared to untreated orchards (86). The alignment and analysis of beta-tubulin (tub2), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), actin (ACT), chitin synthase (CHS-1) and histone H3 (HIS-3) gene sequences allowed the identification of all 125 isolates as belonging to the C. acutatum complex. The vast majority of the isolates (124) were identified as C. nymphaeae and one isolate, from an untreated tree, was identified as C. godetiae. Isolates were divided into five different groups: Group A: 39 isolates from treated trees matched in 100% with C. nymphaeae sequences from the database; Group B: 76 isolates from untreated trees matched in 100% with C. nymphaeae sequences from the database; Group C: one isolate from untreated trees presenting a single nucleotidic difference in the HIS-3 sequence; Group D: eight isolates from untreated trees presenting differences in two nucleotides in the tub2 sequences that changed the protein structure, together with differences in two specific nucleotides of the GAPDH sequences; Group E: one isolate, from untreated olive trees, matched 100% with C. godetiae sequences from the database in all genes. Considering the similarities of the sampled areas, our results show that the long-time application of fungicides may have caused a reduction in the number of olive trees infected with Colletotrichum spp. but an increase in the number of fruits positive to Colletotrichum spp. within each tree, which may suggest different degrees of virulence of Colletotrichum isolates from trees growing different management regimes. It is imperative that the fungicides described as causing resistance are applied at appropriate times and intervals, since their efficiency decreases when applied incorrectly and new and more virulent species may arise.

scholarly journals Global genetic diversity patterns and transmissions of SARS-CoV-2

2020 ◽  
Author(s):  
Zhi-wei Chen ◽  
Zhao Li ◽  
Hu Li ◽  
Hong Ren ◽  
Peng Hu
Keyword(s):  
Genetic Diversity ◽  
High Frequency ◽  
Economic Stress ◽  
Genome Sequences ◽  
Phylogenetic Tree Analysis ◽  
Diversity Patterns ◽  
Group A ◽  
Group B ◽  
First Time ◽  
Group D

AbstractBackgroundSince it was firstly discovered in China, the SARS-CoV-2 epidemic has caused a substantial health emergency and economic stress in the world. However, the global genetic diversity and transmissions are still unclear.Methods3050 SARS-CoV-2 genome sequences were retrieved from GIASID database. After aligned by MAFFT, the mutation patterns were identified by phylogenetic tree analysis.ResultsWe detected 17 high frequency (>6%) mutations in the 3050 sequences. Based on these mutations, we classed the SARS-CoV-2 into four main groups and 10 subgroups. We found that group A was mainly presented in Asia, group B was primarily detected in North America, group C was prevailingly appeared in Asia and Oceania and group D was principally emerged in Europe and Africa. Additionally, the distribution of these groups was different in age, but was similar in gender. Group A, group B1 and group C2 were declined over time, inversely, group B2, group C3 and group D were rising. At last, we found two apparent expansion stages (late Jan-2020 and late Feb-2020 to early Mar-2020, respectively). Notably, most of groups are quickly expanding, especially group D.ConclusionsWe classed the SARS-CoV-2 into four main groups and 10 subgroups based on different mutation patterns at first time. The distribution of the 10 subgroups was different in geography, time and age, but not in gender. Most of groups are rapidly expanding, especially group D. Therefore, we should attach importance to these genetic diversity patterns of SARS-CoV-2 and take more targeted measures to constrain its spread.

Survival of classic swine fever virus in hams made from the meat of pigs vaccinated with the PAV-250 strain and unvaccinated pigs

2019 ◽  
Vol 10 (3) ◽  
pp. 536-551
Author(s):  
Heidi Amezcua Hempel ◽  
María Salud Rubio Lozano ◽  
Eliseo Manuel Hernández Baumgarten ◽  
Pablo Correa Girón † ◽  
Oscar Torres Ángeles ◽  
...  
Keyword(s):  
Reference Strain ◽  
Clinical Signs ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Direct Immunofluorescence ◽  
Biometric Analysis ◽  
Treatment Groups ◽  
Group A ◽  
Group B ◽  
Group D

