Antiulcer Activity of Indigenous PlantOperculina turpethumLinn.

In the Indian traditional system of medicineOperculina turpethumis commonly used to treat various ailments including peptic ulcer, inflammation, and pain. Ulcer preventive and ulcer protective activities of HAOP and MOP stem bark extracts ofOperculina turpethum(100 mg/kg, b.w., orally) were evaluated employing aspirin + pylorus ligation (APL) model in experimental rats. The results suggested that both extracts (HAOP and MOP) possess enhanced ulcer preventive and protective activities when compared with the standard drug ranitidine. HAOP showed more pronounced effect when compared to MOP. Further the result of the histopathological and biochemical studies also confirms potent ulcer preventive and protective nature of a extracts in a similar manner.

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ANTIULCER AND ANTI-INFLAMMATORY ACTIVITIES OF HYDROALCOHOLIC EXTRACTS OF STEM BARK AND LEAF OF ANOGEISSUS PENDULA EDGEW

INDIAN DRUGS ◽

10.53879/id.58.01.11950 ◽

2021 ◽

Vol 58 (01) ◽

pp. 41-46

Author(s):

Deeksha Singh ◽

◽

Amit Nayak ◽

Rakesh Yadav ◽

Uttam Singh Baghel ◽

...

Keyword(s):

Phenolic Compounds ◽

Stem Bark ◽

Inflammatory Activity ◽

Antiulcer Activity ◽

Paw Edema ◽

Ulcer Index ◽

Pylorus Ligation ◽

Anti Inflammatory ◽

Anogeissus Pendula

Anogeissus pendula Edgew has been reported to be used in gastric disorder even though no attempt has been made to evaluate the same. The present study was designed to evaluate the hydroalcoholic extracts of stem bark and leaves for in vivo acute antiulcer and anti-inflammatory activities. Antiulcer activity was studied by pylorus ligation induced ulcers while anti-inflammatory activities was studied by carrageenan induced paw edema. The extract of stem bark at 200 and 400 mg/kg b. w., significantly (P<0.05) reduced ulcer index whereas extracts of both stem bark and leaves significantly (P<0.001) reduced paw diameter at the fifth hour. The hydroalcholic extract of stem bark of A. pendula possesses more significant antiulcer and anti-inflammatory activity than the hydroalcholic extract of leaves may be due to the presence of higher amount of phenolic compounds.

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Comparative Evaluation of Antiulcer Activity of Ficus religiosa (Stem Bark) Extracts Prepared by Different Methods of Extraction

Current Traditional Medicine ◽

10.2174/2215083805666190328210119 ◽

2020 ◽

Vol 6 (4) ◽

pp. 351-359

Author(s):

Sunita Panchawat ◽

Joohee Pradhan

Keyword(s):

Extraction Method ◽

Soxhlet Extraction ◽

Stem Bark ◽

Antiulcer Activity ◽

Ulcer Index ◽

Ficus Religiosa ◽

Standard Drug ◽

Hydroalcoholic Extract ◽

Acute Toxicity Study ◽

Assisted Extraction

Background: The stem bark of Ficus religiosa L. (Family-Moraceae) is traditionally used to treat gonorrhea, diarrhea, dysentery, leucorrhea, menorrhagia, vaginal and other urogenical disorders, ulcers and gastrohelcosis. Objective: The study was aimed at comparing antiulcer activity (ethanol induced gastric ulcer) for stem bark extracts of Ficus religiosa Linn. prepared by different extraction methods viz. soxhlet, ultrasonication and microwave assisted extraction. Methods: The powdered drug material was extracted by soxhlet (time 48 hours., temperature 60°C and solvent 60% ethanol), Ultrasonic (90 min., 50°C and acetone as solvent)and Microwave (3 min., 60°C and acetone as solvent) assisted extraction method. The acute toxicity study was carried out in adult female albino rats by “fix dose” method of OECD guideline no. 420 for selection of doses. Antiulcerogenic effect of prepared extracts (200mg/kg) was evaluated by ethanol induced ulcer method using omeprazole (40 mg/kg) as standard drug and absolute alcohol 99.5% as necrotizing agent. Result and Conclusion: Acute toxicity study did not show any significant toxicity signs and extracts were said to be practically non-toxic at tested dose level of 2000 mg/kg, b.w. The best antiulcer activity was shown by the hydroalcoholic extract prepared by soxhlet extraction method. Administration of ethanol produced significant ulcer index (41.00 ± 1.75) in the control group. Standard drug omeprazole (40 mg/kg) produced percentage protection of 70.31%. Among all three extracts, Ulcer index is significantly (p<0.001) protected by hydroalcoholic extract (Soxhlet Extraction Method) with percentage protection 52.02%.

