Neuroprotective Activity ofSibjeondaebo-tangon AβPeptide-Induced Damages
Background.Sibjeondaebo-tang(SJDBT) has been used to treat diverse disorders including neuropsychiatric disabilities in traditional Korean medicine.Objective. The present study aims to investigate the potential effects of SJDBT on neuroprotection against Aβ peptide-induced damage usingin vitroculture andin vivorat brain systems.Materials and Methods. PC12 cell viability was analyzed by MTT assay, and neurite arborizations and caspase 3 protein signals in cultured PC12 cells andin vivocortical neurons were analyzed by immunofluorescence staining. Phospho-Erk1/2 protein was analyzed by immunofluorescence staining and western blot analysis.Results. In PC12 cells, atrophied cell body and reduced neurite extension by Aβtreatment were recovered by SJDBT treatment. Caspase 3 protein signals were increased in Aβ-treated PC12 cells, but SJDBT treatment decreased apoptotic cell death. Caspase 3 activation in cortical neurons, which was induced similarly by Aβtreatment, was reduced by SJDBT treatment. Furthermore, phospho-Erk1/2 protein levels, which had been decreased by Aβtreatment, were elevated in the cortical neurons by SJDBT treatment.Conclusion. These data show that SJDBT may play a role in protecting from damages induced by Aβin neuronal tissue and further suggest that SJDBT can be explored as the potential therapeutic target for AD treatments in human.