The study was to determine the presence of Classical Swine Fever virus (CSFv), in the meat of vaccinated pigs with the PAV-250 strain and then challenged using the same strain. Five treatment groups were established (each with four pigs). Group A: Pigs thatwere fed with processed hams from negative animals; Group B: Pigs that were fed with processed hams from commercial pigs inoculated with the ALD (reference strain) (titre of 104.0/ml); Group C: Pigs fed with processed hams from pigs infected with the virulent ALD strain (titre of 102.5/ml); Group D: Pigs fed with processed hams from pigs vaccinated with the PAV-250 strain and challenged with the ALD strain (titre of 101.1/ml); and Group E: Pigs fed with processed hams from pigs vaccinated with two doses of the PAV-250 strain and challenged with the ALD strain (negative). Blood samples were taken at d 1, 5, 10, 15 and 20 for biometric analysis. Groups B, C and D manifested clinical signs of CSFv: 40 °C temperature, anorexia, paralysis, vomiting, diarrhea, tremor, hirsute hair and cyanosis. Pigs were slaughtered and necropsies performed to identify lesions in tissues. Results of direct immunofluorescence testing of tissues were positive and the virus was recovered. Under these study conditions, it was found that CSFv resisted the cooking method at 68 °C for 40 min in hams from unvaccinated pigs, and that the virus was able to transmit the disease to healthy unvaccinated pigs, whereas the hams from the vaccinated animals did not transmit the virus.

scholarly journals The Role of Obesity in Predicting the Clinical Outcomes of COVID-19

Obesity Facts ◽  
2021 ◽  
pp. 1-9
Author(s):  
Serdar Sahin ◽  
Havva Sezer ◽  
Ebru Cicek ◽  
Yeliz Yagız Ozogul ◽  
Murat Yildirim ◽  
...  
Keyword(s):  
Clinical Outcomes ◽  
Health Care Providers ◽  
Study Groups ◽  
Normal Weight ◽  
Overweight And Obesity ◽  
Care Providers ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Group D

<b><i>Introduction:</i></b> The aim of this was to describe the predictors of mortality related to COVID-19 infection and to evaluate the association between overweight, obesity, and clinical outcomes of COVID-19. <b><i>Methods:</i></b> We included the patients &#x3e;18 years of age, with at least one positive SARS-CoV-2 reverse transcriptase-polymerase chain reaction. Patients were grouped according to body mass index values as normal weight &#x3c;25 kg/m<sup>2</sup> (Group A), overweight from 25 to &#x3c;30 kg/m<sup>2</sup> (Group B), Class I obesity 30 to &#x3c;35 kg/m<sup>2</sup> (Group C), and ≥35 kg/m<sup>2</sup> (Group D). Mortality, clinical outcomes, laboratory parameters, and comorbidities were compared among 4 groups. <b><i>Results:</i></b> There was no significant difference among study groups in terms of mortality. Noninvasive mechanical ventilation requirement was higher in group B and D than group A, while it was higher in Group D than Group C (Group B vs. Group A [<i>p</i> = 0.017], Group D vs. Group A [<i>p</i> = 0.001], and Group D vs. Group C [<i>p</i> = 0.016]). Lung involvement was less common in Group A, and presence of hypoxia was more common in Group D (Group B vs. Group A [<i>p</i> = 0.025], Group D vs. Group A [<i>p</i> &#x3c; 0.001], Group D vs. Group B [<i>p</i> = 0.006], and Group D vs. Group C [<i>p</i> = 0.014]). The hospitalization rate was lower in Group A than in the other groups; in addition, patients in Group D have the highest rate of hospitalization (Group B vs. Group A [<i>p</i> &#x3c; 0.001], Group C vs. Group A [<i>p</i> &#x3c; 0.001], Group D vs. Group A [<i>p</i> &#x3c; 0.001], Group D vs. Group B [<i>p</i> &#x3c; 0.001], and Group D vs. Group C [<i>p</i> = 0.010]). <b><i>Conclusion:</i></b> COVID-19 patients with overweight and obesity presented with more severe clinical findings. Health-care providers should take into account that people living with overweight and obesity are at higher risk for COVID-19 and its complications.

scholarly journals AB0422 LEFT VENTRICULAR ABNORMALITIES IN SYSTEMIC LUPUS ERYTHEMATOSUS PATIENTS FOLLOWED BY SEQUENTIAL ECHOCARDIOGRAPHY

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1510.1-1511
Author(s):  
T. Kuga ◽  
M. Matsushita ◽  
K. Tada ◽  
K. Yamaji ◽  
N. Tamura
Keyword(s):  
Lupus Erythematosus ◽  
Systolic Dysfunction ◽  
Left Ventricular ◽  
Monocyte Count ◽  
Lv Dysfunction ◽  
Group A ◽  
Group B ◽  
Lv Diastolic Dysfunction ◽  
Group D