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Evaluation of Antiulcer Activity of 80% Methanol Extract and Solvent Fractions of the Root of Croton macrostachyus Hocsht: Ex Del. (Euphorbiaceae) in Rodents

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/2809270 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11

Author(s):

Alefe Norahun Mekonnen ◽

Seyfe Asrade Atnafie ◽

Mohammedbirhan A. Wahab Atta

Keyword(s):

Peptic Ulcer ◽

Ethyl Acetate ◽

Crude Extract ◽

Antiulcer Activity ◽

Chloroform Fraction ◽

Sprague Dawley ◽

Aqueous Fraction ◽

Pylorus Ligation ◽

Croton Macrostachyus ◽

Ulcer Model

Background. Peptic ulcer disease causes significant mortality and morbidity. Plant kingdom provides a useful source for the development of new antiulcer agents. Croton macrostachyus is traditionally used to treat peptic ulcer in Ethiopia. This study aimed to evaluate the antiulcer activity of C. macrostachyus root extracts in rodents using different models. Methods. The crude extract was obtained by cold maceration in 80% methanol and fractionated with chloroform, ethyl acetate, and distilled water. The antiulcer activity was evaluated using pylorus ligation-induced ulcer model in Sprague Dawley rats and acidified ethanol-induced ulcer model in Swiss albino mice. The test groups received three doses (100, 200, and 400 mg/kg) of the crude extract and fractions for 7 days before induction of ulcer. Positive controls received omeprazole 30 mg/kg for the pylorus ligation-induced ulcer model and sucralfate 100 mg/kg for the acidified ethanol-induced ulcer model. Negative controls received vehicle (2% tween 80). Results. The crude hydromethanolic extract of C. macrostachyus showed significant (p<0.05) antiulcer activity on both pyloric ligation and HCl/ethanol-induced ulcer in rats and mice. It has antisecretary effect (p<0.001) as well. All three administered doses of chloroform fraction (p<0.05) and only higher doses of ethyl acetate fraction (p<0.05) possessed significant antiulcer activity. In contrast, the aqueous fraction did not have significant antiulcer effect at all tested doses. Conclusion. The present study demonstrated that the crude extract, chloroform, and ethyl acetate fractions possessed significant dose-dependent antiulcer activity.

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Evaluation of In Vivo Antiulcer Activity of Hydro-Methanol Extract and Solvent Fractions of the Stem Bark of Ficus thonningii (Moraceae) on Rodent Models

BioMed Research International ◽

10.1155/2021/6685395 ◽

2021 ◽

Vol 2021 ◽

pp. 1-10

Author(s):

Habtalem Adane ◽

Seyfe Asrade Atnafie ◽

Zemene Demelash Kifle ◽

Digambar Ambikar

Keyword(s):

Methanol Extract ◽

Stem Bark ◽

Traditional Healers ◽

Gastric Volume ◽

Antiulcer Activity ◽

Ulcer Disease ◽

Pylorus Ligation ◽

The Social ◽

Ficus Thonningii

Introduction. The stem bark of Ficus thonningii is used by Ethiopian traditional healers and the community for the treatment of peptic ulcer disease. Thus, the current study was aimed at evaluating the antiulcer effect of hydro-methanol extract and solvent fractions of F. thonningii. Methods. The stem bark of F. thonningii was collected and shed dried. Then, the stem bark was extracted by 80% hydro-methanol solvents and dried. The part of the dried hydro-methanol extract was further fractionated with n-hexane, chloroform, and distilled water. Dose-dependent pylorus ligation, curative indomethacin-induced, and time-dependent ethanol-induced ulcer models were evaluated for the hydro-methanol extract and solvent fractions. Statistical analysis was done by using the Statistical Package for the Social Sciences (SPSS) version 24. The analyses were carried out using one-way analysis of variance (ANOVA), followed by Tukey’s multiple comparison tests. The result was considered significant when p < 0.05 . Results. The extract of F. thonningii showed a significant ( p < 0.001 ) reduction in total acidity at all the tested doses (100, 200, and 400 mg/kg). All the tested doses of the hydro-methanol extract significantly reduced the gastric volume as compared to the vehicle (NC) ( p < 0.01 ). The gastric pH was significantly ( p < 0.05 ) increased by 200 and 400 mg/kg. Similarly, 200 mg/kg and 400 mg/kg significantly ( p < 0.05 ) lowered gastric ulceration as compared to the NC. The hydro-methanol extract and aqueous fractions of F. thonningii at 200 mg/kg showed significant ( p < 0.05 ) reduction in the ulcer index on a repeated dose of the hydro-methanol and solvent fractions. Ulcer healing effect on indomethacin-induced ulcer was not significant ( p > 0.05 ) for all tested doses of the hydro-methanol extract. Conclusion. The study demonstrated that the stem bark of F. thonningii has a potential antiulcer activity that might be due to antisecretory or cytoprotective effects.

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An ayurvedic tonic formulation and investigations for its antiulcer activity

International Journal of Novel Trends in Pharmaceutical Sciences ◽

10.26452/ijntps.v9i3.1251 ◽

2020 ◽

Vol 9 (3) ◽

pp. 39-42

Author(s):

Ravichandran S ◽

Bhavani J ◽

Satheesh Kumar D ◽

Chandrasekaran A R ◽

Saraladevi V ◽

...

Keyword(s):

Peptic Ulcer ◽

Free Radicals ◽

Large Population ◽

Antiulcer Activity ◽

Standard Drug ◽

Ulcer Disease ◽

Pain Medications ◽

Stomach Pain ◽

The World ◽

Current Scenario

The ulcers are complications, and as a disease, it is called PUD (Peptic Ulcer Disease). In the current scenario, a large population around the world are suffering from the ulcers, which are ranging from 25-55 years the age. This is not so dangerous as such but can become most dreadful when untreated or unattended. PUD can become the causes for other diseases like malnutrition, stomach pain etc. there are a lot of factors that are responsible for causing an ulcer. Few of them are the prolonged use of pain medications, malnutrition, stress and free radicals. There are also medicinal plants that are used to treat ulcers and effectively cure them without causing significant side effects. Various research publications prove the antiulcer property of the herbs and plants. The ayurvedic tonic formulation was prepared using the herbs and sugar base. The formulation was investigated for the antiulcer activity in the Ethanol-induced ulcers method. The antiulcer activity was investigated using two doses and compared to the ayurvedic standard marketed formulation and the standard drug omeprazole.

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Gastroprotective effect of leaves of Breyniavitis-Idaea

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i2.2247 ◽

2020 ◽

Vol 11 (2) ◽

pp. 2519-2524

Author(s):

Poojitha M ◽

Saravanakumar A S ◽

Satyanarayana S V

Keyword(s):

Side Effects ◽

Chemical Constituents ◽

Gastric Volume ◽

Gastric Ulcers ◽

Antiulcer Activity ◽

Standard Drug ◽

Synthetic Drugs ◽

Pylorus Ligation ◽

Dose Dependent ◽

Ligation Method

Due to the lure backs of synthetic drugs that are being used medical field has turned over to the traditional medicine which are devoid of side effects and major adverse effects. So that Herbal drugs were seen as probable replacements for the handling of PUD without showing side effects and equaling the treatment efficacy. The literature review on investigations of antiulcer activity of various plant drug, the present investigation was carried out to investigate the antiulcer potential, chemical constituents present in the methanol extract of traditional plant Breyniavitis-idaea. Gastric ulcers in experimental animals were brought by four different models like Ethanol(Alcohol) induced, NSIDS (Indomethacin) induced, Pylorus ligation method and Cold resistant stress induced method by comparing with the standard drug namely omeprazole (20mg/kg) which exhibited the dose dependent capacity of the extract (125mg/kg,250mg/kg, 500mg/kg) and also the biochemical parameters like ALP, GSH, pH and Gastric volume contents were estimated in all the selected groups (Design of Experiment). The results obtained from the study has helped to identify that 500mg/kg of plant extract has gastro protective effect in all the chosen models in comparison to the omeprazole (20mg/kg) standard drug. Owing to the prevalence of different phytoconstituents like poly phenols and flavonoids shown the dose dependent potent gastroprotective activity.