Background:Cardiovascular disease (CVD) is detected in up to 50% of systemic lupus erythematosus (SLE) patients1and major cause of death2. Even clinically silent SLE patients can develop left ventricular (LV) diastolic dysfunction3. Proper echocardiographic follow up of SLE patients is required.Objectives:To clarify how the prevalence of LV abnormalities changes over follow-up period and identify the associated clinical factors, useful in suspecting LV abnormalities.Methods:29 SLE patients (24 females and 5 men, mean age 52.8±16.3 years, mean disease duration 17.6±14.5 years) were enrolled. All of them underwent echocardiography as the baseline examination and reexamined over more than a year of follow-up period(mean 1075±480 days) from Jan 2014 to Sep 2019. Patients complicated with pulmonary artery hypertension, deep venous thrombosis or pulmonary embolism and underwent cardiac surgery during the follow-up period were excluded. Left ventricular(LV) systolic dysfunction was defined as ejection fraction (EF) < 50%. LV diastolic dysfunction was defined according to ASE/EACVI guideline4. LV dysfunction (LVD) includes one or both of LV systolic dysfunction and LV diastolic function. Monocyte to HDL ratio (MHR) was calculated by dividing monocyte count with HDL-C level.Prevalence of left ventricular abnormalities was analysed at baseline and follow-up examination. Clinical characteristics and laboratory data were compared among patient groups as follows; patients with LV dysfunction (Group A) and without LV dysfunction (Group B) at the follow-up echocardiography, patients with LV asynergy at any point of examination (Group C) and patients free of LV abnormalities during the follow-up period (Group D).Results:At the baseline examination, LV dysfunction (5/29 cases, 13.8%), LV asynergy (6/29 cases, 21.7%) were detected. Pericarditis was detected in 7 patients (24.1%, LVD in 3 patients, LV asynergy in 2 patients) and 2 of them with subacute onset had progressive LV dysfunction, while 5 patients were normal in echocardiography after remission induction therapy for SLE. At the follow-up examination, LV dysfunction (9/29 cases, 31.0%, 5 new-onset and 1 improved case), LV asynergy (6/29 cases, 21.7%, 2 new-onset and 2 improved cases) were detected. Though any significant differences were observed between Group A and Group B at the baseline, platelet count (156.0 vs 207.0, p=0.049) were significantly lower in LV dysfunction group (Group A) at the follow-up examination. Group C patients had significantly higher uric acid (p=0.004), monocyte count (p=0.009), and MHR (p=0.003) than Group D(results in table).Conclusion:LV dysfunction is progressive in most of patients and requires regular follow-up once they developed. Uric acid, monocyte count and MHR are elevated in SLE patients with LV asynergy. Since MHR elevation was reported as useful marker of endothelial dysfunction5, our future goal is to analyse involvement of monocyte activation and endothelial dysfunction in LV asynergy of SLE patients.References:[1]Doria A et al. Lupus. 2005;14(9):683-6.[2]Manger K et al. Ann Rheum Dis. 2002 Dec;61(12):1065-70.[3]Leone P et al. Clin Exp Med. 2019 Dec 17.[4]Nagueh SF et al. J Am Soc Echocardiogr. 2016 Apr;29(4):277-314.[5]Acikgoz N et al. Angiology. 2018 Jan;69(1):65-70.Numbers are median (interquartile range), Mann-Whitney u test were performed, p value less than 0.05 was considered statistically significant.Disclosure of Interests: :None declared

scholarly journals Prophylactic temporary abdominal aortic balloon occlusion for patients with pernicious placenta previa: a retrospective study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Fei Huo ◽  
Hansheng Liang ◽  
Yi Feng
Keyword(s):  
Placenta Previa ◽  
Balloon Occlusion ◽  
Artery Embolism ◽  
Abdominal Aortic ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Clinical Records ◽  
Group D ◽  
Central Placenta Previa