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Studies on the Antiulcer Activity of the Chloroform Fraction of Balanites aegyptiaca Stem Bark Extract

Annals of Basic and Medical Sciences ◽

10.51658/abms.202121.9 ◽

2021 ◽

Vol 2 (1) ◽

Author(s):

Chinenye J. Ugwah-Oguejiofor ◽

Oguejiofor M. Ugwah ◽

Emmanuel U. Etuk ◽

Shuaibu O. Bello ◽

Adamu A. Aliero

Keyword(s):

Stem Bark ◽

Tween 20 ◽

Bark Extract ◽

Antiulcer Activity ◽

Phytochemical Analysis ◽

Chloroform Fraction ◽

Balanites Aegyptiaca ◽

Standard Drug ◽

Stem Bark Extract ◽

Group 2

Background: Balanites aegyptiaca L. Delile (Zygophyllaceae) is a perennial tree found mostly in the desert environments. Decoctions of its stem barks are used traditionally in northern Nigeria to treat wounds, ulcers and stomach ailments. Other ethnomedicinal uses include: treatment of malaria, pain and fever. The aim of this study was to evaluate the antiulcer activity of the chloroform stem bark extract of Balanites aegyptiaca in Wistar rats. Materials and methods: Acute toxicity study on the chloroform fraction of the stem bark extract of Balanites aegyptiaca was carried out using the OECD 425 guidelines at a limit dose of 3000 mg/kg. Phytochemical analysis of the fraction was carried out to detect the presence of alkaloids, tannins, flavonoids, steroids, carbohydrates, saponins and glycosides using standard procedures. The antiulcer activity of the fraction was evaluated in ethanol and indomethacin-induced ulcer models in rats. Rats were allotted into five groups (n=5). Group 1 received 10% Tween 20 (1 ml/kg), group 2 received standard drug, omeprazole (20 mg/kg), and groups 3-5 were the extract groups and received 125, 250 and 500 mg/kg, p.o. respectively of the chloroform fraction of Balanites aegyptiaca. The mean ulcer indices were assessed and the percentage ulcer inhibition calculated. Results: At the limit dose of 3000 mg/kg the fraction of Balanites aegyptiaca did not produce any mortality or observable signs of toxicity in the rats. The phytochemical analysis showed the presence of flavonoids, saponins and steroids. The fraction at the doses of 250 and 500 mg/kg caused a significant (p<0.05) reduction in mean ulcer indices in the ethanol and indomethacin-induced ulcer models when compared to the control. Conclusion: Our study showed that the chloroform fraction of the stem bark extract of Balanites aegyptiaca possesses antiulcer activities. Further studies are needed to isolate the active constituents.

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In- vivo diuretic and antiulcer activity in fruits of Buchanania lanzan

International Journal of Phytomedicine ◽

10.5138/09750185.1920 ◽

2017 ◽

Vol 9 (4) ◽

pp. 673

Author(s):

Chandra Kishore Tyagi ◽

Atul Tripathi ◽

Gyanendra Singh ◽

Amol Chandekar ◽

Sunil Sahu

Keyword(s):

Urine Volume ◽

Control Group ◽

Albino Rats ◽

Antiulcer Activity ◽

Diuretic Activity ◽

Standard Drug ◽

Pylorus Ligation ◽

Dose Dependent Increase ◽

Dose Dependent

Ethnopharmacological relevance: The fruits of Buchanania lanzan are used traditionally in the treatment of skin afflictions, sores, ulcers, inflammation and as diuretic (1,21,35,42). The aim of the study was to investigate the antiulcer and diuretic activity of the crude ethanolic and pet. ether extract from the fruits of Buchanania lanzan.Materials and methods: The antiulcer activity of the crude extracts was evaluated in ethanol and pylorus ligation induced model for gastric lesions in Wister albino rats (2,8,12,19). Parameters such as gastric volume, pH and acidity were determined in the pylorus ligation model. Furthermore the diuretic activity was evaluated in comparison of Standard drug i.e. Furosemide.Results: The acute toxicity studies revealed that LD50> 2000mg/kg for the extract. The extract caused a significant (p<0.05) dose-dependent inhibition of ulcer in the ethanol and pylorus ligation induced ulcer models at the dose of 250mg/kg, respectively. And the diuretic activity of extract also found significant (p<0.01) dose dependent increase in urine volume. Both ethanol and aqueous extracts have showed a significant dose-dependent increase in the excretion of electrolytes when compared to the control group.Conclusion: Our data provide a rational base for the folkloric use of Buchanania lanzan in the treatment of ulcers and as diuretic.

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Antiulcerogenic effect of Methanolic Root Extract of Berberis lycium Linn by Pylorus ligation and Ethanol induced Ulceration

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v11i4-s.4966 ◽

2021 ◽

Vol 11 (4-S) ◽

pp. 9-14

Author(s):

Priyanka Namdeo ◽

Madhuri Pandole ◽

Priti Singh

Keyword(s):

Gastric Volume ◽

Albino Rats ◽

Antiulcer Activity ◽

Root Extract ◽

Total Acidity ◽

Free Acidity ◽

Ulcer Index ◽

Standard Drug ◽

Pylorus Ligation ◽

Berberis Lycium

Background: Gastric ulcer is one of the most prevalent gastrointestinal disorders, which affects approximately 5-10% of people during their life. In recent years, abundant work has been carried out on herbal medicine to clarify their potential efficacy in gastric ulcer prevention or management. The present study was carried out to evaluate the antiulcer activity of the methanolic root extract of Berberis lycium in albino rats. Method: The methanolic root extract of Berberis lycium was prepared by hot extraction method. Anti-ulcer activity was evaluated and method employed was pylorus ligation and ethanol induced in albino rats. Preliminary methanolic extract of Berberis lycium was subjected to the acute oral toxicity study according to the OECD guideline no. 425. Animals were divided into four groups of six animals each. The animals of Group I served as normal control (vehicle) which received distilled water. Group II and III received 250 mg/kg and 500 mg/kg of methanolic root extract, respectively. In pylorus ligation induced ulcer model, various parameters were studied viz. gastric volume, pH, total acidity, free acidity, and ulcer index. Ulcer index and percentage inhibition of ulceration was determined for ethanol induced ulcer model. Group IV received Ranitidine at 50 mg/kg was used as the standard drug. Pretreatment of methanol root extract of Berberis lycium showed significant (P˂0.05) decrease in the gastric volume, total acidity and free acidity. However, pH of the gastric juice was significantly increased only at higher dose 500 mg/kg. It showed also significant (P˂0.05) decrease in number of ulcers and ulcer score index in pylorus ligation and ethanol induced ulceration models. Results: The methanol root extract of Berberis lycium showed a significant reduction in the total acidity, free acidity and acid volume. The efficacy of plant extract at high dose was comparable with the standard drug Ranitidine. Conclusion: Our study results support the ethnomedical use of root of Berberis lycium. Keywords: Antiulcer activity, Berberis lycium, Pylorus ligation, Ranitidine, Ulcer Index.

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Evaluation of the Antiulcer Activity of Ethanolic Leave Extracts of Saraca indica against Ethanol, Pylorus Ligature and Indomethacin Induced Ulcer in Albino Rats

Current Bioactive Compounds ◽

10.2174/1573407216666200109142305 ◽

2020 ◽

Vol 16 (8) ◽

pp. 1191-1196

Author(s):

Pritt Verma ◽

Shravan K. Paswan ◽

Vishal K. Vishwakarma ◽

Priyanshi Saxena ◽

Chandana V. Rao ◽

...

Keyword(s):

Mucosal Injury ◽

Gastric Volume ◽

Gastric Ulcers ◽

Albino Rats ◽

Antiulcer Activity ◽

Cellulose Solution ◽

Total Acidity ◽

Ulcer Index ◽

Standard Drug ◽

High Doses

Background: To evaluate the antiulcer activity of ethanolic leaves extract of Saraca indica against ethanol, pylorus ligature and indomethacin in albino rats. Materials and Methods: Ulcer was produced by ethanol, pylorus ligature and indomethacin in albino rats. Five groups (n=6) of rats were orally pre-treated with carboxymethyl cellulose solution, and ranitidine (80 mg/kg) respectively. In ethanol induced ulcer, the animals were treated with 200 and 400 mg/kg b.w. ethanolic leave extracts of Saraca indica in 0.3% CMC solution, 60 minutes before oral administration of absolute ethanol to produce gastric mucosal injury. In indomethacin induced ulcer, the drug was administered orally at the dose of 30 mg/kg b.w. After 7-9 hours of administration of indomethacin (30 mg/kg); the animals were sacrificed with high doses of anesthesia. In the pylorus ligature method, volume of free acidity, gastric secretion, pH and total acidity were estimated. In all three models, the ulcer index and % protection were estimated. Results: The anti ulcer activity of ethanolic leave extracts of Saraca indica in ethanol, indomethacin and pylorus ligature models is evident from the significant (P<0.001) reduction in ulcer index. In pylorus ligature model, significant (P<0.001) reduction in total acidity gastric volume and increase in pH were observed when compared with the standard drug. Conclusion: Ethanolic leave extracts of Saraca indica were found to be significantly protective against ethanol, indomethacin and pylorus ligature induced gastric ulcers in the experimental albino rats. The result obtained suggest that ethanolic leave extracts of Saraca indica possesses significant anti-ulcer activity.

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