Abstract Background Pernicious placenta previa (PPP) can increase the risk of perioperative complications. During caesarean section in patients with adherent placenta, intraoperative blood loss, hysterectomy rate and transfusion could be reduced by interventional methods. Our study aimed to investigate the influence of maternal hemodynamics control and neonatal outcomes of prophylactic temporary abdominal aortic balloon (PTAAB) occlusion for patients with pernicious placenta previa. Methods This was a retrospective study using data from the Peking University People’s Hospital from January 2014 through January 2020. Clinical records of pregnant women undergoing cesarean section were collected. Patients were divided into two groups: treatment with PTAAB placement (group A) and no balloon placement (group B). Group A was further broken down into two groups: prophylactic placement (Group C) and balloon occlusion (group D). Results Clinical records of 33 cases from 5205 pregnant women underwent cesarean section were collected. The number of groups A, B, C, and D were 17, 16, 5 and 12.We found that a significant difference in the post-operative uterine artery embolism rates between group A and group B (0% vs.31.3%, p = 0.018). There was a significant difference in the Apgar scores at first minute between group A and group B (8.94 ± 1.43 vs 9.81 ± 0.75,p = 0.037),and the same significant difference between two groups in the pre-operative central placenta previa (29.4% vs. 0%,p = 0.044), complete placenta previa (58.8% vs 18.8%, p = 0.032),placenta implantation (76.5% vs 31.3%, p = 0.015). We could also observe the significant difference in the amount of blood cell (2.80 ± 2.68vs.10.66 ± 11.97, p = 0.038) and blood plasma transfusion (280.00 ± 268.32 vs. 1033.33 ± 1098.20, p = 0.044) between group C and group D. The significant differences in the preoperative vaginal bleeding conditions (0% vs 75%, p = 0.009), the intraoperative application rates of vasopressors (0% vs. 58.3%, p = 0.044) and the postoperative ICU (intensive care unit) admission rates (0% vs. 58.3%, p = 0.044) were also kept. Conclusions PTAAB occlusion could be useful in reducing the rate of post-operative uterine artery embolism and the amount of transfusion, and be useful in coping with patients with preoperative vaginal bleeding conditions, so as to reduce the rate of intraoperative applications of vasopressors and the postoperative ICU (intensive care unit) admission. In PPP patients with placenta implantation, central placenta previa and complete placenta previa, we advocate the utilization of prophylactic temporary abdominal aortic balloon placement.

scholarly journals Biomechanical study of Tile C3 pelvic fracture fixation using an anterior internal system combined with sacroiliac screws

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Lin Liu ◽  
Donggui Zeng ◽  
Shicai Fan ◽  
Yongxing Peng ◽  
Hui Song ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Steel Plate ◽  
Standing Position ◽  
Pelvic Fractures ◽  
Internal Fixator ◽  
Sacroiliac Joints ◽  
Sacroiliac Screws ◽  
Group A ◽  
Group B ◽  
Group D

Abstract Background How to perform minimally-invasive surgery on Tile C pelvic fractures is very difficult, and it is also a hot topic in orthopedic trauma research. We applied minimally-invasive treatment using an anterior internal fixator combined with sacroiliac screws. Objectives To compare the biomechanical properties of different fixation models in pelvic facture specimens, using an internal fixation system or a steel plate combined with sacroiliac screws. Methods Sixteen fresh adult cadaver pelvic specimens were randomly separated into four groups named A, B, C, and D. The four groups were respectively stabilized using a two-screwed, three-screwed, or four-screwed anterior internal fixator or a steel plate with sacroiliac screws. All models were tested in both standing and sitting positions. Vertical loads of 600 N were applied increasingly. Shifts of bilateral sacroiliac joints and pubis rupture were measured. Results The shifts in sacroiliac joints and pubis rupture in the standing position were all less than 3.5 mm, and the shifts in the sitting position were all less than 1 mm. In the standing position, the results of shifts in the sacroiliac joints were group C < group D < group B < group A. For comparisons between A:B and C:D, P > 0.05. For comparisons between A, B:C, and D, P < 0.05. The results of shifts in pubis ruptures were group D < group C < group B < group A. In the comparison between C:D, P > 0.05; for comparisons between A:B, A:C, A:D, B:C, and B:D, P < 0.05. In the sitting posture, the results of shifts in the sacroiliac joints were group C < group D < group B < group A, and the shifts in the pubis ruptures were group D < group C < roup B < group A. For comparison between C:D, P > 0.05. For comparisons between A:B, A:C, A:D, B:C, and B:D, P < 0.05. Conclusion Use of an anterior internal fixator combined with sacroiliac screws effectively stabilized Tile C3 pelvic fractures. The stability of specimens increased as the number of screws in the internal fixator increased.

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